I. The document discusses studies on the effects of oral administration of apolipoprotein A-I mimetic peptides synthesized from D-amino acids in reducing atherosclerosis in mice. It was found to dramatically decrease lesions by 75-79% independent of changes in total or HDL cholesterol.
II. The peptide is proposed to be useful for preventing and treating atherosclerosis and other inflammatory diseases caused by oxidized lipids. Its ability to reduce lesions orally suggests potential as a therapeutic agent.
III. Further studies are referenced that examine the mechanisms of action of apo A-I and analogs in protecting against atherosclerosis through reverse cholesterol transport and removal of oxidized lipids.
Plasma proteins, the components of plasma proteins, the protein fractions and condition causing the alteration in the each protein fraction. Clinical implications of the each fraction, the electrophorotic pattern of plasma protein. Acute phase proteins which include the positive and negative phase proteins.
Plasma proteins, the components of plasma proteins, the protein fractions and condition causing the alteration in the each protein fraction. Clinical implications of the each fraction, the electrophorotic pattern of plasma protein. Acute phase proteins which include the positive and negative phase proteins.
pharmacology of hypolipidemic drugs with outline of lipid metabolism ...... it helps in easy understanding ... i have focused solely on mechanism and key features of each class of drugs..... each class of drugs has specific use , i didnt mention it in my presentation ..... post ur comments in case u want to add , edit or want more info abt the topic ...
Basics of hyperlipoproreinemia in an easy and understandable way.gives a brief picture of the disease , it's cauusitive agents and clinical sequelae following it.
Cholesterol (from the Ancient Greek chole- (bile) and stereos (solid), followed by the chemical sufffix -ol for an alcohol) is an organic molecule. It is a sterol (or modified steroid), a type of lipid. Cholesterol is biosynthesized by all animal cells and is an essential structural component of animal cell membranes.
Cholesterol also serves as a precursor for the biosynthesis of steroid hormones, bile acid and vitamin D. Cholesterol is the principal sterol synthesized by all animals. In vertebrates, hepatic cells typically produce the greatest amounts. It is absent among prokaryotes (bacteria and archaea), although there are some exceptions, such as Mycoplasma, which require cholesterol for growth.
cholesterol is essential because it is involved in synthesis of
1. steroidal hormone
2.vitamin-d
3.bile acids
4. it is also structural component of cell membrane
IN PLASMA MEMBRANE
Cholesterol is important in the membrane as it helps to maintain cell membrane stability at varying temperatures. Cholesterol is bound to neighbouring phospholipid molecules via hydrogen bonds and therefore at low temperatures, reduces their packing.
2. TRANSPORTATION
Cholesteryl ester, a dietary lipid, is an ester of cholesterol. The ester bond is formed between the carboxylate group of a fatty acid and the hydroxyl group of cholesterol. Cholesteryl esters have a lower solubility in water due to their increased hydrophobicity. Esters are formed by replacing at least one –OH (hydroxyl) group with an –O–alkyl (alkoxy) group. They are hydrolyzed by pancreatic enzymes, cholesterol esterase, to produce cholesterol and free fatty acids
cholesterol is also important to maintain structure of lipoprotein
Cholesterol travels through the blood on proteins called “lipoproteins.” Two types of lipoproteins carry cholesterol throughout the body:
1.LDL (low-density lipoprotein), sometimes called “bad” cholesterol, makes up most of your body's cholesterol.
2.HDL (high-density lipoprotein) called “good cholesterol” makes a less part of your body’s cholesterol
Low beneficial effects of short term antidiabetic diet treatment in streptozo...iosrphr_editor
Oxidative stress is currently suggested to play a role in the pathogenesis of Diabetes mellitus. The role of dietary management in diabetes mellitus is to provide a proper balance of total nutrients while meeting the special dietary needs of the patient. The present study was designated to evaluate the effect of special antidiabetic diet treatment upon oxidative stress parameters in the initial stages of the development of diabetes. Male Wistar strain rats were used as an experimental model, divided into five groups. A significant decrease in superoxide dismutase and total glutathione activities were observed in the liver of diabetic rats when compared with control animals. The plasma level of aminotransferases, creatine kinase, lactate dehydrogenase and urea were significantly increased after induction of diabetes, in all groups under treatment. In contrast, rats fed special diet food, have shown slight different, but not significant changes. The findings of the present study suggest that special diet formula useful for prevention of progressive hyperglycaemia in age induced diabetes in dogs, could not restore the imbalance of cellular defence mechanism provoked by streptozotocin.
