BITTERGOURD CULTIVATION , PRODUCTION TECHNOLOGY OF BITTER GOURDArvind Yadav
BITTER GOURD
Scientific name : Momordica charantia L.
Family : Cucurbitaceae
Chromosome number :2n=22
Origin : Tropical Asia (Eastern India and
Southern China)
Common names : Balsam pear, Bitter cucumber
Varieties:-
Pusa Do Mausmi
Pusa Vishesh
CO 1
MDU 1
COBgoH-1
VK 1 Priya
Priyanka(Sel.1010)
Arka Harit
Harkani
Phule Green
This presentation is done by 2010/2011 batch of Export Agriculture students of Uva Wellassa University of Sri Lanka as a requirement for the subject which is “Rice & Field Crop Production”. Note that the information included here is relevant to Sri Lankan condition.
BITTERGOURD CULTIVATION , PRODUCTION TECHNOLOGY OF BITTER GOURDArvind Yadav
BITTER GOURD
Scientific name : Momordica charantia L.
Family : Cucurbitaceae
Chromosome number :2n=22
Origin : Tropical Asia (Eastern India and
Southern China)
Common names : Balsam pear, Bitter cucumber
Varieties:-
Pusa Do Mausmi
Pusa Vishesh
CO 1
MDU 1
COBgoH-1
VK 1 Priya
Priyanka(Sel.1010)
Arka Harit
Harkani
Phule Green
This presentation is done by 2010/2011 batch of Export Agriculture students of Uva Wellassa University of Sri Lanka as a requirement for the subject which is “Rice & Field Crop Production”. Note that the information included here is relevant to Sri Lankan condition.
This presentation is done by 2010/2011 batch of Export Agriculture students of Uva Wellassa University of Sri Lanka as a requirement for the subject which is “Fruit & Vegetable Cultivation”. Note that the information included here is relevant to Sri Lankan condition.
“Seed priming is a controlled hydration technique in which seeds are soaked in water or low osmotic potential solution to a point where germination related metabolic activities begin in the seeds but radical emergence does not occur.”
This presentation is done by 2010/2011 batch of Export Agriculture students of Uva Wellassa University of Sri Lanka as a requirement for the subject which is “Fruit & Vegetable Cultivation”. Note that the information included here is relevant to Sri Lankan condition.
Seed dormancy is fully explained in this ppt. it includes causes ( dormancy due to hard seed coat, dormancy due to condition of embryo, dormancy due to absence of light, dormancy due to low temperature etc. ) of seed dormancy, types of seed dormancy, various methods to remove seed dormancy like impaction, stratification, scarification, exposure of seed to light
This presentation is done by Export Agriculture students of Uva Wellassa University of Sri Lanka as a requirement for the subject which is “Fruit & Vegetable production”. Note that the information included here is relevant to Sri Lankan condition.
Here i would like to inform you about different bio control agents and their mode of action, it may help you in further understanding...........................................
Foliar feeding is a technique of feeding plants by applying liquid fertilizer directly to their leaves. Plants are able to absorb essential elements through their leaves. The absorption takes place through their stomata and also through their epidermis.
This presentation is done by 2010/2011 batch of Export Agriculture students of Uva Wellassa University of Sri Lanka as a requirement for the subject which is “Fruit & Vegetable Cultivation”. Note that the information included here is relevant to Sri Lankan condition.
“Seed priming is a controlled hydration technique in which seeds are soaked in water or low osmotic potential solution to a point where germination related metabolic activities begin in the seeds but radical emergence does not occur.”
This presentation is done by 2010/2011 batch of Export Agriculture students of Uva Wellassa University of Sri Lanka as a requirement for the subject which is “Fruit & Vegetable Cultivation”. Note that the information included here is relevant to Sri Lankan condition.
Seed dormancy is fully explained in this ppt. it includes causes ( dormancy due to hard seed coat, dormancy due to condition of embryo, dormancy due to absence of light, dormancy due to low temperature etc. ) of seed dormancy, types of seed dormancy, various methods to remove seed dormancy like impaction, stratification, scarification, exposure of seed to light
This presentation is done by Export Agriculture students of Uva Wellassa University of Sri Lanka as a requirement for the subject which is “Fruit & Vegetable production”. Note that the information included here is relevant to Sri Lankan condition.
Here i would like to inform you about different bio control agents and their mode of action, it may help you in further understanding...........................................
Foliar feeding is a technique of feeding plants by applying liquid fertilizer directly to their leaves. Plants are able to absorb essential elements through their leaves. The absorption takes place through their stomata and also through their epidermis.
