Polyhydroxyalkanoates (PHAs) are biodegradable and environmentally friendly bioplastics accumulate as storage materials by various bacteria. These PHAs serve as energy and carbon reserve materials for the bacteria. PHAs can be completely degraded within a span of 12-14 months by microbial consortia into CO2 and water. Wood waste as a carbon source for the isolation and screening of polyhydroxyalkanoates (PHAs) accumulating bacteria was endeavored during our study. Bacterial strains collected from various environmental sources were subjected to culture studies for PHA synthesis. Pseudomonas lignicola was the single strain which was able to synthesize PHA using the carbon source derived from wood waste, when compared to other bacterial strains.
Dinesh Khiladkar gave a seminar on biopolymers to the SCOE Department of Biotechnology in Pune in 2016-17. The seminar covered an introduction to biopolymers, their need and applications. It discussed the production and extraction of biopolymers like polyhydroxybutyrate and future prospects in using alternative carbon sources for fermentation processes to produce biodegradable plastics. The seminar provided references for further information on biopolymer production using low-cost substrates.
Polyhydroxyalkanoates as an example of natural biodegredable polymers .
PHAs are biodegredable biopolyesters produced by a variety of gram negative and gram positive bacteria.
They have a variety of applications in the industrial and medical fields .
bioplastics by microorganisms Polyhydroxyalkanoates And PolyhydroxybutyratePramod Pal
This document discusses bioplastics, which are plastics derived from renewable biomass sources such as vegetable oils, cornstarch, and pea starch. It notes that bioplastics are designed to biodegrade and can break down in either aerobic or anaerobic environments depending on how they are manufactured. Common types of bioplastics include polylactic acid (PLA), polyhydroxyalkanoic acids (PHAs), and polyhydroxybutyrate-co-valerate (PHBVs). The document also discusses the synthesis and production of bioplastics like PHAs and PHB by microorganisms, as well as their applications in packaging, catering, gardening, medical products, and sanitary products
Expression of Biodegradable plastics in PlantsGodwin J
This document discusses expressing biodegradable plastics like polyhydroxybutyrate (PHB) in plants. PHB is normally produced by bacteria like Ralstonia eutropha from glucose. The document proposes transferring the PHB biosynthesis genes from R. eutropha into plant chloroplast genomes to produce PHB in plant plastids. Experiments generated transgenic Arabidopsis and tobacco plants accumulating up to 5% of their dry weight as PHB localized to plastids. Targeting PHB production to maize and rapeseed plastids was also achieved. This approach addresses producing biodegradable plastics from renewable plant resources.
The document summarizes the production of polyhydroxybutyrate (PHB) using Alcaligenes eutrophus. Key points:
1. PHB is produced intracellularly by Alcaligenes eutrophus through fermentation of glucose in a nutrient-limited fed-batch process.
2. The process involves cultivation, centrifugation to obtain concentrated biomass, blending with solvents to extract PHB, and spray drying to obtain the final product.
3. Under optimal conditions, the process can produce 48.5 kg of PHB per hour, or 8,246 kg per year from 133 batches.
Biodegradable polymers are derived from biological sources such as plants and microorganisms. They include natural polymers like starch, cellulose, and proteins as well as synthetic polymers like polylactic acid (PLA) and polyhydroxyalkanoates (PHAs) that are biodegradable. PLA is commonly used for packaging and is produced from corn via fermentation. PHAs can be produced by microorganisms and have applications in drug delivery and tissue engineering. While biodegradable polymers address issues with conventional plastics, their production and properties need further improvement for widespread adoption. Continued research aims to enhance production efficiency and material properties.
The document discusses biodegradable polymers and their classification. It covers the history of biodegradable polymers and defines biodegradation. Biodegradable polymers are classified into categories including those derived from biomass, microorganisms, biotechnology, and petrochemical products. The mechanisms of biodegradation and various types of biodegradable polymers like photolytic, peroxidisable, and hydro-biodegradable polymers are also explained. Agricultural applications of biodegradable mulch films are highlighted.
Biopolymers can be divided into three categories based on their origin and production:
1) Polymers directly extracted from biomass like starch and cellulose
2) Polymers produced from biobased monomers through chemical synthesis like polylactic acid
3) Polymers produced by microorganisms or genetically modified bacteria like polyhydroxyalkanoates
Common biopolymers include starch, polylactic acid, polyhydroxyalkanoates, and polycaprolactone. These materials have properties similar to conventional plastics but are biodegradable. Their gas barrier and thermal properties depend on material and humidity conditions. Biopolymers can be composted within weeks to months depending on
Dinesh Khiladkar gave a seminar on biopolymers to the SCOE Department of Biotechnology in Pune in 2016-17. The seminar covered an introduction to biopolymers, their need and applications. It discussed the production and extraction of biopolymers like polyhydroxybutyrate and future prospects in using alternative carbon sources for fermentation processes to produce biodegradable plastics. The seminar provided references for further information on biopolymer production using low-cost substrates.
Polyhydroxyalkanoates as an example of natural biodegredable polymers .
PHAs are biodegredable biopolyesters produced by a variety of gram negative and gram positive bacteria.
They have a variety of applications in the industrial and medical fields .
bioplastics by microorganisms Polyhydroxyalkanoates And PolyhydroxybutyratePramod Pal
This document discusses bioplastics, which are plastics derived from renewable biomass sources such as vegetable oils, cornstarch, and pea starch. It notes that bioplastics are designed to biodegrade and can break down in either aerobic or anaerobic environments depending on how they are manufactured. Common types of bioplastics include polylactic acid (PLA), polyhydroxyalkanoic acids (PHAs), and polyhydroxybutyrate-co-valerate (PHBVs). The document also discusses the synthesis and production of bioplastics like PHAs and PHB by microorganisms, as well as their applications in packaging, catering, gardening, medical products, and sanitary products
Expression of Biodegradable plastics in PlantsGodwin J
This document discusses expressing biodegradable plastics like polyhydroxybutyrate (PHB) in plants. PHB is normally produced by bacteria like Ralstonia eutropha from glucose. The document proposes transferring the PHB biosynthesis genes from R. eutropha into plant chloroplast genomes to produce PHB in plant plastids. Experiments generated transgenic Arabidopsis and tobacco plants accumulating up to 5% of their dry weight as PHB localized to plastids. Targeting PHB production to maize and rapeseed plastids was also achieved. This approach addresses producing biodegradable plastics from renewable plant resources.
The document summarizes the production of polyhydroxybutyrate (PHB) using Alcaligenes eutrophus. Key points:
1. PHB is produced intracellularly by Alcaligenes eutrophus through fermentation of glucose in a nutrient-limited fed-batch process.
2. The process involves cultivation, centrifugation to obtain concentrated biomass, blending with solvents to extract PHB, and spray drying to obtain the final product.
3. Under optimal conditions, the process can produce 48.5 kg of PHB per hour, or 8,246 kg per year from 133 batches.
Biodegradable polymers are derived from biological sources such as plants and microorganisms. They include natural polymers like starch, cellulose, and proteins as well as synthetic polymers like polylactic acid (PLA) and polyhydroxyalkanoates (PHAs) that are biodegradable. PLA is commonly used for packaging and is produced from corn via fermentation. PHAs can be produced by microorganisms and have applications in drug delivery and tissue engineering. While biodegradable polymers address issues with conventional plastics, their production and properties need further improvement for widespread adoption. Continued research aims to enhance production efficiency and material properties.
The document discusses biodegradable polymers and their classification. It covers the history of biodegradable polymers and defines biodegradation. Biodegradable polymers are classified into categories including those derived from biomass, microorganisms, biotechnology, and petrochemical products. The mechanisms of biodegradation and various types of biodegradable polymers like photolytic, peroxidisable, and hydro-biodegradable polymers are also explained. Agricultural applications of biodegradable mulch films are highlighted.
Biopolymers can be divided into three categories based on their origin and production:
1) Polymers directly extracted from biomass like starch and cellulose
2) Polymers produced from biobased monomers through chemical synthesis like polylactic acid
3) Polymers produced by microorganisms or genetically modified bacteria like polyhydroxyalkanoates
Common biopolymers include starch, polylactic acid, polyhydroxyalkanoates, and polycaprolactone. These materials have properties similar to conventional plastics but are biodegradable. Their gas barrier and thermal properties depend on material and humidity conditions. Biopolymers can be composted within weeks to months depending on
Recent Advances In BioPolymers And Its ApplicationsArjun K Gopi
Biopolymers are materials that are biodegradable, derived from renewable resources, or both. Common biopolymers include polylactic acid (PLA), polyhydroxyalkanoates (PHAs), and cellulose. Biopolymers are increasingly important due to their environmentally-friendly properties and potential to replace petroleum-based plastics. However, biopolymers currently only account for about 1% of the global plastic market. The use of nanomaterials to create bionanocomposites can help improve biopolymer properties and expand their applications in areas like packaging, textiles, agriculture, and biomedicine.
Compatibilization in bio-based and biodegradable polymer blendsjeff jose
Compatibilization in bio-based and biodegradable polymer blends, Types, properties and application of biopolymers, Physical blending, Miscibility, compatibility, starch/pla blend,Compatiblizers used for starch/PLA blends, Non-reactive compatibilization,Compatibilization strategies in poly(lactic acid)-based blends
applications of polymer blends,
This document describes a three-stage process for producing polyhydroxyalkanoates (PHAs) from sugar cane molasses using mixed microbial cultures. The first stage involves continuous acidogenic fermentation of sugar cane molasses in a CSTR reactor, where the effect of pH on organic acid production is evaluated. The second stage is selection of a PHA-accumulating culture using either acetate or fermented molasses. The third stage is batch PHA accumulation using the selected culture and fermented molasses. Strategies for culture selection and their effects on polymer composition and yield are investigated.
