The four phases of bacterial growth are lag phase, log or exponential phase, stationary phase, and death phase. In lag phase, bacteria are adjusting to their environment and producing enzymes and ribosomes. In log phase, the population grows exponentially as bacteria actively reproduce through binary fission. In stationary phase, reproduction and death rates equalize as nutrients become limited. In death phase, death outpaces reproduction and the population declines as conditions become unsuitable.
The document summarizes an experiment that tested the hypothesis that the peripheral aster spreading during metaphase determines the pattern of the cortical F-actin ring that forms prior to polar body extrusion. The experiment used two methods - treatment with lovastatin followed by washout, and treatment with cytochalasin D followed by washout - to induce multiple cortical spreading asters. Both methods resulted in the formation of multiple, overlapping F-actin rings, supporting the hypothesis that microtubules of the peripheral aster provide a positive stimulus that directs F-actin ring formation.
Environmental sensitive genetic male sterilityGokul Dhana
This document discusses environmental sensitive genetic male sterility (EGMS) systems for hybrid seed production. It defines male sterility and describes different types of male sterility including genetic male sterility (GMS) such as thermo-sensitive GMS (TGMS) and photo-period sensitive GMS (PGMS). TGMS lines are sterile at particular high temperatures during microsporogenesis, while PGMS lines are sterile under long day conditions. The document outlines the procedure for utilizing TGMS/PGMS lines in hybrid seed production and lists advantages and disadvantages of the EGMS system.
Genetic engineering is the process of manipulating genes to introduce desirable traits. It involves combining DNA from different organisms, such as inserting a gene for insulin into bacteria. The gene is inserted into a plasmid or virus vector and introduced into a host cell. This allows the production of proteins like insulin to treat diseases. While genetic engineering holds promise to treat diseases, some argue it could disrupt nature in unintended ways. Regulatory issues also exist regarding its applications and effects.
A transgenic animal is one that has had foreign DNA inserted into its genome. The first transgenic animal was a mouse created in 1982 by inserting a human growth hormone gene. Transgenic animals are created through pronuclear microinjection or stem cell methods. They have applications in medicine, agriculture, and industry. However, some argue that transgenic technology raises ethical issues.
This document discusses animal biotechnology and methods for sex selection of livestock embryos. It begins by providing context on the history of biotechnology in animal husbandry. Key methods described include artificial insemination, progesterone assays, estrus synchronization, and embryo transfer. Details are given on techniques for sexing embryos, including probes for Y-chromosomes and differences in X-linked enzymes. Challenges to the adoption of these technologies in developing countries are also summarized.
This document summarizes research into tracing the ancestry of operons in bacteria. The researcher developed a method using maximum parsimony to reconstruct ancestral gene blocks. This approach provided good visualization of the evolutionary process. Future work will account for horizontal gene transfer and gene order changes and further develop the method. The poster discusses using this approach to study the evolution of a gibberellin biosynthesis operon.
The four phases of bacterial growth are lag phase, log or exponential phase, stationary phase, and death phase. In lag phase, bacteria are adjusting to their environment and producing enzymes and ribosomes. In log phase, the population grows exponentially as bacteria actively reproduce through binary fission. In stationary phase, reproduction and death rates equalize as nutrients become limited. In death phase, death outpaces reproduction and the population declines as conditions become unsuitable.
The document summarizes an experiment that tested the hypothesis that the peripheral aster spreading during metaphase determines the pattern of the cortical F-actin ring that forms prior to polar body extrusion. The experiment used two methods - treatment with lovastatin followed by washout, and treatment with cytochalasin D followed by washout - to induce multiple cortical spreading asters. Both methods resulted in the formation of multiple, overlapping F-actin rings, supporting the hypothesis that microtubules of the peripheral aster provide a positive stimulus that directs F-actin ring formation.
Environmental sensitive genetic male sterilityGokul Dhana
This document discusses environmental sensitive genetic male sterility (EGMS) systems for hybrid seed production. It defines male sterility and describes different types of male sterility including genetic male sterility (GMS) such as thermo-sensitive GMS (TGMS) and photo-period sensitive GMS (PGMS). TGMS lines are sterile at particular high temperatures during microsporogenesis, while PGMS lines are sterile under long day conditions. The document outlines the procedure for utilizing TGMS/PGMS lines in hybrid seed production and lists advantages and disadvantages of the EGMS system.
Genetic engineering is the process of manipulating genes to introduce desirable traits. It involves combining DNA from different organisms, such as inserting a gene for insulin into bacteria. The gene is inserted into a plasmid or virus vector and introduced into a host cell. This allows the production of proteins like insulin to treat diseases. While genetic engineering holds promise to treat diseases, some argue it could disrupt nature in unintended ways. Regulatory issues also exist regarding its applications and effects.
A transgenic animal is one that has had foreign DNA inserted into its genome. The first transgenic animal was a mouse created in 1982 by inserting a human growth hormone gene. Transgenic animals are created through pronuclear microinjection or stem cell methods. They have applications in medicine, agriculture, and industry. However, some argue that transgenic technology raises ethical issues.
This document discusses animal biotechnology and methods for sex selection of livestock embryos. It begins by providing context on the history of biotechnology in animal husbandry. Key methods described include artificial insemination, progesterone assays, estrus synchronization, and embryo transfer. Details are given on techniques for sexing embryos, including probes for Y-chromosomes and differences in X-linked enzymes. Challenges to the adoption of these technologies in developing countries are also summarized.
This document summarizes research into tracing the ancestry of operons in bacteria. The researcher developed a method using maximum parsimony to reconstruct ancestral gene blocks. This approach provided good visualization of the evolutionary process. Future work will account for horizontal gene transfer and gene order changes and further develop the method. The poster discusses using this approach to study the evolution of a gibberellin biosynthesis operon.
