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Wolbachia – The biology of
cytoplasmic incompatibility
By,
B.C. Muthubharathi,
III B.Sc.,Biotechnology.
What is Wolbachia?
Systematic classification
Kingdom : Bacteria
Phylum : Proteobacteria
Class : Alphaproteobacteria
Order : Rickettsiales
Family : Rickettsiaceae
Genus : Wolbachia
Species : melophagi
persica
pipientis
Infectsarthropod(insects), Nematodes
Parasitic microbe
1924 , Marshall hertig
Biocontrol agent
Biopesticide
Is it an Insecticide?
Intracellular
Infect reproductive tissue
Parthenogenesis
Feminization
Male Killing
Cytoplasmic Incompatibility
Alter the Phenotype
It affects
• Cherry Fruit Fly,
• Mosquitoes,
• Mites, Isopods etc.,
Cytoplasmic Incompatibility
• Results in sperm and egg unable to form viable
offspring
• Changes in gamete cells
• Leads to delayed entry of male pronucleus
• Embryonic mortality
Cellular Mechanism
28 Strains are identified
WMelPop-CLA : Reduce life span of adult
WMel : Induce reproductive phenotype
Modification - Spermatogenisis
Rescue - After fertilzation
Spematocytes and spermatides
Somatic cyst cell surrounding germcell
Prevent transmission of arbovirus , Human parasites
Protect infected insects from RNA virus
Role of Wolbachia in Reproduction of insects
Areas affected with Dengu
Cont..
Unidirectional CI: Mating of infected males and uninfected females results in
CI. All other crosses are compatible.
Bidirectional CI: Mating of males infected with Wolbachia strain I and females
infected by Wolbachia strain II (and vice versa) results in CI.
Wolbachia the biology of cytoplasmic incompatibility
Wolbachia the biology of cytoplasmic incompatibility
Wolbachia the biology of cytoplasmic incompatibility

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  • 1. Wolbachia – The biology of cytoplasmic incompatibility By, B.C. Muthubharathi, III B.Sc.,Biotechnology.
  • 2. What is Wolbachia? Systematic classification Kingdom : Bacteria Phylum : Proteobacteria Class : Alphaproteobacteria Order : Rickettsiales Family : Rickettsiaceae Genus : Wolbachia Species : melophagi persica pipientis Infectsarthropod(insects), Nematodes Parasitic microbe 1924 , Marshall hertig Biocontrol agent Biopesticide
  • 3. Is it an Insecticide? Intracellular Infect reproductive tissue Parthenogenesis Feminization Male Killing Cytoplasmic Incompatibility Alter the Phenotype It affects • Cherry Fruit Fly, • Mosquitoes, • Mites, Isopods etc.,
  • 4. Cytoplasmic Incompatibility • Results in sperm and egg unable to form viable offspring • Changes in gamete cells • Leads to delayed entry of male pronucleus • Embryonic mortality
  • 5. Cellular Mechanism 28 Strains are identified WMelPop-CLA : Reduce life span of adult WMel : Induce reproductive phenotype Modification - Spermatogenisis Rescue - After fertilzation Spematocytes and spermatides Somatic cyst cell surrounding germcell Prevent transmission of arbovirus , Human parasites Protect infected insects from RNA virus
  • 6. Role of Wolbachia in Reproduction of insects
  • 8. Cont.. Unidirectional CI: Mating of infected males and uninfected females results in CI. All other crosses are compatible. Bidirectional CI: Mating of males infected with Wolbachia strain I and females infected by Wolbachia strain II (and vice versa) results in CI.