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Chromatography is a technique used to separate and identify the components of a mixture. It works by allowing the molecules present in the mixture to distribute themselves between a stationary and a mobile medium. Thin layer chromatography (TLC) is a common form of chromatography that uses a thin layer of adsorbent like silica gel coated on a plate as the stationary phase. Samples are applied to the plate and then developed by moving the mobile phase up the plate, separating the sample components based on how strongly they adhere to the stationary phase. TLC is useful for identifying substances and testing purity through visualization of spots on the plate.





























