This document describes SOILEX® biotechnology for decontaminating soil, groundwater, and water surfaces. It was developed in 1996 by JSC POLYINFORM and uses specific microbe associations to break down a wide variety of pollutants like oil, heavy metals, and organic contaminants. The biotechnology involves mechanical, agrochemical, sorption, and biological processes. SOILEX® biopreparations are produced from POLYINFORM's collection of over 100 environmental microorganisms and can degrade over 95% of contaminants like black oil within a few months of use.

1. Decontamination of soil,
ground water and water surfaces
according biotechnology SOILEX®
Address: Ligovsky pr., 254, St. Petersburg, Russia, 191119 Tel.: (+7-812) 458-85-73
E-mail: info@polyinform.com Tel.: (+7-812) 458-85-74
URL: http://www.polyinform.ru Fax: (+7-812) 458-85-76

2. HISTORY
1989 JSC POLYINFORM was established. The main activities were Investigation of open pits of building materials,
carrying out engineering-geological and hydro-geological researches for a substantiation of ecologically pure
construction of industrial and civil objects.
1992 Establishing of the R&D Department and microbiological lab. Research in the field of biological treatment and
remediation of soil and water.
1996 The complex biotechnology SOILEX® was developed for cleaning of oil contaminated soil, water and water surface.
The technical solution which were the essence of the technology (the method of working, biopreparation producing,
the bio-products themselves) were protected by 16 patents of Russia.
1998 Entering the foreign market. The works on recultivation oil polluted soil at Klaipeda petroleum terminal, Lithuania
were carried out as well as clearing of soil, polluted by mineral oil, polyaromatic connections and heavy metals on
objects for NKK CORPORATION, Japan.
2003 We received USAID (USA) grant as the winner of competition "Researches and development in priority directions of
development of a science and techniques". We won the competition "Research and organizational support of large
venture projects" in the frames of Federal Target Scientific and Technical Program "R&D in priority areas of science
and technology in 2002-2006”.
2008 Designing and construction of the polygons and special yards for processing of oil sludge at the objects of fuel-gas
complex.
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3. SCIENTIFIC AND TECHNICAL BASE
Highly qualified staff
7 doctors of sciences
The team of more than 40 professionals the high education level of whom
allows solving any problem
Technical base
Chemical and microbiological labs
The production line for producing a biological product
Equipment including pumps for oil and oil products gathering (skimmers),
hydrodynamic techniques for the tanks cleaning, cleaning the outer water
pipes and mini technique
Special site for oil waste processing
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4. SOILEX® BIOTECHNOLOGY
In the basis of the technology are:
SPECIFIC ASSOCIATION MICROBES-DESTRUCTORS,
EFFECTIVE FOR A WIDE SPECTRUM OF POLLUTANTS
The technology includes the following activities:
mechanical, agro-chemical, sorption, biological.
The technical solutions which are the
POLYINFORM owns one of the largest private collection of the essence of the technology are protected by
microorganisms of the environmental safety target, including more than 100 16 patents of Russia
cultures, selected from the contaminated places located in different climatic
zones.
The collection is registered in World Data Centre for Microorganisms WFCC
WDCM 772 Japan, 28.01.98
The applied technology received the positive conclusion of the Natural
Recourses Ministry of Russia.
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5. PRINCIPLE OF BIOPREPARATION ACTIVITY
H H
CO2
H C C H
H H
C C C
O2 + BIO-PREPARATION
C C
H H H
C H
H H H2
O
Bacterial strains of the biological product used hydrocarbons and heavy fractions of petroleum as an energy source for their
livelihood.
During the first day after introduction of a biological product microorganisms are activated, there is an increase of their amount
and an irreversible process of decomposition of petroleum products to environmentally safe non-toxic products of bacterial
metabolism begins.
After the decomposition of mineral oil the mass of micro-organisms not provided with nutrient die off, being a food for
intensifying indigenous microflora.
