1. The protein DPF3b recognizes acetylation marks on histones that are important for muscle growth and heart development. This discovery expands our understanding of epigenetic regulation of genes. 2. RNA polymerase plays a key role in transcription by unwinding DNA and forming a transcription bubble to access the DNA sequence. The process of transcription initiation occurs in three steps and is conserved across organisms. 3. Understanding the mechanisms of gene expression could lead to new treatments for diseases and predictive biomarkers by discovering novel regulatory proteins.

1. Transcription in prokaryotes and eukaryotes; gene Regulation. Lina Marcela Laverde PreciadoMedicine Third semester2010-2

2. FOLDING

3. FOLDING

4. INTRODUCTION

5. New waydiseases can develop: previouslyunknownmechanismdirects gene expression in cells

6. The DPF3b is a protein that recognizes a gene activator chemical marks on histones in the transcription is crucial for muscle growth and heart development

8. This discovery has broad implications in the field of epigenetic to human biology in health and diseaseScience of heritable changes that do not depend on the sequence of DNA bases

9. Brodomains are the protein domains that recognize acetylated lysine residues and are often essential for an association that may occur between proteins and histones and can rearrange the chromatin

10. OBSERVATION

11. ROLE OF RNA POLYMERASE IN GENE TRANSCRIPTION DEMONSTRATED

12. RNA polymerase plays an important role in the formation of the transcription bubble and in the process of bending the DNA to facilitate entry of DNA into the active site.

13. Sequence, structure and function of RNA polymerase is universally conserved from bacteria to humans, this facilitates the study of this.

14. The stage of transcription initiation of RNA polymerase consists of three stepsThe RNA polymerase binds with transcription promoting regions of DNAThrough interactions with the RNA polymerase, the DNA helix then unwindsForming an open "bubble" that allows the polymerase access to the exposed DNA sequence to begin transcription.

15. OBSERVATION

16. MEDICAL UTILITY

17. The expression of our genes is given by a combination of environmental factors, feeding radiation etc, when we come to understand the mechanism of expression can create new more effective treatments and also in terms of prevention

18. The discovery of new regulatory proteins may serve as biomarkers for certain diseases and acquire predictive value

19. BIBLIOGRAPHYLei Zeng, Qiang Zhang, SiDe Li, Alexander N. Plotnikov, Martin J. Walsh, Ming-Ming Zhou. Mechanism and regulation of acetylated histone binding by the tandem PHD finger of DPF3b. Nature, 2010; 466 (7303): 258 DOI: 10.1038/nature09139 available http://www.sciencedaily.com/releases/2010/07/100708152101.htmChen et al. From the Cover: Promoter melting triggered by bacterial RNA polymerase occurs in three steps. Proceedings of the National Academy of Sciences, 2010; 107 (28): 12523 DOI: 10.1073/pnas.1003533107 aviable http://www.sciencedaily.com/releases/2010/07/100715111559.htmMartínez LM. Transcripción genética, 5. ed. Medellín: Universidad Pontificia Bolivariana; 2009. p. 103-108.

20. Thanks…