Fungi are eukaryotic organisms that were historically classified as plants but are now recognized as a distinct kingdom. They differ from plants in that they lack chlorophyll and their cell walls contain chitin rather than cellulose. Fungi reproduce both sexually through the formation of spores like zygospores or basidiospores, and asexually through budding or the formation of conidia. Major groups of fungi include molds, mushrooms, and yeasts. Molds form branching filaments called hyphae that allow them to absorb nutrients, while mushrooms form visible fruiting bodies above ground. Yeasts are single-celled fungi that reproduce by budding.
This document discusses the morphology, classification, reproduction, and cultivation of fungi. It begins by defining fungi and their key characteristics such as eukaryotic cells with chitin cell walls. It then describes the morphological features of fungi including hyphae, mycelium, and differences between molds, yeasts, and dimorphic fungi. The document outlines methods of fungal classification and discusses life cycles involving asexual reproduction through spores and sexual reproduction. It concludes by covering common media used for fungal cultivation, such as Sabouraud agar.
PHARMACEUTICAL MICROBIOLOGY (BP303T) Unit-III Part-1 Study of morphology, cla...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-IIIPart-1Study of morphology, classification, reproduction/replication and cultivation of fungi, Introduction fungi. Morphological Characteristics of fungi, CLASSIFICATION: Depending on cell morphology, fungi can be divided into 4 classes:
Moulds Yeasts ,Yeast like fungi and
Dimorphic fungi
Depending on their sexual spores formation fungi are divided into 4 classes:
Zygomycetes Ascomycetes
Basidiomycetes Dueteromycetes
Reproduction and sporulation;Vegetative, Asexual
and Sexual
Vegetative reproduction: Fragmentation ,Fission, budding, Sclerotia Rhizomorphs
Asexual reproduction: Zoospores
Sporangiospore, Conidia
Oidia Uredospores ,Basidiospores
Sexual reproduction:Planogametic copulation: Isogamy Heterogamy
Gametangial contact
Gametangial copulation Spermatization Somatogamy CULTIVATION OF FUNGI: Brain Heart Infusion (BHT) agar
Czapek’s agar
Mycobiotic agar Inhibitory mold agar (IMA)
Potato dextrose agar
Sabouraud’s dextrose agar (SDA):
Sabouraud’s heart infusion (SABHI) agar
Potato Flake agar
Potato dextrose-yeast extract agar (PDYA)
. Cornmeal agar
Malt extract agar (MEA)
This presentation includes all the general characteristics of fungi, types, structure of a fungi, classifications, and reproduction. Different types of fungi and its classification, its reproduction are all included.
2. General characteristics of microbes (Microbiology)Jay Khaniya
This document defines and describes various types of microorganisms. It discusses that microorganisms include bacteria, viruses, fungi, parasites and protozoa. Bacteria can be pathogenic or non-pathogenic, and are classified based on shape, staining, metabolism and other characteristics. Viruses are intracellular parasites that infect host cells to replicate. Fungi reproduce asexually through spores or hyphae and can cause infections. Parasites like protozoa and helminths live in or on a host and are pathogenic.
This document provides an introduction to mycology, the study of fungi. It defines fungi as eukaryotic organisms that lack chlorophyll and produce filamentous structures and spores. There are over 100,000 known fungal species, with around 300 pathogenic to humans. Fungi have cell walls containing chitin rather than peptidoglycan and cell membranes with ergosterol rather than cholesterol. Many medically important fungi can exist in both yeast and mold forms depending on temperature (dimorphism). Fungi are classified based on their morphology, life cycle and reproduction into groups such as zygomycetes, ascomycetes, and basidiomycetes.
This document provides an overview of mycology (the study of fungi). It discusses that fungi are eukaryotic organisms that lack chlorophyll and can exist as unicellular or multicellular forms. It describes the cell structure of fungi including their cell walls made of chitin and cell membranes containing ergosterol. It covers the taxonomic classification of fungi into phyla and discusses the structures and reproduction of different types of fungi including yeasts, molds, and thermally dimorphic fungi. It also addresses the laboratory diagnosis of fungal infections through microscopy, staining, culture and the clinical manifestations of different fungal infections.
1) Strategies and structuresIn Protozoans the method of movement .pdfaptelecom16999
1) Strategies and structures:
In Protozoans the method of movement is determined by the type of organism and the
surrounding environment. Protozoans mainly move by cell extension, flagella or pseudopodia
and cilia, the movement as per the presence of structure can be classified as ciliary, flagellar and
amoeboid movement.
Ciliates : Ciliates form the largest group of protozoa. These organisms vary in size and often live
in watery environments, including oceans, marshes, bays and streams. Ciliates move using tiny
cilia, which are hair-like strands that act as sensors and tiny limbs.