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6. GENERAL CHARACTERISTIC OF SOILEX® BIOPREPARATION
The basis of SOILEX® biopreparation are aerobian hydrocarbons oxidizing non-pathogenic
bacteria sorbed on the ecologically clean inert support.
Constituent microbial association possesses high activity relative to some hydrocar-bons
(crude oil, diesel fuel, technical oils, heavy oil and mazut fractions) in the wide pH (4,5-8,2)
range.
SOILEX® biopreparations includes vegetative bacteria’ cells related to mesophilic bacterium
(maximum activity under high positive temperature 10-40С) and psychro-philic organisms
(maximum activity under low positive temperature 3-10С). This provides wide temperature
range of biopreparation activity. Also biopreparation in-cludes nutrient medium debris,
biogenic salts and inert support.
The titer of viable microorganisms cells not less then 1 x 1010 cells in 1 g of biopre-
parations.
Visually the preparation is friable powder of dark-brown color without characteristic odor.
The preparation is non-toxic, has no carcinogenic and cumulative effect, fire and ex-plosive
safe, and belongs to IV hazard class.
The preparation must be stored in hermetic plastic sachet under the temperature from +1 to
+20C. That provides saving of the titer of viable cells.
The warranty working life of the dry form of SOILEX® biopreparation is one year.
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7. PRODUCING OF SOILEX® BIOPREPARATION
The technological scheme
Producing of a biological product takes place
on site from seed: specially selected active
The inoculum I receiving Microorganisms -
microbial associations from our own collection nutrient medium in 10 tubes destructors of
and isolated from the occurrence of pollution 19/6 hydrocarbons
that are optimal for this type of pollution and
natural-climatic zones.
The inoculum II receiving
As a result: nutrient medium by mixing
№7 V ≤ 5 l.
- Repeatedly increases the bio-purification
efficiency,
- Independence of the biological products
suppliers as well as of the quality of supplied The inoculum III
nutrient medium receiving in the apparates V = 180 l
materials
nutrient medium Fermentation V = 4,5 m³.
SOILEX® BIOPREPARATION
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8. PURIFICATION EFFICIENCY OF SOILEX® BIOTECHNOLOGY
SOILEX® biotechnology is efficient for wide spectrum of contaminates
Salts of heavy metals:
Cooper - 60 %
Zinc - 60 %
Lead - 50 %
Arsenic - 50 %
Cadmium - 30 %
Contamination with black oil - control
Organic contamination: sample, 2 months after the contamination
Phenol – 90 %
Creosote – 80 %
Chlorbenzene - 80%
Oil contamination:
Black oil – 95 %
Technical oil – 97 %
Benzine – 98 %
Contamination with black oil - test sample,
2 months after the beginning of the
treatment
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9. EFFICIENCY OF SOILEX® BIOTECHNOLOGY
16,0
oil products concentration,
g/kg After 3 months the
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treatment efficiency
14 12,0 for oil-contaminated
soil and
12
groundwater is 80-
8,0
10 8,0 90%.
