TataKelola dan KamSiber Kecerdasan Buatan v022.pdf
Lecture 2 introduction to bioprocess
1. Introduction to Bioprocess 12th. July 2010 CEPP, UTM Skudai, Johor Prof. Dr. Hesham A. El Enshasy Faculty of Chemical Engineering CEPP, UTM, Skudai, Malaysia
2. The oldest Known Biotech. Protocol for Yeast production Bread and Beer Manufacturing Process, the 5th. Dynastry (ca 2400 BC) Leiden Egyptian Museum, Holland
8. Determination of process bottleneck(s) Bioprocess Optimization in Lab. scale (Development of cultivation strategy to reach the maximal productivity) Scaling up of the Process Down Stream (Separation/Isolation/Purification and increase of product Stability)
9. Substrate Input and Output Oxygen Carbon dioxide Carbon and Energy Sources Biomass CELL Metabolite(s) Nitrogen Source Water Other requirements (P, S,Na,K,Mg,etc…) Heat
10. General Requirements for Medium Composition It will Produce the maximal yield of product(s) or biomass per gram of substrate used (High Yield Coefficient) It will produce the maximal concentration of product or biomass (High volumetric production) It will permit the maximal yield of product formation (Maximal productivity) There will be the minimal yield of undesirable products It will be of consistent quality and be readily available throughout the year It will cause minimal problems during media preparation and sterilization It will cause minimal problems in other aspects of the production process particularly Aeration and agitation, extraction and waste treatment
14. What can we use Petri dish for Strain Isolation and Identification work As first step for inoculum propagation Sterility testing Rapid screening for certain microbial metabolites Antimicrobial sensitivity testing Short term strain preservation
15. What can we use Shake flask for Primary production of certain metabolites Genetic material preparation (cell mass production for DNA isolation) Medium optimization (C-, N-, P- and elements-sources and concentrations) Cultivation conditions optimization (Temperature, pH) Primary understanding of oxygen and mixing requirements through the change in (Shaking intensity, Shaking eccentricity, working volume and polymer addition) Primary data for Growth and product formation kinetics
20. Why Plant Cell Bioreactor ? It is independent of geographical and seasonal variations and various environmental factors. It offers a defined production system, which ensure continuous supply of products, uniform quality and yield It is possible to produce novel compounds that are not normally found in parent plant. It is independent of political interface Efficient downstream recovery with low cost and minimum number of steps. High efficient production rate with significant short production time Fully Compliance to cGMP requirements for Biopharmaceuticals