This document discusses methods of embryo sexing. It begins with a brief history of embryo sexing and introduces invasive and non-invasive methods. For invasive methods, it describes cytological/karyotyping methods, identification of sex chromatin, use of Y-chromosome probes, and PCR. Advantages include low cost and accuracy, while disadvantages include potential harm to embryo viability. For non-invasive methods, it outlines detection of X-linked enzymes and H-Y antigens, noting advantages of maintaining embryo integrity but challenges around accuracy and availability of reagents. The document concludes by discussing applications and constraints of embryo sexing technologies.
Day 3 embryo biopsy performed opposite to the second polar body site showed higher rates of intact inner cell mass (ICM) localization within the blastocyst (82%) compared to random biopsy sites (51%). Excising specific blastomeres at the 4-8 cell stage may interfere with embryonic development by disrupting the formation of trophectoderm and ICM precursors. Targeting the biopsy site opposite the second polar body appears to better maintain the integrity and self-organization of the ICM. Further studies are needed to evaluate the impact on clinical outcomes like implantation and delivery rates.
Animal Cloning Procedure, Problems and PerspectivesShafqat Khan
Cloning in farm animals has problems and perspectives. Key issues include developmental anomalies in cloned animals, the large offspring syndrome observed in cattle and sheep clones, and safety apprehensions regarding meat and milk from cloned animals. However, cloning also has potential applications for transgenic animal production, creating disease models, bioreactors, and research into xenotransplantation. It allows the propagation of elite livestock and conservation of endangered species. Further optimization is needed to improve cloning efficiency and resolve health issues.
Transgenic animals are created through recombinant DNA technology by inserting foreign genes into the animal's genome. This is done to improve livestock, use animals as bioreactors for pharmaceutical production, and for research purposes. The main methods of creating transgenic animals are DNA microinjection, retrovirus-mediated gene transfer, embryonic stem cell transfer, and sperm-mediated gene transfer. Examples include transgenic cows that produce more nutritious milk, pigs with genes to reduce environmental pollution, and mice used widely as model organisms. While transgenic animals have benefits, there are also ethical concerns regarding animal welfare and unintended environmental impacts.
Cloning involves producing genetically identical copies of biological entities. In the 1950s, embryologists Briggs and King developed nuclear transplantation, a technique where the nucleus is transferred from one cell to an egg cell with its original nucleus removed. This revealed that nuclei from older embryos and tadpoles, when transplanted, can affect the developmental potential of the recipient egg cell. Cloning describes processes used to copy genetic material and produce clones that are identical to the original biological entity.
Trends in evolution :- synopsis :- 1. INTRODUCTION
( DEFINITION OF EVOLUTION)
2.TRENDS IN EVOLUTION
3. DEFINITION OF MOLECULAR EVOLUTION
4.GENE EVOLUTION
*Mutation
*Horizontal gene transfer
*Sexual reproduction
5.EVOLUTION OF GENE FAMILIES
(Kind of gene families)
For more you can refer to www.faunafondness.com
This document discusses animal cloning, specifically somatic cell nuclear transfer (SCNT). It provides information on the history of cloning, animals that have been cloned, the SCNT process, challenges to successful cloning including reprogramming differentiated cells, and problems seen in cloned animals including embryonic and postnatal abnormalities. Applications of cloning such as restoring endangered species, generating transgenic animals, and gene knockout in farm animals are also covered.
This document discusses macroevolution, megaevolution, and speciation. It defines macroevolution as changes that occur at or above the species level, such as the evolution of feathers in birds. Megaevolution refers to major evolutionary transitions, such as the change from single-celled to multicellular organisms. Speciation is the formation of new species, which can occur through mechanisms of allopatric, parapatric, or sympatric speciation as populations become reproductively isolated. Intrinsic reproductive isolating mechanisms that can lead to speciation include ecological, temporal, behavioral, mechanical and gametic isolation.
Transgenic animals are animals whose genomes have been altered by the addition of foreign DNA. There are three main methods for creating transgenic animals: retroviral vector method, DNA microinjection, and using engineered embryonic stem cells. Many transgenic animals have been created successfully for various purposes, including glowing zebrafish, faster growing salmon, Alzheimer's disease mouse models, and the first transgenic monkey. Transgenic technology holds promise for applications in agriculture, medicine, and industry, but also raises ethical concerns and biosafety issues.
Transgenic animals are genetically engineered to contain genes from another species. The first transgenic animal was produced by microinjecting DNA into fertilized mouse eggs. This allows the new genes to integrate into the genome and be passed to offspring. Knockout mice have a specific endogenous gene altered so it is no longer expressed normally. They are used to study gene function and model human diseases. Dolly the sheep was the first mammal cloned from an adult cell, showing that nuclear transfer can generate a live offspring genetically identical to the donor animal.