Bioplastics are organic, biomass-based alternatives to conventional petroleum-based plastics. There are several types of bioplastics including PHA, PHB, Biopol, and PLA. PHB is produced naturally by bacteria as a carbon storage material and can be synthesized through bacterial fermentation. It is biodegradable and has applications in packaging and medical devices.
This document discusses biopolymers, which are polymers derived from living organisms. It defines biopolymers and provides examples such as cellulose, starch, and proteins. The document then covers the classification of biopolymers such as starch-based, sugar-based, and cellulose-based polymers. It also discusses the production and applications of biopolymers in packaging, agriculture, automotive and medical sectors. Finally, it outlines the environmental benefits and impacts of biopolymers.
It's about synthesis of bioplastic. specifically about PHA and bioplastic synthesis from red algae. It was completed under guidance of Mr. Abdul Shafiullah, Lecturer SSC, Shimoga
Today the world is facing problem related to spread of plastic all around us which cause infection and pollution. PET {poly(ethylene terephthalate)} is extensively used throughout the world. PET is made from petroleum and is widely used in textile industries and plastic bottles. Most of the PET product simply end up by land filling and never enter the recycling process. About 56 million ton of PET was produce worldwide in 2013 alone. Currently the only PET products being recycled are bottles, but the amount of recycled account are just 37% of the total production volume of PET bottle i.e. 6.13 million tons. Currently the chemical method is being used to recycle PET waste, which is quite energy consuming process and shows only assimilation of PET waste. Various microorganisms have also been reported to assimilate PET waste. However, assimilation is not the final solution of this problem as it is only a partial degradation. Recently, a novel microorganism Ideonella sakaiensis strain 201-F6 has been identified which uses PET as an energy resource and is able to produce environment friendly bi products such as ethylene glycol and terephthalic acid. Scientists also discovered two enzymes (PETase and MHETase) produced by the strain 201-F6 which hydrolyze PET. Based on the property of PETase and MHETase it is now understood that the strain 201-F6 is capable to use PET as its major energy source and convert it into easily degradable components.
Biodegradation is the chemical dissolution of materials by bacteria or other biological means.
biodegradable simply means to be consumed by microorganisms and return to compounds found in nature
This document characterizes various properties of an exopolysaccharide (EPS) produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibetan kefir, including its molecular weight, solubility, water and oil binding capacities, structure as viewed by scanning electron microscopy and atomic force microscopy, rheological properties under different conditions, melting point, and degradation temperature. The molecular weight was determined to be 5.5 x 10^4 Da by gel permeation chromatography. Solubility in water was 14.2% and water and oil binding capacities were 496.0% and 884.74% respectively. Scanning electron microscopy showed a smooth surface structure and atomic force microscopy revealed an almost
Recently, the advantages of biopolymers over conventional plastic polymers are unprecedented, provided that they are used in situations in which they raise the functionality and generate extra benefits for human life. Therefore, biopolymers have received much attention because they play an important place in day-to-day life for their specific tunable characteristics, making them attractive in a wide range of applications. Biopolymers can produce materials with tunable properties such as biodegradability, biocompatibility, renewability, inexpensiveness, availability, which are critically important for designing materials for use in biomedical applications. In addition to these properties, smart biopolymers could be prepared by changing the polymer components, which would create more target oriented applications. Biopolymers are potentially used in biomedical applications, including drug delivery, infections, tissue engineering, wound healings, and other as wells.
This document discusses the production of microbial pigments for use in the food industry. It describes several microorganisms commonly used to produce pigments like beta-carotene, riboflavin, lycopene, and Monascus pigments. These pigments have applications as natural food colorants and additives. The document also outlines some benefits of microbial pigments like lower production costs and their potential health benefits.
This document presents a group project on the production of polyhydroxybutyrate (PHB) using Alcaligenes eutrophus. It contains the names and student IDs of the group members, and outlines the contents of their presentation, including a literature review on R. eutropha and PHB, usage and demand analysis of PHB, process description, material and energy balances, bioreactor design considerations, biomass separation via rotary filtration, and computational modeling. Diagrams of PHB inclusion bodies in R. eutropha and the proposed process flow diagram are also included.
The document discusses biodegradable polymers and their importance as an alternative to conventional plastics. It provides background on biodegradable polymers, describing how they are defined and how they differ from conventional plastics in being able to break down from the action of microorganisms. The document outlines the main types of biodegradable polymers, their applications in packaging, agriculture, and medical sectors, and how some automakers are starting to use biodegradable composites in vehicles.
The document discusses various types of bioplastics including biodegradable, compostable, and bio-based plastics. It describes key bioplastics like starch blends, cellulose, polylactic acid (PLA), polyhydroxyalkanoates (PHA), and their properties. PHAs can be produced through microbial fermentation and come in short-chain (SCL) or medium-chain (MCL) lengths. Commonly used PHAs include P(3HB) and P(3HB-co-3HV). Bioplastics offer advantages like reduced fossil fuel usage but also challenges like competing with food supply and requiring specialized facilities for decomposition. Future areas of development
- A bacterial strain was isolated from acidic soil that could produce polyhydroxyalkanoates (PHAs) under nitrogen-deficient conditions. The isolate was identified as Klebsiella pneumoniae through 16S rDNA sequencing.
- Optimization experiments found the isolate could produce 0.8 g/L of PHA with glucose as the carbon source and ammonium nitrate as the nitrogen source after 48 hours. PHA production was confirmed through UV spectrophotometry and FTIR analysis.
- Further optimization of carbon and nitrogen sources increased PHA production to 1.1 g/L with glucose and either ammonium chloride or peptone. The isolate was also moderately halotolerant, with optimal P
Biopolymers are polymers produced by living organisms and contain monomeric units that are covalently bonded to form larger structures. There are three main types of biopolymers: biorenewable, biodegradable, and biomaterials. Biopolymers include proteins, nucleic acids like DNA and RNA, and lipids. These biopolymers have various applications in areas like drug delivery, tissue engineering, and medical implants. Biodegradable polymers break down into natural byproducts and are used for things like drug delivery systems.
Isolation, Screening, and Characterization of Biosurfactant-Producing Microor...BRNSS Publication Hub
Introduction: Biosurfactants are amphiphatic in nature and are surface-active compounds produced by microorganisms. These molecules reduce interfacial surface tension between aqueous solutions and hydrocarbon mixtures. Unfortunately, oil spills and industrial discharges from petroleum-related industries have been identified as the major pollution sources. The hydrophobicity and low aqueous solubility of petroleum pollutant limit the biodegradation process. The features that make biosurfactants as an alternative to commercially synthesized surfactants are its low toxicity, higher biodegradability and, hence, greater environmental compatibility, better foaming properties, and stable activity at extreme pH, temperature, and salinity. Objective: Therefore, in this study, hydrocarbon-degrading bacteria were screened from petroleum-contaminated soil, characterized and optimization of the physical and nutrient parameters were done to enhance the production of biosurfactants. Results: Petroleum-contaminated soil was collected from different petrol pumps in Pune and screening was done on minimal salt medium media containing palm oil as carbon source using hemolytic activity, emulsification index, drop-collapse test, and oil displacement method. The most promising strain was isolated and identified using Bergey’s Manual of Determinative Biology and 16s rRNA sequencing and was found to be Staphylococcus epidermidis. The optimization of various parameters, namely temperature, pH, carbon, and nitrogen sources on growth, and biosurfactant production was studied. The highest biosurfactant production was obtained when MSS media contains sucrose (carbon source) and urea (nitrogen source) at pH 10 and temperature 55°C. The Fourier transform-infrared (FT-IR) analysis of purified biosurfactant indicated the presence of lipopeptide biosurfactant when compared with reference FT-IR spectra.
1. The study investigated producing bioethanol from neem tree leaves through enzymatic hydrolysis and fermentation.
2. Dried and powdered neem leaves were hydrolyzed using varying amounts of Bacillus suspension to break down the leaves into simple sugars. The hydrolyzed leaves were then fermented using Bacillus firmus to produce bioethanol.
3. The highest bioethanol yield of 1.85% was obtained using 1.5cm3 of Bacillus suspension for hydrolysis. FTIR analysis confirmed the presence of alcohol in the bioethanol samples.
This document summarizes the extraction and characterization of polyhydroxybutyrates (PHB) from Bacillus thuringiensis KSADL127 isolated from mangrove environments in Saudi Arabia. 50 bacterial strains from mangrove samples were screened for PHB production, with one strain producing 137 mg/L of PHB. This highest producing strain was identified as B. thuringiensis KSADL127 based on phenotypic and genetic analysis. PHB was extracted from the strain and characterized using various analytical techniques, confirming it was intracellularly accumulated PHB.
Recent Advances In BioPolymers And Its ApplicationsArjun K Gopi
Biopolymers are materials that are biodegradable, derived from renewable resources, or both. Common biopolymers include polylactic acid (PLA), polyhydroxyalkanoates (PHAs), and cellulose. Biopolymers are increasingly important due to their environmentally-friendly properties and potential to replace petroleum-based plastics. However, biopolymers currently only account for about 1% of the global plastic market. The use of nanomaterials to create bionanocomposites can help improve biopolymer properties and expand their applications in areas like packaging, textiles, agriculture, and biomedicine.
