The cytoskeleton is a network of protein filaments that extends throughout the cytoplasm. It provides structure and organization to the cell, determining shape and positioning organelles. The three main types of filaments are actin filaments, intermediate filaments, and microtubules. Actin filaments are the thinnest filaments and form structures like filopodia, lamellipodia, and stress fibers. Microtubules are hollow cylinders composed of tubulin dimers and originate from the centrosome. They are involved in processes like cell division, organelle transport, and motility. Cilia and flagella project from the cell surface and use microtubule motors for movement.
A membrane protein is a protein molecule that is attached to, or associated with the membrane of a cell or an organelle.
More than half of all proteins interact with membranes.
Nucleus: Structure and function
nuclear membrane
nuclear lamins
Nuclear pore complexe
nuclear matrix, composition and its role
cajal bodies
SFCs
nuclear speckles
PML bodies
Nucleolus
A membrane protein is a protein molecule that is attached to, or associated with the membrane of a cell or an organelle.
More than half of all proteins interact with membranes.
Nucleus: Structure and function
nuclear membrane
nuclear lamins
Nuclear pore complexe
nuclear matrix, composition and its role
cajal bodies
SFCs
nuclear speckles
PML bodies
Nucleolus
Cytoskeleton - microtubules ,microfilaments and intermediate filamentsBIOTECH SIMPLIFIED
The cytoskeleton is a structure that helps cells maintain their shape and internal organization, and it also provides mechanical support that enables cells to carry out essential functions like division and movement. There is no single cytoskeletal component. Rather, several different components work together to form the cytoskeleton.
Details of cytoskeleton element-microtubule. The Microtubule associated protein-type and function, Treadmilling and dynamic instability, Structure of cilia and flagella
Structure and functions of endoplasmic reticulumICHHA PURAK
The presentation consists of 57 slides,describes following heads
• DISCOVERY
• INTRODUCTION
• BIOGENESIS OF ER
• ISOLATION OF MICROSOMES FROM E R
• STRUCTURE
• COMPONENTS OF ER
CISTERNAE
VESICLES
TUBULES
• MAIN FUNCTION OF ER
• TYPES OF ENDOPLASMIC RETICULUM
• SMOOTH ENDOPLASMIC RETICULUM (SER)
• FUNCTIONS OF SER
• ROUGH ENDOPLASMIC RETICULUM (RER)
• FUNCTIONS OF RER
• SUMMARY
• REFERENCES
• QUESTIONS
Structure and function of plasma membrane 2ICHHA PURAK
The presentation consists of 72 slides,describes following heads
DEFINITION : STRUCTURE OF PLASMA MEMBRANE
COMPONENTS OF PLASMA MEMBRANE ( (BIOCHEMICAL PROPERTIES)
LIPID BILAYER
PROTEINS
CARBOHYDRATES
CHOLESTEROL
MODELS EXPLAINING STRUCTURE OF BIO MEMBRANE
FLUID MOSAIC MODEL
MOBILITY OF MEMBRANE
GLYCOCALYX : GLYCOPROTEINS AND GLYCOLIPIDS
TRANSPORT OF IONS AND MOLECULES ACROSS PLASMA MEMBRANE
FUNCTIONS OF PLASMA MEMBRANE
DIVERSITY OF CELL MEMBRANES
SITE OF ATPASE ION CARRIER CHANNELS AND PUMPS-RECEPTORS
Cytoskeleton - microtubules ,microfilaments and intermediate filamentsBIOTECH SIMPLIFIED
The cytoskeleton is a structure that helps cells maintain their shape and internal organization, and it also provides mechanical support that enables cells to carry out essential functions like division and movement. There is no single cytoskeletal component. Rather, several different components work together to form the cytoskeleton.