Transgenic Animals A Better Approach towards Experimentationijtsrd
Animals play a crucial role in the development of medical products from medicines to various implants and major surgical procedures. They are not approved for human use until they qualify the safety parameters in animals. The animals play a pivotal role in drug discovery and development. One of the most challenging aspects of targeting drugs for any pathological condition is the development of animal models. Animal models are nothing but an approach to replicate the general symptoms of the diseased condition which occur in humans. The whole pathophysiology cannot be replicated but, the symptoms which are known and precipitate the most in any pathological condition are positively reproduced within the animal. With the increasing need in the medical field, the paradigm is now slowly shifting towards transgenic animals as they have given a new dimension to medical sciences to study the pathophysiology of the disease rather than focusing on the symptom based analysis of the diseased condition. These genetically modified organisms have shown groundbreaking results not only in developing models for various diseases but also in developing biopharmaceuticals, receptor selective drugs, and have a huge contribution to xenotransplantation and the agriculture sector. This review article vividly describes the newer approaches that are adopted to develop transgenic animal models and also highlights certain limitations to the older approach of developing the models. Swarnima Negi | Dr. Sachdev Yadav "Transgenic Animals: A Better Approach towards Experimentation" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-2 , February 2021, URL: https://www.ijtsrd.com/papers/ijtsrd38268.pdf Paper Url: https://www.ijtsrd.com/pharmacy/pharmacology-/38268/transgenic-animals-a-better-approach-towards-experimentation/swarnima-negi
The document discusses the generation and husbandry of transgenic mice. It describes how transgenic mice are produced by injecting DNA into fertilized mouse eggs, which can integrate randomly and potentially disrupt endogenous genes. It also describes targeted "knockout" techniques using embryonic stem cells. The document provides details on planning and maintaining transgenic mouse colonies, including breeding strategies, housing, health monitoring and common pathogens.
Transgenesis techniques have evolved from early microinjection methods to more precise engineered nuclease approaches. Initial methods like pronuclear microinjection resulted in random integration with low efficiency. The development of embryonic stem cells and nuclear transfer enabled greater control over transgenic status but required extensive cloning. Newer tools like transposons, zinc finger nucleases, and CRISPR/Cas9 allow for stable, heritable integration at targeted genomic loci with higher efficiency and less mosaicism than early random integration methods. These advances facilitate the creation of transgenic and gene-edited animal models for agricultural and biomedical applications.
Transgenic manipulation of animal embryos and its applicationDeveshMachhi
INTRODUCTION
Genetic manipulation in animal for higher productivity is also called genetic engineering, refer to the alteration of the gene of an organism.
Organisms containing integrated sequences of cloned dna (transgenes), transferred using techniques of genetic engineering (to include those of gene transfer and gene substitution) are called transgenic animals.
Transgenic technology has led to the development of fishes, live stock and other animals with altered genetic profiles which are useful to mankind.Genetically modified animals are proving ever more vital in the development of new treatments and cures for many serious diseases.
Transgenesis is a radically new technology for altering the characteristics of animals by introducing the foreign genetic material.
CONTACT: devmac1323@gmail.com
Sheep named Dolly was cloned by transfer of a nucleus from a mammary (Udder) cell of an adult sheep into an egg cell.
mammary cell
Nucleus
insert into
a egg cell
First demonstration of pluripotency (totipotency) of a nucleus of a differentiated adult cell.
Cloning of dolly somatic cell nuclei
clone cattle, sheep, goats, pigs.
nuclear transfer procedures are similar.
Adult donor cells from a variety of cell types(mammary epithelial and ovarian cells, fibroblasts, lymphocytes) are isolated
Cultured and genetically modified methods.
individual donor cells are fused to an enucleated oocyte with short-duration electric pulse.
eg: two 2.5 kilovolt /cm pulses for 10microseconds
Used to fuse adult cattle fibroblasts with enucleated oocytes.
The pulses simultaneously induce cell fusion and oocyte activation.
Blastocyst stage before transferred into the uterus of a pseudopregant female.
Confirmed transgene at the time of birth
Surviving animals produced by nuclear transfer are healthy.
There, is a substantial loss of individual before and after birth some of the cloned animals display abnormalities.
Abnormlities such as increased birth weight.
Dna methylation and histone modification of the original donor cell is inappropriate maintained in the cells of the recipient animals.
Transgenic animals are animals whose genomes have been modified by the addition or deletion of genes. Common methods to produce transgenic animals include DNA microinjection, retrovirus-mediated gene transfer, and embryonic stem cell-mediated gene transfer. Transgenic animals are produced for various economic, medical, and industrial applications such as increasing milk production in cows, developing disease models, and producing pharmaceuticals in the milk of transgenic animals. Proper housing and care is required to maintain transgenic animals.
This presentation gives a comprehensive detail of transgenic animal, processes involve in the production of transgenic animal and also highlights several benefits of transgenic animal
The document defines transgenic animals as organisms with DNA from another source inserted into their genome, making them genetically modified organisms (GMOs). It provides a brief historical background on early transgenic research and commercialization. The document then categorizes GMOs, describes common methods for producing transgenic animals like embryonic stem cell and pronucleus methods, and gives examples of transgenic mice, fish, cows, pigs, monkeys, and more. It discusses advantages like improved growth and traits, as well as disadvantages like unintended effects and breeding issues. Finally, it outlines applications like producing recombinant proteins, serving as disease models, and aiding medical research.
Developmental mechanisms of evolutionary changeMerlyn Denesia
This document summarizes key concepts in developmental biology and evolution. It discusses how changes in developmental pathways and gene regulation allow for morphological evolution while maintaining homologous structures. Modularity allows changes in certain body parts without disrupting others. Development is constrained by physical laws, developmental mechanisms, and phylogenetic history. Changes accumulate over generations through mutations affecting timing, scaling, duplication, and co-option of genetic and developmental mechanisms.
- Spirulina is a blue-green algae that is highly nutritious, containing nutrients like protein, iron, beta carotene, vitamins, and minerals in high amounts. Research shows it has benefits like reducing risks of cancer, heart disease, and viruses. It is about 60% complete protein and grows rapidly with high yields. Studies indicate spirulina may help conditions like anemia, diabetes, cardiovascular issues, neurodegenerative diseases, and more due to its antioxidants and anti-inflammatory properties. In conclusion, spirulina is a very nutritious supplement that can benefit various aspects of health.