Compatibilization in bio-based and biodegradable polymer blendsjeff jose
Compatibilization in bio-based and biodegradable polymer blends, Types, properties and application of biopolymers, Physical blending, Miscibility, compatibility, starch/pla blend,Compatiblizers used for starch/PLA blends, Non-reactive compatibilization,Compatibilization strategies in poly(lactic acid)-based blends
applications of polymer blends,
This document describes a three-stage process for producing polyhydroxyalkanoates (PHAs) from sugar cane molasses using mixed microbial cultures. The first stage involves continuous acidogenic fermentation of sugar cane molasses in a CSTR reactor, where the effect of pH on organic acid production is evaluated. The second stage is selection of a PHA-accumulating culture using either acetate or fermented molasses. The third stage is batch PHA accumulation using the selected culture and fermented molasses. Strategies for culture selection and their effects on polymer composition and yield are investigated.
Bioplastics are organic, biomass-based alternatives to conventional petroleum-based plastics. There are several types of bioplastics including PHA, PHB, Biopol, and PLA. PHB is produced naturally by bacteria as a carbon storage material and can be synthesized through bacterial fermentation. It is biodegradable and has applications in packaging and medical devices.
This document discusses biopolymers, which are polymers derived from living organisms. It defines biopolymers and provides examples such as cellulose, starch, and proteins. The document then covers the classification of biopolymers such as starch-based, sugar-based, and cellulose-based polymers. It also discusses the production and applications of biopolymers in packaging, agriculture, automotive and medical sectors. Finally, it outlines the environmental benefits and impacts of biopolymers.
It's about synthesis of bioplastic. specifically about PHA and bioplastic synthesis from red algae. It was completed under guidance of Mr. Abdul Shafiullah, Lecturer SSC, Shimoga
Today the world is facing problem related to spread of plastic all around us which cause infection and pollution. PET {poly(ethylene terephthalate)} is extensively used throughout the world. PET is made from petroleum and is widely used in textile industries and plastic bottles. Most of the PET product simply end up by land filling and never enter the recycling process. About 56 million ton of PET was produce worldwide in 2013 alone. Currently the only PET products being recycled are bottles, but the amount of recycled account are just 37% of the total production volume of PET bottle i.e. 6.13 million tons. Currently the chemical method is being used to recycle PET waste, which is quite energy consuming process and shows only assimilation of PET waste. Various microorganisms have also been reported to assimilate PET waste. However, assimilation is not the final solution of this problem as it is only a partial degradation. Recently, a novel microorganism Ideonella sakaiensis strain 201-F6 has been identified which uses PET as an energy resource and is able to produce environment friendly bi products such as ethylene glycol and terephthalic acid. Scientists also discovered two enzymes (PETase and MHETase) produced by the strain 201-F6 which hydrolyze PET. Based on the property of PETase and MHETase it is now understood that the strain 201-F6 is capable to use PET as its major energy source and convert it into easily degradable components.
Biodegradation is the chemical dissolution of materials by bacteria or other biological means.
biodegradable simply means to be consumed by microorganisms and return to compounds found in nature
This document characterizes various properties of an exopolysaccharide (EPS) produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibetan kefir, including its molecular weight, solubility, water and oil binding capacities, structure as viewed by scanning electron microscopy and atomic force microscopy, rheological properties under different conditions, melting point, and degradation temperature. The molecular weight was determined to be 5.5 x 10^4 Da by gel permeation chromatography. Solubility in water was 14.2% and water and oil binding capacities were 496.0% and 884.74% respectively. Scanning electron microscopy showed a smooth surface structure and atomic force microscopy revealed an almost
Recently, the advantages of biopolymers over conventional plastic polymers are unprecedented, provided that they are used in situations in which they raise the functionality and generate extra benefits for human life. Therefore, biopolymers have received much attention because they play an important place in day-to-day life for their specific tunable characteristics, making them attractive in a wide range of applications. Biopolymers can produce materials with tunable properties such as biodegradability, biocompatibility, renewability, inexpensiveness, availability, which are critically important for designing materials for use in biomedical applications. In addition to these properties, smart biopolymers could be prepared by changing the polymer components, which would create more target oriented applications. Biopolymers are potentially used in biomedical applications, including drug delivery, infections, tissue engineering, wound healings, and other as wells.
This document discusses the production of microbial pigments for use in the food industry. It describes several microorganisms commonly used to produce pigments like beta-carotene, riboflavin, lycopene, and Monascus pigments. These pigments have applications as natural food colorants and additives. The document also outlines some benefits of microbial pigments like lower production costs and their potential health benefits.
This document presents a group project on the production of polyhydroxybutyrate (PHB) using Alcaligenes eutrophus. It contains the names and student IDs of the group members, and outlines the contents of their presentation, including a literature review on R. eutropha and PHB, usage and demand analysis of PHB, process description, material and energy balances, bioreactor design considerations, biomass separation via rotary filtration, and computational modeling. Diagrams of PHB inclusion bodies in R. eutropha and the proposed process flow diagram are also included.
The document discusses biodegradable polymers and their importance as an alternative to conventional plastics. It provides background on biodegradable polymers, describing how they are defined and how they differ from conventional plastics in being able to break down from the action of microorganisms. The document outlines the main types of biodegradable polymers, their applications in packaging, agriculture, and medical sectors, and how some automakers are starting to use biodegradable composites in vehicles.
The document discusses various types of bioplastics including biodegradable, compostable, and bio-based plastics. It describes key bioplastics like starch blends, cellulose, polylactic acid (PLA), polyhydroxyalkanoates (PHA), and their properties. PHAs can be produced through microbial fermentation and come in short-chain (SCL) or medium-chain (MCL) lengths. Commonly used PHAs include P(3HB) and P(3HB-co-3HV). Bioplastics offer advantages like reduced fossil fuel usage but also challenges like competing with food supply and requiring specialized facilities for decomposition. Future areas of development
- A bacterial strain was isolated from acidic soil that could produce polyhydroxyalkanoates (PHAs) under nitrogen-deficient conditions. The isolate was identified as Klebsiella pneumoniae through 16S rDNA sequencing.
- Optimization experiments found the isolate could produce 0.8 g/L of PHA with glucose as the carbon source and ammonium nitrate as the nitrogen source after 48 hours. PHA production was confirmed through UV spectrophotometry and FTIR analysis.
- Further optimization of carbon and nitrogen sources increased PHA production to 1.1 g/L with glucose and either ammonium chloride or peptone. The isolate was also moderately halotolerant, with optimal P
Biopolymers are polymers produced by living organisms and contain monomeric units that are covalently bonded to form larger structures. There are three main types of biopolymers: biorenewable, biodegradable, and biomaterials. Biopolymers include proteins, nucleic acids like DNA and RNA, and lipids. These biopolymers have various applications in areas like drug delivery, tissue engineering, and medical implants. Biodegradable polymers break down into natural byproducts and are used for things like drug delivery systems.
Isolation, Screening, and Characterization of Biosurfactant-Producing Microor...BRNSS Publication Hub
Introduction: Biosurfactants are amphiphatic in nature and are surface-active compounds produced by microorganisms. These molecules reduce interfacial surface tension between aqueous solutions and hydrocarbon mixtures. Unfortunately, oil spills and industrial discharges from petroleum-related industries have been identified as the major pollution sources. The hydrophobicity and low aqueous solubility of petroleum pollutant limit the biodegradation process. The features that make biosurfactants as an alternative to commercially synthesized surfactants are its low toxicity, higher biodegradability and, hence, greater environmental compatibility, better foaming properties, and stable activity at extreme pH, temperature, and salinity. Objective: Therefore, in this study, hydrocarbon-degrading bacteria were screened from petroleum-contaminated soil, characterized and optimization of the physical and nutrient parameters were done to enhance the production of biosurfactants. Results: Petroleum-contaminated soil was collected from different petrol pumps in Pune and screening was done on minimal salt medium media containing palm oil as carbon source using hemolytic activity, emulsification index, drop-collapse test, and oil displacement method. The most promising strain was isolated and identified using Bergey’s Manual of Determinative Biology and 16s rRNA sequencing and was found to be Staphylococcus epidermidis. The optimization of various parameters, namely temperature, pH, carbon, and nitrogen sources on growth, and biosurfactant production was studied. The highest biosurfactant production was obtained when MSS media contains sucrose (carbon source) and urea (nitrogen source) at pH 10 and temperature 55°C. The Fourier transform-infrared (FT-IR) analysis of purified biosurfactant indicated the presence of lipopeptide biosurfactant when compared with reference FT-IR spectra.
1. The study investigated producing bioethanol from neem tree leaves through enzymatic hydrolysis and fermentation.
2. Dried and powdered neem leaves were hydrolyzed using varying amounts of Bacillus suspension to break down the leaves into simple sugars. The hydrolyzed leaves were then fermented using Bacillus firmus to produce bioethanol.
3. The highest bioethanol yield of 1.85% was obtained using 1.5cm3 of Bacillus suspension for hydrolysis. FTIR analysis confirmed the presence of alcohol in the bioethanol samples.
This document summarizes the extraction and characterization of polyhydroxybutyrates (PHB) from Bacillus thuringiensis KSADL127 isolated from mangrove environments in Saudi Arabia. 50 bacterial strains from mangrove samples were screened for PHB production, with one strain producing 137 mg/L of PHB. This highest producing strain was identified as B. thuringiensis KSADL127 based on phenotypic and genetic analysis. PHB was extracted from the strain and characterized using various analytical techniques, confirming it was intracellularly accumulated PHB.
bioplastics and biotechnology for sustainable futureRAJESHKUMAR428748
1. The document discusses bioplastics, which are plastics derived from renewable biomass sources such as vegetable oils and starches. Common bioplastics include polylactic acid (PLA), poly-3-hydroxybutyrate (PHB), and polyhydroxyalkanoates (PHA).