Flagella are longer and less numerous that cilia, they use their long tail like flagella to move.
Amoebas : In these two cytoskeleton get polymerized . This creates a vacancy and cytoplasmice
material flow to cover the vacancy created. When amoeba moves cytoplasm moves to the arm
like extension called pseudopodium. This pseudopodium extends and enlarge and hence this
push the animal body towards that respective direction.
2) A) Flagellates can live as single cells, in colonies, or as parasites.
Commonly live in niche\'s of water.
They conduct photosynthesis and have a cell wall.
They contain flagella for propulsion or to create a current to bring in food.
They can inhabit the reproductive tract, alimentary canal, tissue sites and also the blood stream,
lymph vessels and cerebrospinal canal.
B) Pseudopods : Also called as false feet , are projections that can appear and disappear from the
organism\'s body. These are used for movement and to engulf prey and digest them using
enzymes.
C) Apicomplexa : Unicellular and spore forming, most of them possess a unique form of
organelle that comprises a type of plastid called an apicoplast, and an apical complex structure.
They have apicoplast(non photosynthetic plastid) , mitochondria and nuclear genomes.
Lack of cilia, sexual reproduction, use micropores for feeding, and the production of oocysts
containing sporozoites as the infective form.
They have unique gliding capability which enables them to cross through tissues and enter and
leave their host cells. This gliding ability is made possible by the use of adhesions and small
static myosin motors.
3) Key characteristics of fungi :
Fungi are unicellular or multicellular.
Most of the fungi grow as tubular filaments called hyphae
They are haploid.
Fungus are heterotrophs (they can obtain nutrients by absorption) . They absorb food and secrete
enzymes to digest complex molecules
Propogate by spores
Asexual or sexual reproduction
They can be multinucleated
Fungi are achlorophyllous (lack of cholorphyll pigment)
Both Fungi and protists belong to same kingdom but fungi is different from protist, protists are
able to live in an anaerobic environment without oxygen but fungi need aerobic respiration to
survive.
Protists are unicellular but fungi are multicellular. Protists are autotrophic (make their own
energy) and heterotrophic (rely on outside source to get energy), but fungi a.
type of microbes & growth (kabashor).pptxMotazKabashor
This document summarizes different types of microorganisms and their growth mechanisms. It describes bacteria, archaea, algae, fungi, protozoa, viruses, prions and lichens. It notes that bacteria are prokaryotic cells that can be classified by shape and cell wall composition. Archaea differ from bacteria in their cell structure and environments. The document outlines characteristics of algae, fungi and protozoa. It explains that viruses are obligate parasites that cause many diseases. Prions cause neurological diseases by converting normal proteins. Lichens are symbiotic organisms of fungi and microbes. The three mechanisms of microbial growth are binary fission, cell division and budding.
This document discusses the morphology, classification, reproduction, and cultivation of fungi. It begins by defining fungi and their key characteristics such as eukaryotic cells with chitin cell walls. It then describes the morphological features of fungi including hyphae, mycelium, and differences between molds, yeasts, and dimorphic fungi. The document outlines methods of fungal classification and discusses life cycles involving asexual reproduction through spores and sexual reproduction. It concludes by covering common media used for fungal cultivation, such as Sabouraud agar.
PHARMACEUTICAL MICROBIOLOGY (BP303T) Unit-III Part-1 Study of morphology, cla...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-IIIPart-1Study of morphology, classification, reproduction/replication and cultivation of fungi, Introduction fungi. Morphological Characteristics of fungi, CLASSIFICATION: Depending on cell morphology, fungi can be divided into 4 classes:
Moulds Yeasts ,Yeast like fungi and
Dimorphic fungi
Depending on their sexual spores formation fungi are divided into 4 classes:
Zygomycetes Ascomycetes
Basidiomycetes Dueteromycetes
Reproduction and sporulation;Vegetative, Asexual
and Sexual
Vegetative reproduction: Fragmentation ,Fission, budding, Sclerotia Rhizomorphs
Asexual reproduction: Zoospores
Sporangiospore, Conidia
Oidia Uredospores ,Basidiospores
Sexual reproduction:Planogametic copulation: Isogamy Heterogamy
Gametangial contact
Gametangial copulation Spermatization Somatogamy CULTIVATION OF FUNGI: Brain Heart Infusion (BHT) agar
Czapek’s agar
Mycobiotic agar Inhibitory mold agar (IMA)
Potato dextrose agar
Sabouraud’s dextrose agar (SDA):
Sabouraud’s heart infusion (SABHI) agar
Potato Flake agar
Potato dextrose-yeast extract agar (PDYA)
. Cornmeal agar
Malt extract agar (MEA)
This presentation includes all the general characteristics of fungi, types, structure of a fungi, classifications, and reproduction. Different types of fungi and its classification, its reproduction are all included.
