This document provides information about niosomes, which are non-ionic surfactant based vesicles that can encapsulate hydrophilic or lipophilic drugs. It discusses the structure of niosomes including the non-ionic surfactants, cholesterol, and charged molecules used. Various methods for preparing niosomes are described along with evaluating parameters and techniques. The advantages of niosomes for targeted drug delivery are highlighted, while some disadvantages like limited shelf life are also noted.
Niosome is a novel drug delivery system used for drug delivery to special area Or we can say it is used for targated drug delivery system.
Niosome are superior carrier than liposome as they are made up of non ionic surfectants. Niosome are more stable and more effective carrier than liposome and specialy ideal for hydrophobic and peptide drug
Niosome is a novel drug delivery system used for drug delivery to special area Or we can say it is used for targated drug delivery system.
Niosome are superior carrier than liposome as they are made up of non ionic surfectants. Niosome are more stable and more effective carrier than liposome and specialy ideal for hydrophobic and peptide drug
Niosomes, Aquasomes, Phytosomes,Electrosomes Molecular pharmaceutics (MPH 201T) PRESENTATION BY- NARAYAN R KOTE M PHARM [PHARMACEUTICS] ROLL NO. 8 GUIDANCE BY :- Dr . TIWARI S. S
CONTENTS
NIOSOMES
AQUASOMES
PHYTOSOMES
ELECTROSOMES
NIOSOMES
Niosomes are a novel drug delivery system, in which the medication is encapsulated in a vesicle. The vesicle is composed of a bilayer of non-ionic surface active agents and hence the name niosomes.
The niosomes are very small, and microscopic in size.
Their size lies in the nanometric scale. Although structurally similar to liposomes, they offer several advantages over them.
Niosomes have recently been shown to greatly increase transdermal drug delivery and also can be used in targeted drug delivery, and thus increased study in these structures can provide new methods for drug delivery.
STRUCTURE OF NIOSOMES
Structurally, niosomes are similar to liposomes, in that they are also made up of a bilayer.
However, the bilayer in the case of niosomes is made up of non-ionic surface active agents rather than phospholipids as seen in the case of liposomes.
Most surface active agents when immersed in water yield micellar structures however some surfactants can yield bilayer vesicles which are niosomes.
STRUCTURE OF NIOSOMES
Structurally, niosomes are similar to liposomes, in that they are also made up of a bilayer.
However, the bilayer in the case of niosomes is made up of non-ionic surface active agents rather than phospholipids as seen in the case of liposomes.
Most surface active agents when immersed in water yield micellar structures however some surfactants can yield bilayer vesicles which are niosomes.
APPLICATION OF NIOSOMES
Drug Targetting
One of the most useful aspects of niosomes is their ability to target drugs.
Niosomes can be used to target drugs to the reticuloendothelial system.It can be achieved by coating with polymer e.g. PEG.
In Diagnosis
Niosomes have also been used as carriers for iobitridol, a diagnostic agent used for X-ray imaging.
Anti-neoplastic Treatment
Most antineoplastic drugs cause severe side effects.
Niosomes can alter the metabolism; prolong circulation and half life of the drug, thus decreasing the side effects of the drugs.
Niosomes, is decreased rate of proliferation of tumor and higher plasma levels accompanied by slower elimination.Leishmaniasis :-
Leishmaniasis is a disease in which a parasite of the genus Leishmania invades the cells of the liver and spleen.
Use of niosomes in tests conducted showed that it was possible to administer higher levels of the drug without the triggering of the side effects, and thus allowed greater efficacy in treatment.
Delivery of Peptide Drugs:-
Oral peptide drug delivery has long been faced with a challenge of bypassing the enzymes which would breakdown the peptide.
Use of niosomes to successfully protect the peptides from gastrointestinal peptide breakdown is being investigated.
In an in-vitro study conducted by ODDS.
Niosomes is under the Novel drug delivery system. In which the drug are enclosed in the bilayer vesicle which is made up of the phospholipid. Niosomes are the similar to the liposomes both are made up of the bilayer of phospholipid. But in niosomes several advantages of over the liposomes.