Details of cytoskeleton element-microtubule. The Microtubule associated protein-type and function, Treadmilling and dynamic instability, Structure of cilia and flagella
Structure and functions of endoplasmic reticulumICHHA PURAK
The presentation consists of 57 slides,describes following heads
• DISCOVERY
• INTRODUCTION
• BIOGENESIS OF ER
• ISOLATION OF MICROSOMES FROM E R
• STRUCTURE
• COMPONENTS OF ER
CISTERNAE
VESICLES
TUBULES
• MAIN FUNCTION OF ER
• TYPES OF ENDOPLASMIC RETICULUM
• SMOOTH ENDOPLASMIC RETICULUM (SER)
• FUNCTIONS OF SER
• ROUGH ENDOPLASMIC RETICULUM (RER)
• FUNCTIONS OF RER
• SUMMARY
• REFERENCES
• QUESTIONS
Structure and function of plasma membrane 2ICHHA PURAK
The presentation consists of 72 slides,describes following heads
DEFINITION : STRUCTURE OF PLASMA MEMBRANE
COMPONENTS OF PLASMA MEMBRANE ( (BIOCHEMICAL PROPERTIES)
LIPID BILAYER
PROTEINS
CARBOHYDRATES
CHOLESTEROL
MODELS EXPLAINING STRUCTURE OF BIO MEMBRANE
FLUID MOSAIC MODEL
MOBILITY OF MEMBRANE
GLYCOCALYX : GLYCOPROTEINS AND GLYCOLIPIDS
TRANSPORT OF IONS AND MOLECULES ACROSS PLASMA MEMBRANE
FUNCTIONS OF PLASMA MEMBRANE
DIVERSITY OF CELL MEMBRANES
SITE OF ATPASE ION CARRIER CHANNELS AND PUMPS-RECEPTORS
Motor molecules also carry vesicles or organelles to various destinations along “monorails’ provided by the cytoskeleton.
Interactions of motor proteins and the cytoskeleton circulates materials within a cell via streaming.
Recently, evidence is accumulating that the cytoskeleton may transmit mechanical signals that re-arrange the nucleoli and other structures.
Describes about the major neurodegenerative disorders such as Dementia,Alzhimers disease,Parkinsons disease,Amyotrophic lateral sclerosis,etc.Their causes,symptoms and preventative measures.
Describes about the importance of vitamins in our daily activities , classification of vitamins,various sources of vitamins and also about the problems which occurs due to the deficiency of vitamins.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
3. • The cytoskeleton,is the network of protein filaments
extending throughout the cytoplasm of all eukaryotic
cells
• The cytoskeleton provides a structural framework for
the cell, that determines cell shape,the positions of
organelles, and the general organization of the
cytoplasm
• The cytoskeleton is composed of three principal types
of protein filaments such as Actin filaments,
Intermediate filaments, and Microtubules
4.
5. FUNCTIONS OF CYTOSKELETON
• Establishing cell shape
• Providing mechanical strength
• Locomotion
• Chromosome separation in mitosis and meiosis
• Intracellular transport of organelles
6. • The major cytoskeletal protein of most cells is Actin
• which polymerizes to form actin filaments- thin,
flexible fibers approximately 7 nm in diameter and
up to several micrometers in length
• Being the thinnest of the cytoskeletal filaments,This
are also called Microfilaments
8. • In skeletal muscle fibers they are called Thin
filaments
• Generate Cytoplasmic streaming in some cells
• Generate Locomotion in cells such as white blood
cells
• Interact with myosin ("thick") filaments in skeletal
muscle fibres to provide the force of muscular
contraction
10. • Filopodia (also called Microspikes) are long, thin and
transient structures that extend out from the cell surface.
• Bundles of parallel actin filaments, with their plus ends
oriented toward the filopodial tip
• Microvilli are shorter and more numerous protrusions of
the cell surface found in some cells.
• Tightly bundled actin filaments within these structures
their plus ends oriented toward the tip
• Small cross-linking proteins such as fimbrin and
villin bind actin filaments together within microvilli
provide stiffness
11.
12. Lamellipodia are thin but broad projections at the
edge of a mobile cell. Lamellipodia
are dynamic structures, constantly changing shape.
13. • Stress fibers form when a cell makes stable
connections to a substrate
• Bundles of actin filaments extend from the cell
surface through the cytosol. The actin filaments,
whose plus ends are oriented toward the cell
surface
• Myosin mediates sliding of anti-parallel actin
filaments during contraction of stress fibers
• -Actinin may cross-link actin filaments within
stress fibers
14.
15. • Some cells have a cytoskeletal network just inside the
plasma membrane that includes actin along with various
other proteins such as spectrin
• This cytoskeleton has a role in maintaining cell shape.
An example is found in erythrocytes
• Spectrin is an actin-binding protein that forms an
elongated tetrameric complex having an actin-binding
domain at each end
• With short actin filaments, spectrin forms a cytoskeletal
network on the cytosolic surface of the plasma
membrane of erythrocytes and some other cells
17. ORGANISATION OF ACTIN FILAMENTS
• Individual actin filaments are assembled into two general types
of structures called Actin bundles and Actin networks,
which play different roles in the cell.