The Chernobyl nuclear disaster of 1986 caused widespread radiation exposure. Spirulina was donated and helped improve health outcomes for those affected by increasing white blood cell count and improving immunity. Spirulina is a type of blue-green algae that is highly nutritious, containing protein, vitamins, minerals, and other nutrients. It has been used successfully to treat those exposed to radiation from Chernobyl and provides many health benefits as a dietary supplement.
This document discusses methods of embryo sexing. It begins with a brief history of embryo sexing and introduces invasive and non-invasive methods. For invasive methods, it describes cytological/karyotyping methods, identification of sex chromatin, use of Y-chromosome probes, and PCR. Advantages include low cost and accuracy, while disadvantages include potential harm to embryo viability. For non-invasive methods, it outlines detection of X-linked enzymes and H-Y antigens, noting advantages of maintaining embryo integrity but challenges around accuracy and availability of reagents. The document concludes by discussing applications and constraints of embryo sexing technologies.
Day 3 embryo biopsy performed opposite to the second polar body site showed higher rates of intact inner cell mass (ICM) localization within the blastocyst (82%) compared to random biopsy sites (51%). Excising specific blastomeres at the 4-8 cell stage may interfere with embryonic development by disrupting the formation of trophectoderm and ICM precursors. Targeting the biopsy site opposite the second polar body appears to better maintain the integrity and self-organization of the ICM. Further studies are needed to evaluate the impact on clinical outcomes like implantation and delivery rates.
Animal Cloning Procedure, Problems and PerspectivesShafqat Khan
Cloning in farm animals has problems and perspectives. Key issues include developmental anomalies in cloned animals, the large offspring syndrome observed in cattle and sheep clones, and safety apprehensions regarding meat and milk from cloned animals. However, cloning also has potential applications for transgenic animal production, creating disease models, bioreactors, and research into xenotransplantation. It allows the propagation of elite livestock and conservation of endangered species. Further optimization is needed to improve cloning efficiency and resolve health issues.
Transgenic animals are created through recombinant DNA technology by inserting foreign genes into the animal's genome. This is done to improve livestock, use animals as bioreactors for pharmaceutical production, and for research purposes. The main methods of creating transgenic animals are DNA microinjection, retrovirus-mediated gene transfer, embryonic stem cell transfer, and sperm-mediated gene transfer. Examples include transgenic cows that produce more nutritious milk, pigs with genes to reduce environmental pollution, and mice used widely as model organisms. While transgenic animals have benefits, there are also ethical concerns regarding animal welfare and unintended environmental impacts.
Cloning involves producing genetically identical copies of biological entities. In the 1950s, embryologists Briggs and King developed nuclear transplantation, a technique where the nucleus is transferred from one cell to an egg cell with its original nucleus removed. This revealed that nuclei from older embryos and tadpoles, when transplanted, can affect the developmental potential of the recipient egg cell. Cloning describes processes used to copy genetic material and produce clones that are identical to the original biological entity.
Trends in evolution :- synopsis :- 1. INTRODUCTION
( DEFINITION OF EVOLUTION)
2.TRENDS IN EVOLUTION
3. DEFINITION OF MOLECULAR EVOLUTION
4.GENE EVOLUTION
*Mutation
*Horizontal gene transfer
*Sexual reproduction
5.EVOLUTION OF GENE FAMILIES
(Kind of gene families)
For more you can refer to www.faunafondness.com
This document discusses animal cloning, specifically somatic cell nuclear transfer (SCNT). It provides information on the history of cloning, animals that have been cloned, the SCNT process, challenges to successful cloning including reprogramming differentiated cells, and problems seen in cloned animals including embryonic and postnatal abnormalities. Applications of cloning such as restoring endangered species, generating transgenic animals, and gene knockout in farm animals are also covered.
This document discusses macroevolution, megaevolution, and speciation. It defines macroevolution as changes that occur at or above the species level, such as the evolution of feathers in birds. Megaevolution refers to major evolutionary transitions, such as the change from single-celled to multicellular organisms. Speciation is the formation of new species, which can occur through mechanisms of allopatric, parapatric, or sympatric speciation as populations become reproductively isolated. Intrinsic reproductive isolating mechanisms that can lead to speciation include ecological, temporal, behavioral, mechanical and gametic isolation.
Transgenic animals are animals whose genomes have been altered by the addition of foreign DNA. There are three main methods for creating transgenic animals: retroviral vector method, DNA microinjection, and using engineered embryonic stem cells. Many transgenic animals have been created successfully for various purposes, including glowing zebrafish, faster growing salmon, Alzheimer's disease mouse models, and the first transgenic monkey. Transgenic technology holds promise for applications in agriculture, medicine, and industry, but also raises ethical concerns and biosafety issues.
Transgenic animals are genetically engineered to contain genes from another species. The first transgenic animal was produced by microinjecting DNA into fertilized mouse eggs. This allows the new genes to integrate into the genome and be passed to offspring. Knockout mice have a specific endogenous gene altered so it is no longer expressed normally. They are used to study gene function and model human diseases. Dolly the sheep was the first mammal cloned from an adult cell, showing that nuclear transfer can generate a live offspring genetically identical to the donor animal.
