The Paleozoic Era lasted from 542 to 251 million years ago, marked by the emergence of complex life forms and the largest mass extinction in Earth's history. This era witnessed significant biological diversity, beginning with the Cambrian explosion and culminating in the emergence of land-dwelling vertebrates and flight-capable insects. The era ended with the Permian extinction, where 96% of marine species and 70% of terrestrial species went extinct, possibly due to various catastrophic events.