Stem cells in regenerative biology and medicinePasteur_Tunis
Présentation réalisée par Shahragim Tajbakhsh durant le cours du réseau international des instituts Pasteur de "Médecine Génomique: du diagnostic à la thérapie " (17-21 octobre 2016)
Stem cells in regenerative biology and medicinePasteur_Tunis
Présentation réalisée par Shahragim Tajbakhsh durant le cours du réseau international des instituts Pasteur de "Médecine Génomique: du diagnostic à la thérapie " (17-21 octobre 2016)
Introduction to Cancer
Stem cells and cancer cells
major pathways that lead to formation of tumors.
Tumor Supressors
Colon cancer to prove Knudson hypothesis.
The modern treatments available to treat cancer.
Genome: The entire chromosomal genetic material of an organism.
Sequencing a genome: Determining the identity and order of nucleotides in the genetic material – usually DNA, sometimes RNA, of an organism.
Deciphering DNA sequences is essential for virtually all branches of biological research. With the
advent of capillary electrophoresis (CE)-based Sanger sequencing, scientists gained the ability to
elucidate genetic information from any given biological system. This technology has become widely
adopted in laboratories around the world, yet has always been hampered by inherent limitations in
throughput, scalability, speed, and resolution that often preclude scientists from obtaining the essential
information they need for their course of study. To overcome these barriers, an entirely new technology
was required—Next-Generation Sequencing (NGS), a fundamentally different approach to sequencing
that triggered numerous ground-breaking discoveries and ignited a revolution in genomic science.
A detail ppt about Genome organization with focus on all levels of organization. Most recent research and findings about CT is also added in this ppt. Detail account of 30nm fiber and its ultra structure and types is also included.
Molecular diagnosis of genetic disease ppt for studentsthirupathiSathya
DEFINITION:
Dna analysis can be used for the identification of carriers of hereditary disorders.
For prenatal diagnosis of serious genetic conditions yearly diagnosis before the onset of symptoms is done MOLECULAR DIAGNOSIS OF GENETIC DISEASE
CYSTIC FIBROSIS:
Cystic fibrosis is a genetic disease that affect mostly lungs and also the pancreas.
Screening test:
It is a complex process
Large number of genetic alterations have to be done.
For eg : It is the one of the most common lethal autosomal recessive disorder in Europe.
It is caused by mutations to cystic fibrosis transmembrane conductance regulator(CFTR) gene .
Screening individuals who may be at risk for cystic fibrosis for 500 different mutations is a daunting task.
Diagnosis test that screen for a large number of mutations of a single gene in one assay being developed.
SICKLE CELL ANEMIA:
It is a disorder where red blood cells become rigid and sticky and are shaped like “sickle”.
This irregularly shaped cells stucks in small blood vessels which can slow and block the blood flow and oxygen to all the parts of the body.
There’s no cure for sickle cell anemia.
Screening for sickle cell anemia:
SCA is a genetic disease that is the result of a single nucleotide change in the codon for the sixth aminoacid of the β- chain of the hemoglobin molecule.
The anemia is caused by the inability of the mutated hemoglobin to carry sufficient oxygen.
Target – probe hybridasation is done.
In shotgun sequencing the genome is broken randomly into short fragments (1 to 2 kbp long) suitable for sequencing. The fragments are ligated into a suitable vector and then partially sequenced. Around 400–500 bp of sequence can be generated from each fragment in a single sequencing run. In some cases, both ends of a fragment are sequenced. Computerized searching for overlaps between individual sequences then assembles the complete sequence.
Introduction to Cancer
Stem cells and cancer cells
major pathways that lead to formation of tumors.
Tumor Supressors
Colon cancer to prove Knudson hypothesis.
The modern treatments available to treat cancer.
Genome: The entire chromosomal genetic material of an organism.
Sequencing a genome: Determining the identity and order of nucleotides in the genetic material – usually DNA, sometimes RNA, of an organism.
