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Prof S S Jahagirdar,
Associate Professor,
N K ORchid College of Engg and Tech,
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Associate Professor,
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9. Measurements of Gross Organic
Content
Dissolved Oxygen (DO)
Biochemical oxygen demand (BOD)
Chemical oxygen demand (COD)
Total organic carbon (TOC)
Theoretical oxygen demand (ThOD)
10.
11. Biological Oxygen Demand (BOD)
BOD:Oxygen is removed from water when
organic matter is consumed by bacteria
Lowoxygen conditions may kill fish and other
organisms
http://www.lcusd.net/lchs/mewoldsen/Water_Pollution_LCHS.ppt
12. Chemical Oxygen Demand
The quantity of oxygen used in biological and non-biological
oxidation of materials in wastewater
The determination of chemical oxygen demand (COD) is used
in municipal and industrial laboratories to measure the
overall level of organic contamination in wastewater. The
contamination level is determined by measuring the
equivalent amount of oxygen required to oxidize organic
matter in the sample
BOD/COD ratio – the greater the ratio, the more oxidizable
(biologically treatable) the waste. Ratios rarely exceed 0.8-
0.9.
13. Total Organic Carbon (TOC)
Measure of WW pollution characteristics
Based on the chemical formula
Test methods use heat and oxygen, UV radiation,
and/or chemical oxidants to convert organic
carbon to carbon dioxide, which can then be
measured
Can be assessed in 5 to 10 minutes
Theoretical > Measured
14. Theoretical Oxygen
Demand (ThOD)
WW generally contains a mixture of carbon,
hydrogen, oxygen, and nitrogen
Calculated using stoichiometric equations
Considers both carbonaceous and nitrogenous
oxygen demand
Main difference from COD
16. 1) Clarification, Sedimentation, Flocculation are used for
suspended and/ or colloidal pollutants
2) Evaporation, Reverse Osmosis etc, are used for
dissolved inorganic pollutants
3) Oxidation/ Synthesis by Micro-organisms is carried
out (Biological Treatment) for Dissolved Organic
Pollutant
17. Biological Processes...
cell: derives energy from oxidation of reduced
food sources (carbohydrate, protein & fats)
Requires…..
microbes with the ability to degrade the waste organics
contact time with the organics
favorable conditions for growth
18. Objective of Biological
Wastewater Treatment
To stabilize the organic matter (Soluble and
none settleble)
To reduce the amount of dissolved
phosphorus and nitrogen in the final effluent
20. Microorganisms = single-celled organism capable
of performing all life functions independently
Basic unit of life = cell
Enzymes Cell Wall
C, N, P Wastes
H2 O H2 O
O2 CO2
24. Shape of Bacteria
Cocci
– spherical cells, often in chains or tetrads
Rods
– most common shape
– vary in shape & size
Spiral Rod
– curved rods
25. Growth
Growth = cell division
one cell divides to produce two equal daughter
cells
Generation time
length of time required for bacterial population
to double
26. Bacterial Growth: cell division
single cell
cell elongates
cell produces cell wall
two daughter cells produced
The cell replicates all its components, reorganizes it into
two cells, forms a cell wall, and separates.
27. Growth curve (typical phases of
growth)
stationary phase
cell count
log phase
death phase
lag phase
time
28. Composition of bacterial cell:
Percentage by weight
Carbon 50 Potassium 1
Oxygen 20 Sodium 1
Nitrogen 14 Calcium 0.5
Hydrogen 8
Magnesium 0.5
Phosphorus 3
Iron 0.2
All other elements 0.3
Sulfur 1
Cell Formula C60H84N12O24P
29. Requirements for Bacterial
Growth
Nutritional
◦ Carbon source (waste to be degraded )
◦ N & P (100:5:1;C:N:P)
◦ Trace minerals
Environmental
◦ Oxygen (terminal electron acceptor)
◦ Temperature
◦ Water
◦ pH
◦ Non-toxic
30. Microorganisms
Classification:
Heterotrophic- obtain energy from oxidation of organic
matter (organic Carbon)
Autotrophic- obtain energy from oxidation of inorganic
matter (CO2, NH4, H+ )
Phototrophic- obtain energy from sunlight
32. Biological Treatment
In the case of domestic wastewater treatment, the objective of
biological treatment is:
To stabilize the organic content
To remove nutrients such as nitrogen and phosphorus
Types:
Attached Growth
Aerobic Processes
Suspended Growth
Anoxic Processes
Combined Systems
Anaerobic Processes
Combined Aerobic-Anoxic-
Anaerobic Processes
Aerobic
Pond Processes Maturation
Facultative
Anaerobic
33. Major Aerobic Biological Processes
Type of Common Name Use
Growth
Suspended Activated Sludge (AS) Carbonaceous BOD removal (nitrification)
Growth
Aerated Lagoons Carbonaceous BOD removal (nitrification)
Attached Trickling Filters Carbonaceous BOD removal. nitrification
Growth
Roughing Filters (trickling Carbonaceous BOD removal
filters with high hydraulic
loading rates)
Rotating Biological Carbonaceous BOD removal (nitrification)
Contactors
Packed-bed reactors Carbonaceous BOD removal (nitrification)
Combined Activated Biofilter Process Carbonaceous BOD removal (nitrification)
Suspended & Trickling filter-solids contact
Attached process
Growth Biofilter-AS process
Series trickling filter-AS process
37. What are the forms of nitrogen found in
wastewater?