2. General characteristics of microbes (Microbiology)Jay Khaniya
This document defines and describes various types of microorganisms. It discusses that microorganisms include bacteria, viruses, fungi, parasites and protozoa. Bacteria can be pathogenic or non-pathogenic, and are classified based on shape, staining, metabolism and other characteristics. Viruses are intracellular parasites that infect host cells to replicate. Fungi reproduce asexually through spores or hyphae and can cause infections. Parasites like protozoa and helminths live in or on a host and are pathogenic.
This document provides an introduction to mycology, the study of fungi. It defines fungi as eukaryotic organisms that lack chlorophyll and produce filamentous structures and spores. There are over 100,000 known fungal species, with around 300 pathogenic to humans. Fungi have cell walls containing chitin rather than peptidoglycan and cell membranes with ergosterol rather than cholesterol. Many medically important fungi can exist in both yeast and mold forms depending on temperature (dimorphism). Fungi are classified based on their morphology, life cycle and reproduction into groups such as zygomycetes, ascomycetes, and basidiomycetes.
This document provides an overview of mycology (the study of fungi). It discusses that fungi are eukaryotic organisms that lack chlorophyll and can exist as unicellular or multicellular forms. It describes the cell structure of fungi including their cell walls made of chitin and cell membranes containing ergosterol. It covers the taxonomic classification of fungi into phyla and discusses the structures and reproduction of different types of fungi including yeasts, molds, and thermally dimorphic fungi. It also addresses the laboratory diagnosis of fungal infections through microscopy, staining, culture and the clinical manifestations of different fungal infections.
1) Strategies and structuresIn Protozoans the method of movement .pdfaptelecom16999
1) Strategies and structures:
In Protozoans the method of movement is determined by the type of organism and the
surrounding environment. Protozoans mainly move by cell extension, flagella or pseudopodia
and cilia, the movement as per the presence of structure can be classified as ciliary, flagellar and
amoeboid movement.
Ciliates : Ciliates form the largest group of protozoa. These organisms vary in size and often live
in watery environments, including oceans, marshes, bays and streams. Ciliates move using tiny
cilia, which are hair-like strands that act as sensors and tiny limbs.
Flagella are longer and less numerous that cilia, they use their long tail like flagella to move.
Amoebas : In these two cytoskeleton get polymerized . This creates a vacancy and cytoplasmice
material flow to cover the vacancy created. When amoeba moves cytoplasm moves to the arm
like extension called pseudopodium. This pseudopodium extends and enlarge and hence this
push the animal body towards that respective direction.
2) A) Flagellates can live as single cells, in colonies, or as parasites.
Commonly live in niche\'s of water.
They conduct photosynthesis and have a cell wall.
They contain flagella for propulsion or to create a current to bring in food.
They can inhabit the reproductive tract, alimentary canal, tissue sites and also the blood stream,
lymph vessels and cerebrospinal canal.
B) Pseudopods : Also called as false feet , are projections that can appear and disappear from the
organism\'s body. These are used for movement and to engulf prey and digest them using
enzymes.
C) Apicomplexa : Unicellular and spore forming, most of them possess a unique form of
organelle that comprises a type of plastid called an apicoplast, and an apical complex structure.
They have apicoplast(non photosynthetic plastid) , mitochondria and nuclear genomes.
Lack of cilia, sexual reproduction, use micropores for feeding, and the production of oocysts
containing sporozoites as the infective form.
They have unique gliding capability which enables them to cross through tissues and enter and
leave their host cells. This gliding ability is made possible by the use of adhesions and small
static myosin motors.
3) Key characteristics of fungi :
Fungi are unicellular or multicellular.
Most of the fungi grow as tubular filaments called hyphae
They are haploid.
Fungus are heterotrophs (they can obtain nutrients by absorption) . They absorb food and secrete
enzymes to digest complex molecules
Propogate by spores
Asexual or sexual reproduction
They can be multinucleated
Fungi are achlorophyllous (lack of cholorphyll pigment)
Both Fungi and protists belong to same kingdom but fungi is different from protist, protists are
able to live in an anaerobic environment without oxygen but fungi need aerobic respiration to
survive.
Protists are unicellular but fungi are multicellular. Protists are autotrophic (make their own
energy) and heterotrophic (rely on outside source to get energy), but fungi a.
type of microbes & growth (kabashor).pptxMotazKabashor
This document summarizes different types of microorganisms and their growth mechanisms. It describes bacteria, archaea, algae, fungi, protozoa, viruses, prions and lichens. It notes that bacteria are prokaryotic cells that can be classified by shape and cell wall composition. Archaea differ from bacteria in their cell structure and environments. The document outlines characteristics of algae, fungi and protozoa. It explains that viruses are obligate parasites that cause many diseases. Prions cause neurological diseases by converting normal proteins. Lichens are symbiotic organisms of fungi and microbes. The three mechanisms of microbial growth are binary fission, cell division and budding.