Transgenic Animals A Better Approach towards Experimentationijtsrd
Animals play a crucial role in the development of medical products from medicines to various implants and major surgical procedures. They are not approved for human use until they qualify the safety parameters in animals. The animals play a pivotal role in drug discovery and development. One of the most challenging aspects of targeting drugs for any pathological condition is the development of animal models. Animal models are nothing but an approach to replicate the general symptoms of the diseased condition which occur in humans. The whole pathophysiology cannot be replicated but, the symptoms which are known and precipitate the most in any pathological condition are positively reproduced within the animal. With the increasing need in the medical field, the paradigm is now slowly shifting towards transgenic animals as they have given a new dimension to medical sciences to study the pathophysiology of the disease rather than focusing on the symptom based analysis of the diseased condition. These genetically modified organisms have shown groundbreaking results not only in developing models for various diseases but also in developing biopharmaceuticals, receptor selective drugs, and have a huge contribution to xenotransplantation and the agriculture sector. This review article vividly describes the newer approaches that are adopted to develop transgenic animal models and also highlights certain limitations to the older approach of developing the models. Swarnima Negi | Dr. Sachdev Yadav "Transgenic Animals: A Better Approach towards Experimentation" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-2 , February 2021, URL: https://www.ijtsrd.com/papers/ijtsrd38268.pdf Paper Url: https://www.ijtsrd.com/pharmacy/pharmacology-/38268/transgenic-animals-a-better-approach-towards-experimentation/swarnima-negi
The document discusses the generation and husbandry of transgenic mice. It describes how transgenic mice are produced by injecting DNA into fertilized mouse eggs, which can integrate randomly and potentially disrupt endogenous genes. It also describes targeted "knockout" techniques using embryonic stem cells. The document provides details on planning and maintaining transgenic mouse colonies, including breeding strategies, housing, health monitoring and common pathogens.
Transgenesis techniques have evolved from early microinjection methods to more precise engineered nuclease approaches. Initial methods like pronuclear microinjection resulted in random integration with low efficiency. The development of embryonic stem cells and nuclear transfer enabled greater control over transgenic status but required extensive cloning. Newer tools like transposons, zinc finger nucleases, and CRISPR/Cas9 allow for stable, heritable integration at targeted genomic loci with higher efficiency and less mosaicism than early random integration methods. These advances facilitate the creation of transgenic and gene-edited animal models for agricultural and biomedical applications.
Transgenic manipulation of animal embryos and its applicationDeveshMachhi
INTRODUCTION
Genetic manipulation in animal for higher productivity is also called genetic engineering, refer to the alteration of the gene of an organism.
Organisms containing integrated sequences of cloned dna (transgenes), transferred using techniques of genetic engineering (to include those of gene transfer and gene substitution) are called transgenic animals.
Transgenic technology has led to the development of fishes, live stock and other animals with altered genetic profiles which are useful to mankind.Genetically modified animals are proving ever more vital in the development of new treatments and cures for many serious diseases.
Transgenesis is a radically new technology for altering the characteristics of animals by introducing the foreign genetic material.
CONTACT: devmac1323@gmail.com
Sheep named Dolly was cloned by transfer of a nucleus from a mammary (Udder) cell of an adult sheep into an egg cell.
mammary cell
Nucleus
insert into
a egg cell
First demonstration of pluripotency (totipotency) of a nucleus of a differentiated adult cell.
Cloning of dolly somatic cell nuclei
clone cattle, sheep, goats, pigs.
nuclear transfer procedures are similar.
Adult donor cells from a variety of cell types(mammary epithelial and ovarian cells, fibroblasts, lymphocytes) are isolated
Cultured and genetically modified methods.
individual donor cells are fused to an enucleated oocyte with short-duration electric pulse.
eg: two 2.5 kilovolt /cm pulses for 10microseconds
Used to fuse adult cattle fibroblasts with enucleated oocytes.
The pulses simultaneously induce cell fusion and oocyte activation.
Blastocyst stage before transferred into the uterus of a pseudopregant female.
Confirmed transgene at the time of birth
Surviving animals produced by nuclear transfer are healthy.
There, is a substantial loss of individual before and after birth some of the cloned animals display abnormalities.
Abnormlities such as increased birth weight.
Dna methylation and histone modification of the original donor cell is inappropriate maintained in the cells of the recipient animals.
Transgenic animals are animals whose genomes have been modified by the addition or deletion of genes. Common methods to produce transgenic animals include DNA microinjection, retrovirus-mediated gene transfer, and embryonic stem cell-mediated gene transfer. Transgenic animals are produced for various economic, medical, and industrial applications such as increasing milk production in cows, developing disease models, and producing pharmaceuticals in the milk of transgenic animals. Proper housing and care is required to maintain transgenic animals.
This presentation gives a comprehensive detail of transgenic animal, processes involve in the production of transgenic animal and also highlights several benefits of transgenic animal
The document defines transgenic animals as organisms with DNA from another source inserted into their genome, making them genetically modified organisms (GMOs). It provides a brief historical background on early transgenic research and commercialization. The document then categorizes GMOs, describes common methods for producing transgenic animals like embryonic stem cell and pronucleus methods, and gives examples of transgenic mice, fish, cows, pigs, monkeys, and more. It discusses advantages like improved growth and traits, as well as disadvantages like unintended effects and breeding issues. Finally, it outlines applications like producing recombinant proteins, serving as disease models, and aiding medical research.
Developmental mechanisms of evolutionary changeMerlyn Denesia
This document summarizes key concepts in developmental biology and evolution. It discusses how changes in developmental pathways and gene regulation allow for morphological evolution while maintaining homologous structures. Modularity allows changes in certain body parts without disrupting others. Development is constrained by physical laws, developmental mechanisms, and phylogenetic history. Changes accumulate over generations through mutations affecting timing, scaling, duplication, and co-option of genetic and developmental mechanisms.
- Spirulina is a blue-green algae that is highly nutritious, containing nutrients like protein, iron, beta carotene, vitamins, and minerals in high amounts. Research shows it has benefits like reducing risks of cancer, heart disease, and viruses. It is about 60% complete protein and grows rapidly with high yields. Studies indicate spirulina may help conditions like anemia, diabetes, cardiovascular issues, neurodegenerative diseases, and more due to its antioxidants and anti-inflammatory properties. In conclusion, spirulina is a very nutritious supplement that can benefit various aspects of health.