6,0
8
6 4,0 3,0
5,0 2,0
4
2,5
3,0 1,8 1,0
3,0 1,0 0,5
2
1,5 0,5
Black oil
Р4
1,5 0,7
0 0,5 0,18 Р3
technical oil
0,2 0,15 0
1
Initial 2 0,15 0
Р2
Diesel fuel
30 days 3
concentration 60 days
90 days
4 0 Р1
5 Benzine
120 days 6
180 days 7 days
240 days
The dynamics of reduction of various types of petroleum products in soil and ground water
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10. ADVANTAGES OF SOILEX® BIOTECHNOLOGY:
Short time necessary for decontamination (3-8 months);
Absolutely environmental friendly technology;
High destructive activity in the wide pH (4,5-8,5) and
temperature range (3 – 40 С);
Ability to remove heavy metals including mercury as well as
nitrates;
Adaptability to specific needs;
Full rehabilitation of the environment at the contaminated
territory
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11. SOILEX® BIOTECHNOLOGY
SCHEME OF WORKS
I. OBJECTS INVESTIGATION
The treated object Qualitative and quantitative Microbiological and Choosing the technological
characteristics indicators of oil pollution agrochemical parameters of scheme
treated soil and water
II. ISOLATION OF THE CONTAMINATION
By booms, oil catchers and filters at the water surfaces By sorbents and borders at the soil
III. MECHANICAL TREATMENT METHODS
Excavation of oil-contaminated soil, pumping of free oil, the removal of Construction of the polygons for oil waste processing
vegetation, placement it at special polygons
IV. SORPTION METHODS
Gathering oil from the surface of water, snow and soil
V. BIOLOGICAL METHODS
Treatment of the contaminated ecological systems with SOILEX® accomplished with the agrotechnical activities
VI. CHEMICAL AND MICROBIOLOGICAL CONSTANT CONTROL
Handing the object to the customer (independent expertise)
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12. SOILEX® BIOTECHNOLOGY
STAGES OF WORKS
I. DETAILED ENGINEERING INVESTIGATION
1. The site inspection
2. Engineering and land inspection
3. Geological engineering inspection
Engineering investigation and
4. Engineering and environmental inspection (sampling of contaminated sampling
soil and water)
II. ANALYSIS
Laboratory microbiological and chemical investigations
III. DESIGN WORK
1. Drawing 3D map of the contamination
Strains selection
2. Selection of the optimal treatment scheme
3. Breeding and selection of useful microorganisms strains-destructors,
effective for this type of pollution
4. Choosing remediation strategy
5. Development and coordination of the decontamination project
6. Producing of a biological product
Producing of the bio-
preparation 12

13. SOILEX® BIOTECHNOLOGY
DRAWING 3D MAP OF THE CONTAMINATION
General scheme of the contamination Stratified 3D map of the pollution
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14. SOILEX® BIOTECHNOLOGY
STAGES OF WORKS
IV. FIELD WORKS
Aeration
Applying of the texture agent (if necessary)
plowing (aeration)
Bio-preparation applying
Watering and fertilizers applying
monitoring
V. HANDING THE OBJECT TO THE CUSTOMER
SOILEX® and fertilizers (N - nitrogen; P
- phosphorus; K - potassium) applying
Control sampling
Independent expertise
Watering 14

15. TECHNOLOGICAL SCHEME OF SOIL AND GROUND WATER
TREATMENT (depth up to 5 m)
Contamination concentration
Low Medium High
Less than 50 g/kg from 50 to 150 g/kg More than 150 (up to 500) g/kg
SOILEX® and fertilizer (NPK) Applying of the texture agent for Pumping out the free oil
applying, aeration and watering concentration decreasing
Oil recovery
Oil sludge to the
Clean environment special recultivation
site
Stock-tank
oil
The residual contamination with
the low concentration
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16. TECHNOLOGICAL SCHEME OF WATER SURFACES TREATMENT
Contamination concentration
Low Medium High
Less than 30 mg/l From 30 up to 80 mg/l More than 80 (up to 500) mg/l
SOILEX® AQUA at the special Sorbent applying for Pumping out the free oil (booms,
carrier applying concentration decreasing skimmers)
Oil recovery
Medium
Clean environment concentration
Stock-tank
oil
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17. EXPERIENCE
More than 22 years at the environmental safety market
More than 500 ha of contaminated soil were cleaned
More than 220 000 m3 of oil sludge were processed
Object: "Diamonds of Russia-Sakha"России-Саха
More than 15 000 m3 of oil contaminated soil were treated
Before the treatment The treatment process After the treatment
June July August
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18. OIL POLLUTED SOIL REMEDIATION
Liquidation of the accidental black oil and diesel fuel spill at the rail way station Bologoe (the customer
- OAO "Russian Railways")
• The oil film at the water surface was from 1 to 5 mm
• The concentration of oil products in water reached 300 mg/l.