The document summarizes information about the fungi group Zygomycotina. It is divided into two classes: Zygomycetes and Trichomycetes. Zygomycetes are mostly terrestrial fungi that reproduce sexually through the fusion of opposite hyphae to form spores called zygospores. Rhizopus stolonifer is a common member. Trichomycetes are typically symbiotic fungi living in arthropod guts. The document also provides details about the fungi Mucor and Rhizopus, including their structures, life cycles, and reproduction methods.
Fungi are eukaryotic organisms that include mushrooms, molds, and yeasts. They obtain nutrients by absorbing dissolved molecules through enzymes secreted into their environment. Fungi play important roles in decomposition, industrial processes like brewing, and producing medications. They can also cause diseases in plants, animals, and humans. Fungi reproduce both sexually through spores and asexually through fragmentation.
Morphology, Classification, Cultivation and Reproduction of FungiKrutika Pardeshi
This presentation is Useful for B. Pharmacy SEM III Students to study the Topic Fungi According to PCI Syllabus.
It Consist of Morpholoy of Fungi, Cultivation , Reproduction and Classification of Fungi.
This document discusses fungi, including their key features, structures, characteristics and classification. Some of the main points covered include:
- Fungi are eukaryotic organisms that can be unicellular or filamentous, and include mushrooms, molds and yeasts. They have cell walls containing chitin.
- Fungi reproduce both sexually through spores or asexually through budding or fragmentation. They lack chlorophyll and obtain nutrients by absorbing organic matter.
- Fungi are classified based on their mode of nutrition (saprophytic, parasitic, symbiotic) and spore formation (zygomycetes, ascomycetes, basidiomycetes
Introduction to fungus of all kinds.pptxchandanabinu
This document provides an introduction to fungi. It notes that fungi have between 80,000-1.2 million described species, and their vegetative body is made of filaments called hyphae that form a network known as mycelium. Fungi reproduce both sexually and asexually through spores. The cell wall is a key structure for fungi and is composed mainly of chitin and glucans. It provides structure and protects the cell.
Fungi are eukaryotic organisms that lack chlorophyll and have a cell structure consisting of thread-like hyphae that form a mycelium. They can reproduce both sexually through spores and asexually through budding or fission. Fungi encompass a wide range of sizes and forms, from microscopic yeasts to large mushrooms.
Fungi are eukaryotic organisms that can exist as unicellular yeasts or multicellular molds and mushrooms. They have rigid cell walls made of chitin and reproduce both sexually through spores and asexually through budding or fragmentation. Yeasts are unicellular while molds are filamentous and made up of branching hyphae. Fungi play important roles in industries like food production as well as in biodegradation and antibiotic production. Medically important fungi are classified into four divisions: Ascomycetes, Basidiomycetes, Zygomycetes, and Deuteromycetes.
Fungi have several distinguishing morphological features:
1. They have cell walls containing chitin and lack peptidoglycan.
2. They can exist in both unicellular and multicellular forms, dividing asexually or sexually.
3. They are classified based on their structures - yeasts are unicellular, molds form branching hyphae and mycelium, and dimorphic fungi switch between yeast and mold forms based on temperature.
Fungi have several distinguishing morphological features:
1. They have cell walls containing chitin and lack peptidoglycan.
2. They can exist in both unicellular and multicellular forms, dividing asexually or sexually.
3. They are classified based on their structures - yeasts are unicellular, molds form branching hyphae and mycelium, and dimorphic fungi switch between yeast and mold forms based on temperature.
This document provides information about the basic structure and types of eukaryotic cells and fungi. It describes the key organelles in eukaryotic cells like the nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus, lysosomes, cytoskeleton, vacuoles, and plasma membrane. It also discusses the morphology, reproduction, and classification of fungi, including different types of hyphae, spores, and the five main classes of fungi.
This document provides an introduction to the topic of mycology. It discusses the key characteristics of fungi, including their eukaryotic nature and cell wall composition of chitin and glucan. Fungi can exist in either yeast or mold forms and many medically important fungi are dimorphic, being able to live in both forms. The document outlines different types of fungal infections and methods for diagnosis, including microscopic examination, serology, culture and nucleic acid probes.