pharmacology of hypolipidemic drugs with outline of lipid metabolism ...... it helps in easy understanding ... i have focused solely on mechanism and key features of each class of drugs..... each class of drugs has specific use , i didnt mention it in my presentation ..... post ur comments in case u want to add , edit or want more info abt the topic ...
Basics of hyperlipoproreinemia in an easy and understandable way.gives a brief picture of the disease , it's cauusitive agents and clinical sequelae following it.
Cholesterol (from the Ancient Greek chole- (bile) and stereos (solid), followed by the chemical sufffix -ol for an alcohol) is an organic molecule. It is a sterol (or modified steroid), a type of lipid. Cholesterol is biosynthesized by all animal cells and is an essential structural component of animal cell membranes.
Cholesterol also serves as a precursor for the biosynthesis of steroid hormones, bile acid and vitamin D. Cholesterol is the principal sterol synthesized by all animals. In vertebrates, hepatic cells typically produce the greatest amounts. It is absent among prokaryotes (bacteria and archaea), although there are some exceptions, such as Mycoplasma, which require cholesterol for growth.
cholesterol is essential because it is involved in synthesis of
1. steroidal hormone
2.vitamin-d
3.bile acids
4. it is also structural component of cell membrane
IN PLASMA MEMBRANE
Cholesterol is important in the membrane as it helps to maintain cell membrane stability at varying temperatures. Cholesterol is bound to neighbouring phospholipid molecules via hydrogen bonds and therefore at low temperatures, reduces their packing.
2. TRANSPORTATION
Cholesteryl ester, a dietary lipid, is an ester of cholesterol. The ester bond is formed between the carboxylate group of a fatty acid and the hydroxyl group of cholesterol. Cholesteryl esters have a lower solubility in water due to their increased hydrophobicity. Esters are formed by replacing at least one –OH (hydroxyl) group with an –O–alkyl (alkoxy) group. They are hydrolyzed by pancreatic enzymes, cholesterol esterase, to produce cholesterol and free fatty acids
cholesterol is also important to maintain structure of lipoprotein
Cholesterol travels through the blood on proteins called “lipoproteins.” Two types of lipoproteins carry cholesterol throughout the body:
1.LDL (low-density lipoprotein), sometimes called “bad” cholesterol, makes up most of your body's cholesterol.
2.HDL (high-density lipoprotein) called “good cholesterol” makes a less part of your body’s cholesterol
Low beneficial effects of short term antidiabetic diet treatment in streptozo...iosrphr_editor
Oxidative stress is currently suggested to play a role in the pathogenesis of Diabetes mellitus. The role of dietary management in diabetes mellitus is to provide a proper balance of total nutrients while meeting the special dietary needs of the patient. The present study was designated to evaluate the effect of special antidiabetic diet treatment upon oxidative stress parameters in the initial stages of the development of diabetes. Male Wistar strain rats were used as an experimental model, divided into five groups. A significant decrease in superoxide dismutase and total glutathione activities were observed in the liver of diabetic rats when compared with control animals. The plasma level of aminotransferases, creatine kinase, lactate dehydrogenase and urea were significantly increased after induction of diabetes, in all groups under treatment. In contrast, rats fed special diet food, have shown slight different, but not significant changes. The findings of the present study suggest that special diet formula useful for prevention of progressive hyperglycaemia in age induced diabetes in dogs, could not restore the imbalance of cellular defence mechanism provoked by streptozotocin.
Whey protein products and their combination with L-methionine prevent liver f...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
A brief presentation about the current evidence based medical knowledge about the use of salt free albumin . After finishing this presentation you might discover that a lot of our practice lacks a solid basis regarding the use of this expensive drug.