• The length of the trench was 2000 m.
Before the treatment Sorbents applying After the treatmnet
the cleaning was executed by biological method combined with the mechanical one and the sorption
technologies
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19. ACCIDENTAL OIL SPILLS RESPONSE
The train crash at the rail way station Ujta (the customer - - OAO
"Russian Railways")
• The spill of 80 t of black oil
• About 6 he contaminated
Before the treatment
As a result of the executed works the vegetation was almost completely
restored, the oil film from water surface was removed.
After the tratment
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20. LIST OF MAJOR EXECUTED PROJECTS OF POLYINFORM IN THE
SPHERE OF REMEDIATION PERFORMED DURING 1994 - 2010
total contract Duration
# Contractor Contract Object/Location Scope of work
amount, USD started completed
JSC "Russian Сleaning and recultivation of oil-polluted soil on
1 № Д94/05-01 Ujta railway station $1 378 375 15.05.1994 30.10.1994
railways" the site of 8 hectares
Д№03/05-01/
JSC "Russian
493/ТЧ-18; Locomotive depot at Dno Utilization of oil-contaminated soil (4800 m3) on
2 railways", Vitebsk $3 388 934 15.10.1998 30.12.2006
Д№05/03-07/ railway station the recultivation site (3 hectares)
branch
522/ТЧ-18
JSC "Russian
№Д№1/12-98, № Koptevskij open pit near Сleaning and recultivation of soil oil-polluted by
3 railways", Kujbyshev $6 839 673 30.12.1998 30.11.2005
488 Ul’janovsk the accidental split of 80000 tons of oil
branch
№Д03/01-05;
JSC "Russian №Д04/02-03;
Locomotive depot at Velikie Utilization of oil-contaminated soil (5000m3) on
4 railways", Vitebsk №Д04/02-02; $3 082 393 01.03.1999 30.12.2005
Luki railway station the recultivation site (3200 m2)
branch Д№05/03-05/
524/ТЧ-18
JSC "Russian № Д04/03-08, №
Locomotive depot of Suojarvi Utilization of oil-contaminated soil (2500 m3) on
5 railways", Д05/04-06; $2 348 237 01.04.1999 31.10.2006
railway station the recultivation site (1800m2)
Petrozavodsk branch №Д06/03-14
NK «UKOS»,
petroleum storage depot near Cleaning of ground water on the territory of
6 «Samaranefteproduct Д№ 1-11-99 $6 527 500 01.11.1999 31.12.2002
Tol'jatti petroleum storage depot (11 hectares)
» Corp
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21. LIST OF MAJOR EXECUTED PROJECTS OF POLYINFORM IN THE
SPHERE OF REMEDIATION PERFORMED DURING 1994 - 2010
№03/01-01,
«Orenburgneft» Corp. Liquidation of slime barns and recultivation of
7 «TNK-BP» Corp. №Д03/02-02, $2 537 926 01.02.2003 10.11.2004
(Orenburg) contaminated soil (2600 m3)
№Д03/12-02
JSC "Russian Locomotive depot of
Utilization of oil-contaminated soil (2000m3) on the
8 railways", № Д04/03-06 Medvezhja gora railway $2 373 136 01.04.2003 31.10.2006
recultivation site (1800 m2)
Petrozavodsk branch station
"Saratovneftegaz" Cleaning and recultivation of oil-contaminated site 2,1
9 №Д03/02-03 NK "Sidanko" (Stavropol') $520 255 01.05.2003 30.09.2003
Corp. hectares)
JSC "Russian station-to-station block Cleaning and recultivation of water and soil on the site
10 railways", №Д03/04-06 Station “791 km” – oil- contaminated because of the train crush (240 tons $346 939 15.07.2003 30.10.2003
Petrozavodsk branch Shueretskaja of oil-contaminated soil)
№Д03/07-04/
"Privolzhskie trunk oil-
11 "Transneft" Corp. 200300449 Cleaning of oil-contaminated sites (3,9 hectares) $1 162 857 15.08.2003 30.10.2004
pipelines" Corp (Saratov)
14.08.03
