Lecture 3 bioprocess control

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Lecture 3 bioprocess control

  1. 1. Bioprocess Control<br />(On-line, off-line and in-line)<br />14th. July 2010<br />CEPP, UTM Skudai, Johor<br />Prof. Dr. Hesham A. El Enshasy<br />Faculty of Chemical Engineering<br />CEPP, UTM, Skudai, Malaysia <br />
  2. 2. Main Items of Presentation<br />Cell Input and Cell out-put (conditions and nutrients requirements)<br />Parameters for measurements and control for cultivation system<br />Sensors used for bioreactors <br />Feed back loops for bioprocess control <br />Summary screen for different control system <br />SCADA system for control using ethernet platform <br />
  3. 3. Cell In-put and Cell Out-put<br />
  4. 4. Substrate(s) Input and Output<br />Oxygen<br />Carbon dioxide <br />Carbon and Energy <br />Sources <br />Biomass<br />CELL<br />Metabolite(s)<br />Nitrogen Source<br />Water <br />Other requirements<br />(P, S,Na,K,Mg,etc…)<br />Heat<br />
  5. 5. Different Parameters for cultivation system<br />
  6. 6. Integrated of Mathematical Methods for Advanced Process Control <br />Ref. (Clementschitsch and Bayer. Microbial Cell Factories 5:19 (2006) <br />
  7. 7. Input and Output of Bioreactor system (General overview)<br />
  8. 8. Main in situ sensors used for measuring cultivation parameters<br />
  9. 9. Exhaust gas<br />Analyzer<br />Feed pump(s)<br />pH<br />Temperature<br />DO<br />Aeration<br />Pressure<br />Power consumption<br />Weight / volume<br />Stirrer Speed<br />Measurement and open or closed loop control<br />Measurement only<br />Common measurement and control of bioreactors as <br />generally accepted as routine equipment<br />
  10. 10.
  11. 11. Lower ports for in situ sterilizable STR<br />
  12. 12. Upper/lower ports and Headplate<br />
  13. 13. pH values of different liquids <br />
  14. 14. pH and hydrogen ion concentration <br />
  15. 15. pH measuring circuit and pH meter construction<br />
  16. 16. Temperature Effect on pH value <br />
  17. 17. Temperature Error Table <br />
  18. 18. DO meter construction<br />
  19. 19. The relation between DO and Temperature<br />
  20. 20. Standard Antifoam Sensor<br />Type of foam:<br /> Early foaming<br /> Late foaming<br />Antifoam effects on:<br /> DO<br /> Growth morphology<br /> (Filamentous MO)<br />Foam sensors:<br /> Low foam sensor<br /> High foam sensor<br />
  21. 21. On-line cell mass determination methods<br />
  22. 22. On-line cell mass and viability measurement<br />Theory: <br />Cells with intact plama membranes act like tiny capacitors under the influence of electric field. The nonconducting nature of plasma membranes allows charge to build up. The resulting capacitance can be measured and is usually expressed in picofarad per centimeter (pF/cm). It depends on the cell type and is directly proportional to membrane bound volume of viable cells. <br />
  23. 23. Controlling stat-culture<br />
  24. 24. Feedback and Feedforward controllers<br />Feedback control:A control algorithm to reduce the error between the set point and the controlled variable (most often PID or model predictive controller algorithm is used)<br />Feedforward control: A computation of the manipulated variable from a measurement of the disturbance (most often corrected by a PID or model predictive controller)<br />
  25. 25. Basic feedback loops for a fungal culture for antibiotic production <br />
  26. 26. Basic feedback loops for microbial high cell density formation<br />
  27. 27. Bioreactor Input and Output for basic PID control feed<br />
  28. 28.
  29. 29.
  30. 30.
  31. 31.
  32. 32.
  33. 33. Connections to USB, controller software and Ethernet for SCADA<br />
  34. 34. Supervisory Control and Data Acquisition syste (SCADA)<br />
  35. 35. Thank You<br />

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