NIOSOMES , GENERAL CHARACTERISTICS OF NIOSOME , TYPES OF NIOSOMES , OTHERS TYPES OF NIOSOMES , NIOSOMES VS LIPOSOMES , COMPONENTS OF NIOSOMES , Non-ionic surfactant , Cholesterol , Charge inducing molecule , METHOD OF PREPARATION , preparation of small unilamellar vesicles , Sonication , Micro fluidization , preparation of large unilamellar vesicles , Reverse Phase Evaporation , Ether Injection , preparation of Multilamellar vesicles , Hand shaking method , Trans membrane pH gradient drug uptake process (remote loading) , Miscellaneous method :Multiple membrane extrusion method , The “Bubble” Method , Formation of Niosomes From Proniosomes , SEPARATION OF UNENTRAPPED DRUGS , Gel Filtration , Dialysis , Centrifugation , FACTORS AFFECTING THE PHYSICOCHEMICAL PROPERTIES OF NIOSOMES , Membrane Additives , Temperature of Hydration , PROPERTIES OF DRUGS , AMOUNT AND TYPE OF SURFACTANT
Structure of Surfactants , Resistance to Osmotic Stress , Characterization of niosomes ,Therapeutic applications of Niosomes , For Controlled Release of Drugs , To Improve the Stability and Physical Properties of the Drugs , For Targeting and Retention of Drug in Blood Circulation , Proniosomes , Aspasomes , Vesicles in Water and Oil System (v/w/o) ,Bola - niosomes , Discomes , Deformable niosomes or elastic niosomes , According to the nature of lamellarity ,Small Unilamellar vesicles (SUV) 25 – 500 nm in size.,Large Unilamellar vesicles (LUV) 0.1 – 1μm in size , Multilamellar vesicles (MLV) 1-5 μm in size , According to the size:Small Niosomes (100 nm – 200 nm) , Large Niosomes (800 nm – 900 nm),Big Niosomes (2 μm – 4 μm)
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Niosomes, Aquasomes, Phytosomes,Electrosomes Molecular pharmaceutics (MPH 201T) PRESENTATION BY- NARAYAN R KOTE M PHARM [PHARMACEUTICS] ROLL NO. 8 GUIDANCE BY :- Dr . TIWARI S. S
CONTENTS
NIOSOMES
AQUASOMES
PHYTOSOMES
ELECTROSOMES
NIOSOMES
Niosomes are a novel drug delivery system, in which the medication is encapsulated in a vesicle. The vesicle is composed of a bilayer of non-ionic surface active agents and hence the name niosomes.
The niosomes are very small, and microscopic in size.
Their size lies in the nanometric scale. Although structurally similar to liposomes, they offer several advantages over them.
Niosomes have recently been shown to greatly increase transdermal drug delivery and also can be used in targeted drug delivery, and thus increased study in these structures can provide new methods for drug delivery.
STRUCTURE OF NIOSOMES
Structurally, niosomes are similar to liposomes, in that they are also made up of a bilayer.
However, the bilayer in the case of niosomes is made up of non-ionic surface active agents rather than phospholipids as seen in the case of liposomes.
Most surface active agents when immersed in water yield micellar structures however some surfactants can yield bilayer vesicles which are niosomes.
STRUCTURE OF NIOSOMES
Structurally, niosomes are similar to liposomes, in that they are also made up of a bilayer.
However, the bilayer in the case of niosomes is made up of non-ionic surface active agents rather than phospholipids as seen in the case of liposomes.
Most surface active agents when immersed in water yield micellar structures however some surfactants can yield bilayer vesicles which are niosomes.
APPLICATION OF NIOSOMES
Drug Targetting
One of the most useful aspects of niosomes is their ability to target drugs.
Niosomes can be used to target drugs to the reticuloendothelial system.It can be achieved by coating with polymer e.g. PEG.
In Diagnosis
Niosomes have also been used as carriers for iobitridol, a diagnostic agent used for X-ray imaging.
Anti-neoplastic Treatment
Most antineoplastic drugs cause severe side effects.
Niosomes can alter the metabolism; prolong circulation and half life of the drug, thus decreasing the side effects of the drugs.
Niosomes, is decreased rate of proliferation of tumor and higher plasma levels accompanied by slower elimination.Leishmaniasis :-
Leishmaniasis is a disease in which a parasite of the genus Leishmania invades the cells of the liver and spleen.
