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Dechlorination of Polychlorinated Biphenyl (PCB) Congeners by  Dehalococcoides By: David VanHoute First Year Graduate Student Rensselaer Polytechnic Institute 11/12/2010
PCBs ,[object Object],[object Object],[object Object],[object Object],[object Object]
Genus Dehalococcoides ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Reductive Dehalogenating Enzymes (RDases) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Rotation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
PCB Calibration Standards ,[object Object],[object Object],[object Object]
Methyl Viologen Assays (MV) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Collecting Data ,[object Object],[object Object]
Assessing Dechlorination   ,[object Object],[object Object],[object Object]
Significant Dechlorination of Congener 234 CB ,[object Object],[object Object],[object Object],[object Object],[object Object]
Significant Dechlorination of Congener 2345 CB ,[object Object],[object Object],[object Object]
Positive Control of 236 CB What Happened? ,[object Object],[object Object],[object Object]
Conclusions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Future? ,[object Object],[object Object],[object Object],[object Object]
Acknowledgements ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
 

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Rotation I Presentation

  • 1. Dechlorination of Polychlorinated Biphenyl (PCB) Congeners by Dehalococcoides By: David VanHoute First Year Graduate Student Rensselaer Polytechnic Institute 11/12/2010
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.  
  • 17.  

Editor's Notes

  1. Stress need for terminal electron acceptor; specific Rdase enzymes, N and P process generally preferred.
  2. 36 in others
  3. Anoxic reagents, anaerobic chamber, crimp and purge vials, limit oxygen We know that Dehalococcoides dechlorinates mixtures of PCBs, but don’t know which congeners.
  4. Retention times show how long specific congeners remain on GC column. Shows where product is seen and substrate
  5. MV provides ample supply of H, no need for hydrogenase activity by microbes.
  6. Both 234 and 2345 are doubly flanked congeners, significant product with removal of meta chlorine.
  7. 234 and 2345 are doubly flanked chlorines that get removed, don’t really know why.