Used for the treatment of </li></ul> Rheumatoid Arthritis , cancers <br /><ul><li>Antibody produced by a single clone of cells</li></li></ul><li>CLASSIFICATION OF BIOPHARMACEUTICALS :<br /><ul><li>VACCINE :
Biological preparation that</li></ul> improves immunity to<br /> a particular disease <br /><ul><li>They can be prophylactic or</li></ul> therapeutic<br /><ul><li>e.g. HBV vaccine
It is a Subunit Vaccine composed of only surface proteins, produced in the yeast</li></li></ul><li>CLASSIFICATION OF BIOPHARMACEUTICALS :<br /><ul><li>THROMBOLYTIC AGENTS :
Cell signalling proteins produced by the cells</li></ul> of the immune system <br /> in response to the challenges such as viruses<br /> & cancer cells<br /><ul><li>e.g. IFN –a , IFN -b , IFN -g </li></li></ul><li>CLASSIFICATION OF BIOPHARMACEUTICALS :<br /><ul><li>BLOOD FACTORS :
Tissue infected by virus<br />Interferon molecules produced<br />It is released and diffused to the <br />surrounding cells<br />It binds to the receptor<br /> of the adjacent cells<br />Production of anti – viral protein <br />This protein blocks the<br /> viral replication<br />
COMMERCIAL PRODUCTION OF INTERFERON<br />WHY ???<br /><ul><li>Physiochemical characterization
Clinical Application </li></li></ul><li>COMMERCIAL PRODUCTION OF INTERFERON type II<br />Blood collected in acid citrate-dextrose solution <br />by venipuncture<br />Peripheral blood lymphocytes – isolated by FHGM <br /> Lymphocytes also obtained by Plasmapheresis<br /> Lymphocytes suspended to 106 cells per ml in RPMI medium<br />
SEA (Staphylococcal Enterotoxin A) interferon inducer added (0.1 μl per ml)<br />Mercaptoethanol added.<br />Fetal calf serum added (10 %)<br />Roller bottles were seeded with 300 ml cell suspension<br />Roller bottles – gassed (7 % O2, 10% CO2, 83 % N – 1 min)<br />
Suspension was rotated (8 rpm – 37 ⁰C , 4 days) <br />Centrifuged (2000 rpm , 4 ⁰C) and supernatant collected<br />Controlled core-glass beads were added to interferon supernatant.<br />Glass beads containing INF was collected and centrifuged .<br />Beads were washed with phosphate buffered saline.<br />
Ethylene glycol added to elute interferon<br />Removal of SEA inducer by Immunoabsorption<br />Gel filtration was done on ultrogel to isolate the specific interferon. Fractions (5 ml) collected <br />pH and temp. studies done to monitor heat stability and any residual antiviral activity.<br />
Easy commercial production</li></li></ul><li>COMMERCIAL APPLICATIONS OF BIOPHARMACEUTICALS<br /><ul><li>Production of different biopharmaceutical products :
Epogen and EpoREL - Commercially produced erythropoietin hormone </li></ul> which is used against Anemia<br /><ul><li>Monoclonal Antibodies - Rituximab , Ceutximab - used against various lymphomas
Biopharmaceuticals produced by rDNA technology - Recombinant Vaccines e.g. Recombivax HB (hepatitis B viruses)</li></li></ul><li> CONCLUSION<br /><ul><li>Biopharmaceutical drugs account for 8% of total pharmaceutical market sales, and are the fastest growing part of the market.
Biopharma exports account for over 74 % of overall export from India in health industry.
Thus they may turn out to have the most profound influence on the future practice of molecular medicine.</li></li></ul><li>REFERENCES<br />Book of India Brand Equity Foundation<br />Medical Pharmacology – K. D. Tripathy<br />Immunology – Kuby (5th edition)<br />Biopharmaceutical - Wikipedia, the free encyclopedia.mht<br />Large-scale production and physicochemical characterization of human immune interferon.htm<br />biopharmaceuticals - Google Images.mht<br />Bioscience 2015 Vision.mht<br />Wiley InterScienceOnlineBook Summary.mht<br />interferon - drug class, medical uses, medication side effects, and drug interactions by MedicineNet_com.mht<br />