Protozoa are single-celled eukaryotic organisms that were first discovered by Anton van Leeuwenhoek in the 17th century. They exhibit a variety of life forms including free-living, symbiotic, commensal, and parasitic. Protozoa are microscopic in size, ranging from 0.002 mm to 16 mm. They have primitive cellular organization and perform all life activities within a single cell. Reproduction can occur asexually through binary fission or multiple fission, or sexually through conjugation of gametes.
This is for FYBSc students of University of Mumbai, Maharashtra, India, studying in course one semester I.
For further query you may email at sudesh_rathod@yahoo.co.in
This is for FYBSc students of University of Mumbai, Maharashtra, India, studying in course one semester I.
For further query you may email at sudesh_rathod@yahoo.co.in
This PPT is for F.Y.B.Sc students of course I Semester I, belonging to Mumbai University of Maharashtra India.
You can email at sudesh_rathod@yahoo.co.in for further query.
This PPT is for F.Y.B.Sc students of course I Semester I, belonging to Mumbai University of Maharashtra India.
You can email at sudesh_rathod@yahoo.co.in for further query.
Harsh billore (cell the functional unit of life & cellular organisation)Harsh Billore
cell definition, its theory,size and shape , different types of cell, basic difference between prokaryotic and eukaryotic cells; basic difference between unicellular and multicellular organism ; difference between animal and plant cells.
This pdf is about the Schizophrenia.
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THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
2. INTRODUCTION
• These organisms were first discovered by Anton
Van Leuven Hoek(1632-1723)
• It includes free and parasitic forms
• Some live in soil and most in fresh water
• Goldfus in 1820 named them as PROTOZOA
• Protos= First; Zoan= Animal
• The morphological structure was defined by Von
Sie Bold in 1845. He identified them as single
celled organisms
• Dobell called them as acellular
3. SPECIAL CHARACTERS
1. Division of labour is found among organelles
which are intracellular
2. Cell division (Mitosis) of metazoans is exhibited
in asexual reproduction
3. All the life activities are carried out by single cell
4. Response to stimulii can be correlated to the
biginning of nervous system
5. Conjugants in Paramecium indicates the
beginning of sexual dimorphism
4. General Characters
Microscopic, primitive animalcules
Acelluar or single celled animals with protoplasmic
grade of organisation
Solitary or colonial forms, free living symbionts,
commensals or parasites
Distributed all over the world in water, soil, air, on
animals, and inside the animals
Round, spherical, flat in shape
Symmetry is either bilateral or radial or spherical or
asymmetric
5. General Characters contd..
Size varies from 0.002 mm to 16mm, a fossil Nummulite
is large and measured about 19 cm
Body- Naked/pellicle/shells- Shells are made up of
mucus/ cellulose/ organic, inorganic material and sand
particles
All life activities are performed by single cell. Various cell
organelles are responsible for different activities
Cell membrane may be thin or thick, may have lines,
fibres and myonemes
Myonemes are useful for contraction and expansion and
also for supporting . Microvilli are useful for transport of
food material.
6. General Characters contd..
Locomotion is by pseudopodia, flagella, cilia. These
help in capturing food material
Cell contains one or two or more nucleii. Macro
nucleus is for metabolic activities and micronucleus is
for reproduction
These are phytotrophic, holozoic, holophytic,
saprophytic, saprozoic or parasitic.
Respiration is by simple diffusion. Parasites are
anerobes
Excretion is by general body surface/contractile
vacuoles-osmoregulation too. Marine and parasitic
organisms lack contractile vacuoles.
7. General Characters contd..
Reproduction is by asexual or sexual
Asexual reproduction is by binary fission/
multiple fission/plasmotomy- cytoplasmic
division to form two or more multicellular
daughter cells
Sexual reproduction is by conjugation or fusion of
gametes
These originated in precambrian of paleozoic era.
Nearly 30,000 protozoans are recognised so far.