Gene cloning involves making exact copies of a particular gene or DNA sequence using genetic engineering techniques. The key steps are: 1. Isolating the gene of interest from an organism's DNA. 2. Inserting the isolated gene fragment into a vector, such as a plasmid, to form recombinant DNA. 3. Introducing the recombinant DNA into a host cell, usually a bacterium, where it can replicate. As the host cells divide, numerous identical copies of the gene are produced. Gene cloning is used to isolate and amplify genes, determine nucleotide sequences, identify control sequences and mutations, and engineer organisms for applications like producing insulin or making crops insect-resistant.