Microbes, Man and Environment (fungal replication) .pptxMidhatSarfraz
Fungi can reproduce through three main methods: vegetative reproduction which involves fragmentation or budding without spores; asexual reproduction through mitospores like sporangiospores, zoospores, or conidiospores; and sexual reproduction through the fusion of gametes to form ascospores, basidiospores, or zygospores. Fungal reproduction allows them to disperse and form new individuals under varying environmental conditions through both spore-based and non-spore based methods.
This document provides an introduction to medical bacteriology. It defines key terms and describes the structure, growth, and types of bacteria. It discusses that bacteria are single-celled microorganisms that use binary fission to rapidly reproduce. Bacteria have a cell membrane, DNA, cytoplasm, and often a cell wall, flagella, pili, and capsule. Some bacteria are pathogens that can cause human disease, while others are harmless or even beneficial to humans. Disinfection and sterilization are used to eliminate bacteria from surfaces and equipment.
Mycology is the study of fungi. Key developments in the field include the 19th century observation that fungi can infect plants and animals, and the 1910 publication of a comprehensive book on dermatophytic fungi. Fungi play both beneficial and harmful roles. They are important for decomposition, producing antibiotics and enzymes, yet can also cause diseases in animals, humans, and plants or spoil food and materials. Fungi have eukaryotic cells with rigid cell walls and exist primarily in filamentous or unicellular forms. Major groups include molds, yeasts, and dimorphic fungi.
Different modes of reproduction of fungi and reproductive structure and nutri...hassabbinahmed
The document discusses different modes of reproduction in fungi including vegetative, asexual, and sexual reproduction. Vegetative reproduction occurs through fission, budding, fragmentation, or sclerotia formation. Asexual reproduction involves the formation of spores such as sporangiospores, oidia, chlamydospores, or conidia. Sexual reproduction involves plasmogamy, karyogamy, and meiosis, resulting in spores like ascospores or basidiospores. Fungi obtain nutrition through saprotrophic, parasitic, or symbiotic means.
Mycology is the branch of biology concerned with the study of fungi.
The word 'myco' is derived from the Greek word mýkēs meaning “mushroom, fungus”.
Heinrich Anton de Bary is the father of Mycology.
Fungi are eukaryotic organisms that include such as yeasts, moulds and mushrooms. These organisms are classified under kingdom fungi.
Fungi are diverse and widespread.
This document provides an overview of microorganisms and bacteria. It discusses that microorganisms are unicellular or multicellular organisms that include bacteria, fungi, algae, protozoa, and viruses. Bacteria are specifically unicellular prokaryotic organisms that lack membrane-bound organelles. The document describes bacterial cell structure both inside and outside the cell wall, including shapes, flagella, pili, capsules, cell membrane, cytoplasm, nucleoid, plasmids, and ribosomes. It also discusses endospore formation in certain bacteria.
This document provides information on various kingdoms and types of organisms. It discusses key characteristics of Eubacteria, Protista, Fungi, Plantae, and Animalia kingdoms. Within these kingdoms, it describes important groups like cyanobacteria, mycoplasma, chrysophytes, dinoflagellates, euglenoids, slime moulds, protozoans, ascomycetes, basidiomycetes, and deuteromycetes. It also summarizes generalized life cycles of fungi and alternation of generations in plants.
The document discusses fungi and mycology. It defines fungi and describes their key characteristics, including that they are eukaryotic, lack chlorophyll, obtain nutrients through absorption, and have cell walls containing chitin. Fungi can be saprophytes or parasites and reproduce both sexually and asexually through spores. The document outlines fungal cell structure and life cycles. It also describes the different types of fungi based on morphology and sexual reproduction, including yeasts, molds, dimorphic fungi, zygomycetes, ascomycetes, and basidiomycetes.
HIV attacks and weakens the immune system by destroying CD4+ T cells. It progresses through three stages: acute infection, clinical latency, and AIDS. HIV is transmitted through bodily fluids and can be prevented through condom use, PrEP, and harm reduction programs. Diagnosis involves antibody and viral load tests. Treatment is lifelong antiretroviral therapy which suppresses the virus but can cause side effects. Pharmacists play a role by dispensing ART, preventing interactions, and educating patients. Despite progress, efforts are still needed to develop more effective prevention and treatment options like a vaccine in order to end the global HIV epidemic.
Cellulose is a polysaccharide composed of glucose units linked together by beta-1,4-glycosidic bonds. It is the main component of plant cell walls and is produced by plants and some bacteria through biosynthesis. In plants, cellulose synthesis occurs on plasma membrane-bound rosette terminal complexes that polymerize glucose residues from UDP-glucose to form cellulose chains. These chains then assemble into crystalline microfibrils in the cell wall. Cellulose is widely used to produce derivatives like cellulose esters and rayon. Rayon is a regenerated cellulose fiber produced through a chemical process involving wood pulp. It has the same molecular structure as cellulose. Catgut is prepared from the natural
The document summarizes information about the fungi group Zygomycotina. It is divided into two classes: Zygomycetes and Trichomycetes. Zygomycetes are mostly terrestrial fungi that reproduce sexually through the fusion of opposite hyphae to form spores called zygospores. Rhizopus stolonifer is a common member. Trichomycetes are typically symbiotic fungi living in arthropod guts. The document also provides details about the fungi Mucor and Rhizopus, including their structures, life cycles, and reproduction methods.
