The report describes an immunofluorescence analysis of mouse liver tissue to detect the presence of phospho-p38 (T180/Y182) antibody. A polyclonal rabbit anti-phospho-p38 antibody was used at a 1:200 dilution overnight at 4°C, followed by a Cy3-labeled secondary antibody at 1:300 for 50 minutes at room temperature. DAPI counterstaining was also performed. The tissue underwent processing including antigen retrieval before primary and secondary antibody incubations, mounting, and visualization under a fluorescence microscope.