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Xinxiang Medical University | 12 April 2024
Harold James Doroteo, RN, MPH, FRSPH
Introduction
• Proteomics is the study of all proteins
expressed by a cell, tissue, or organism,
including their structures, functions,
interactions, and modifications. It involves
the comprehensive analysis of the proteome,
which refers to the entire set of proteins
present in a biological sample at a specific
time under defined conditions.
Introduction
• Proteomics aims to understand the complex
networks and dynamics of proteins within
biological systems and their roles in various
cellular processes, such as signaling pathways,
metabolic pathways, and gene regulation.
THE DIFFERENCE BETWEEN GENOMICS AND
PROTEOMICS
THE DIFFERENCE BETWEEN GENOMICS AND
PROTEOMICS
THE DIFFERENCE BETWEEN GENOMICS AND
PROTEOMICS
THE DIFFERENCE BETWEEN GENOMICS AND
PROTEOMICS
Importance of Protein
Detection
• Discussion on the significance of protein
detection in understanding cellular processes,
disease mechanisms, and drug discovery.
• Examples of applications of protein detection
technology in various fields, including
biomedical research, clinical diagnostics, and
pharmaceutical development.
Activity Sheet # 1:
Traditional Protein Detection
Methods
• Provide examples of traditional protein
detection methods
Activity Sheet # 2:
Mass Spectrometry (MS)
• Mass spectrometry is a powerful tool for
protein identification and quantification.
Illustrate the workflow:
Activity Sheet # 3:
Gel-Based Proteomics
• Provide examples of gel-based proteomics
techniques
Activity Sheet # 4:
Emerging Protein Detection
Technologies
• Provide examples of emerging protein detection
technologies
Proteomics: Protein Detection Technology
Proteomics: Protein Detection Technology
Proteomics: Protein Detection Technology
Proteomics: Protein Detection Technology

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Proteomics: Protein Detection Technology

  • 1. Xinxiang Medical University | 12 April 2024 Harold James Doroteo, RN, MPH, FRSPH
  • 2. Introduction • Proteomics is the study of all proteins expressed by a cell, tissue, or organism, including their structures, functions, interactions, and modifications. It involves the comprehensive analysis of the proteome, which refers to the entire set of proteins present in a biological sample at a specific time under defined conditions.
  • 3. Introduction • Proteomics aims to understand the complex networks and dynamics of proteins within biological systems and their roles in various cellular processes, such as signaling pathways, metabolic pathways, and gene regulation.
  • 4.
  • 5. THE DIFFERENCE BETWEEN GENOMICS AND PROTEOMICS
  • 6. THE DIFFERENCE BETWEEN GENOMICS AND PROTEOMICS
  • 7. THE DIFFERENCE BETWEEN GENOMICS AND PROTEOMICS
  • 8. THE DIFFERENCE BETWEEN GENOMICS AND PROTEOMICS
  • 9. Importance of Protein Detection • Discussion on the significance of protein detection in understanding cellular processes, disease mechanisms, and drug discovery. • Examples of applications of protein detection technology in various fields, including biomedical research, clinical diagnostics, and pharmaceutical development.
  • 10.
  • 11.
  • 12. Activity Sheet # 1: Traditional Protein Detection Methods • Provide examples of traditional protein detection methods
  • 13. Activity Sheet # 2: Mass Spectrometry (MS) • Mass spectrometry is a powerful tool for protein identification and quantification. Illustrate the workflow:
  • 14. Activity Sheet # 3: Gel-Based Proteomics • Provide examples of gel-based proteomics techniques
  • 15. Activity Sheet # 4: Emerging Protein Detection Technologies • Provide examples of emerging protein detection technologies

Editor's Notes

  1. Western blotting, ELISA (Enzyme-Linked Immunosorbent Assay), and immunohistochemistry.
  2. Sample preparation, ionization, mass analysis, and data interpretation.
  3. SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) and 2D-PAGE (Two-Dimensional Polyacrylamide Gel Electrophoresis)
  4. proximity-based assays, aptamer-based assays, and nanopore sensing.