Fungi are eukaryotic organisms that include mushrooms, molds, and yeasts. They obtain nutrients by absorbing dissolved molecules through enzymes secreted into their environment. Fungi play important roles in decomposition, industrial processes like brewing, and producing medications. They can also cause diseases in plants, animals, and humans. Fungi reproduce both sexually through spores and asexually through fragmentation.
Morphology, Classification, Cultivation and Reproduction of FungiKrutika Pardeshi
This presentation is Useful for B. Pharmacy SEM III Students to study the Topic Fungi According to PCI Syllabus.
It Consist of Morpholoy of Fungi, Cultivation , Reproduction and Classification of Fungi.
This document discusses fungi, including their key features, structures, characteristics and classification. Some of the main points covered include:
- Fungi are eukaryotic organisms that can be unicellular or filamentous, and include mushrooms, molds and yeasts. They have cell walls containing chitin.
- Fungi reproduce both sexually through spores or asexually through budding or fragmentation. They lack chlorophyll and obtain nutrients by absorbing organic matter.
- Fungi are classified based on their mode of nutrition (saprophytic, parasitic, symbiotic) and spore formation (zygomycetes, ascomycetes, basidiomycetes
Introduction to fungus of all kinds.pptxchandanabinu
This document provides an introduction to fungi. It notes that fungi have between 80,000-1.2 million described species, and their vegetative body is made of filaments called hyphae that form a network known as mycelium. Fungi reproduce both sexually and asexually through spores. The cell wall is a key structure for fungi and is composed mainly of chitin and glucans. It provides structure and protects the cell.
Fungi are eukaryotic organisms that lack chlorophyll and have a cell structure consisting of thread-like hyphae that form a mycelium. They can reproduce both sexually through spores and asexually through budding or fission. Fungi encompass a wide range of sizes and forms, from microscopic yeasts to large mushrooms.
Fungi are eukaryotic organisms that can exist as unicellular yeasts or multicellular molds and mushrooms. They have rigid cell walls made of chitin and reproduce both sexually through spores and asexually through budding or fragmentation. Yeasts are unicellular while molds are filamentous and made up of branching hyphae. Fungi play important roles in industries like food production as well as in biodegradation and antibiotic production. Medically important fungi are classified into four divisions: Ascomycetes, Basidiomycetes, Zygomycetes, and Deuteromycetes.
Fungi have several distinguishing morphological features:
1. They have cell walls containing chitin and lack peptidoglycan.
2. They can exist in both unicellular and multicellular forms, dividing asexually or sexually.
3. They are classified based on their structures - yeasts are unicellular, molds form branching hyphae and mycelium, and dimorphic fungi switch between yeast and mold forms based on temperature.
Fungi have several distinguishing morphological features:
1. They have cell walls containing chitin and lack peptidoglycan.
2. They can exist in both unicellular and multicellular forms, dividing asexually or sexually.
3. They are classified based on their structures - yeasts are unicellular, molds form branching hyphae and mycelium, and dimorphic fungi switch between yeast and mold forms based on temperature.
This document provides information about the basic structure and types of eukaryotic cells and fungi. It describes the key organelles in eukaryotic cells like the nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus, lysosomes, cytoskeleton, vacuoles, and plasma membrane. It also discusses the morphology, reproduction, and classification of fungi, including different types of hyphae, spores, and the five main classes of fungi.
This document provides an introduction to the topic of mycology. It discusses the key characteristics of fungi, including their eukaryotic nature and cell wall composition of chitin and glucan. Fungi can exist in either yeast or mold forms and many medically important fungi are dimorphic, being able to live in both forms. The document outlines different types of fungal infections and methods for diagnosis, including microscopic examination, serology, culture and nucleic acid probes.
Microbes, Man and Environment (fungal replication) .pptxMidhatSarfraz
Fungi can reproduce through three main methods: vegetative reproduction which involves fragmentation or budding without spores; asexual reproduction through mitospores like sporangiospores, zoospores, or conidiospores; and sexual reproduction through the fusion of gametes to form ascospores, basidiospores, or zygospores. Fungal reproduction allows them to disperse and form new individuals under varying environmental conditions through both spore-based and non-spore based methods.
