IκBα (NF-kappa-B inhibitor alpha) is a cellular protein that inhibits the NF-κB transcription factor through masking nuclear localisation signals of NF-κB proteins and keeping them inactive in the cytoplasm. The Phospho-IκB-α (S32/S36) polyclonal antibody detects endogenous levels of IκB-α protein only when phosphorylated at S32/S36.

1. Result
“A signal was detected using Phospho-IkappaB-alpha (S32/S26). Recent reports place IkappaB-alpha (S32/S26) at
the axon initial segment (AIS). However, no enrichment at the AIS was detected.” - P. Brites , Instituto de Biologia
Molecular e Celular-IBMC.
Cell Line:
In vitro cultured primary hip-
pocampal neurons
Method of validation: Immunofluorescence
Primary Antibody:
STJ90311 Anti-Phospho-IκB-α
(S32/S36)
Secondary Antibody: Donkey anti-rabbit AF568
Dilution ratio: 1:250, 1:1000
Protocol
 Neurons were fixed with 2%PFA
 Cells were washed 3 times for 5 minutes with PBS
 Cells were permeabilised with 0.2% triton X100 for 5 minutes at RT
 Cells were washed 3 times for 5 minutes with PBS
 Blocking occured with 0.5% fish gelatin in PBS for 1 hour at 37ºC
 The primary antibody was incubated overnight at 4ºC and then diluted to 1:250 or 1:1000 in 0.5% fish gelatin in PBS
 Cells were washed 3 times for 5 minutes with PBS
 The secondary antibody was incubated at RT for 1 hour and then diluted to 1:1000 in 0.5% fish gelatin in PBS
 Cells were washed 3 times for 5 minutes with PBS
 Cells were mounted in fluoroshield with DAPI moulding medium
Antibody Customer Review:
STJ90311 Anti-Phospho-IκB-α (S32/S36)
Antibody Rating:
1:250 dilution
1:1000 dilution