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Seed Basics 
Botany and Biology 
Russell T. Nagata 
College of Tropical Agri. and Human Resources 
Komohana Research and Education Center 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics 
Hilo, Hawaii 
russelln@hawaii.edu
Monocots and Dicots 
 Major division in plant biology 
 Angiosperm 
 Difference in plant growth pattern 
 Difference in seed germination 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Monocots 
 one cotyledon in seed 
 include all grasses 
 parallel veins 
 bundled vascular strands scattered 
 flower petals number 1, 3 and 6 
 secondary growth absent 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Monocots 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Monocot Seed 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Dicots 
 two cotyledons in seed 
 major veins netted 
 flower petals number 4, 5 and many 
 secondary stem growth 
 vascular bundle in ring 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Dicot Characteristics 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Dicot Seed 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Dicot Seed 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Comparison 
 One cotyledon 
 Petals multiple of 3 
 No secondary stem 
growth 
 Parallel major veins 
 Vascular bundle 
scattered 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics 
 Two cotyledon 
 4 or 5 petals 
 Secondary stem 
growth 
 Net like major veins 
 Vascular bundle in 
ring
Seed Types 
 OP or Open Pollinated Variety 
 Hybrid Variety 
 Heirloom Variety 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Open Pollinated 
Variety 
 allowed to “randomly mate” 
 variation in plant types are normal 
 good for self saving seeds 
 genetic drift constant issue 
 seeds generally low cost 
 includes most heirloom varieties 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Heirloom Variety 
 normally 2 to 3 generations or 60 years 
of planting history 
 began in an person’s garden or small 
farm 
 has local or regional adaption 
 has one or more outstanding 
characteristics 
 possible to self save seeds 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Hybrid Variety 
 created by control crossing of two plants 
 anthers emasculated by hand or 
physiologically, or by incompatibility 
 plant vigor often associated with hybrids 
 difficult to create hybrid seeds in home 
garden 
 usually most expensive seeds 
 cannot self save seed 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Mating Preferences 
 Self Pollinators 
 Out Crossers 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Self Pollinators 
 Self Compatible 
 Floral Morphology 
 enclose reproductive structures 
 tubes 
 pollination prior to flower opening 
 flower positioned to favor self pollination 
 Tomato, Snap Beans, Lettuce 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Out Crossers 
 Wind Pollinated 
 light, loose pollen 
 dependent on wind direction 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Out Crossers 
 Wind Pollinated 
 Insect Pollinated 
 availability of pollinators 
 pollinator preference 
 greenhouse production conditional 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Out Crossers 
 Wind Pollinated 
 Insect Pollinated 
 Vertebrate Pollinated 
 birds, mammals 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Out Crossers 
 Wind Pollinated 
 Insect Pollinated 
 Vertebrate Pollinated 
 Self Incompatibility 
 Chemical Recognition 
 Pollen and seed compatibility 
 Eg. Cabbage, Onions, Radish 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Out Crossers 
 Wind Pollinated 
 Insect Pollinated 
 Vertebrate Pollinated 
 Self Incompatibility 
 Floral Biology 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Floral Biology 
 Monoecious 
 one plant with male and female flowers 
 Dioecious 
 separate male and female flower plants 
 Receptivity 
 pollen shed and stigma receptivity not 
synchronized 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Out Crossers 
 Wind Pollinated 
 Insect Pollinated 
 Vertebrate Pollinated 
 Self Incompatibility 
 Floral Structure Dependent 
 Hybrid Vigor 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Out Crossers 
 Wind Pollinated 
 Insect Pollinated 
 Vertebrate Pollinated 
 Self Incompatibility 
 Floral Structure Dependent 
 Hybrid Vigor 
 Inbreeding Depression Possible 
 reduced plant vigor 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Pollen Transfer 
 Wind 
 direction important in small plots 
 loose pollen grains 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Pollen Transfer 
 Wind 
 Insects and Animals 
 Sticky Pollen Grains 
 Packaged Pollen Grains 
 Bees and Wasps 
 Beetles 
 Flies 
 Butterflies and Moths 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Pollen Transfer 
 Wind 
 Insects and Animals 
 Humans 
 Lack of Natural Pollinators 
 New Character Combinations 
 Variety Purity 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Reproductive Cycles 
 Annuals 
 Biennial 
 Perennial 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Annuals 
 Seed to Seed in One Growing Season 
 Herbaceous 
 Bean, pea, corn, lettuce, cucurbits 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Biennials 
 Require Two Growing Seasons 
 seed to seed 
 Need Dormancy or Photoperiod 
 cabbage, beets, carrots, collards, kale 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Perennials 
 Grow Many Years 
 Many Seed Cycles 
 Many Are Woody Plants 
 coconut, avocado, etc. 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Strategies on Saving 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics 
Seeds 
 What is the best source 
 Genetic diversity 
 Seed purity and rouging 
 Selection criteria 
 Isolation distances
What to Save From? 