This document provides an introduction to medical bacteriology. It defines key terms and describes the structure, growth, and types of bacteria. It discusses that bacteria are single-celled microorganisms that use binary fission to rapidly reproduce. Bacteria have a cell membrane, DNA, cytoplasm, and often a cell wall, flagella, pili, and capsule. Some bacteria are pathogens that can cause human disease, while others are harmless or even beneficial to humans. Disinfection and sterilization are used to eliminate bacteria from surfaces and equipment.
Mycology is the study of fungi. Key developments in the field include the 19th century observation that fungi can infect plants and animals, and the 1910 publication of a comprehensive book on dermatophytic fungi. Fungi play both beneficial and harmful roles. They are important for decomposition, producing antibiotics and enzymes, yet can also cause diseases in animals, humans, and plants or spoil food and materials. Fungi have eukaryotic cells with rigid cell walls and exist primarily in filamentous or unicellular forms. Major groups include molds, yeasts, and dimorphic fungi.
Different modes of reproduction of fungi and reproductive structure and nutri...hassabbinahmed
The document discusses different modes of reproduction in fungi including vegetative, asexual, and sexual reproduction. Vegetative reproduction occurs through fission, budding, fragmentation, or sclerotia formation. Asexual reproduction involves the formation of spores such as sporangiospores, oidia, chlamydospores, or conidia. Sexual reproduction involves plasmogamy, karyogamy, and meiosis, resulting in spores like ascospores or basidiospores. Fungi obtain nutrition through saprotrophic, parasitic, or symbiotic means.
Mycology is the branch of biology concerned with the study of fungi.
The word 'myco' is derived from the Greek word mýkēs meaning “mushroom, fungus”.
Heinrich Anton de Bary is the father of Mycology.
Fungi are eukaryotic organisms that include such as yeasts, moulds and mushrooms. These organisms are classified under kingdom fungi.
Fungi are diverse and widespread.
This document provides an overview of microorganisms and bacteria. It discusses that microorganisms are unicellular or multicellular organisms that include bacteria, fungi, algae, protozoa, and viruses. Bacteria are specifically unicellular prokaryotic organisms that lack membrane-bound organelles. The document describes bacterial cell structure both inside and outside the cell wall, including shapes, flagella, pili, capsules, cell membrane, cytoplasm, nucleoid, plasmids, and ribosomes. It also discusses endospore formation in certain bacteria.
This document provides information on various kingdoms and types of organisms. It discusses key characteristics of Eubacteria, Protista, Fungi, Plantae, and Animalia kingdoms. Within these kingdoms, it describes important groups like cyanobacteria, mycoplasma, chrysophytes, dinoflagellates, euglenoids, slime moulds, protozoans, ascomycetes, basidiomycetes, and deuteromycetes. It also summarizes generalized life cycles of fungi and alternation of generations in plants.
The document discusses fungi and mycology. It defines fungi and describes their key characteristics, including that they are eukaryotic, lack chlorophyll, obtain nutrients through absorption, and have cell walls containing chitin. Fungi can be saprophytes or parasites and reproduce both sexually and asexually through spores. The document outlines fungal cell structure and life cycles. It also describes the different types of fungi based on morphology and sexual reproduction, including yeasts, molds, dimorphic fungi, zygomycetes, ascomycetes, and basidiomycetes.
HIV attacks and weakens the immune system by destroying CD4+ T cells. It progresses through three stages: acute infection, clinical latency, and AIDS. HIV is transmitted through bodily fluids and can be prevented through condom use, PrEP, and harm reduction programs. Diagnosis involves antibody and viral load tests. Treatment is lifelong antiretroviral therapy which suppresses the virus but can cause side effects. Pharmacists play a role by dispensing ART, preventing interactions, and educating patients. Despite progress, efforts are still needed to develop more effective prevention and treatment options like a vaccine in order to end the global HIV epidemic.
Cellulose is a polysaccharide composed of glucose units linked together by beta-1,4-glycosidic bonds. It is the main component of plant cell walls and is produced by plants and some bacteria through biosynthesis. In plants, cellulose synthesis occurs on plasma membrane-bound rosette terminal complexes that polymerize glucose residues from UDP-glucose to form cellulose chains. These chains then assemble into crystalline microfibrils in the cell wall. Cellulose is widely used to produce derivatives like cellulose esters and rayon. Rayon is a regenerated cellulose fiber produced through a chemical process involving wood pulp. It has the same molecular structure as cellulose. Catgut is prepared from the natural
Gels are semisolid systems consisting of small particles suspended in a liquid that is given structure through the addition of a gelling agent. This causes a high degree of physical or chemical cross-linking that results in high viscosity and a semi-solid state. Gels can be single or multiphase depending on if the components are uniformly distributed or separated. Common gelling agents include carbomers, cellulose derivatives, and natural gums. Gels are used topically in products like acne treatments, anti-inflammatories, and vaginal medications due to their ability to maintain drug levels at application sites.
