The document describes the Biacore Molecular Interactions Shared Resource (BMISR) and its capabilities. The BMISR uses a Biacore T-100 system to detect and characterize molecular interactions in real-time without labelling. It can be used to determine if and how strongly two molecules interact, identify binding partners, and characterize affinity and kinetics. Examples of applications mentioned include studying protein-DNA, small molecule-protein, protein-peptide, and protein-RNA interactions.

1. Biacore Molecular Interactions Shared Resource (BMISR) Aykut Uren, MD, Director [email_address]

2. BMISR Have you ever wondered if two molecules directly interact with each other ? If they do, how strong is that interaction? Were you frustrated because you didn’t have the necessary technology or expertise to make it happen?

3. What can you use? Proteins DNA RNA Small molecules

4. What can you do? Detect Binding Yes/No Identify Specificity Binding Partners Characterize Affinity Kinetics Concentration Thermodynamics

5. Biacore T-100 Integrated Microfluidics Gold Sensor Chip SPR Detection System

6. Biacore T-100 Real Time See binding as it happens Unique binding information (on- and off-rates) for any size of biomol ecule Study weak or fast interactions Label Free No labels required Measure binding properties of unmodified substances Reduce time and workload Contract Free Non-invasive Measure opaque or colored samples

7. Sample Applications (Chang) Protein DNA interactions : DNA repair enzymes binding to DNA Transcription factors binding to DNA Small Molecule Protein Interactions: Small molecule inhibitors of enzymatic activities Small molecule inhibitors of protein- protein interactions Protein peptide interactions Protein RNA interactions

8. Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01 In your publications, remember to mention support provided from the GHUCCTS.