The Chernobyl nuclear disaster of 1986 caused widespread radiation exposure. Spirulina was donated and helped improve health outcomes for those affected by increasing white blood cell count and improving immunity. Spirulina is a type of blue-green algae that is highly nutritious, containing protein, vitamins, minerals, and other nutrients. It has been used successfully to treat those exposed to radiation from Chernobyl and provides many health benefits as a dietary supplement.
This document discusses the nutritional composition and health benefits of spirulina. Spirulina is high in protein, vitamins, minerals, and bioflavonoids. It supports cell growth, immunity, and has anti-cancer, antiviral, cholesterol-lowering and anti-inflammatory properties. DXN Spirulina in particular has the highest levels of nutrients and safety standards. The document provides details on the amino acid, fatty acid, vitamin and mineral content of spirulina and how each component benefits health.
Fungal infections have increased due to medical advances and immunosuppression. They are difficult to treat because fungi grow slowly and infect tissues poorly penetrated by drugs. The main antifungal drug classes are polyenes, azoles, and echinocandins. Polyenes like amphotericin B bind ergosterol in fungal membranes, causing leakage. Azoles like fluconazole inhibit ergosterol synthesis. Echinocandins prevent fungal cell wall synthesis. Drug choice depends on infection severity, location, and fungal species. Long-term treatment is usually needed.
Gene therapy involves modifying genes to treat or cure disease. It works by replacing mutated genes, inactivating abnormal genes, or introducing new genes. Early successes treated immune deficiencies, but challenges remain in achieving long-term effects without side effects. Promising areas are treating inherited retinal diseases and Parkinson's through localized delivery of therapeutic genes using viral or non-viral vectors. While offering potential cures, gene therapy also raises ethical issues that require ongoing discussion.
The document discusses reactive oxygen species (ROS) and their effects on boar sperm physiology. It notes that ROS can negatively impact sperm motility, viability, and fertilization potential by damaging lipids, proteins, DNA, and inducing premature capacitation. This is detrimental to sperm quality and lifespan. The document also examines the use of seminal fluid diluents for short or long-term sperm preservation. Diluents aim to maintain sperm concentration and viability during storage and transport. However, ROS levels may still increase and damage sperm over time. The inclusion of macromolecules that stabilize sperm membranes could help protect sperm from ROS effects and better maintain motility and function during preservation.
The document summarizes a study that investigated how anoxia-induced suspended animation can protect model organisms from cell cycle errors caused by cold exposure. The study found that wild-type C. elegans embryos and S. cerevisiae strains exhibited high lethality and cell cycle defects when exposed to cold temperatures. However, transitioning the organisms into a state of suspended animation through anoxia before cold exposure largely prevented cold-induced viability defects and cell cycle errors. The authors provide evidence that suspended animation imposes a reversible arrest of the cell cycle, preventing aberrant cell cycle events from occurring in cold conditions.
This document summarizes a study examining the formation and function of the contractile ring involved in polar body extrusion in the surf clam Spisula. The study found that:
1) The metaphase peripheral aster spreads along the egg cortex in an umbrella-like pattern, leaving a microtubule-poor center.
2) During anaphase, the aster disassembles as a cortical F-actin ring forms that matches the location, size, and pattern of the previous aster.
3) Inhibiting F-actin or myosin blocked polar body formation, while disrupting or stabilizing asters prevented proper ring and polar body formation, supporting the hypothesis that aster spreading
1. The document discusses the recognition and interaction between eggs and sperm in various species. It describes the key steps in fertilization, including chemoattraction of sperm to eggs, the acrosomal reaction, binding to the egg's extracellular envelope, and fusion of the plasma membranes.
2. In sea urchins, a peptide called resact attracts sperm to eggs and activates them. Bindin, a protein in the acrosomal process, mediates species-specific binding to the egg.
3. In mice, the zona pellucida protein ZP3 specifically binds sperm and initiates the acrosomal reaction, allowing enzymes to penetrate the zona for fertilization.
It was observed that swim rate of sperm changes in microgravity. Sperm swim with higher velocity in microgravity. The fertilization might be affected by this mobility changes in sperm and this led to reduction in sperm motility under microgravity. Follicle Stimulating Hormone FSH that is responsible for ovulation in women by triggering egg maturity and stimulating sperm production in men can be damaged by microgravity. Therefore, ovulations and triggering of egg maturity and production of sperm may not take place. Higher acidity of outer vagina due to fluid distribution at the upper part of the body might kill the sperm or reduce sperm counts. Microgravity environment reduced the thickening of the endometrium to the extent that eggs cannot be planted cause non implantations . Jaiyeola O. Paul | Oluwafemi A. Funmilola | Abdullahi S. Ayegba "Microgravity and Infertility" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-6 , October 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29408.pdf Paper URL: https://www.ijtsrd.com/other-scientific-research-area/other/29408/microgravity-and-infertility/jaiyeola-o-paul
This document summarizes research on sperm cell motility, viability, and calcium regulation. It discusses how sperm motility is regulated by calcium levels, which are controlled by ion channels such as CATsper. Modulating calcium channels and reducing motility may increase sperm cell viability by decreasing reactive oxygen species production. The document also reviews the roles of factors like temperature, membrane potential, and ion currents in modulating calcium concentrations and sperm cell functions like capacitation and the acrosome reaction.
The document discusses the process of fertilization in several species. It describes 5 steps for recognition and fusion of egg and sperm cells, including chemoattraction, release of acrosomal enzymes, binding to extracellular envelopes, passage through envelopes, and fusion of nuclei. It also discusses the acrosome reaction, prevention of polyspermy through changes in electric potential and cortical granule reaction, and activation of the egg through calcium signaling and increased metabolism. Finally, it summarizes the process of sperm capacitation required for fertilization competence.
