This document summarizes how to perform a protein-DNA interaction practical using online tools DNABindR and DP-Bind. It outlines submitting a query to DNABindR, viewing the results which identify potential DNA binding sites on a protein, and submitting the job to DP-Bind to get consensus predictions from multiple models on whether the protein is DNA binding or not. The document concludes by thanking the audience for their attention.

1. S.Prasanth Kumar, Bioinformatician Molecular Interaction Protein-DNA Interaction : Practical S.Prasanth Kumar Dept. of Bioinformatics Applied Botany Centre (ABC) Gujarat University, Ahmedabad, INDIA www.facebook.com/Prasanth Sivakumar FOLLOW ME ON ACCESS MY RESOURCES IN SLIDESHARE prasanthperceptron CONTACT ME [email_address]

2. Protein-DNA Interaction Practical Query Used

3. DNABindR Homepage

4. DNABindR Submission

5. DNABindR Results

6. DNABindR Results Potential DNA binding site on protein

7. DNABindR Results (truncated) (truncated) Potential DNA binding site on protein

8. DP-Bind Homepage & Submission Form

9. DP-Bind Results

10. DP-Bind Results Continued (truncated) Rule: If (SVM prediction && KLR prediction && PLR prediction == 1) Strict Consensus =1

11. Thank You For Your Attention !!!