2. PHB is produced by certain bacteria as a carbon and energy storage material during nutrient stress conditions. It is synthesized through three enzymes and accumulates intracellularly.
3. Bioplastics are designed to be biodegradable and to have lower environmental impacts than fossil fuel-based plastics. They can break down aerobically or anaerobically depending on how they are manufactured.
The document discusses biodegradable plastics and polyhydroxyalkanoates (PHAs). It notes that traditional plastics are not biodegradable and accumulate in landfills, causing environmental issues. PHAs are introduced as a potential replacement as they are naturally produced and biodegraded by microorganisms. The document provides details on the production of PHAs by bacteria, their properties, and their biodegradability, establishing PHAs as a sustainable and biodegradable alternative to conventional plastics.
Microbial Production and Applications of PigmentsIRJET Journal
This document summarizes microbial production and applications of pigments. It discusses that natural pigments like carotenoids, flavonoids and tetrapyrroles are produced by microbes. The production of pigments by microbes involves isolating pigment-producing microbes from environments like soil and water. The microbes are then grown in nutrient media and their pigments are characterized. Some examples of pigment-producing microbes mentioned are Chryseobacterium sp. and Chromobacterium violaceum. Microbial pigments find applications as colorants in food, pharmaceutical and other industries due to their natural origin and biodegradability.
PRODUCTION AND CHARACTERIZATION OF BIOSURFACTANTS PRODUCED BY Pseudomonas aer...UniversitasGadjahMada
The biosurfactants are used by several industrial sectors such as petroleum, agriculture, food production, chemistry, cosmetics, and pharmaceuticals. Because of their hydrophobic and hydrophilic moieties, they have potency to reduce surface tension, interfacial tension between water-hydrocarbon systems, and low micelle concentration. Their characteristics strongly depend on the producer strain as well as on the medium composition, such as carbon and nitrogen sources. This study was conducted to investigate the influence of different sources of carbon (n-hexadecane, glycerol and glucose) and nitrogen (urea, NH4Cl and NaNO3 ) for the production of biosurfactants by a new strain of Pseudomonas aeruginosa B031 isolated from a rhizosphere of Paraserianthes falcataria L. Nielsen, a hardwood plant species at a phytoremediation field. The biosurfactant characteristics of the strain were evaluated, particularly its surface-active properties and potential to remove hydrocarbon. Glycerol was found to be the optimum carbon source, with rhamnose concentration, emulsification index, and critical micelle concentration (CMC) of 718 mg/L, 37%, and 35 mN/m, respectively. Sodium nitrate (NaNO3 ) was observed as the optimum nitrogen source, with rhamnose concentration, emulsification index, and CMC of 290 mg/L, 30%, and 24 mN/m, respectively. These biosurfactants efficiently reduced surface tension of culture broth from 42 mN/m to 31 mN/m for the glycerol treatment and from 37 mN/m to 24 mN/m for the sodium nitrate treatment. The crude biosurfactants from the glycerol and sodium nitrate treatments also removed 87.5% and 84%, respectively, of crude oil from sand. These rates were higher than those of the chemical surfactants (SDS and Triton X-100). These findings indicate that the biosurfactants produced by the strain from both glycerol and NaNO3 treatments can efficiently decrease the interfacial tension of culture broth dilution and have a high emulsion index, thus hold promise in hydrocarbon bioremediation application.
Screening of Laccase Producing White Rot Fungus from Decayed Woodijtsrd
This document summarizes a study that screened white rot fungi from decayed wood samples for their ability to produce laccase enzymes. Six fungal isolates from decayed wood were found to produce laccase when screened using guaiacol, tannic acid, and ABTS substrates on potato dextrose agar plates. ABTS proved to be the most efficient substrate for laccase screening. The potential applications of laccase enzymes in industries like pulp and paper, textiles, bioremediation and more are discussed. One fungal isolate (SM3) showing high laccase production was selected for further characterization of its laccase enzyme activity.
This document discusses bioplastics as an alternative to traditional plastics derived from fossil fuels. It provides background on bioplastics and their production. Global production of bioplastics has increased significantly in recent years and is projected to continue growing. Bioplastics have various advantages over traditional plastics like being renewable, biodegradable, and having a lower environmental impact. Common types include starch-based, PLA, and PHA bioplastics. They are used in packaging, electronics, catering, gardening, medical products and more. The production process and carbon cycle of bioplastics is also outlined.
This document describes a study that aimed to select Spirulina strains with high CO2 fixation capabilities for large-scale cultivation. Nine Spirulina species were screened in column bioreactors with 10% CO2 addition. Strains 208 and 220 were selected and optimized in 4 m2 raceway ponds for pH, dissolved inorganic carbon, and phosphate levels. The optimized conditions were then used to cultivate the two strains on a large scale of 605 m2 raceway ponds aerated with purified CO2 from a coal plant, achieving daily biomass production of up to 18.7 and 13.2 g/m2.
This document discusses various materials used for food packaging, including plastics, bioplastics, glass, and metals. It examines factors to consider when selecting a packaging type, like cost, storage requirements, and recyclability. The document also analyzes specific materials like PET, polystyrene, and BPA plastics, noting their potential to leach chemicals into foods. While bioplastics offer renewable alternatives, they also have limitations regarding brittleness and higher costs. Overall, the best packaging depends on the food product and aims to both preserve and protect food while avoiding harmful chemical leaching.
Wastes are materials that have not yet been fully utilized, they are leftovers from production and consumption. However, waste is an expensive and sometimes unavoidable result of human activity. It includes plant materials, agricultural, household, industrial and municipal wastes and residues (Okonkwo et al ., 2006). Large amount of wastes are generated every year from the industrial processing of agricultural raw materials and individual homes. Most of these wastes are used as animal feed or burned as alternative for elimination (Smith et al., 2009).
However, such wastes usually have a composition rich in sugars, minerals and proteins, and therefore, making them useful for other processes directly or indirectly (Pelczar et al., 2009). The presence of carbon sources, nutrients and moisture in these wastes provides conditions suitable for the development of microorganisms and this open up great possibilities for their reuse (Ofuya and Nwajuiba, 2004). The economical aspect is based on the fact that such wastes may be used as low-cost raw materials for the production of other value-added compounds, with the expectancy of reducing production costs (Smith et al., 2009). The environmental concern is because most of the agro-industrial wastes contain phenolic compounds and/or other compounds of toxic potential; which may cause deterioration of the environment when the waste is discharged to the nature (Theisen, 2010).
Microbiological studies depend on the ability to cultivate and maintain microorganisms under laboratory conditions by providing suitable culture media that offer favorable environmental conditions (okonkwo et al., 2006). A nutrient material prepared for the growth of microorganisms in a laboratory is called culture media (Gao et al., 2007). Microorganisms can obtain energy directly from sunlight while carbon can be made available in organic forms such as carbohydrates, or inorganic forms such as carbon dioxide and water (Ruth et al., 2012). The need to develop alternative media to various culture media has become imperative as the conventional media used are either not readily available or relatively expensive in most developing countries like Nigeria and other developing countries of the world (Pelczar et al., 2009).
Fungi are a group of eukaryotic spore bearing microorganisms. They generally reproduce asexually and sexually.Some are agents of diseases in plant and animals (parasitic) while some are saprophytic and play a major role in nutrient recycling (Gomez-pazos et al., 2005). Saprophytic fungi tend to be responsible for most of the disintegration of organic materials, and some of them render food material toxic (Pelczar et al., 2009).
Generally fungi are grown on Potato dextrose agar (PDA), Sabouraud’s dextrose agar (SDA), Rose Bengal Agar (RBA) and Corn Meal agar (CMA) which are very expensive (Okonkwo et al., 2006). Basically, every fungus requires carbon, nitrogen and energy source to grow and survive (Ainsworth and Bisby, 2005
The document discusses using algae for wastewater treatment. It notes that algae can efficiently treat wastewater by removing nutrients, heavy metals, and pathogens. This allows the treated water to be safely used for irrigation, reducing freshwater usage and pollution of waterways. Algae treatment in oxidation ponds also produces algal biomass that can be converted to biofuels or other products. The document provides examples of how different algae species effectively remove various wastewater contaminants like nitrogen, phosphorus, and COD. Algae wastewater treatment is seen as a more cost-effective and environmentally friendly alternative to conventional wastewater treatment methods.
Study on Characterization of Various Biofilms Prepared by Starch Isolated fro...ijtsrd
In the present study, the rhizome of Maranta arundinacea L., Arrowroot, was selected for a rich source of starch for the preparation of biofilm. Firstly, some physicochemical properties of the selected sample were determined by AOAC method. Furthermore, the elemental analysis of the selected sample was carried out by Energy Dispersive X ray Fluorescence EDXRF spectroscopy. Moreover, antimicrobial activities of various solvent extracts were examined by Agar well diffusion method on six tested organisms. And then, the qualitative determination of starch tests such as Iodine test and Tannic acid test were done. In addition, starch from Arrowroot powder was isolated and confirmed by FT IR spectrum. Finally, starch biofilms were prepared by using isolated starch and various ratios of plasticizers PVA, PEG, and Sorbitol. The characterizations of seven kinds of prepared biofilms were measured. Aye Mon Thida Nyo | Arnt Win | Baby San Chit Su | Mar Pi Myint | Phyu Phyu Khaing "Study on Characterization of Various Biofilms Prepared by Starch Isolated from Maranta Arundinacea L." Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26588.pdfPaper URL: https://www.ijtsrd.com/chemistry/other/26588/study-on-characterization-of-various-biofilms-prepared-by-starch-isolated-from-maranta-arundinacea-l/aye-mon-thida-nyo
Isolation and Screening of Hydrogen Producing Bacterial Strain from Sugarcane...Editor IJCATR
The aim of this study is to isolate a highly competent bacterium with potent cellulose degrading capability and a better
hydrogen producer. Soil sample from sugarcane bagasse yard was isolated, serially diluted and plated on cellulose specific nutrient
agar plate. Four colonies have been isolated in which a single colony has potent cellulose degrading ability and the highest hydrogen
productivity of 275.13 mL H2 L-1. The newly isolated bacterium was morphologically and biochemically characterized. The
molecular characterization of the bacterium was carried out using 16S rDNA sequencing and the organism was identified as
Bacilllus subtilis AuChE413. Proteomic analysis such as MALDI-TOF was carried out to differentiate the isolated Bacillus subtilis
from Bacillus thuringiensis and Bacillus amyloliquefaciens. Phylogenetic tree was constructed to analyze the evolutionary
relationship among different genus and species with the newly isolated strain.