The biology of Aging in Insects From Drosphila to other insects and back.pptxArchana Ramanji
This particular presentation describes aging in insects and explains the mechanisms underlying it, particularly in Drosophila including eusocial insects.
Quantum entanglement is a phenomenon in theoretical physics that happens when pairs or groups of particles are generated in such a way that the quantum state of each particle cannot be described independently of the others, even when the particles are separated by a large distance. Instead, a quantum state must be described for the system as a whole. Based on the theory of cancer as an evolutionary metabolic disease (Evolutionary Metabolic Hypothesis of Cancer or EMHC), the cancerous cells are eukaryotic cells with different metabolic rate from healthy cells due to the damaged or shut down mitochondria in them. Assuming each human eukaryotic cell as a particle and the whole body as a Quantum Entangled System (QES), is a new perspective on the description of cancer disease, and this link between theoretical physics and biological sciences in the field of cancer therapies can be a new insight into the cause, prevention and treatment of cancer. Additionally, this perspective admits the Lamarckian evolution in the understanding of the mentioned disease. We have presented each human eukaryotic cell containing mitochondria as a QES, and the whole body containing healthy and normal cells as a QES as well. The difference between the entropy of the healthy cells and cancer cells has also been mentioned in this research.
Keywords: Quantum Entanglement, Cancer, Mitochondria, Evolution, Quantum Entangled System (QES), EMHC
This document summarizes current knowledge about plant hormone cross-talk during root meristem size determination. It discusses the molecular mechanisms of auxin, cytokinin, and gibberellin biosynthesis, transport, and signaling. It explains that a complex network of hormone interactions coordinates root development processes like cell division and differentiation to determine meristem size. Recent research efforts aim to define the components and dynamics of these hormonal networks through molecular and computational approaches.
Protoplasts are plant or microbial cells that have had their cell walls removed, leaving the cytoplasmic membrane as the outermost layer. Protoplast fusion involves fusing two or more protoplasts together using mechanical, chemical, or electrical methods. This technique is useful for introducing genetic recombinations to improve strains for industrial or agricultural purposes. Some examples include producing yeast hybrids with improved ethanol production or carotenoid overproduction in algae. Protoplast fusion has applications in strain improvement, developing novel antibiotics, and basic research on organelle function.
ABSTRACT- Bacterial Spores are robust and dormant life forms. The enthralling controlling system can maintain the spore dormancy for years yet allow the reappearance into active state within minutes thus provide resistance to the bacterium to heat, freezing, chemicals, radiations and other adverse environments. In spite of being considered as a spoilage and disease cause, Bacterial spores have been emerging as a miracle package. The survivability of bacterial spores under harsh conditions provides various solutions to human needs. Thus bacterial spores are drawing increased interest of the researchers as a solution to get work done under tough conditions. Bacterial spores have been exploited successfully to develop Biological Detection Systems as they can sense environmental changes and respond rapidly. Recently several spore based biosensors have been developed for the detection of different contaminants from different sources. More valued Probiotic Products based on bacterial spores have also been developed as spores can travel through GIT safely due to their resistant to digestive enzymes.Taking advantage of spore survivability, Pest Control Products based on spores are being used for making innovations in pest control. Different strains of Bacillus thuringiensis have been used to protect crops. More recent Bt genes have been expressed in transgenic plants to provide inherent resistance. Bacillus spores also have been exploited for vaccine delivery as a non- invasive and thermostable vaccine delivery system. Bioremediation and Electricity generation is also another applied corner of bacterial spores. This reevaluation highlights the potential of this simpler microbial structure and recent growth in the applied bacterial spore biology.
Key-words- Bacterial Spore, Biological detection system, Probiotics, Vaccine delivery, Bioremediation
Explants are pieces of tissue placed in culture. Callus is an unorganized mass of cells that forms from explants. There are three stages of callus culture: induction, proliferation, and differentiation. Cell suspension cultures can be initiated from callus and involve transferring callus pieces into liquid medium with agitation to break up cell aggregates. Organogenesis is the formation of organs like leaves and roots on explants, which can occur directly or indirectly through a callus stage. Somatic embryogenesis involves the formation of embryos from somatic cells and follows a similar development process to zygotic embryogenesis.
Honors - Cells & insulin, membrane and transport 1112bMichael Edgar
1) The document discusses cells called leukocytes that move from blood vessels into tissues in response to cytokines and cellular adhesion molecules on endothelial cells.
2) It shows an image of mouse pancreas cells that express the Ptf1a gene, which is essential for forming the pancreas including insulin-secreting beta cells.
3) It provides information about proinsulin, insulin-like growth factor 1, and experiments transplanting human beta cells into mice pancreases.
This document discusses various methods for the production of secondary metabolites from plant cell cultures, including cell cloning of auxotrophic cells, immobilization of plant cells, two-phase system culture, hairy root culture, and genetic engineering of plant cells. Cell cloning involves selecting high-yielding cell lines by plating single cells and screening for altered phenotypes. Immobilization techniques entrap cells in gels, nets, foams or hollow fiber membranes. Hairy root culture uses Agrobacterium rhizogenes to generate transformed root cultures for production. Selection techniques include visual analysis, chemical analysis, and selecting for resistance to toxic analogs.
Behavioural analysis of nematode movementNageshb11
1) Nematodes move through undulatory propulsion, where waves of muscular contraction and relaxation pass along the body. This allows them to move through soil, water films, plant tissues, and other environments.
2) Nematodes use sensory inputs like chemosensilla and mechanosensilla to detect chemicals, temperature gradients, and physical stimuli that guide them to food sources, mates, and suitable habitats. Chemoattraction allows plant-parasitic nematodes to efficiently find host plant roots.