Deciphering DNA sequences is essential for virtually all branches of biological research. With the
advent of capillary electrophoresis (CE)-based Sanger sequencing, scientists gained the ability to
elucidate genetic information from any given biological system. This technology has become widely
adopted in laboratories around the world, yet has always been hampered by inherent limitations in
throughput, scalability, speed, and resolution that often preclude scientists from obtaining the essential
information they need for their course of study. To overcome these barriers, an entirely new technology
was required—Next-Generation Sequencing (NGS), a fundamentally different approach to sequencing
that triggered numerous ground-breaking discoveries and ignited a revolution in genomic science.
A detail ppt about Genome organization with focus on all levels of organization. Most recent research and findings about CT is also added in this ppt. Detail account of 30nm fiber and its ultra structure and types is also included.
Molecular diagnosis of genetic disease ppt for studentsthirupathiSathya
DEFINITION:
Dna analysis can be used for the identification of carriers of hereditary disorders.
For prenatal diagnosis of serious genetic conditions yearly diagnosis before the onset of symptoms is done MOLECULAR DIAGNOSIS OF GENETIC DISEASE
CYSTIC FIBROSIS:
Cystic fibrosis is a genetic disease that affect mostly lungs and also the pancreas.
Screening test:
It is a complex process
Large number of genetic alterations have to be done.
For eg : It is the one of the most common lethal autosomal recessive disorder in Europe.
It is caused by mutations to cystic fibrosis transmembrane conductance regulator(CFTR) gene .
Screening individuals who may be at risk for cystic fibrosis for 500 different mutations is a daunting task.
Diagnosis test that screen for a large number of mutations of a single gene in one assay being developed.
SICKLE CELL ANEMIA:
It is a disorder where red blood cells become rigid and sticky and are shaped like “sickle”.
This irregularly shaped cells stucks in small blood vessels which can slow and block the blood flow and oxygen to all the parts of the body.
There’s no cure for sickle cell anemia.
Screening for sickle cell anemia:
SCA is a genetic disease that is the result of a single nucleotide change in the codon for the sixth aminoacid of the β- chain of the hemoglobin molecule.
The anemia is caused by the inability of the mutated hemoglobin to carry sufficient oxygen.
Target – probe hybridasation is done.
In shotgun sequencing the genome is broken randomly into short fragments (1 to 2 kbp long) suitable for sequencing. The fragments are ligated into a suitable vector and then partially sequenced. Around 400–500 bp of sequence can be generated from each fragment in a single sequencing run. In some cases, both ends of a fragment are sequenced. Computerized searching for overlaps between individual sequences then assembles the complete sequence.
Presentation examining the track and field events from a strength coach's perspective. Part of a presentation I did at the 2013 Australian Track and Field Coach's Association's Coaching Congress.
This PPT describes about the Metabolic response to injury as given in Bailey & Love - 26th edition. It will be very useful for Final year MBBS students.
Cellular Response to cell Injury and Cellular Adaptations .pptxRukhshanda Ramzaan
Cellular Adaptations
ability of cells to respond to various types of stimuli and adverse environmental changes
Atrophy(decrease in cell size)
Hypertrophy(increase in cell size)
Hyperplasia(increase in cell number)
Metaplasia(change in cell type): Replacement of one differentiated cell type with another mature differentiated cell type that is not normally present in that tissue.
Dysplasia (increase in abnormal cell): Dysplasia is not cancer, but it may sometimes become cancer.
Terminologies used in Disturbances of Cell Growth which are not adaptations
Agenesis refers to the failure of an organ to develop during embryonic growth and development
Hypoplasia is the underdevelopment or incomplete development of a tissue or organ. It is technically not the opposite of hyperplasia (too many cells). Hypoplasia is a congenital condition, while hyperplasia generally refers to excessive cell growth later in life.
Aplasia is a birth defect where an organ or tissue is wholly or largely absent. It is caused by a defect in a developmental process.
A good review of common GYN/ONC chemotherapy agents, especially for residents. Given at Wake Forest. Thanks to Dr. Michael Kelly for contributing and reviewing.
18. Wong & Booth, 1990 Sedentary Control No Reps Mod Freq/Mod Resistance 24 Reps, 500 gms High Freq/ Low Resistance 192 Reps, 0 gms High Freq/ High Resistance 192 Reps, 800-1100 gms
19.
20. Results: Exercise Protein Synthesis DNA Content Muscle Hypertrophy Concentric Increase Increase None Eccentric Increase No Increase Increase