3. Objectives
• The ability to engineer anatomically correct pieces of viable and
functional human bone would have tremendous potential for
bone reconstructions after congenital defects
• Design and Modifying Model to create Smart biomaterial
scaffolds that improve tissue regeneration
• Biocompatible and biodegradable
• Biomaterial scaffolds that are immunologically inert
• Stem cell can be patient specific using their own isolated
cells, reducing risk of rejection
3
6. Cleft lip and palate
Patients with clefts: (A) incomplete unilateral cleft of the lip, (B) unilateral cleft of the
lip, alveolus, and palate, (C) bilateral cleft of the lip, alveolus, and palate,
(D) isolated (median) cleft palate.
6
9. Care plan timetable
• birth to 6 weeks: counseling for parents, hearing test and
feeding assessment
• 3 months: surgery to repair a cleft lip
• 6-12 months: surgery to repair a cleft palate
• 18 months: speech assessment
• 3 years: speech assessment
• 5 years: speech assessment
• 8-11 years: bone graft to the cleft in the gum area (alveolus)
• 11-15 years: orthodontic treatment and monitoring jaw growth
• 18 years+: if needed, jaw surgery, lip and nose revision
surgery, and final replacements for any missing teeth
9
10. Problems
Be the angel for cleft lip and cleft palate
children: What you can do to help?
If orthodontic and the oral surgeon
treatment failure
Decreases the extent of surgery required
for repairing the lip and palate.
10
11. OUTCOME
• Developed a biomimetic scaffold for tissue
engineering
• That provides a cell-instructive structural framework
• For inducing differentiation of stem cells into
osteogenic cells.
• This porous and matrix have increaded stiffness
• Which can facilitate its use in load-bearing bone
tissue engineering.
11
16. Engineering bone grafts
• Change stem cells
into bone cells
– with proper growth
factors in cell culture
media
• This scaffold can’t be
too big or the cells
inside will die since
they will not get
enough oxygen
A 3D calcium phosphate scaffold
From Becton Dickinson
16
17. Biomimetic Platforms for
Human Stem Cell Research
Gordana Vunjak-Novakovic,Volume 8, Issue 3, 4 March 2011, Pages 252–261
17
29. Biomaterials & scaffolds for
tissue engineering
Fergal J. O'Brien, Volume 14, Issue 3, March
2011, Pages 88–95
29
30. Confocal micrograph
Fig. 1. Confocal micrograph showing osteoblast cells (green) attached to
a highly porous collagen-GAG scaffold (red).
30
31. composite scaffolds
Fig. 2. Comparative SEM images of (a) collagen-GAG (CG) scaffold (b)
hydroxyapatite (HA) and (c) composite collagen-HA (CHA) scaffold.
31
32. collagen scaffolds for
bone tissue engineering
Fig. 3. Effect of hydroxyapatite addition on (a) stiffness and (b) permeability
of collagen scaffolds.
32
33. cell-mediated mineralization
Fig. 4. Quantitative cell-mediated mineralization by osteoblasts on the CHA
scaffolds containing differing amounts of HA
33
34. degradation in rat calvarial defect
Fig. 5. Example of core degradation in a rat calvarial defect treated with a
tissue engineered collagen-calcium phosphate scaffold 4 weeks post
implantation.
34
37. conclusion
• ideal scaffold should have several
characteristics:
– (i) high porosity for cell/tissue growth,
nutrient diffusion, matrix production and
vascularization;
– (ii) controllable degradation to match
tissue growth once implanted in body
and
– (iii) reasonable mechanical strength to
match the tissues at the site of
implantation
37
palate, in vertebrate anatomy, the roof of the mouth, separating the oral and nasal cavities. It consists of an anterior hard palate of bone and, in mammals, a posterior soft palate that has no skeletal support and terminates in a fleshy, elongated projection called the uvula.The hard palate, which composes two-thirds of the total palate area, is a plate of bone covered by a moist, durable layer of mucous-membrane tissue, which secretes small amounts of mucus. This layer forms several ridges that help grip food while the tongue agitates it during chewing
glue earis fluid replace air flow in middle ear can reduce hearing,
การแก้ปัญหาเกิดเป็นที่มาของงานวิจัย if orthodontic and the oral surgeon failure
