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Hydrogel Drug Delivery System
1. P R E PA R AT I O N A N D C H A R A C T E R I S AT I O N O F
H Y D R O G E L B A S E D D RU G D E L I V E RY S Y S T E M F O R
R E L E A S I N G F LU VA S TAT I N D RU G
Under the guidance of
Dr. Smita Mohanty
Mohamed Adam.K
(Reg.No.1007108017)
3. Fluvastatin : Works against Hypercholesterolemia
Fluvastatin is a competitive inhibitor of
HMG-CoA reductase, whichmechanism is afor the conversion
The ultimate result of this is responsible reduction of the
of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) to
plasma total cholesterol which is responsible for coronary
Shorter biological half life (2.5 hrs ) and Poor bioavailability
mevalonate, a precursordisease (CAD)
artery of sterol, including cholesterol.
(20%) has necessitated the administration of
repeated (20 to o u n twice a dayh and W o rdoses
B a c k g r 40 mg d o f t ) e higher k
to obtain the desired therapeutic efficacy which often leads
Administration of statins by oral rout is associated with
to risk of toxicity.
several problems including diarrhea, constipation, indigestion
and does not usually provide rate-controlled
release or target specificity.
Structural formula for fluvastatin
4. Hydrogel : Swellable Polymeric Materials
Hydrogels are three dimensional networks of
hydrophilic polymers.
Hydrogels are water swollen polymer matrices, with a huge
tendency to absorb water. rTheir abilitya v i n g h i g h
A c r y l i c b a s e d h y d o g e l s h to swell, under
c h aphysiological conditions, o t h athemn y ideal e r
r g e t o m a s s r a t i makes n a an o t h
material forp o l y m e r s
biomedical applications .
Hydrogels can protect drugs from hostile environments, e.g.
the presence of enzymes and low pH in the stomach.
Their porosity permits loading of drugs into the gel matrix
and subsequent drug release at a pre-designed rate.
6. Materials
Acrylic acid
N,N-methylenebisacrylamide (MBA) - Cross linker
Ammoniumpersulfate (APS) - An Initiator
N,N,N,N’-tetramethylethylenediamine
(TEMED) - An Accelerator
Sodium hydroxide (NaOH) - Neutralizer
Preparation of PSA Hydrogel
Preparation
40mg of MBA were dissolved in 10ml of stock
solution (Neutralized Acrylic acid). About 4mg of APS
and 0.5 ml of TEMED were added to prepare PSA
Hydrogel.
7. Spectral Characterization of PSA Hydrogel
110
110 0 .4 % g e l
Stoc k s oln
100
100
90
90
80
80
70
1355. 6
962. 1
1060. 5
1657. 8
60
1454. 0
1313. 5
70
992. 6
1646. 1
50
1276. 0
60
%T
1402. 5
40
835. 7
50
30
Results and Discussion
1636. 7
3243. 5
1540. 7
40
20
1350. 9
1425. 9
30
10
3316. 1
0
1536. 0
20
-10
10
-20
4000 3500
3500 3000
3000 2500
2500 2000
2000 1500
1500 1000
1000 500
500
Wavenum bers (c m-1)
Wavenum bers (c m-1)
FTIR Spectra of of stock solutiongel
FTIR Spectra crosslinked PSA
When sodium acrylate is polymerized the C=C bond is
1640 cm-1, bond. This the C=C captured in the FTIR spectra
converted to C–C related towas clearlyacrylate stretch mode ; as
the size of the1420 cm-1, peak at 1645C–C. reduced with
absorption related to cm-1
progress of polymerization.
8. Effect of Crosslinker Concentration
2500
2000
0.2% CL
Swelling ratio (%)
1500
0.4% CL
1000 0.8% CL
1% CL
500
0
0 100 200 300 400
Time (min)
Swelling behavior of Poly(sodium acrylate) hydrogels
with different crosslinker concentration in distilled water at
room temperature.
9. Effect of pH on Swelling Ratio
1800.00
1600.00
pH=2
1400.00 pH=3
pH=4
1200.00 pH=5
pH=6
Swelling Ratio %
1000.00 pH=7
800.00
600.00
400.00
200.00
0.00
0.00 500.00 1000.00 1500.00 2000.00
Time (min)
Swelling behavior of Poly(sodium acrylate) hydrogels at different pH
11. Effect of Incubation Time on Drug Loading
The dependency of the drug loading amount on the
incubation time
12. Effect of Amount of Crosslinker on Drug Loading
The dependency of the drug loading amount to the
crosslinker concentration
13. Drug Release as a Function of Time
Release of fluvastatin from hydrogel carrier
as a function of time
14. Po l y ( s o d i u m a c r y l ate ) hyd ro ge l s wa s sy nt h e s i ze d
t h ro u g h c ro s s l i n k i n g o f a c r y l i c a c i d / a c r y l a m i d e .
Swe l l i n g ca p a c i t y o f t h e hyd ro ge l s i s a ffe c te d b y
t h e c ro s s l i n ke r ( M BA ) co n c e nt rat i o n , s o t h at t h e
s we l l i n g i s d e c re a s e d b y i n c re a s i n g t h e M BA
Conclusion
co n c e nt rat i o n . T h e s u p e ra b s o r b e nt hyd ro ge l s
ex h i b i te d h i g h s e n s i t i v i t y to p H , s o t h at , s e ve ra l
s we l l i n g c h a n ge s o f t h e hyd ro ge l we re o b s e r ve d
i n p H va r i at i o n s o f a w i d e ra n ge ( 2 - 7 ) a n d
effe c t i ve e n o u g h fo r d r u g d e l i ve r y.