Isolation, Characterization and Bioactivities of Sambucus wightiana derived D...ijtsrd
The demand for naturally occurring bioactive molecules such as antioxidant, anticancer and antimicrobial properties is emerging globally. More and more natural products are being investigated to isolate and characterize the bioactive small molecules. Therefore, the current research work was undertaken to isolate and characterize bioactive compounds from leaf part of Sambucus wightiana. Various hyphenated spectral techniques such as Uv-Vis, NMR, FT-IR and MS were undertaken to investigate identity of isolated compound. The study results isolation of Dotriacontanoic acid, an important molecule that possesses fabulous medicinal properties like antioxidant, antibacterial and antioxidant. Mudasir A Mir | Insha Zahoor | Amrina Shafi"Isolation, Characterization and Bioactivities of Sambucus wightiana derived Dotriacontanoic acid" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-6 , October 2017, URL: http://www.ijtsrd.com/papers/ijtsrd5812.pdf http://www.ijtsrd.com/biological-science/biotechnology/5812/isolation-characterization-and-bioactivities-of-sambucus-wightiana-derived-dotriacontanoic-acid/mudasir-a-mir
Biochar and its use in plant disease managementBISWAJITJENA64
This document discusses biochar and its potential use in plant disease management. It provides background on biochar production through pyrolysis and discusses several proposed mechanisms by which biochar may suppress plant diseases. These include inducing systemic resistance in plants, enhancing beneficial microbes, modifying soil quality, direct fungitoxic effects, and sorption of allelopathic compounds. The document reviews experimental evidence supporting these mechanisms from studies on various plant pathogens. It also summarizes research on biochar sorption of agrochemicals and phytotoxic compounds. Overall, the document examines the promising role of biochar as a tool for controlling plant diseases through multiple proposed mechanisms.
An alternative substrate for laccase production from Pleurotus spSaumya Dhup
This document summarizes a study that investigated using pine needles as an alternative substrate to wheat bran for laccase production through solid state fermentation using Pleurotus sp. Pine needles were found to produce higher laccase activity (311.27 U/ml) than wheat bran (258.75 U/ml). A fractional factorial design was used to optimize media supplements for laccase production. Relative humidity was also studied for its effect. The produced laccase was purified using aqueous two-phase separation. The objectives were to find cheaper alternatives to reduce production costs and optimize process parameters for laccase production using an alternative substrate.
This document provides an overview of research on treating vegetable waste. It characterizes the physical, chemical, and biological properties of various vegetable wastes. It then describes several treatment methods for vegetable waste, including anaerobic digestion to produce biogas, fluidized bed combustion, biodiesel production, and composting. The document also discusses identifying compounds in artichoke waste and comparing different waste treatment methods.
Biogas as a Alternate Source Of Energy And Creating Awareness Among Rural Pe...IJMER
This document discusses biogas as an alternative energy source and creating awareness among rural people. It provides 3 key points:
1) Biogas is a renewable gas produced from anaerobic digestion of organic waste like animal manure and plant materials. It is a cleaner substitute for firewood and fossil fuels.
2) There is a 3 step process for biogas production - hydrolysis, acidification, and methane formation - involving different bacteria that break down organic matter in the absence of oxygen.
3) Case studies describe floating drum and fixed dome biogas plants. Floating drum plants have a movable gas holder that rises as gas is produced while fixed dome plants have a non-movable gas holder above a fixed
1 ijfaf oct-2017-4-the characteristics of rumenAI Publications
The ruminant animal feed ingredients consisting of forage with the combination of grass and legume which were almost limited in availability, the cocoa leaves as agricultural wastes or wastes containing low nutrient plantation low used as alternative feed ingredients. To overcome these problems required a simple technology that is fermentation using microorganisms indigenus so that the effluent quality cocoa can be improved. Indigenus microorganisms were microbes that exist in individuals without disturbing the activities. The purpose of this research was to improve the quality of rumen fluid characteristics and digestibility of cocoa leaves fermented by microorganisms indigenus. The method used in this study was completely randomized design factorial with three replications, treatment factors consist of A as the level of microbes (A1 = 5%, A2 = 10%, A3 = 15%) and factor B as the duration of fermentation (B1 = 2 day, B2 = 4 days, B3 = 6 days) and the variables; the nutrient content and digestibility of cocoa leaves before and after being fermented and the characteristics of rumen fluid in vitro methods. The results showed that the increasing levels of use of microbes and fermentation time can enhance the quality of cocoa leaves that reduce the content of dry material from 31.389 to 25.488% and increase the protein content of the coarse 6.153 to 7.483%. Dry Material digestibility increased from 27.379 to 32.942% and 27.432% Protein 21,435- rough. Rumen fluid characteristics were rumen fluid pH ranges from 7.427 to 7.603, NH3 production ranges from 16.820 - 19,975mg / 100ml, and Volatyl Fatty Acid production from 83.667 to 101.000 mM. The conclusion that increasing the digestibility quality cocoa fruit leather could be made by fermentation by microorganisms indigenus.
Similar to Wood waste - carbon source for Polyhydroxyalkanoates (PHAs) production (20)
Evaluation of Agro-morphological Performances of Hybrid Varieties of Chili Pe...Premier Publishers
In Benin, chilli pepper is a widely consumed as vegetable whose production requires the use of performant varieties. This work assessed, at Parakou and Malanville, the performance of six F1 hybrids of chilli including five imported (Laali, Laser, Nandi, Kranti, Nandita) and one local (De cayenne), in completely randomized block design at four replications and 15 plants per elementary plot. Agro-morphological data were collected and submitted to analysis of variance and factor analysis of mixed data. The results showed the effects of variety, location and their interactions were highly significant for most of the growth, earliness and yield traits. Imported hybrid varieties showed the best performances compared to the local one. Multivariate analysis revealed that 'De cayenne' was earlier, short in size, thin-stemmed, red fruits and less yielding (≈ 1 t.ha-1). The imported hybrids LaaliF1 and KrantiF1 were of strong vegetative vigor, more yielding (> 6 t.ha-1) by developing larger, long and hard fruits. Other hybrids showed intermediate performances. This study highlighted the importance of imported hybrids in improving yield and preservation of chili fruits. However, stability and adaptation analyses to local conditions are necessary for their adoption.
An Empirical Approach for the Variation in Capital Market Price Changes Premier Publishers
The chances of an investor in the stock market depends mainly on some certain decisions in respect to equilibrium prices, which is the condition of a system competing favorably and effectively. This paper considered a stochastic model which was latter transformed to non-linear ordinary differential equation where stock volatility was used as a key parameter. The analytical solution was obtained which determined the equilibrium prices. A theorem was developed and proved to show that the proposed mathematical model follows a normal distribution since it has a symmetric property. Finally, graphical results were presented and the effects of the relevant parameters were discussed.
Influence of Nitrogen and Spacing on Growth and Yield of Chia (Salvia hispani...Premier Publishers
Chia is an emerging cash crop in Kenya and its production is inhibited by lack of agronomic management information. A field experiment was conducted in February-June and May-August 2021, to determine the influence of nitrogen and spacing on growth and yield of Chia. A randomized complete block design with a split plot arrangement was used with four nitrogen rates as the main plots (0, 40, 80, 120 kg N ha-1) and three spacing (30 cm x 15 cm (s1), 30 cm x 30 cm (s2), 50 cm x 50 cm (s3)). Application of 120 kg N ha-1 significantly increased (p≤0.05) vegetative growth and seed yield of Chia. Stem height, branches, stem diameter and leaves increased by 23-28%, 11-13%, 43-55% and 59-88% respectively. Spacing s3 significantly increased (p≤0.05) vegetative growth. An increase of 27-74%, 36-45% and 73-107% was recorded in number of leaves, stem diameter and dry weight, respectively. Chia yield per plant was significantly higher (p≤0.05) in s3. However, when expressed per unit area, s1 significantly produced higher yields. The study recommends 120 kg N ha-1 or higher nitrogen rates and a closer spacing of 15 cm x 30 cm as the best option for Chia production in Kenya.
Enhancing Social Capital During the Pandemic: A Case of the Rural Women in Bu...Premier Publishers
The document discusses a case study of enhancing social capital among rural women in Bukidnon Province, Philippines during the COVID-19 pandemic through a livelihood project. Key findings include:
1) Technical trainings provided by the project increased the women's knowledge, allowing them to generate additional household income through vegetable gardening during the pandemic.
2) The women's social capital, as measured by groups/networks, trust, and cooperation, increased by 15.5% from 2019 to 2020 through increased participation in their association.
3) Main occupations, income sources, and ethnicity influenced the women's social capital. The project enhanced social ties that empowered the rural women economically and socially despite challenges of the pandemic.