Forms of nitrogen:
Organic N
TKN
Ammonia Total
Nitrite N
Nitrate
38. Why is it necessary to treat the forms
of nitrogen?
Improve receiving stream quality
Increase chlorination efficiency
Minimize pH changes in plant
Increase suitability for reuse
Prevent NH toxicity
4
Protect groundwater from nitrate contamination
Increases aquatic growth (algae)
Increases DO depletion
39. How is N removed or altered by
secondary (biological) treatment?
Biological
assimilation
BUG = C60H86O23N12P
0.13 lb N/lb of bug mass
Biological
conversion by nitrification
and denitrification
40. Nitrification
NH + Nitrosomonas NO2-
4
NO - Nitrobacter NO -
2 3
Notes:
Aerobic process
Control by SRT (4 + days)
Uses oxygen 1 mg of NH + uses 4.6 mg O
4 2
Depletes alkalinity 1
mg NH4+ consumes 7.14 mg alkalinity
Low oxygen and temperature =
difficult to operate
41. Denitrification
NO3- denitrifiers (facultative bacteria) N2
gas + CO2 gas
Notes:
Anoxic process
Control by volume and oxic MLSS recycle to anoxic
zone
N used as O2 source = 1 mg NO3- yields 2.85 mg O2
equivalent
Adds alkalinity 1 mg NO3- restores 3.57 mg
alkalinity
High BOD and NO3- load and low temperature =
difficult to operate
43. Biological Nitrogen Removal
factors affecting nitrification
temperature
substrate concentration
dissolved oxygen
pH
toxic and inhibitory substances
NH 4 − N DO 0.095(T −15)
µ = µm ⋅ ( e )[1 − 0.83(7.2 − pH )]
K N + NH 4 − N K O + DO
44. Biological Nitrogen Removal
Denitrification
Nitrate is used instead of oxygen as terminal electron acceptor
Denitrifiers require reduced carbon source for energy and
cell synthesis
Denitrifiers can use variety of organic carbon source - methanol, ethanol and
acetic acid
NO -3 → NO -2 → NO → N 2 O → N 2
NO -3 + 1.08CH 3OH + H + → 0.065C5 H 7 O 2 N + 0.47N 2 + 0.76CO 2 + 2.44H 2 O
47. Activated Sludge Process
There are two phases to biological treatment
◦ “Mineralization” of the waste organics producing
CO2 + H2O + microbes
◦ Separation of the microbes and water
48. Activated Sludge Process
Q Q+R Q-W
Aeration basin Clarifier
Clarifier (OUT)
(IN) (MLSS)
R
(RAS)
W (WAS)
49. Definitions: (measurement & control )
MLSS / MLVSS ( active microbes )
F / M ( food to mass )
RAS / WAS ( recycle & waste )
MCRT ( sludge age )
DOUR / SOUR ( how active? )
SVI / SSV30 ( settleability)
51. MLVSS
Mixed Liquor Volatile Suspended Solids
The part of MLSS which will combust.
A good approximation of the active
biological portion of the MLSS (75 - 85%)
In a well oxidised sample,
MLVSS = biomass
52. Food to Mass Ratio
F + M + O2 M + CO2 +H2O
F/M = kg /day BOD5
kg MLVSS
Concept: Microorganisms work best
with an optimum amount of food
53. Recycle (RAS)
Recycle converts once-through system into
Activated Sludge
Clarifier separates solids (biomass), thickens
and allows return of microorganisms (RAS)
Recycle or clarifier underflow influences
thickening and mass balances
Retention Time of Biomass no longer limited by
Hydraulic Retention time
54. Wasting (WAS)
Biomass is created as the microorganisms grow
= Sludge Yield (kg/kg-deltaBOD)
Sludge Yield varies by type of waste and
operating conditions (e.g. growth rate)
For Equilibrium conditions, Yield, or Excess
Sludge must be removed or Wasted
Excess sludge can involve significant influent
inert TSS
55. Sludge Age or MCRT
MCRT = Mean Cell Residence Time
System level, or Gross parameter
One definition is “average time biomass stays
in the system”
Calculated by Total Solids in System / Total
Solids being Wasted
Mathematically = 1/net growth rate
56. MCRT =
[MLVSS (ppm) * Aeration Vol. (m3)]
___________________________________
[TSS(ppm) * Eff (m3/d)]
+
[RAS MLVSS (ppm) * WAS (m3/d)]
or MCRT = Total Solids/ Wasted Solids
In practice, MLSS usually used
57. Oxygen Uptake
DOUR and SOUR or Respiration Rate (RR)
can provide useful information on health of
biomass compared to normal operation
High F/M operation = high growth rate =
High RR (e.g. 20+ mg/l/hr/g/VSS)
Extended Aeration (“old” sludge) = Low F/M
= “Low” RR (e.g. 3- 12 )