Use of niosomes in tests conducted showed that it was possible to administer higher levels of the drug without the triggering of the side effects, and thus allowed greater efficacy in treatment.
Delivery of Peptide Drugs:-
Oral peptide drug delivery has long been faced with a challenge of bypassing the enzymes which would breakdown the peptide.
Use of niosomes to successfully protect the peptides from gastrointestinal peptide breakdown is being investigated.
In an in-vitro study conducted by ODDS.
Niosomes is under the Novel drug delivery system. In which the drug are enclosed in the bilayer vesicle which is made up of the phospholipid. Niosomes are the similar to the liposomes both are made up of the bilayer of phospholipid. But in niosomes several advantages of over the liposomes.
NIOSOMES , GENERAL CHARACTERISTICS OF NIOSOME , TYPES OF NIOSOMES , OTHERS TYPES OF NIOSOMES , NIOSOMES VS LIPOSOMES , COMPONENTS OF NIOSOMES , Non-ionic surfactant , Cholesterol , Charge inducing molecule , METHOD OF PREPARATION , preparation of small unilamellar vesicles , Sonication , Micro fluidization , preparation of large unilamellar vesicles , Reverse Phase Evaporation , Ether Injection , preparation of Multilamellar vesicles , Hand shaking method , Trans membrane pH gradient drug uptake process (remote loading) , Miscellaneous method :Multiple membrane extrusion method , The “Bubble” Method , Formation of Niosomes From Proniosomes , SEPARATION OF UNENTRAPPED DRUGS , Gel Filtration , Dialysis , Centrifugation , FACTORS AFFECTING THE PHYSICOCHEMICAL PROPERTIES OF NIOSOMES , Membrane Additives , Temperature of Hydration , PROPERTIES OF DRUGS , AMOUNT AND TYPE OF SURFACTANT
Structure of Surfactants , Resistance to Osmotic Stress , Characterization of niosomes ,Therapeutic applications of Niosomes , For Controlled Release of Drugs , To Improve the Stability and Physical Properties of the Drugs , For Targeting and Retention of Drug in Blood Circulation , Proniosomes , Aspasomes , Vesicles in Water and Oil System (v/w/o) ,Bola - niosomes , Discomes , Deformable niosomes or elastic niosomes , According to the nature of lamellarity ,Small Unilamellar vesicles (SUV) 25 – 500 nm in size.,Large Unilamellar vesicles (LUV) 0.1 – 1μm in size , Multilamellar vesicles (MLV) 1-5 μm in size , According to the size:Small Niosomes (100 nm – 200 nm) , Large Niosomes (800 nm – 900 nm),Big Niosomes (2 μm – 4 μm)
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Surat @ℂall @Girls ꧁❤8527049040❤꧂@ℂall @Girls Service Vip Top Model Safe
niosome anshul vishwakarma .pptx
1. Department of pharmaceutical sciences
Dr. Hari Singh Gour Vishwavidyalaya
Sagar, (M.P.)
(A CENTRAL UNIVERSITY)
PRESENTED BY:-
Anshul Vishwakarma
Y21254007
NIOSoMOES
3. DEFINITION
Niosomes are non - ionic surfactant based unilamellar or multilamellar bilayer vesicles .
These are formed upon hydration of non ionic surfactants with or without incorporation of
cholesterol.
The niosomes are very small , and microscopic in size . Their size lies in the nanometric
scale.
Niosomes are a novel drug delivery system , in which the medication is encapsulated in a
vesicle .
Both hydrophilic & lipophilic drugs , can be entrapped either in the aqueous layer or in lipid
layer.
4. CLASSIFICATION OF NIOSOMES
• The niosomes are classified as a function of the number of bilayer (e.g. MLV, SUV) or as a function of size. (e.g.
LUV, SUV) or as a function of the method of preparation
• The various types of niosomes are described below:
A. Multi lamellar vesicles (MLV, size ≥ 0.05 µm)
B. Large unilamellar vesicles (LUV, size ≥ 0.10 µm)
C. Small unilamellar vesicles (SUV, size = 0.025-0.05 µm)
5. STRUCTURE OF NIOSOMES
• Niosomes are microscopic lamellar structures
Basic structural components are
I. Non ionic surfactant
II. cholesterol
III. Charge inducing molecule
6. NON-IONIC SURFACTANTS
• Selection of surfactant should be done on the basis of HLB value.