Impact of Provision of Litigation Supports through Forensic Investigations on...Premier Publishers
This paper presents an argument through the fraud triangle theory that the provision of litigation supports through forensic audits and investigations in relation to corporate fraud cases is adequate for effective prosecution of perpetrators as well as corporate fraud prevention. To support this argument, this study operationalized provision of litigation supports through forensic audit and investigations, data mining for trends and patterns, and fraud data collection and preparation. A sample of 500 respondents was drawn from the population of professional accountants and legal practitioners in Nigeria. Questionnaire was used as the instrument for data collection and this was mailed to the respective respondents. Resulting responses were analyzed using the OLS multiple regression techniques via the SPSS statistical software. The results reveal that the provision of litigation supports through forensic audits and investigations, fraud data mining for trends and patterns and fraud data collection and preparation for court proceedings have a positive and significant impact on corporate fraud prevention in Nigeria. This study therefore recommends that regulators should promote the provision of litigation supports through forensic audits and investigations in relation to corporate fraud cases in publicly listed firms in Nigeria, as this will help provide reports that are acceptable in court proceedings.
Improving the Efficiency of Ratio Estimators by Calibration WeightingsPremier Publishers
It is observed that the performances of most improved ratio estimators depend on some optimality conditions that need to be satisfied to guarantee better estimator. This paper develops a new approach to ratio estimation that produces a more efficient class of ratio estimators that do not depend on any optimality conditions for optimum performance using calibration weightings. The relative performances of the proposed calibration ratio estimators are compared with a corresponding global [Generalized Regression (GREG)] estimator. Results of analysis showed that the proposed calibration ratio estimators are substantially superior to the traditional GREG-estimator with relatively small bias, mean square error, average length of confidence interval and coverage probability. In general, the proposed calibration ratio estimators are more efficient than all existing estimators considered in the study.
Urban Liveability in the Context of Sustainable Development: A Perspective fr...Premier Publishers
Urbanization and quality of urban life are mutually related and however it varies geographically and regionally. With unprecedented growth of urban centres, challenge against urban development is more in terms of how to enhance quality of urban life and liveability. Making sense of and measuring urban liveability of urban places has become a crucial step in the context of sustainable development paradigm. Geographical regions depict variations in nature of urban development and consequently level of urban liveability. The coastal regain of West Bengal faces unusual challenges caused by increasing urbanization, uncontrolled growth, and expansion of economic activities like tourism and changing environmental quality. The present study offers a perspective on urban liveability of urban places located in coastal region comprising of Purba Medinipur and South 24 Parganas districts. The study uses the liveability standards covering four major pillars- institutional, social, economic and physical and their indicators. This leads to develop a City Liveability Index to rank urban places of the region, higher the index values better the urban liveability. The data for the purpose is collected from various secondary sources. Study finds that the eastern coastal region of the country covering state of West Bengal depicts variations in index of liveability determined by physical, economic, social and institutional indicators.
Transcript Level of Genes Involved in “Rebaudioside A” Biosynthesis Pathway u...Premier Publishers
Stevia rebaudiana Bertoni is a plant which has recently been used widely as a sweetener. This medicinal plant has some components such as diterpenoid glycosides called steviol glycosides [SGs]. Rebaudioside A is a diterpenoid steviol glycoside which is 300 times sweeter than table sugar. This study was done to investigate the effect of GA3 (50 mg/L) on the expression of 14 genes involved in Rebaudioside A biosynthesis pathway in Stevia rebaudiana under in vitro conditions. The expression of DXS remarkably decreased by day 3. Also, probably because of the negative feedback of GA3 on MEP-drived isoprenes, GGDS transcript level reached its lowest amount after GA3 treatment. The abundance of DXR, CMS, CMK, MCS, and CDPS transcripts showed a significant increase at various days after this treatment. A significant drop in the expression levels of KS and UGT85C2 is detected during the first day. However, expression changes of HDR and KD were not remarkable. Results revealed that the level of transcript of UGT74G1 and UGT76G1 up regulated significantly 4 and 2 times higher than control, respectively. However, more research needs to shed more light on the mechanism of GA3 on gene expression of MEP pathway.
Multivariate Analysis of Tea (Camellia sinensis (L.) O. Kuntze) Clones on Mor...Premier Publishers
Information on genetic variability for biochemical characters is a prerequisite for improvement of tea quality. Thirteen introduced tea clones characterized with objective; assessing tea clones based on morphological characters at Melko and Gera research stations. The study was conducted during 2017/18 cropping season on experimental plots in RCBD with three replications. Data recorded on morphological traits like days from pruning to harvest, height to first branch, stem diameter, leaf serration density, leaf length, leaf width, leaf size, petiole length, leaf ratio, internode length, shoot length, number of shoot, canopy diameter, hundred shoot weight, fresh leaf yield per tree. Cluster analysis of morphological trait grouped into four clusters indicated, the existence of divergence among the tested clones. The maximum inter-cluster distance was between clusters I and IV (35.27) while the minimum inter cluster distance was observed between clusters I and II (7.8).Principal components analysis showed that the first five principal components with eigenvalues greater than one accounted 86.45% for 15 morphological traits. Generally, the study indicated presence of variability for several morphological traits. However, high morphological variation between clones is not a guarantee for a high genetic variation; therefore, molecular studies need to be considered as complementary to biochemical studies.
Causes, Consequences and Remedies of Juvenile Delinquency in the Context of S...Premier Publishers
This research work was designed to examine nature of juvenile offences committed by juveniles, causes of juvenile delinquency, consequences of juvenile delinquency and remedies for juvenile delinquency in the context of Sub-Saharan Africa with specific reference to Eritrea. Left unchecked, juvenile delinquents on the streets engage in petty theft, take alcohol or drugs, rape women, rob people at night involve themselves in criminal gangs and threaten the public at night. To shed light on the problem of juvenile delinquency in the Sub-Saharan region data was collected through primary and secondary sources. A sample size of 70 juvenile delinquents was selected from among 112 juvenile delinquents in remand at the Asmara Juvenile Rehabilitation Center in the Eritrean capital. The study was carried out through coded self-administered questionnaires administered to a sample of 70 juvenile delinquents. The survey evidence indicates that the majority of the juvenile respondents come either from families constructed by unmarried couples or separated or divorced parents where largely the father is missing in the home or dead. The findings also indicate that children born out of wedlock, families led by single mothers, lack of fatherly role models, poor parental-child relationships and negative peer group influence as dominant causes of juvenile infractions. The implication is that broken and stressed families are highly likely to be the breeding grounds for juvenile delinquency. The survey evidence indicates that stealing, truancy or absenteeism from school, rowdy or unruly behavior at school, free-riding in public transportation, damaging the book of fellow students and beating other young persons are the most common forms of juvenile offenses. It is therefore, recommended that parents and guardians should exercise proper parental supervision and give adequate care to transmit positive societal values to children. In addition, the government, the police, prosecution and courts, non-government organizations, parents, teachers, religious leaders, education administrators and other stakeholders should develop a child justice system that strives to prevent children from entering deeper into the criminal justice process.
The Knowledge of and Attitude to and Beliefs about Causes and Treatments of M...Premier Publishers
Stigma and discrimination associated with mental illness are a common occurrence in the Sub-Saharan region including Eritrea. Numerous studies from Sub-Saharan Africa suggest that stigma and discrimination are major problems in the community, with negative attitudes and behavior towards people with mental illness being widespread. In order to assess the whether such negative attitudes persist in the context of Eritrea this study explored the knowledge and perceptions of 90 Eritrean university students at the College of Business and Economics, the University of Asmara regarding the causes and remedies of mental illness A qualitative method involving coded self-administered questionnaires administered to a sample of 90 university students to collecting data at the end of 2019. The survey evidence points that almost 50% of the respondents had contact with a mentally ill person suggesting that the significant number of the respondents experienced a first-hand encounter and knowledge of mental illness in their family and community. The findings show an overall greater science-based understanding of the causes of mental illness to be followed by recommended psychiatric treatments. The survey evidence indicates that the top three leading causes of mental illness in the context of Eritrea according to the respondents are brain disease (76%), bad events in the life of the mentally ill person (66%) and substance abuse or alcohol taking, smoking, taking drugs like hashish. (54%). The majority of the respondents have a very sympathetic and positive outlook towards mentally ill persons suggesting that mentally illness does not simply affect a chosen individual rather it can happen to anybody regardless of economic class, social status, ethnicity race and religion. Medical interventions cited by the majority of the respondents as being effective treatments for mental illness centered on the idea that hospitals and clinics for treatment and even cures for psychiatric disease. Changing perceptions of mental illnesses in Eritrea that paralleled the very caring and sympathetic attitudes of the sample university students would require raising public awareness regarding mental illness through education, using the mass media to raise public awareness, integrating mental health into the primary health care system, decentralizing mental health care services to increase access to treatment and providing affordable service to maintain positive treatment outcomes.