Do not confuse RR with total O2 demand
59. SOUR-- Why do it?
Indicates the health of the bugs
Can show if there is a toxin the basin
Has been shown to be correlated to the final
effluent COD so it can be used as an indication of
the effluent quality during an upset or change in
operating conditions.
60. SVI
SVI = SSV30(ml) *1000
MLSS (mg/L)
= Volume occupied by 1 gm of MLSS after 30 min of
settling (usually 1 L sample)
SSV30 = Sludge settled volume after 30
minutes in ml/L
61. SVI target 50 - 150
1000 ml
Aeration
MLSS Effluent
Sludge
Sludge Recycle
62. Information from Settling Tests
(SVI)
Graph of Rate of Settling = age of sludge
Supernatant Condition = cloudy, ash, pin floc
Production of Gas after 1- 2+ hr = denitrification
Floating material, SVI = Filamentous Bulking
Colour of Sludge e.g. brown or gray
Shape of curve and Expected RAS concentration
63. Example of Settling Data
1200
1000 Young bugs - poor settling,
potential for carryover
800
Normal Bugs, good settling CaseA
SSV (ml)
600 and good water quality CaseB
CaseC
400
200
Old Bugs- fast settling, pin floc carryover potential
0
0 5 10 15 20 25 30 40 50 60 90 120 180
Time (mins)
64. Operational Parameters in
Activated Sludge Process
Nature of substrate
F/M ratio
Dissolved Oxygen
RAS
Reactor Configuration
pH
Reaction kinetics
Reactor Hydraulics
Nutrients
65. Activated Sludge Process
Monitoring
Flows
Organic Concentrations and Loadings
Solids concentrations
Settleability data
Oxygen
Dissolved oxygen (DO) in aeration basin
DOUR (Dissolved oxygen uptake rate (mg/L/hr)
Editor's Notes
cdff
Sybron Biochemical - Wastewater Unit Processes Distributor Training Seminar - 1999
Sybron Biochemical - Wastewater Unit Processes Distributor Training Seminar - 1999 Benefits : Simpleconstruction Low capital and O&M costs Simple operation Low solids production Energy recovery possible Flow and load equalization Drawbacks : Poor process control Significant odor potential Effluent normally requires additional treatment Significant land area required Typical Applications : Pretreatment of industrial wastewater prior to downstream biological treatment or municipal discharge. Pretreatment of municipal wastewater prior to facultative or aerated lagoon treatment, or natural treatment system.
Sybron Biochemical - Wastewater Unit Processes Distributor Training Seminar - 1999 Q = influent flowrate R = return sludge flowrate Q+R = Flow to 2 0 clarifier Q-W = effluent flowrate W = waste sludge flowrate MLSS mixed liquor suspended solids RAS - return activated sludge WAS - waste activated sludge
Std. methods 2540D Filtered through a std. glass fibre filter Dry to constant weight at 103 - 105 C, at least 1 hour
MLVSS Method = method 2540E Residue from MLSS test is ignited to constant weight at 550 C (usually 15 - 20 minutes fro 200 mg) Can have negative errors from appreciable volatile organics MLVSS = 75 - 85% of MLSS, but can be 50 - 95% The active MB portion of the MLVSS can be 1 - 45%, but is still the most widely used indicator Dr. Porge said act. sludge biomass = C 5 H 7 NO 2 i.e oxygen equiv= 5x32/113 = 1.42 mg O2/mg biomass
Case A = Slow settling = YOUNG, Underoxidized, Bulky Case B = Normal Case C Rapid settling = OLD, Overoxidized (maybe some denitrification apparent- more results needed)
Sybron Biochemical - Wastewater Unit Processes Distributor Training Seminar - 1999 Flows - Influent (Q), return sludge (R), waste sludge (W), effluent (Q+W). Organics - influent , pretreatment, and final effluent BOD and COD. Solids - TSS and VSS on mixed liquor (MLSS), return sludge (RAS), waste sludge (WAS). Also on influent, pretreatment effluent, and final effluent. Settleability - 30-min settling test (SV 30), sludge volume index (SVI), sludge bed depths. Oxygen - DO should be taken at different locations and times to determine if limiting conditions exist. DOUR trends provide clues for operational strategies.