• As hydrophilic lipophilic balance (HLB) is a good indicator of the vesicle forming ability of any surfactant,
HLB number in between 4 and 8 is found to be compatible with vesicle formation.
• Alkyl ethers: some surfactants for the preparation of niosomes containing drugs/chemicals as:
1) surfactant-i (mol.Wt.473) is C16 mono alkyl glycerol ether with average of three glycerol units.
2) surfactant-ii (mol.Wt.972) is diglycerol ether with average of the seven glycerol units.
3) surfactant iii (mol.Wt.393) is ester linked surfactant.
• Alkyl esters
• Alkyl amides
• Fatty acid and amino acid compounds
7. CHOLESTEROL
• Steroids are important components of the cell membrane and their presence in membrane affect the bilayer
fluidity and permeability. Cholesterol is a steroid derivative, which is mainly used for the formulation of
niosomes.
• Although it may not show any role in the formation of bilayer, its importance in formation of niosomes and
manipulation of layer characteristics can not be discarded. In general, incorporation of cholesterol affect
properties of niosomes like membrane permeability, rigidity, encapsulation efficiency, ease of rehydration of
freeze dried niosomes and their toxicity.
• As a result of this, the niosome become less leaky in nature.
8. CHARGEDMOLECULE:
• Some charged molecules are added to niosomes to increase stability of niosomes by
electrostatic repulsion which prevents coalescence.
• The negatively charged molecules used are diacetyl phosphate (dcp) and phosphotidic acid.
Similarly, stearyl amine (STR) and stearyl pyridinium chloride are the well known
positively charged molecules used in niosomal preparations.
• These charged molecules are used mainly to prevent aggregation of niosomes.
10. METHODOF PREPARATION
1.Ether injection (LUV) based on the vesicle size, niosomes can be divided into three groups.
2. Hand shaking method (MLV) these are small unilamellar vesicles (SUV, size=0.025-0.05 μm),
3. The “bubble” method multilamellar vesicles (MLV, size=>0.05 μm), and large
4. Reverse phase evaporation (LUV) unilamellar vesicles (LUV, size=>0.10 μm).
5. Sonication (SUV)
6. Multiple membrane extrusion method
7. Trans membrane ph gradient drug uptake process (remote loading) (MLV)
8. Microfluidization method (SUV)
9. Formation of niosomes from proniosomes
11. COMMONSTAGESOF ALL METHODSOF PREPARATIONOF NIOSOMES
Cholesterol + non ionic surfactant
Dissolve in organic solvent
Solution in organic solvent
Drying
Thin film
Dispersion (hydration)
Niosome suspension
12. • ETHER INJECTIONMETHOD
Niosomes by slowly introduce in a solution of
surfactant dissolve in diethyl ether into warm water
maintain at 60 C
Mixture in ether is injected through 14-gauge needle
into an aqueous solution of material
Vaporization of ether leads to the formation of the
single layer vesicles
Diameter of the vesicle range from 50 to 1000 nm
depends upon the conditions use
13. HANDSHAKINGMETHOD
The mixing ingredients - surfactant and cholesterol and charge
induce
Dissolves in a volatile organic solvent (chloroform, diethyl ether
or methanol) in a round bottom flask
By using a rotary evaporator organic solvent is evaporated at
room temperature 20°C
Forming a thin layer of solid mixture
The dry surfactant film can be re-hydrated with an aqueous phase
at 0-60°C with gentle agitation
Formation of niosomes
14. • THE “BUBBLE” METHOD
Bubbling unit involves round-bottomed flask with three
neck position in water bath to control the temperature
Water-cool reflux is positioned in the first neck and
thermometer is positioned in the second neck and nitrogen
supply through the third neck
Cholesterol and surfactant are dispersed in the buffer (ph
7.4) at 70°C dispersion mixing for 15 seconds with high
shear homogenizer
“Bubbled” at 70°c using nitrogen gas
15. • REVERSEPHASEEVAPORATION
Surfactant:cholesterol(1:1) in ether or chloroform
Drug in aqueous phase
Sonicated at 4-5˚c
Add pHs & sonicated
organic phase removed at 40˚c under reduced pressure
Viscous niosome suspension diluted with pHs
Heated on a water bath at 60˚c for 10 min
Niosomes
16. • SONICATION
Mixture of drug solution in the buffer, surfactant and cholesterol
Sonicated with a titanium probe sonicator at 60°c for 3 minutes to yield niosomes
17. • MULTIPLEMEMBRANEEXTRUSIONMETHOD
• Mixture of surfactant, cholesterol and dicetyl
phosphate in chloroform forms thin film by
rotary evaporator.