Effect of Phosphorus and Zinc on the Growth, Nodulation and Yield of Soybean ...Premier Publishers
This study investigated the effects of phosphorus and zinc on the growth, nodulation, and yield of two soybean varieties in Nigeria. Phosphorus application significantly affected growth, nodulation, yield, and some yield components, with 60 kg P2O5/ha giving the highest growth and yield. Phosphorus also increased nodulation, with 30 kg P2O5/ha providing the highest nodulation. Zinc application did not significantly affect most growth characters or nodulation, except for reducing plant height. Phosphorus increased soybean yield significantly to 1.9 t/ha compared to the control of 1.7 t/ha. Protein and oil contents were not significantly affected by phosphorus but were by zinc
Influence of Harvest Stage on Yield and Yield Components of Orange Fleshed Sw...Premier Publishers
A field experiment was conducted at Adami Tullu Agricultural Research Center in 2018 under rainfed condition with supplementary irrigation to determine the influence of harvest stage on vine yield and tuberous root yield of orange fleshed sweet potato varieties. The experiment consisted of four harvest stages (105, 120, 135 and 150 days after planting) and Kulfo, Tulla and Guntute varieties. A 4 X 3 factorial experiment arranged in randomized complete block design with three replications was used. Interaction of harvest stage and variety significantly influenced above ground fresh biomass, vine length, marketable tuberous root weight per hectare, commercial harvest index and harvest index. The highest mean values of above ground fresh biomass (66.12 t/ha) and marketable tuberous root weight (56.39 t/ha) were produced by Guntute variety harvested at 135 days after planting. Based on the results, it can be recommended that, farmers of the study area can grow Guntute variety by harvesting at 135 days after planting to obtain optimum vine and tuberous root yields.
Performance evaluation of upland rice (Oryza sativa L.) and variability study...Premier Publishers
This study evaluated 13 upland rice varieties over two locations in Ethiopia for yield and other traits. Significant differences were found among varieties for several traits. The highest yielding varieties were Chewaka, Hiddassie, and Fogera 1. Chewaka yielded 5395.8 kg/ha on average, 25.8-35% more than the check. Most varieties matured within 120-130 days. High heritability was found for days to heading, panicle length, and grain yield, indicating these traits can be easily improved through selection. Grain yield also had high genetic variation and heritability with genetic advance, suggesting yield can be improved through selection. This study identified variability that can be used
Response of Hot Pepper (Capsicum Annuum L.) to Deficit Irrigation in Bennatse...Premier Publishers
This study was conducted at Enchete kebele in Benna-Tsemay Woreda, South Omo Zone to evaluate the response of hot pepper to deficit irrigation on yield and water productivity under furrow irrigation system. The experiment comprised four treatments (100 % of ETc, 85% of ETc, 70 % of ETc and 50% of ETc), respectively. The experiment was laid out in RCBD and replicated four times. The two years combined yield results indicated that, the maximum total yield (20.38 t/ha) was obtained from 100% ETc while minimum yield (12.92 t/ha) was obtained from 50% of ETc deficit irrigation level. The highest WUE 5.22 kg/ha mm-1 was obtained from 50% of ETc. Treatment of 100% ETc irrigation application had highest benefit cost ratio (4.5) than all others treatments. Applying 50% of ETc reduce the yield by 37% when compared to 100 % ETc. Accordingly, to achieve maximum hot pepper yield in areas where water is not scarce, applying 100% ETc irrigation water application level throughout whole growing season under furrow irrigation system is recommended. But, in the study area water scarcity is the major limiting factor for crop production. So, it is possible to get better yield and water productivity of hot pepper when we apply 85% ETc irrigation water throughout growing season under furrow irrigation system.
Harnessing the Power of Agricultural Waste: A Study of Sabo Market, Ikorodu, ...Premier Publishers
Nigeria is still burdened with huge responsibilities of waste disposal because the potential for benefits of proper waste management is yet to be harnessed. The paper evaluates the capacity of the Sabo Cattle market in producing the required quantities of waste from animal dung alongside decomposed fruits with a view to generating renewable energy possibilities for lighting, security and other business activities of the market. It is estimated that about 998 million tons of agricultural waste is produced yearly in the country with organic wastes amounting to 80 percent of the total solid wastes. This can be categorized into biodegradable and non-biodegradable wastes. The paper evaluates the capacity of the Sabo Cattle market in producing the required quantities of waste from animal dung alongside decomposed fruits with a view to generating renewable energy possibilities for lighting, security and other business activities of the market. The Sabo market was treated as a study case with the adoption of in-depth examinations of the facility, animals and products for sale and waste generated. A combination of experimental, interviews (qualitative) and design simulation (for final phase) was adopted to extract, verify and analyse the data generated from the study. Animal waste samples were subjected to compositional and fibre analysis with results showing that the sample has high potency for biogas production. Biodegradable Wastes are human and animal excreta, agricultural and all degradable wastes. Availability of high quantity of waste generated being organic in Sabo market allows the use of anaerobic digestion to be proposed as a waste to energy technology due to its feasibility for conversion of moist biodegradable wastes into biogas. The study found that at peak supply period during the Islamic festivities, a conservative 300tonnes of animal waste is generated during the week which translates to over 800kilowatts of electricity.
Influence of Conferences and Job Rotation on Job Productivity of Library Staf...Premier Publishers
The general purpose of this study is to investigate the influence of conferences and job rotation on job productivity of library staff in tertiary institutions in Imo State, Nigeria. The survey research design was used for this study using questionnaire as an instrument for data collection. This study covered the entire population of 661. Out of these, 501 copies of the questionnaire representing 75.8% were duly completed and returned for analysis. Student’s t-test was used to analyze the research questions. The finding showed that conferences had no significant influence on the job productivity of library staff in tertiary institutions in Imo State, Nigeria (F cal= 7.86; t-vale =6.177; p >0.005). Finding also showed that job rotation significantly influences job productivity of library staff in tertiary institutions in Imo State, Nigeria (F-cal value= 18.65; t-value = 16.225; P<0.05). This study recommended that, government should ensure that library staff participate in conferences with themes and topics that are relevant to the job they perform and also ensure that there should be proper evaluation and feedback mechanism which aimed to ensuring control and minimize abuse of their development opportunities. Again, there should be written statement of objectives in order to sustain job rotation programmes. Also, that training and development needs of library staff must be identified and analyzed before embarking on job rotation processes as this would help to build skills, competences, specialization and high job productivity.
Scanning Electron Microscopic Structure and Composition of Urinary Calculi of...Premier Publishers
This document summarizes a study on the scanning electron microscopic structure and chemical composition of urinary calculi (stones) found in geriatric dogs. Microscopic examination of urine samples revealed increased numbers of blood cells, epithelial cells, pus cells, casts, bacteria and crystals of various shapes, predominantly struvite, calcium oxalate dihydrate and monohydrate, and ammonium urate. Scanning electron microscopy showed perpendicular columnar strata of struvite crystals and wavy phases of uric acid. Chemical analysis identified calcium phosphate, calcium oxalate and urea stones. The study characterized the microscopic and electron microscopic appearance of crystals and chemical composition of urinary calculi in geriatric dogs.
Gentrification and its Effects on Minority Communities – A Comparative Case S...Premier Publishers
This paper does a comparative analysis of four global cities and their minority districts which have been experiencing the same structural pressure of gentrification. The main contribution of this paper is providing a detailed comparison of four micro geographies worldwide and the impacts of gentrification on them: Barrio Logan in San Diego, Bo-Kaap in Cape Town, the Mission District in San Francisco, and the Rudolfsheim-Fünfhaus District in Vienna. All four cities have been experiencing the displacement of minority communities due to increases in property values. These cities were chosen because their governments enacted different policies to temper the gentrification process. It was found that cities which implemented social housing and cultural inclusionary policies were more successful in maintaining the cultural and demographic make-up of the districts.
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Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
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Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
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analyze the transformations that have taken place over the course of a decade.
The complex relationship between human activities and the environment has been the focus
of extensive research and worry. As the global community grapples with swift urbanization,
population expansion, and economic progress, the effects on natural ecosystems are becoming
more evident. A crucial element of this impact is the alteration of vegetation cover, which plays a
significant role in maintaining the ecological equilibrium of our planet.Land serves as the foundation for all human activities and provides the necessary materials for
these activities. As the most crucial natural resource, its utilization by humans results in different
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land.
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like India, rapid population growth and the emphasis on extensive resource exploitation can lead
to significant land degradation, adversely affecting the region's land cover.
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centuries, evolving its structure over time and space. In the present era, these changes have
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providing crucial environmental data for scientific, resource management, policy purposes, and
diverse human activities.
Accurate understanding of land use and cover is imperative for the development planning
of any area. Consequently, a wide range of professionals, including earth system scientists, land
and water managers, and urban planners, are interested in obtaining data on land use and cover
changes, conversion trends, and other related patterns. The spatial dimensions of land use and
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these patterns indicate shifts in economic and social conditions. Monitoring such changes with the
help of Advanced technologies like Remote Sensing and Geographic Information Systems is
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Remote Sensing and Geographic Information Systems
9
Changes in vegetation cover refer to variations in the distribution, composition, and overall
structure of plant communities across different temporal and spatial scales. These changes can
occur natural.
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2. Wood waste - carbon source for Polyhydroxyalkanoates (PHAs) production
Kumar M 037
fermentation and more economical recovery processes
(Godbole et al., 2003). High productivity is one of the major
factors for economical production of biodegradable
polymers (Chen et al., 2001). The carbon source should
be inexpensive since it is the major contributor to the total
substrate cost (Gao et al., 2002). Wood hydrolysate is a
potentially inexpensive and renewable feedstock that can
be produced through enzymatic or dilute acid hydrolysis of
cellulose or hemicellulose to fermentable sugars, such as
glucose, galactose, xylose, and mannose. Wood
hydrolysate has already been utilized for the production of
ethanol and xylitol. The isolation and development of
bacterial strains that can utilize such wood hydrolysates as
substrates is pursued intensively. In this regard the
present research work was focused on isolation and
screening of potential bacteria for synthesizing
polyhydroxyalkanoates (PHAs) using cheap carbon
source.
MATERIAL AND METHODS
Isolation of bacteria: Soil industrial effluent samples was
taken from Bommasandra Industrial Area (Paper & pulp) –
BIA and Kadugondana Halli (Tannery) – KH, Bangalore,
Karnataka, India were used for isolation of the bacteria.