 Heirlooms 
 Self Pollinated Crops 
 Annuals 
 Open Pollinated Varieties 
 No Hybrids Unless… 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Maintaining Genetic 
Diversity 
 How Many Plants to Keep 
 Mating Biology 
 Genetic Drift 
 Available Space 
 More Plants or More Seeds 
 Seed Yield Ratio 
 Seed Quantity Needed 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Maintaining Genetic 
Diversity 
 How Many Plants to Keep 
 Plant Selection 
 Reason For Selection 
 Eliminated Undesirables 
 Move in New Direction 
 New Possibility 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Maintaining Genetic 
Diversity 
 How Many Plants to Keep 
 Plant Selection 
 Population Makeup 
 Population Uniformity 
 Narrow Genetic Base 
 Open Pollinated 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Maintaining Genetic 
Diversity 
 How Many Plants to Keep 
 Plant Selection 
 Population Makeup 
 Pollination Biology 
 mating behavior 
 pollen transfer 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Seed Purity 
 Dependent on Starting Material 
 What are your objectives? 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Seed Purity 
 Dependent on Starting Material 
 Selection and Rouging Procedures 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Seed Purity 
 Dependent on Starting Material 
 Selection and Rouging Procedures 
 Cleanliness 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Seed Purity 
 Dependent on Starting Material 
 Selection and Rouging Procedures 
 Cleanliness 
 Proper Labeling – Seed to Seed 
 Variety Name 
 Common or Scientific Name 
 Date 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Seed Purity 
 Dependent on Starting Material 
 Selection and Rouging Procedures 
 Cleanliness 
 Proper Labeling – Seed to Seed 
 Seed Saving Goals and Objectives 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Seed Purity 
 Dependent on Starting Material 
 Selection and Rouging Procedures 
 Cleanliness 
 Proper Labeling – Seed to Seed 
 Seed Saving Goals and Objectives 
 Pollen Flow Control 
 Start to Finish 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Isolation Distances 
 Crop Specific 
 Pollination Biology 
 Self Pollination 
 Cleistogamy 
 Wind Pollinated 
 Insect Pollinated 
 Animal Pollinated 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Isolation Distances 
 Crop Specific 
 Location Specific 
 In Geographic Space 
 In Time 
 Exclusion of Pollinators 
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics
Hawai’i Public Seed Initiative 
Seed Saving and Production Basics

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Seed Basics: Botany and Biology

  • 1. Seed Basics Botany and Biology Russell T. Nagata College of Tropical Agri. and Human Resources Komohana Research and Education Center Hawai’i Public Seed Initiative Seed Saving and Production Basics Hilo, Hawaii russelln@hawaii.edu
  • 2. Monocots and Dicots  Major division in plant biology  Angiosperm  Difference in plant growth pattern  Difference in seed germination Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 3. Monocots  one cotyledon in seed  include all grasses  parallel veins  bundled vascular strands scattered  flower petals number 1, 3 and 6  secondary growth absent Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 4. Monocots Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 5. Monocot Seed Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 6. Dicots  two cotyledons in seed  major veins netted  flower petals number 4, 5 and many  secondary stem growth  vascular bundle in ring Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 7. Dicot Characteristics Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 8. Dicot Seed Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 9. Dicot Seed Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 10. Comparison  One cotyledon  Petals multiple of 3  No secondary stem growth  Parallel major veins  Vascular bundle scattered Hawai’i Public Seed Initiative Seed Saving and Production Basics  Two cotyledon  4 or 5 petals  Secondary stem growth  Net like major veins  Vascular bundle in ring
  • 11. Seed Types  OP or Open Pollinated Variety  Hybrid Variety  Heirloom Variety Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 12. Open Pollinated Variety  allowed to “randomly mate”  variation in plant types are normal  good for self saving seeds  genetic drift constant issue  seeds generally low cost  includes most heirloom varieties Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 13. Heirloom Variety  normally 2 to 3 generations or 60 years of planting history  began in an person’s garden or small farm  has local or regional adaption  has one or more outstanding characteristics  possible to self save seeds Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 14. Hybrid Variety  created by control crossing of two plants  anthers emasculated by hand or physiologically, or by incompatibility  plant vigor often associated with hybrids  difficult to create hybrid seeds in home garden  usually most expensive seeds  cannot self save seed Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 15. Mating Preferences  Self Pollinators  Out Crossers Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 16. Self Pollinators  Self Compatible  Floral Morphology  enclose reproductive structures  tubes  pollination prior to flower opening  flower positioned to favor self pollination  Tomato, Snap