• The first type of bundle, containing closely spaced actin
filaments aligned in parallel supports projections of the plasma
membrane, such as microvilli
• In these bundles, all the filaments have the same polarity, with
their barbed ends adjacent to the plasma membrane
• An example of a bundling protein involved in the formation of
these structures is fimbrin
• which was first isolated from intestinal microvilli and later
found in surface projections of a wide variety of cell types
18.
19. • The second type of actin bundle is composed of
filaments that are more widely spaced, allowing the
bundle to contract
• In contrast to fimbrin, actin binds to actin as a
dimer, each subunit of which is a 102 kd protein
containing a single actin-binding site
20. • The actin filaments in networks are held together by large actin-
binding proteins, such as filamin.
• Filamin binds actin as a dimer of two 280 kd subunits. The
actin-binding domains and dimerization domains are at opposite
ends of each subunit
• so the filamin dimer is a flexible V-shaped molecule with actin-
binding domains at the ends of each arm.
• As a result, filamin forms cross-links between orthogonal actin
filaments, creating a loose three-dimensional mesh .
21.
22. They have a diameter of about 25 nm
They are variable in length but can grow 1000 times as long as
they are wide
They are built by the assembly of dimers of alpha
tubulin and beta tubulin
They are straight, hollow cylinders whose wall is made up of a
ring of 13 "protofilaments”
They are found in both animal and plant cells.
In plant cells, microtubules are created at many sites scattered
through the cell.
In animal cells, the microtubules originate at the centrosome.
23.
24. • The attached end is called the minus end; the other
end is the plus end
• grow at the plus end by the polymerization of tubulin
dimers (powered by the hydrolysis of GTP)
Microtubules participate in a wide variety of cell
activities
• Most involve motion. The motion is provided by
protein "motors" that use the energy of ATP to move
along micotubule
25.
26. Microtubule motors
• There are two major groups of microtubule motors:
• kinesins most of these move toward the plus end of the
microtubules
• dyneins which move toward the minus end
27.
28. Some examples:
• The migration of chromosomes in mitosis and
meiosis takes place on microtubules that make up the
spindle fibres
• Both Kinesins and Dyneins are used as motors.
• The rapid transport of organelles, like vesicles and
mitochondria, along the axons of neurons takes
place along microtubules
• The motors used are kinesins
29. Vincristine, a drug found in the Madagascar
periwinkle (a wildflower), binds to tubulin dimers
preventing the assembly of microtubules. This halts
cells in metaphase of mitosis.
Taxol, a drug found in the bark of the Pacific yew,
prevents depolymerization of the microtubules of the
spindle fiber. This, in turn, stops chromosome
movement, and thus prevents the completion of
mitosis.
• Because the hallmark of cancer cells is uncontrolled
mitosis,both vincristine and Taxol are used as
anticancer drugs .
32. STRUCTURE OF MICROTUBULE
• The building blocks of microtubules are tubulin dimers
consisting of two closely related 55 kd polypeptides: α-
tubulin and β-tubulin
• Like actin, both α and β tubulin are encoded by small
families of related genes
• In addition, a third type of tubulin (y-tubulin) is concentrated
in the centrosome where it plays a critical role in initiating
microtubule assembly
• Tubulin dimers can depolymerize as well as polymerize, and
micro-tubules can undergo rapid cycles of assembly and
disassembly.
33.
34. ASSEMBLY OF MICROTUBULES
• In animal cells, most microtubules extend outward from the
centrosome
• It was first discovered by Theoder Boveri in 1888
• During mitosis Microtubules similarly extend outward from
duplicated centrosomes to form mititic spindle
• which is responsible for the separation and distribution of
chromosomes to daughter cells
35. • Microtubules grow by the addition of tubulin to
their plus ends
• which extend outward from the centrosome
toward the cell periphery, so the role of the
centrosome is to initiate microtubule growth.
• The centrosomes of most animal cells contain a
pair of centrioles, oriented perpendicular to each
other
• The centrioles are cylindrical structures based on
nine triplets of microtubules, similar to the basal
bodies of cilia and flagella.
37. • Cilia and flagella are microtubule-based projections
of the plasma membrane that are responsible for
movement of a variety of eukaryotic cells
• Many bacteria also have flagella, but these
prokaryotic flagella are quite different from those of
eukaryotes
• Bacterial flagella are protein filaments projecting
from the cell surface
38.
39. • Eukaryotic cilia and flagella are very similar
structures, each with a diameter of approximately
0.25 pm
• The fundamental structure of both cilia and
flagella is the Axoneme,which is composed of
microtubules and their associated proteins
• The microtubules are arranged in a characteristic
"9 + 2" pattern in which a central pair of
microtubules is surrounded by nine outer micro-
tubule doublets