Around 1.0 g of sample was serially diluted in sterile
distilled water and plated onto nutrient agar plates and
incubated at 30˚C for 24 hours. Various colonies of
different morphologies were individually picked and were
screened for PHA production, potential isolates were sub
cultured on nutrient agar plates as reported by Aarthi and
Ramana, 2011. The original cultures were maintained as
glycerol stock at –20°C for further use.
Rapid screening of native bacterial isolates for PHB
production: All the bacterial isolates were qualitatively
tested for PHB production by Nile blue (as a fluorescent)
staining method (Ostle and Holt, 1982). For rapid
screening of PHB producers, bacterial isolates were
spread into nutrient agar plate and the plates were
incubated at 30°C for 24 hours. Acetone solution of Nile
blue (0.5µg/ml) was spread over the colonies and the
plates kept undisturbed for 15 minutes.
Preparation of wood hydrolysate: Wood extracts
processing was carried out, for wood chips (at 160°C for
120 min), and hydrolysed with 2% sulphuric acid at 95°C
for 20 min., followed by concentration, neutralization, and
centrifugation for removal of precipitates (Chen et al.,
1984).
Analysis of wood hydrolysate for its contents: The
analysis of wood hydrolysate was performed with GC-MS
(JEOL GCMATE II GC-MS with Data system is a high
resolution, double focusing instrument, Maximum
resolution: 6000 Maximum calibrated mass: 1500 Daltons.
Source options: Electron impact {EI}, Chemical ionization
{CI}) for its knowing the actual sugar content of the source
material used for PHA bacteria.
Substrates for PHB production: Wood waste samples
was collected and dried in an oven at 60°C to reduce
moisture content and was milled into fine particles. The
following parameters such as reducing sugar, starch and
cellulose content in the raw seed were characterized (GC-
MS).
Bacterial Growth in specific media: Pure culture of
selected bacterial strain was revived in nutrient broth
initially and then grown in a defined Mineral Salt Media
(MSM) containing: 10g Hydrolyzed Seed, 5g Glucose, 5g
Sodium Chloride, 5g Di-Potassium Hydrogen Phosphate,
1g Potassium Chloride, 1g Magnesium Sulphate, and 1g
Ammonium Sulphate in 1L of distilled water. The pH of the
media was maintained to be 7.5±0.5. The culture flask was
kept in shaker at 150 rpm at 35 °C for two days (Amirul et
al., 2008, Du et al., 2001 and Yamanka et al., 2010).
Identification of PHA granules: The bacterial cells were
stained with Nile blue stain and visualized under UV
transilluminator (Geneflash, Syngene Bioimaging,
Cambridge, UK) and that gives a bright orange
fluorescence at a wavelength of 460nm. The accumulation
of PHA in the form of granules would be identified from the
fluorescing cells (Amirul et al., 2008).
RESULTS
Isolation of PHA producing bacteria
Polyhydroxyalkanoates (PHAs) are storage materials that
accumulate by various bacteria as energy and carbon
reserve materials. They are biodegradable,
environmentally friendly, and also biocompatible
bioplastics. Unlike petrochemical-based plastics that take
several decades to fully degrade, PHAs can be completely
degraded within a year by variety of microorganisms into
CO2 and water. Polyhydroxyalkanoic acids (PHAs) are
common intracellular compounds found in bacteria,
archaea, and in few eukaryotes such as yeasts and fungi.
PHAs are carbon and energy reserve polymers produced
in some microorganisms (e.g. Alcaligenes latus (FULAI
WANG AND SANG YUP LEE (1997)), Ralstonia eutropha
(Markus Potter, Mohamed H. Madkour, Frank Mayer and
Alexander Steinbuchel (2002)), Azotobacter beijerincki
(Soma PalA. MannaA.K. Paul (1999)), Bacillus
megaterium(GABRIEL J. MCCOOL AND MAURA C.
CANNON (1999)), when carbon source is in plentiful and
other nutrients such as nitrogen, phosphorus, oxygen or
sulfur are limited. Among the members of PHA family,
polyhydroxybutyrate (PHB) is the most common
biodegradable polymer and promising alternative to
synthetic non-degradable plastics. These polymers are
accumulated intracellular membrane enclosed inclusion
3. Wood waste - carbon source for Polyhydroxyalkanoates (PHAs) production
Int. J. For. Wood Sci. 038
Figure 1: (A) Isolate BIA 3 (refer Tab. No.1) from industrial effluents soil and (B) Isolate BIA 3 Figure 2: Culture plate of PHA producing
showing Fluorescence of PHA using Nile blue staining microorganism using Nile blue staining
Table 1: Characterization & Screening of PHA producing bacteria
S. No. Designation of Isolate Gram Reaction Shape
Plate Accumulation
Plate Count Nile Blue Staining
1 BIA 1 Gram Negative Cocci +++ +++
2 BIA 2 Gram Positive Cocci +++ ++
3 BIA 3 Gram Negative Small rod ++++ ++++
4 BIA 4 Gram Positive Cocci +++ +++
5 BIA 5 Gram Positive Dispersed rod +++ +++
6 KH 1 Gram Negative Small rod +++ +++
7 KH 2 Gram Negative Small rod ++ ++
8 KH 3 Gram Negative Dispersed rod + ++
9 KH 4 Gram Positive Cocci +++ +++
10 KH 5 Gram Positive Small rod ++++ +++
up to 90% of the cell dry weight under conditions of nutrient
stress and act as energy reserve material. These bacteria
have been reported from various environments, but only a
few from paper pulp and tannery effluents. For the rapid
detection and isolation of PHB producing bacteria, 0.02%
alcoholic solution of Nile blue A staining viable colony
method was used. The isolation of PHA producing bacteria
was done from paper pulp and tannery effluent water
samples. Most of the isolates showed positive result with
Nile blue A staining (Fig. No. 1). Both gram-positive and
gram-negative bacteria showed PHA production, but
gram-negative bacteria dominated the waste material
microflora of paper pulp and tannery industry.
The Nile Blue Staining method as experimented by Ostle
and Holt, 1982, was adopted visualizing PHA producing
microorganisms. The Nile blue was dissolved in acetone,
and was added to the agar medium for viable colony
staining. PHA producing microorganisms were visualized
as bright yellowish orange colonies under digital
microscope (Fig. No. 1B and 2).
Several bacteria were isolated from paper pulp and
tannery effluents by serial dilution method (Kumari Bhuwal
et al., 2013). From this, ten isolates were selected for PHA
production as shown in Table no. 1. Based on the intensity
of the fluorescence were observed in the Nile blue staining
method (Ostle and Holt, 1982), one potential PHA
producer (BIA 3) was screened out of ten isolates. The
granules showed Orange fluorescence under UV trans-
illuminator at a wavelength of 460nm as shown in Figures
1 - 2 and the results obtained was similar to Cortes et al.,
2008.
Characterization of wood hydrolysate
Hemicellulosic wood hydrolysate was analyzed by HPLC
and GC-MS to identify major carbon sources and inhibitory
compounds. The chromatographic profile of membrane
treated wood hydrolysate obtained by GC-MS is shown in
Fig. 3. Most of the pertinent peaks eluted within a retention
time of 20–30 min. Based on standards, the most
abundant peaks in the chromatogram were sugars,
including xylose, rhamnose, mannose, glucose, and some
minor sugar derivatives. Among the sugars, xylose was
the most abundant monosaccharide component, and
accounted for more than 85% of the total sugar content in
the hemicellulosic hydrolysate, followed by mannose,
rhamnose, and glucose (Table No. 2).
Chromatographic profile of membrane treated wood
hydrolysate obtained by GC-MS. Most of the pertinent
peaks eluted within a retention time of 18–35 min. Based
on standards, the most abundant peaks in the
4. Wood waste - carbon source for Polyhydroxyalkanoates (PHAs) production
Kumar M 039
Table 2: Composition of wood hydrolysate before and after membrane filtration
Substrates Before Membrane Treatment After Membrane Treatment
Xylose 170.7 g/L 70.7 g/L
Furfural 0.59 g/L N.D.
HMF 0.15 g/L 0.005 g/L
Acetate 0.78 m/L 0.05 m/L
Total phenolics (g/L GAE) 0.66 g/L 0.60 g/L
GAE – Gallic Acid Equivalent, N.D – Not Detected Molecular Weight cut-offs of 200 and 130 DA
Figure 3: Chromatogram of membrane-treated wood hydrolysate
Figure 4: Detection of PHA(B) produced by Pseudomonas lignicola under light microscope. (A) Bacterial
culture under light back ground (B) PHA bacteria under fluorescence back ground
chromatogram were sugars, including xylose, glucose and
other pentoses. Among the sugars, xylose was the most
abundant monosaccharide component, and accounted for
more than 80% of the total sugar content in the wood
hydrolysate, followed by glucose and rhamnose.
Identification of PHA granules
The accumulation of PHA in the form of granules would be
identified from the fluorescing cells as shown in figure 4.
Similar kind of studies was done earlier by Amirul et al.,
(2008) using fluorescence microscopy to visualize regions
of intracellular PHA accumulation.
DISCUSSION AND CONCLUSION
The isolation of PHA producing bacteria was done from
paper pulp and tannery effluent water samples. A large
proportion (%) of isolated bacteria produced PHA as
energy reserve material, proved by positive results with
Nile blue A staining. Both gram-positive and gram-
negative bacteria showed PHA production, but gram-
negative bacteria dominated the waste material microflora
of paper pulp and tannery industry. Hemicellulosic wood
hydrolysate was analyzed by HPLC and GC-MS to identify
major carbon sources and inhibitory compounds. The
chromatographic profile of membrane treated wood