Beans, Lettuce Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 17. Out Crossers  Wind Pollinated  light, loose pollen  dependent on wind direction Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 18. Out Crossers  Wind Pollinated  Insect Pollinated  availability of pollinators  pollinator preference  greenhouse production conditional Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 19. Out Crossers  Wind Pollinated  Insect Pollinated  Vertebrate Pollinated  birds, mammals Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 20. Out Crossers  Wind Pollinated  Insect Pollinated  Vertebrate Pollinated  Self Incompatibility  Chemical Recognition  Pollen and seed compatibility  Eg. Cabbage, Onions, Radish Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 21. Out Crossers  Wind Pollinated  Insect Pollinated  Vertebrate Pollinated  Self Incompatibility  Floral Biology Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 22. Floral Biology  Monoecious  one plant with male and female flowers  Dioecious  separate male and female flower plants  Receptivity  pollen shed and stigma receptivity not synchronized Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 23. Out Crossers  Wind Pollinated  Insect Pollinated  Vertebrate Pollinated  Self Incompatibility  Floral Structure Dependent  Hybrid Vigor Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 24. Out Crossers  Wind Pollinated  Insect Pollinated  Vertebrate Pollinated  Self Incompatibility  Floral Structure Dependent  Hybrid Vigor  Inbreeding Depression Possible  reduced plant vigor Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 25. Pollen Transfer  Wind  direction important in small plots  loose pollen grains Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 26. Pollen Transfer  Wind  Insects and Animals  Sticky Pollen Grains  Packaged Pollen Grains  Bees and Wasps  Beetles  Flies  Butterflies and Moths Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 27. Pollen Transfer  Wind  Insects and Animals  Humans  Lack of Natural Pollinators  New Character Combinations  Variety Purity Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 28. Reproductive Cycles  Annuals  Biennial  Perennial Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 29. Annuals  Seed to Seed in One Growing Season  Herbaceous  Bean, pea, corn, lettuce, cucurbits Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 30. Biennials  Require Two Growing Seasons  seed to seed  Need Dormancy or Photoperiod  cabbage, beets, carrots, collards, kale Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 31. Perennials  Grow Many Years  Many Seed Cycles  Many Are Woody Plants  coconut, avocado, etc. Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 32. Strategies on Saving Hawai’i Public Seed Initiative Seed Saving and Production Basics Seeds  What is the best source  Genetic diversity  Seed purity and rouging  Selection criteria  Isolation distances
  • 33. What to Save From?  Heirlooms  Self Pollinated Crops  Annuals  Open Pollinated Varieties  No Hybrids Unless… Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 34. Maintaining Genetic Diversity  How Many Plants to Keep  Mating Biology  Genetic Drift  Available Space  More Plants or More Seeds  Seed Yield Ratio  Seed Quantity Needed Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 35. Maintaining Genetic Diversity  How Many Plants to Keep  Plant Selection  Reason For Selection  Eliminated Undesirables  Move in New Direction  New Possibility Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 36. Maintaining Genetic Diversity  How Many Plants to Keep  Plant Selection  Population Makeup  Population Uniformity  Narrow Genetic Base  Open Pollinated Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 37. Maintaining Genetic Diversity  How Many Plants to Keep  Plant Selection  Population Makeup  Pollination Biology  mating behavior  pollen transfer Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 38. Seed Purity  Dependent on Starting Material  What are your objectives? Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 39. Seed Purity  Dependent on Starting Material  Selection and Rouging Procedures Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 40. Seed Purity  Dependent on Starting Material  Selection and Rouging Procedures  Cleanliness Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 41. Seed Purity  Dependent on Starting Material  Selection and Rouging Procedures  Cleanliness  Proper Labeling – Seed to Seed  Variety Name  Common or Scientific Name  Date Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 42. Seed Purity  Dependent on Starting Material  Selection and Rouging Procedures  Cleanliness  Proper Labeling – Seed to Seed  Seed Saving Goals and Objectives Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 43. Seed Purity  Dependent on Starting Material  Selection and Rouging Procedures  Cleanliness  Proper Labeling – Seed to Seed  Seed Saving Goals and Objectives  Pollen Flow Control  Start to Finish Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 44. Isolation Distances  Crop Specific  Pollination Biology  Self Pollination  Cleistogamy  Wind Pollinated  Insect Pollinated  Animal Pollinated Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 45. Isolation Distances  Crop Specific  Location Specific  In Geographic Space  In Time  Exclusion of Pollinators Hawai’i Public Seed Initiative Seed Saving and Production Basics
  • 46. Hawai’i Public Seed Initiative Seed Saving and Production Basics