2. Sampling Technique and Sampling
Form
Purpose of Proper Sampling Technique –
• To determine the Chemical, Physical and
Biological characteristic of water in respect to its
suitability for a particular purpose such as
cooking, drinking for the protection of human
health or suitable for aquatic plant & animal
trough analysis.
• Timely detection and/or prediction of drinking
water contamination are critically important in
protecting the environment and the economy.
3. Purpose of Proper Sampling Technique
• Result of water quality analysis will be valueless if
the samples are not properly collected and stored. It
has important consequences for sampling rules,
sampling procedures and method of sample
preservation and storage. To use of recommended
sample bottle with proper clean / sterile, use of
proper preservatives during sampling, minimize
sample transportation time etc are the factor to
produce authentic water quality test results.
4. Type of container for Sample
Collection
• Sample should be collected by :
- Borosilicate Glass ware
- High density Polyethylene bottle
- Other Specified Plastic Container
-Sample bottle must be Cleaned and sterile.
5. Labeling of Sample Container
• Sample should be labeled properly and it should
be water proof
Label should have the following information:
• Tube well ID
• Sample number
• Collection date
• Collection time
• Collection place (GPS Reading)
• Preservative if any.
• Well depth.
6. Contents of Sampling Form
• Sampling form should have the following information:
• Tube well ID
• Caretakers name
• Address
• Age of the well
• GPS Reading
• Parameter to be analyzed
• Well depth
• Name and signature of the sample collector
• Send the sampling form together with the sample to the
respective laboratory
7. Activities During Sample Collection
• Wash your hand with the well water. Uncap the
sample bottle. Do not touch inside of the bottle
or cap and do not put down either the cap or the
bottle until the sampling is completed.
• Rinse the bottle and cap three times with the
well water (Before sample collection) coming
from the spout by filling the bottle half full, cap it
and shake it. Do not rinse the bottle in the
bucket water.
8. • At first, fill up relevant information in the
sample collection form
• Take GPS Reading and record it.
• Pump the well minimum for five minutes.
• Now measure Temperature, pH, color,
Taste, Odor, turbidity, EC, TDS, Salinity
with the help of portable meter and record
it in the "Sample Collection Form".
9. Sample Storage & Transportation
• The time between Sampling and analysis
should be kept to a minimum. However, if
the sample is required to store it should
be:
• At low temperature (4˚ to 10˚ C) in the dark
place.
• Time of transport should be less than 4 hours for
Microbiological test.
10. Process of Preservative Use
• Fill the container to within 2-3 cm of the
top and add 6-8 ml Conc. HCL or HNO3/L
to the Sampling bottle for pH <2. Add
additional water until the bottle is full to the
brim and cap tightly.
12. Sample Collection for
Bacteriological Analysis
• Sterilize the pump outlet for a minute with the flame by a
gas burner, lighter or an ignited alcohol-soaked cotton-
wool swab.
• Again Pump the tube well and allow the water to flow for
one-two minutes at a medium flow rate. Unscrew the cap
of the sterilized sampling bottle. Immediately hold the
bottle under tube well outlet (spout) and fill with water. A
small air space should be left to make shaking before
analysis. Screw the cap and keep in the sample box with
ice pack).
• Time of transport should be less than 4 hours or Cool
box with ice pack or portable Microbiological Testing
instrument should be carry in the field to conduct the
Bacteriological test.
13. Sampling Form
Name of the project:
Organization:
Sample No.: Date: Time:
Mouza: Union: Upazila: Dist:
Latitude: N: Longitude: E:
Sample Type: GW SW
Source: DW/HTW/STW/DTW/MW/PW River/Pond/Lake
Depth: Model of collection: Pumped/Sampler
Onsite Measurements:
Temperature: 0
C EC: µS/cm
pH:
DO mg/L%
Samples: F/A F/UA UF/UA
Onsite observations:
Sample collected by: Supervised by:
