The Phytobiomes Initiative proposes a systems-level approach to studying the entire microbial community associated with plants, including bacteria, viruses, and eukaryotes in the rhizosphere, phyllosphere, and within plants. Recent advances in metagenomic technologies now allow comprehensive analysis of both culturable and non-culturable microbes. Two recent studies using these methods revealed that root microbial communities are non-random and depend on host genotype and environment. The initiative aims to establish a foundation for understanding how phytobiomes influence plant health and productivity, with the goal of developing strategies to improve crop yields, reduce disease and environmental impacts, and enhance food safety and security.