This document discusses a study that used photomicrographs and computer modeling to better understand the role of RNA polymerase in DNA transcription. The study identified three key steps: 1) the DNA polymerase identifies promoter segments to transcribe, 2) it unwinds the DNA double helix to form a transcription bubble, and 3) the DNA refolds after transcription. Researchers were able to create a short movie of the transcription process starting, helping to illustrate this important step in genetic expression. Understanding transcription could help in developing new medical treatments by controlling gene expression or increasing it for therapeutic benefits.

1. Jhonny Alexander Castaño Morales ID 00144701 Universidad Pontificia Bolivariana

4. The transcription is very important process to expression the genetic material, use the RNA as molecule that transport the information since the nucleus to the cytoplasm, this process is controlled by different step that help that the genetic information don’t mutate

5. This new talk about the RNA polymerase and the important role of this molecule in the start and development of the transcription.

6. Explain as the RNA polymerase participate in the formation of the transcription bubble and the change of double helix to facility the union with the enzyme.

7. The investigation describe 3 steps to transcription de DNA, first the DNA polymerase indentify the promotor of the segment of DNA to transcribe, then the interaction to DNA polymerase and the segment of DNA unfold the double helix and make the transcription bubble and finally the DNA molecule is refold again.

8. This discovery was represented in computationally in a program that make the film of transcription the DNA and demonstrated the different roles of RNA polymerase in this process

9. I think that this investigation is very important for understand the performance of the mecanism that use the cell to express the genetic information, to expand the information and vision of this important process and develop new medical treatments based in this information

10. This article discusses about the importance of the DNA transcription to express the genetic information and how this process is controlled to prevent damage of many forms.

11. Researchers took many photomicrographs of the transcription process and then analyzed and created a small movie of the transcription start to represent better this step of the genetic material expression

12. different molecules were used to identify easily the process of transcription using different colors such as RAP1 (red), TFII (blue), loop of DNA (green)

13. the work of these researchers is very important because the analysis of the transcription process is easier with pictures and videos and so other researchers can discover more things based on this work, besides helping to teach

14. This research may be used to control the expression of genes that may cause harm to the patient and thereby controlling clinical manifestations or to increase the expression of some genes that can be used as a treatment for various diseases

15. This innovation is very important to clinical and laboratory application because the understand of the transcription process help to development of treatments for bacteries, for example.

16. With more information of transcription, the scientists develop more powerful and faster antibiotics that help the people to recuperate faster

17. - Martínez LM. Biología Molecular. 5. ed. Medellín: Universidad Pontificia Bolivariana; 2009. p. 101-108. - Science Daily, How DNA Is Copied Onto RNA Revealed Through Three-Dimensional Transcription Film - Science Daily Role of RNA Polymerase in Gene Transcription Demonstrated