2. Introduction
• Proteomics is the study of all proteins
expressed by a cell, tissue, or organism,
including their structures, functions,
interactions, and modifications. It involves
the comprehensive analysis of the proteome,
which refers to the entire set of proteins
present in a biological sample at a specific
time under defined conditions.
3. Introduction
• Proteomics aims to understand the complex
networks and dynamics of proteins within
biological systems and their roles in various
cellular processes, such as signaling pathways,
metabolic pathways, and gene regulation.
9. Importance of Protein
Detection
• Discussion on the significance of protein
detection in understanding cellular processes,
disease mechanisms, and drug discovery.
• Examples of applications of protein detection
technology in various fields, including
biomedical research, clinical diagnostics, and
pharmaceutical development.
10.
11.
12. Activity Sheet # 1:
Traditional Protein Detection
Methods
• Provide examples of traditional protein
detection methods
13. Activity Sheet # 2:
Mass Spectrometry (MS)
• Mass spectrometry is a powerful tool for
protein identification and quantification.
Illustrate the workflow:
14. Activity Sheet # 3:
Gel-Based Proteomics
• Provide examples of gel-based proteomics
techniques
15. Activity Sheet # 4:
Emerging Protein Detection
Technologies
• Provide examples of emerging protein detection
technologies
Editor's Notes
Western blotting, ELISA (Enzyme-Linked Immunosorbent Assay), and immunohistochemistry.
Sample preparation, ionization, mass analysis, and data interpretation.
SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) and 2D-PAGE (Two-Dimensional Polyacrylamide Gel Electrophoresis)
proximity-based assays, aptamer-based assays, and nanopore sensing.