Seed dormancy is defined as a state in which seeds are prevented from germinating even under environmental conditions normally favorable for germination.
These conditions are a complex combination of water, light, temperature, gasses, mechanical restrictions, seed coats, and hormone structures.Seed is a ripe and fertilized ovule that encloses an embryo
It is the connecting link between parents and progeny.
Structurally seeds have four major components,
Seed coat or a protective envelope
Embryo
Reserve food and minerals which provide nourishment
Enzymes and hormones for the digestion of food and formation of new tissues during germination
Principles of seed production 1
Seed Science & Technology
K Vanangamudi
ICAR AIEEA JRF & SRF for PG admissions exams
ICAR NET, ARS & STO (T-6) exams
IBPS – AFO exams
Agronomic principles of seed production
Isolation distance in seed production
Integrated nutrient management (INM) in seed production
Roguing in seed production
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
4.
Germination
Germination in plants is the process by which a
dormant seed begins to sprout and grow into a
seedling under the right growing conditions.
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5.
external factors
Also known as environmental factors
Abiotic factors
Water
Air
Temperature
Light
Soil condition
Biotic factors
Pathogenic organisms
Internal factors
Biotic factors
Seed reserves
Dormancy
Impermeable seed coat
Other factors
Factors Responsible For
Seed Germination
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6.
Deterioration of seed
Kind/variety of the seed
Genotypic factors
Initial seed quality
Effect of organisms associated with seeds
Bacteria and fungi
Insects and mites
Fluctuating environmental conditions
Oxygen pressure
Storage life of seed
Direct sunlight exposure on the seed
Other factors
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7.
Number of times and kind of fumigation
Effect of seed treatment
Damping off of stand seedlings
Infestation of weeds
Morphological changes
Cell membrane changes
Loss of enzyme activity
Reduced respiration
Increase in seed leachates
Increase in free fatty acid content
Other factors
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8.
External factors
Abiotic factors
Water
Water is required for germination. The uptake of water
by seeds is called imbibition, which leads to the swelling
and the breaking of the seed coat.
After the seedling emerges from the seed coat and starts
growing roots and leaves, the seedlings food reserves are
typically exhausted; at this point photosynthesis
provides the energy needed for continued growth and
the seedling now requires a continuous supply of water,
nutrients and light.
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9.
Abiotic factors
Air
Germinating seeds respire at a very high rate.
The adequate supply of oxygen is necessary for
germination to take place and the seedlings to grow.
Germination rate and germination percentage of most
kinds of seeds are adversely affected if the germinating
medium is poorly aerated.
In seed-beds that are over-watered or poorly drained
with clayey soil, the soil pore spaces may be so filled
with water that oxygen supply to the seeds is retarted or
prevented.
External factors
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11.
Abiotic factors
Soil conditions
Soil salinity and poor quality water used to irrigate the
seed beds often retard or prevent germination.
Salinity problems become severe when the germinating
medium is lightly and frequently watered; evaporation
of water leaves salt deposits on the soil surface. Small
shallowly sown seeds are likely to be damaged by salts.
This problem can be solved by using soils with adequate
organic matter and irrigation water low in slats, with
holding fertilizers and by irrigating more copiously but
less frequently so that excess salts are leached out.
External factors
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12.
Abiotic factors
Light or darkness
Light or darkness can be an environmental trigger for
germination and is a type of physiological dormancy.
Most seeds are not affected by light or darkness, but
many seeds, including species found in forest settings,
will not germinate until an opening in the canopy allows
sufficient light for growth of the seedling.
External factors
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13.
Biotic factors
Pathogenic organisms
Pathogenic organisms on the seed surface, in the
germinating medium, in the irrigation water or on the
tools may cause low germination and rotting of
seedlings.
The situation is known as damping-off and is aggravated
by high moisture and high temperature condition.
External factors
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14.
Biotic factor
Seed dormancy
In some plants the embryo is not fully mature at the time of
seed shedding.
Such seeds do not germinate till the embryo attains maturity
The freshly shed seed in certain plants may not have sufficient
amounts of growth hormones required for the growth of
embryo.
These seeds require some interval of time during which the
hormones get synthesized.
The viability period ranges from a few weeks to many years.
Seeds of Lotus have the maximum viability period of 1000
years. Seeds germinate before the ending of their viability
periods.
Internal factors
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17.