Integrating Ayurveda into Parkinson’s Management: A Holistic ApproachAyurveda ForAll
Explore the benefits of combining Ayurveda with conventional Parkinson's treatments. Learn how a holistic approach can manage symptoms, enhance well-being, and balance body energies. Discover the steps to safely integrate Ayurvedic practices into your Parkinson’s care plan, including expert guidance on diet, herbal remedies, and lifestyle modifications.
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
1. Editorial Slides
VP Watch, January 23, 2002, Volume 2, Issue 3
Oral Apo A-I Mimetic Peptide for Reduction of
Atherosclerosis
2. Though there is variety of treatment methods for
atherosclerosis, there is clearly a need for an oral
agent inducing reduction of atherosclerosis.
Badimon, Fuster, and colleagues in 1989 showed
that administration of homologous HDL-VHDL
lipoprotein fraction to cholesterol-fed rabbits,
dramatically inhibited the extent of aortic fatty
streaks and lowered lipid deposition in the arterial
wall and liver without modification of the plasma
lipid levels.1
3. Shah et al. A single large dose of recombinant
apoA-I Milano rapidly increases the cholesterol
efflux–promoting capacity, mobilizes tissue
cholesterol, and reduces plaque lipid and
macrophage contents within 48 hours in apoE-
deficient mice. 6
Apo A-I Milano significantly reduced intimal
thickening and macrophage content after
balloon injury in cholesterol-fed rabbits without
a change in arterial total cholesterol content. 6
4. There are 2 mechanisms for apo A-I
( major apolipoprotein of HDL) which
protects against atherosclerosis in animals:
5,6
a. Reverse cholesterol transport,
b. Removal of low levels of oxidized lipids,
"seeding molecules" required to oxidize
LDL.
5. Infusion or transgenic expression of apo A-
I, the major apolipoprotein of HDL, protects
against atherosclerosis in animals. 2,3
Apo A-I and class A amphipathic helical
peptide analogs of apo A-I remove low
levels of oxidized lipids and prevent LDL
oxidation. 4,5
6. Eriksson et al. showed that after injection of
liposomes containing pro-apolipoprotein A-I,
fecal excretion of cholesterol increases. 7
Shah et al. demonstrated successful transfer
of apo A-I Milano gene into vascular smooth
muscle cells and bone marrow cells using
recombinant adeno-associated virus. 8
7. As highlighted in this week VP Watch, Navab
and colleagues found that only apolipoprotein A-I
mimetic peptide synthesized from D-amino acids
is stable in the circulation and enhanced the ability
of HDL to protect LDL against oxidation when
given orally to LDL receptor-null mice. 10
They discussed that mammalian enzymes such as
proteases recognize peptides and proteins
synthesized from L-amino acids but rarely
recognize those synthesized from D-amino
acids.10
8. They showed that when a peptide synthesized
from D-amino acids (D-4F) is administered orally
to LDL receptor-null mice on a Western diet,
lesions decrease by 79%. When added to the
drinking water of apoE-null mice, D-4F decreases
lesions by approximately 75%. 10
The plasma total cholesterol was not significantly
different for mice not receiving peptide and those
which received peptide. 10
9. At 4-weeks old, D-4F was added to the drinking water to give concentrations of
0.05 mg/mL (n=8 mice), 0.1 mg/mL (n=8 mice), 0.2 mg/mL (n=8 mice), 0.4
mg/mL (n=8 mice), 1.0 mg/mL (n=4 mice), or 2.0 mg/mL (n=4 mice), and no D-4F
was added to the drinking water of control mice (None) (n=13 mice). The mice
consumed approximately 2.5 mL of water per day. Values are mean±SEM.
*P<0.05 compared with control mice.
