This document discusses instrumental methods of analysis used for qualitative and quantitative analysis of drugs. It describes the scope, objectives, types of analysis, and applications of instrumental analysis. The objectives are to understand interactions of matter with electromagnetic radiation and its applications in drug analysis, understand chromatographic separation and analysis of drugs, and perform quantitative and qualitative drug analysis using analytical instruments. Types of analysis include classical wet chemical methods and modern instrumental methods using techniques like chromatography, spectroscopy, and electrophoresis. Applications include biomedical analysis, pharmaceutical quality control, environmental monitoring, and forensic science.

1. Instrumental Methods of Analysis

2. CONTENTS
 Scope
 Objectives
 Types of analysis
 Applications

3. Scope
 This subject deals with the application of
instrumental methods in qualitative and
quantitative analysis of drugs.
 This subject is designed to impart a
fundamental knowledge on the principles
and instrumentation of spectroscopic and
chromatographic technique.
 This also emphasizes on theoretical and
practical knowledge on modern analytical
instruments that are used for drug testing.

4. Objectives
To understand the interaction of
matter with electromagnetic radiations
and its applications in drug analysis.
To understand the chromatographic
separation and analysis of drugs
To perform quantitative & qualitative
analysis of drugs using various
analytical instruments.

5. Instrumental methods of Analysis
 Instrumental methods of Analysis studies and uses
instruments used to separate, identify,
and quantify matter.
 In practice, separation, identification or
quantification may constitute the entire analysis or
be combined with another method.
 Separation isolates analytes

6. Qualitative & quantitative analysis
Qualitative analysis identifies
analytes
quantitative analysis determines
the numerical amount or
concentration.

7. TYPES OF ANALYSIS

8.  Analysis consists of classical, wet chemical
methods and modern, instrumental
methods.
 Classical qualitative methods use
separations such as precipitation, extraction
and distillation.
 Identification may be based on differences
in color, odor, melting point, boiling point,
solubility, radioactivity or reactivity.
 Classical quantitative analysis uses mass or
volume changes to quantify amount.

9.  Instrumental methods may be used to
separate samples
using chromatography, electrophoresis or fie
ld flow fractionation.
 Then qualitative and quantitative analysis
can be performed, often with the same
instrument and may use light
interaction, heat interaction, electric fields
or magnetic fields.
 Often the same instrument can separate,
identify and quantify an analyte.

10. APPLICATIONS
 Analytical chemistry has played critical roles in the
understanding of basic science to a variety of practical
applications, such as
 biomedical applications
 Pharmaceutical products
 environmental monitoring
 quality control of industrial manufacturing
 forensic science

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