Factors affecting seed viability Causes of less viability
Harvesting of seed Degeneration of enzyme
Duration between harvesting and
threshing
Disappearance of stored food
Methods of threshing Abnormal seed coat
Moisture content and relative
humidity of the stored seed
Loss of power to protect the
protoplasmic molecule from the inert
molecule
Age of seed Gradual coagulation of protein of
embryo
Temperature of the stored seed
Aeration in stored seed
Contaminate of the seed
Seed viability
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18.
Seed factor for germination Field factor for germination
viability Good tilth
vigor Optimum soil moisture
Seed age Optimum soil
temperature
Free from dormancy Aeration
Free from injury Free from obstacle
Factors for Germination
Factors for germination
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19.
Some types of seeds are inherently long lived; others
are short lived, while others have an intermediate
life span owing to their differences on genetic
makeup.
During seed production strict attention must be
given to the maintenance of genetic purity and other
qualities of seeds by introduction of new superior
crop plant varieties.
In other words, seed production must be carried out
under standardized and well-organized condition.
Genotypic factors
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20.
Deterioration of varieties
Developmental variation
Mechanical mixtures
Mutations
Natural crossing
Minor genetic variations
Selective influence of diseases
Techniques of plant breeders
Genetic principle
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21.
When the seed crops are grown
In difficult environment
Under different soil and fertility conditions
Or different climatic conditions
Or under different photoperiods
Or at different elevation for several consecutive generations
-----the developmental variation may arise some times as
different growth response
To minimize the opportunity for such shifts to occur in
varieties it is advisable to grow them in their areas of
adaptation and growing season
1-Developmental
variation
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22.
To avoid this sort of mechanical contamination it
would be necessary
To rogue the seed fields at least at the three stages
And practice the utmost care during the seed
production, harvesting, threshing and further
handling.
2-Mechanical mixtures
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23.
This is not a serious factor of varietal deterioration.
In the majority of the cases it is difficult to identify or
detect minor mutation
4-Natural crossing
In sexually propagated crops, natural crossing is
another most important source of varietal
deterioration due to introgression to genes from
unrelated stocks which can only be solved by
prevention.
3-Mutations
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24.
Natural crossing with undesirable
types
Natural crossing with diseased plants
Natural crossing with off-type plants
Natural crossing occurs
due to following three
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25.
The breeding system of species
Isolation systems
Varietal mass
Polling agent
Size of the pollen grain
Duration of pollen viability
Natural crossing occurs
due to following factors
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26.
Minor genetic variations may exist even in the
varieties appearing phenotypically uniform and
homogenous at the time of their release.
During later production cycle some of this variation
may be lost because of selective elimination by the
environment
To overcome these yields trials are suggested.
5-minor genetic
variations
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27.
The selective influence of disease in varietal
deterioration is also of considerable importance
New crop varieties often become susceptible to new
races of diseases often caused by obligate parasites
and are out of seed programs
Similarly the vegetatively propagated stocks
deteriorate fast if infected by viral, fungal and
bacterial diseases.
During seed production it is, therefore, very
important to produce disease free seeds/stocks.
6-selective influence of
diseases
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28.
In certain instances, serious instabilities may occur in
varieties due to cytogenetically irregularities not
properly assessed in the new varieties prior to their
release.
Other factors, such as
Breakdown in male sterility
Certain environmental conditions
And other heritable variations
-------May considerably lower the genetic purity.
7-Techniques of plant
breeders
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29.
Most important source of variety deterioration take place
At sowing time if more than 1 variety is sown with same
drill
During harvesting & threshing operations
Gunny bags, seed bins, elevators, etc. are connected with
seeds of other varieties
Solution
rogue the seed fields
Care during seed production, harvesting, threshing &
further handling.
Mechanical mixtures
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30.
The presence of weed seeds at time of crop harvest
leads to mixing of weed seeds with crop seeds. In
many instances it is difficult to remove them during
the processing of seeds.
The presence of weeds in the seed field or nearby
areas may serve as host to no. of diseases. Good &
effective weed control therefore would be necessary
to obtain good seed yields & to avoid contamination.
Weed control
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31.
Faulty germination and wide gap cause weed crop
competition intense
Weed seeds germinate readily while crop emergence
at longer intervals, which leads to severe weed crop
competition, hence adequate seed rate, use of good
quality seed and growing quickly germinating crop
in weed susceptible crop in weed susceptible area,
have to be practiced.
Weed crop interference
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32.
It has been recognized as a definite disease
responsible for poor germination and stand of
seedling in nursery bed.
The disease may be physiological(caused by
physiological disorder) or Pathogenic(caused by
microorganisms mostly fungi).
The seedlings of ornamental and vegetable plants,
field crops, and forest trees in nurseries all are liable
to be affected.
Failure of stand seedling
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