• The film hydrates with aqueous drug
polycarbonate membranes.
• Solution and resultant suspension extrude
through polycarbonate membrane and placed in
series for up to 8 passages. It is a good method
for niosome size control
18. • TRANSMEMBRANEPHGRADIENTDRUGUPTAKEPROCESS
In remote loading process surfactants and cholesterol are dissolved in organic solvent
(chloroform)
solvent evaporates under reduced pressure to get a thin film on the wall of the round
bottom flask
film hydrates with 300 mm citric acid (pH4.0) by vortex mixing
multilamellar vesicles are frozen and thawed 3 times and later sonication
for niosomal suspension, aqueous solution containing 10 mg/ml of drug is added and
vortex
sample pH is raises to 7.0-7.2 with 1M disodium phosphate
the mixture is later heated at 60°C for 10 minutes to yield niosomes
19. • FORMATIONOFNIOSOMESFROMPRONIOSOMES:
• Proniosome is a dry formulation in which each water soluble particle are covered with a thin film of dry
surfactant.
• The niosomes are recognizing by the adding aqueous phase at T > tm with brief agitation.
T is the temperature and tm is the mean phase transition temperature
Carrier + Surfactant = proniosomes,
Proniosomes + Water = Niosomes
20. • SEPARATIONOF UNENTRAPPEDDRUG
The removal of unentrapped solute from the vesicles can be accomplished
by various techniques, which include: -
1) Dialysis: the aqueous niosomal dispersion is dialyzed in dialysis tubing
against phosphate buffer or normal saline or glucose solution.
2) Gel filtration: the unentrapped drug is removed by gel filtration of
niosomal dispersion through a sephadexg-50 column and elution with
phosphate buffered saline or normal saline.
3) Centrifugation: the niosomal suspension is centrifuged and the
supernatant is separated. The pellet is washed and then resuspended to
obtain a niosomal suspension free from unentrapped drug.
21. • EVALUATIONPARAMETERS
Entrapment efficiency (%EE)
Size, shape and morphology
In vitro release study
Tissue distribution/in vivo study
Stability study
Number of lamellae
Membrane rigidity
Vesicular surface charge
22. • APPLICATIONOF NIOSOME
• Ophthalmic drug delivery
• Localized drug action
• Diagnostic imaging with noisome
• Transdermal delivery of drugs by niosomes
• Niosome as a carrier for hemoglobin
• Targeting of bioactive agent
23. ADVANTAGESOF NIOSOMES
Targeted drug delivery can be achieved using niosomes the drug is delivered directly to the body
part where the therapeutic effect is required
Reduced dose is required to achieve the desired effect
Subsequent decrease in the side effects
The therapeutic efficacy of the drugs is improved by reducing the clearance rate, targeting to the
specific site and by protecting the encapsulated drug
Niosomes are amphiphillic i.E. Both hydrophilic and lipophillic in nature and can accommodate a
large number of drugs with a wide range of solubilities
Improve the oral bioavailability of poorly soluble drugs
Enhance the skin permeability of drugs when applied topically
24. DISADVANTAGESOF NIOSOME
• Aqueous suspension of niosome may exhibit fusion, aggregation leaching or hydrolysis of
entrapped drug, thus limiting the shelf life of niosome dispersion.
• Time consuming
• Requires specialized equipment
• Inefficient drug loading
25. REFERENCES
• Jain N.K.’’Advances controlled and novel drug delivery system’’ Ed.1 CBS Publishers and distributors ,New
Delhi 2000, pg.300-320
• Mithal B. M., A text book of pharmaceutical formulation, ed 6 , Vallabh prakashan , 306-307
• Kaur D , Kumar S , Niosomes: present scenario and future aspects Journal of drug delivery & therapeutics.
2018; 8(5):35-43