Reduction in plaque lesion area
Mohamad Navab, G.M. Anantharamaiah, Susan Hama, David W. Garber, Manjula Chaddha, Greg Hough,
Roger Lallone, and Alan M. Fogelman; Oral Administration of an Apo A-I Mimetic Peptide Synthesized From
D-Amino Acids Dramatically Reduces Atherosclerosis in Mice Independent of Plasma Cholesterol;
Circulation 2002 105: 290 - 292.
10. Navab and his colleagues recently reported
data on 27 patients with coronary
atherosclerosis who did not smoke, were not
diabetic, did not take hypolipidemic
medications, and whose total plasma
cholesterol, triglycerides, LDL, and HDL were
indistinguishable from 31 age- and sex-
matched normal controls. They showed that
27 coronary artery disease patients had
dysfunctional HDL similar to the LDL
receptor-null and apoE-null mice without D-
4F. 11
11. Conclusion:
I. Orally administered apo A-I mimetic peptides
synthesized from D-amino acids dramatically inhibit
atherosclerosis independent of changes in total
plasma or HDL-cholesterol.
II. This apo A-I mimetic peptides could be useful for the
prevention and treatment of atherosclerosis and other
chronic inflammatory illnesses that are caused by
oxidized lipids.
15. 1. Badimon JJ, Badimon L, Galvez A, Dische R, Fuster V.; High density lipoprotein
plasma fractions inhibit aortic fatty streaks in cholesterol-fed rabbits. Lab Invest.
1989 Mar;60(3):455-61.
2. Badimon JJ, Badimon L, Fuster V. Regression of atherosclerotic lesions by high
density lipoprotein plasma fraction in the cholesterol-fed rabbit. J Clin Invest.
1990; 85: 1234–1241.
3. Plump AS, Scott CJ, Breslow JL. Human apolipoprotein A-I gene expression
increases high density lipoprotein and suppresses atherosclerosis in the
apolipoprotein E-deficient mouse. Proc Natl Acad Sci U S A. 1994; 91: 9607–
9611.
4. Navab M, Hama SY, Cooke CJ, et al. Normal high density lipoprotein inhibits
three steps in the formation of mildly oxidized low density lipoprotein: step 1. J
Lipid Res. 2000; 41: 1481–1494.
5. Navab M, Hama SY, Anantharamaiah GM, et al. Normal high density lipoprotein
inhibits three steps in the formation of mildly oxidized low density lipoprotein:
steps 2 and 3. J Lipid Res. 2000; 41: 1495–1508.
6. Shah PK, Yano J, Reyes O, et al. High-dose recombinant apolipoprotein A-
Imilano mobilizes tissue cholesterol and rapidly reduces plaque lipid and
macrophage content in apolipoprotein E-deficient mice: potential implications for
acute plaque stabilization. Circulation. 2001; 103: 3047–3050.
7. Mohamad Navab, G.M. Anantharamaiah, Susan Hama, David W. Garber,
Manjula Chaddha, Greg Hough, Roger Lallone, and Alan M. Fogelman; Oral
References
16. 8. Eriksson M, Carlson LA, Miettinen TA, Angelin B.; Stimulation of fecal steroid
excretion after infusion of recombinant proapolipoprotein A-I. Potential reverse
cholesterol transport in humans. Circulation 1999 Aug 10;100(6):594-8
9. Shah PK, Kaul S, Nilsson J, Cercek B.; Exploiting the vascular protective effects
of high-density lipoprotein and its apolipoproteins: an idea whose time for testing
is coming, part II.Circulation. 2001 Nov 13;104(20):2498-502. Review.
10. Mohamad Navab, G.M. Anantharamaiah, Susan Hama, David W. Garber,
Manjula Chaddha, Greg Hough, Roger Lallone, and Alan M. Fogelman; Oral
Administration of an Apo A-I Mimetic Peptide Synthesized From D-Amino Acids
Dramatically Reduces Atherosclerosis in Mice Independent of Plasma
Cholesterol; Circulation 2002 105: 290 - 292.
11. Navab M, Hama SY, Hough GP, et al. A cell-free assay for detecting HDL that is
dysfunctional in preventing the formation of or inactivating oxidized
phospholipids. J Lipid Res. 2001; 42: 1308–1317.
References