«Heat network»,
«Heat network», Puskino, Liquidation of accidental black-oil split on the boiler
12 Puskino, Moskow №Д03/05-05 $122 449 01.09.2003 30.11.2003
Moskow oblast plant (150 m3 of liquid waste and 430 m3 of soil)
oblast
Liquidation of contamination of groundwater on the
Ministry of Defense Object “Pribylovo”
13 Д№3/003/Р site of 3,4 hectares and recultivation of 420 tons of $3 412 433 01.10.2003 30.08.2006
of Russia (Leningradskaja oblast)
polluted soil
Liquidation of contamination of groundwater on the
Ministry of Defense Object “Savvatija”
14 Д№2/003/Р site of 2,5 hectares and recultivation of polluted soil $5 489 737 01.10.2003 30.11.2007
of Russia (Archangelskaja oblast)
(accidental split of 2800 tons of oil)
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22. LIST OF MAJOR EXECUTED PROJECTS OF POLYINFORM IN THE
SPHERE OF REMEDIATION PERFORMED DURING 1994 - 2010
Elimination of contamination (3950 m3) and
Ministry of Defense of Object "Kozelsk"
15 дог. № 1/003/Р recultivation of soil on the site of 6,6 $4 353 457 01.10.2003 30.11.2007
Russia (Kaluzhskaja oblast)
hectares
Д№03/10-02/
JSC "Russian railways", 496/ТЧ-18; Locomotive depot at Pskov Utilization of oil-contaminated soil (810
16 $1 153 446 15.10.2003 30.12.2005
Vitebsk branch 1Д№05/03-04/ railway station m3) on the recultivation site (2220 m2)
525/ТЧ-18,
Konakovskaja
Utilization of oil-contaminated soil (750
17 "6 Sigma" Corp. № Д04/02-01 hydroelectric plant $465 306 01.03.2004 31.10.2004
tons) on the recultivation site
(Konakovo)
Locomotive depot of
JSC "Russian railways", Disposal of oil-contaminated soil (2500 m3)
18 № Д04/03-03 Petrozavodsk railway $1 366 437 01.04.2004 31.10.2006
Petrozavodsk branch on the recultivation site (1900m2)
station
«Saratovneftegaz» Corp. Recultivation of the oil-contaminated soil on
19 «Saratovneftegaz» Corp. № 974-ПО $816 494 25.08.2004 30.11.2004
(Saratov) the site of 1,8 hectares
Kirovsky plant (St- Cleaning of oil-contaminated territory of the
20 «Krov-Oil» Inc. № Д04/10-02 $80 612 12.11.2004 30.11.2004
Petersburg) plant (180 m3 of soil)
JSC "Russian railways",
21 №Д04/10-01 Sortavala railway station Constructing of local treatment plant $842 850 01.03.2005 31.08.2005
Petrozavodsk branch
Disposal of black oil-contaminated soil (265
JSC "Russian railways", № Д05/04-04, Locomotive depot of
22 m3) on the recultivation site of locomotive $255 680 01.06.2005 31.10.2006
Petrozavodsk branch №Д06/03-15 Sortavala railway station
depot
22

23. LIST OF MAJOR EXECUTED PROJECTS OF POLYINFORM IN THE
SPHERE OF REMEDIATION PERFORMED DURING 1994 - 2010
Cleaning of 2160 tons of oil-
JSC "Russian railways", St- recultivation site of Dno contaminated soil from the area of train
23 № Д05/07-03 $4 365 913 21.07.2005 31.12.2006
Petersburg branch railway station crash on station-to-station block
Zubtsov-Aristovo
Recultivation of the territory of
24 "Aspect" Ltd Д07/01-01 "Aspect" Ltd (Kem') petroleum storage depot (1546 m3 of $1 306 486 27.02.2007 10.10.2007
contaminated soil)
“Gazpromneft-Hantos” Ltd Cleaning and recultivation of oil-
25 “Gazpromneft-Hantos” Ltd Д07/07-04 $1 835 145 10.08.2007 15.09.2008
(Hanty-Mansijsk) polluted soil (7,8 hectares)
Treatment of oil contaminated soil and
ground water at the territory of the
26 «Rosneft-Tuapse Refinery» № Д09/11-05 «Rosneft-Tuapse Refinery» Refinery with SOILEX biotechnology, 05.03.2010 In process
the depth of contamination up to 17 m,