3) Physical factors like temperature, oxygen, water availability, and pore size influence nematode movement rates and distribution in the soil environment. Nematodes concentrate near optimal temperature ranges and in moist
This document discusses the potential benefits of conducting biomedical research under microgravity conditions in space. Microgravity allows scientists to grow better protein crystals and observe cell and tissue cultures in a way that is not possible on Earth. The International Space Station provides an environment for experiments in areas like cancer research, stem cells, tissue engineering and infectious diseases. Previous space-based experiments have yielded new insights, such as discovering a more effective crystal structure of an interferon drug. Continued microgravity research could advance understanding of human health and lead to new medical treatments and commercial applications.
This document summarizes research on the role of calcium and motility in sperm cell viability and function. It discusses how calcium regulates flagellar movement and capacitation processes like the acrosome reaction. Maintaining low motility through modulating calcium channels may increase sperm cell viability by reducing reactive oxygen species production from flagellar activity. The document also reviews various ion channels involved in calcium signaling in sperm and how environmental factors like temperature can impact sperm cell physiology and preservation.
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How to Make a Field Mandatory in Odoo 17Celine George
In Odoo, making a field required can be done through both Python code and XML views. When you set the required attribute to True in Python code, it makes the field required across all views where it's used. Conversely, when you set the required attribute in XML views, it makes the field required only in the context of that particular view.
This document provides an overview of wound healing, its functions, stages, mechanisms, factors affecting it, and complications.
A wound is a break in the integrity of the skin or tissues, which may be associated with disruption of the structure and function.
Healing is the body’s response to injury in an attempt to restore normal structure and functions.
Healing can occur in two ways: Regeneration and Repair
There are 4 phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. This document also describes the mechanism of wound healing. Factors that affect healing include infection, uncontrolled diabetes, poor nutrition, age, anemia, the presence of foreign bodies, etc.
Complications of wound healing like infection, hyperpigmentation of scar, contractures, and keloid formation.
Chapter wise All Notes of First year Basic Civil Engineering.pptxDenish Jangid
Chapter wise All Notes of First year Basic Civil Engineering
Syllabus
Chapter-1
Introduction to objective, scope and outcome the subject
Chapter 2
Introduction: Scope and Specialization of Civil Engineering, Role of civil Engineer in Society, Impact of infrastructural development on economy of country.
Chapter 3
Surveying: Object Principles & Types of Surveying; Site Plans, Plans & Maps; Scales & Unit of different Measurements.
Linear Measurements: Instruments used. Linear Measurement by Tape, Ranging out Survey Lines and overcoming Obstructions; Measurements on sloping ground; Tape corrections, conventional symbols. Angular Measurements: Instruments used; Introduction to Compass Surveying, Bearings and Longitude & Latitude of a Line, Introduction to total station.
Levelling: Instrument used Object of levelling, Methods of levelling in brief, and Contour maps.
Chapter 4
Buildings: Selection of site for Buildings, Layout of Building Plan, Types of buildings, Plinth area, carpet area, floor space index, Introduction to building byelaws, concept of sun light & ventilation. Components of Buildings & their functions, Basic concept of R.C.C., Introduction to types of foundation
Chapter 5
Transportation: Introduction to Transportation Engineering; Traffic and Road Safety: Types and Characteristics of Various Modes of Transportation; Various Road Traffic Signs, Causes of Accidents and Road Safety Measures.
Chapter 6
Environmental Engineering: Environmental Pollution, Environmental Acts and Regulations, Functional Concepts of Ecology, Basics of Species, Biodiversity, Ecosystem, Hydrological Cycle; Chemical Cycles: Carbon, Nitrogen & Phosphorus; Energy Flow in Ecosystems.
Water Pollution: Water Quality standards, Introduction to Treatment & Disposal of Waste Water. Reuse and Saving of Water, Rain Water Harvesting. Solid Waste Management: Classification of Solid Waste, Collection, Transportation and Disposal of Solid. Recycling of Solid Waste: Energy Recovery, Sanitary Landfill, On-Site Sanitation. Air & Noise Pollution: Primary and Secondary air pollutants, Harmful effects of Air Pollution, Control of Air Pollution. . Noise Pollution Harmful Effects of noise pollution, control of noise pollution, Global warming & Climate Change, Ozone depletion, Greenhouse effect
Text Books:
1. Palancharmy, Basic Civil Engineering, McGraw Hill publishers.
2. Satheesh Gopi, Basic Civil Engineering, Pearson Publishers.
3. Ketki Rangwala Dalal, Essentials of Civil Engineering, Charotar Publishing House.
4. BCP, Surveying volume 1
Temple of Asclepius in Thrace. Excavation resultsKrassimira Luka
The temple and the sanctuary around were dedicated to Asklepios Zmidrenus. This name has been known since 1875 when an inscription dedicated to him was discovered in Rome. The inscription is dated in 227 AD and was left by soldiers originating from the city of Philippopolis (modern Plovdiv).
Main Java[All of the Base Concepts}.docxadhitya5119
This is part 1 of my Java Learning Journey. This Contains Custom methods, classes, constructors, packages, multithreading , try- catch block, finally block and more.
9. Gravity sensing in Arabidopsis
Dynamics of Amyloplasts and AFs in Wild Type and fiz1/+ Endodermal Cells.
Linear actin cable and amyloplast. The arrow indicates an amyloplast that has moved a long
distance along a linear actin cable.
19. Effects on Secondary Metabolism
Increased isoflavonoids in soy bean 6-
day exposure to micro gravity.
β -carotene, neoxanthin, violaxanthin,
and lutein in aquatic plant
Ceratophyllum (9-d)
75 % more glucosinolate in Brassica
rappa.