square - 2 he
“Rosneft-Stavropolneftegaz” “Rosneft-Stavropolneftegaz” Construction of site for disposal of oil
27 Д09/01/01 $ 210 360 780 10.03.2009 15.11.2010
Ltd Ltd (Neftekumsk) slime with the square of 3.5 ha
23

24. LIST OF PATENTS, CERTIFICATES AND LICENSES OF POLYINFORM
GENERAL:
1. Certificate № 159409, «POLYINFORM» service mark, the State register of the trade marks 19.12.97
2. Certificate № 163656, «POLYINFORM» service mark, the State register of the trade marks 30.04.98
3. Federal Service for ecological, technological and nuclear control, License №ОТ-19-0000190 (78) on realization of
activities on collecting, using, neutralization, transportation, placing of hazardous waste, 10.03.2009
4. Federal Service for Supervision of the protection of cultural heritage law compliance, License № РОК 00613 on
realization of activities on the restoration of the cultural heritage objects (monuments), 17.04.2009
5. The self-regulating organization Non-Commercial Partnership "The Baltic association of designers”, Certificate №
0577-2009-78161187545-02 on admission to work on preparation of project documents, which have an impact on the
safety of capital construction, 23.12.2009
6. The self-regulating organization Non- Commercial Partnership "The Baltic Constructing Complex”, Certificate №
0577-2009-78161187545-02 on admission to the works that have an impact on the safety of capital construction,
30.12.2009
7. NP, «Regional Engineering and Surveying Association», Certificate of admission to the works that have an impact
on the safety of capital construction № 0121-FROM-2010-7816118754-01, 26.03.2010
24

25. LIST OF PATENTS, CERTIFICATES AND LICENSES OF POLYINFORM
BIOTECHNOLOGY SOILEX®:
1. Certificate № 159410, “SOILEX” trade mark, the State register of the trade marks 19.12.97
2. Certificate № 162746, “SOILEX” Trade mark, the State register of the trade marks 31.03.98
3. The patent for the invention of the Russian agency for patents and trade marks, the method of cleaning soil and water
from pollution with oil and oil minerals, the State register of the inventions
4. The patent for the invention of the Russian agency for patents and trade marks, the method of purification of the
capillary-porous environment from pollution with oil and oil minerals, the State register of the inventions
5. The patent for the invention of the Russian agency for patents and trade marks, the method of purification the capillary-
porous environment from pollution with oil and oil minerals, the State register of the inventions
6. The patent for the invention of the Russian agency for patents and trade marks, the biological product for purification of
the environment from oil and oil minerals, the State register of the inventions
7. The patent for the invention of the Russian agency for patents and trade marks, the biological product for purification of
the environment from oil and oil minerals, the State register of the inventions
8. The patent for the invention of the Russian agency for patents and trade marks, the bacteria Serratia odorifera A 3п
ГКМ ВИЗР №99 for oxidation of the Hydrocarbons of oil and oil minerals, Moscow
25

26. WE MAKE THE WORLD
ECOLOGICALLY SOUND!
Address: Ligovsky pr., 254, St. Petersburg, Russia, 196084 Tel.: +7(812) 458-85-73
E-mail: info@polyinform.com Tel.: +7(812) 458-85-74
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URL: http://www.polyinform.com Fax: +7(812) 458-85-76