Pesticides are chemical agents used to control or eliminate pests. Natural and artificial controls are used to control pests. Natural controls include predators and changing environmental conditions. Artificial controls include mechanical, agricultural, chemical and biological methods. Common types of pesticides include insecticides, herbicides, fungicides and rodenticides. Many plants contain chemical constituents that make them effective pesticides, such as nicotine from tobacco, rotenone from Derris roots, and azadirachtin from neem. These plant-derived pesticides can control insects, fungi, weeds and rodents in a natural way.
Papaveraceae family (poppy capsule + sanguinaria) .pptxAhmadShafiq43
The document summarizes information about two plants from the Papaveraceae family: Sanguinaria canadensis (bloodroot) and Papaver somniferum (opium poppy). It provides details on their botanical names, parts used, sensory characteristics, major chemical constituents, uses, and dosages. Both plants contain alkaloids with medicinal properties. Bloodroot contains sanguinarine and is used as an emetic and expectorant. Opium poppy contains morphine, codeine, and thebaine and its latex is used as an analgesic, sedative, and to treat diarrhea.
Diagnostic tools for disease evaluation.pdfAhmadShafiq43
This document provides information on various diagnostic tools used for disease evaluation. It describes X-ray scanners, magnetic resonance imaging scanners, computerized tomography scanners, positron emission tomography scans, blood analyzers, urine analyzers, blood pressure monitors, spirometers, ophthalmoscopes, ultrasounds, and biopsies. Each tool is used to examine a different part of the body or test a specific bodily function to aid in disease diagnosis.
This document discusses several central nervous system stimulants and hallucinogens. It describes their mechanisms of action, therapeutic uses, and adverse effects. Key stimulants discussed include caffeine, nicotine, cocaine, amphetamines, atomoxetine, methylphenidate, and modafinil. Hallucinogens covered are LSD, PCP, THC, and rimonabant. Therapeutic uses include treatment of ADHD, narcolepsy, and obesity. Adverse effects can include dependence, withdrawal symptoms, anxiety, increased heart rate and blood pressure, psychosis, and seizures.
The digestive system breaks down food, absorbs nutrients, and eliminates waste. It includes the mouth, esophagus, stomach, small intestine, large intestine, liver, gallbladder and pancreas. Food is ingested, propelled through the system, and undergoes both mechanical and chemical digestion. Nutrients are absorbed through the intestinal walls and waste is eliminated as feces. Glands and organs secrete enzymes and hormones to regulate digestion.
This document provides information about the pharmacognostical study of Aloe vera leaves and latex. It discusses the botanical name, common names, geographical source, sensory characteristics, major chemical constituents including anthraquinone glycosides and specific compounds like aloin, uses as a laxative and for skin benefits, dose, safety concerns, interactions, and phytochemical group of Aloe vera. It also provides references for further information.
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...Sérgio Sacani
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
Authoring a personal GPT for your research and practice: How we created the Q...Leonel Morgado
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
The binding of cosmological structures by massless topological defectsSérgio Sacani
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...AbdullaAlAsif1
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...Leonel Morgado
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
The debris of the ‘last major merger’ is dynamically youngSérgio Sacani
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptxMAGOTI ERNEST
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
2. • Fungi are eukaryotic organisms; i.e., their cells contain membrane-
bound organelles and clearly defined nuclei. Historically, fungi were
included in the plant kingdom; however, because fungi
lack chlorophyll and are distinguished by unique structural and
physiological features (i.e., components of the cell wall and cell
membrane), they have been separated from plants.
3. • Because fungi (yeasts and molds) are eukaryotic organisms, whereas
bacteria are prokaryotic, they differ in several fundamental respects
4.
5. Structure
• fungal cell structures are important medically:
• (1) The fungal cell wall consists primarily of chitin (not peptidoglycan as in
bacteria); thus fungi are insensitive to certain antibiotics, such as penicillins
and cephalosporins, that inhibit peptidoglycan synthesis.
• (2) Chitin is a polysaccharide composed of long chains of N-
acetylglucosamine. The fungal cell wall contains other polysaccharides as
well, the most important of which is β-glucan, a long polymer of d-glucose.
The medical importance of β-glucan is that it is the site of action of the
antifungal drug caspofungin.
• (3) The fungal cell membrane contains ergosterol, in contrast to the human
cell membrane, which contains cholesterol.
6.
7. Types of fungi
• Fungi are subdivided on the basis of their life cycles, the presence or
structure of their fruiting body and the arrangement of and type of
spores (reproductive or distributional cells) they produce.
• The three major groups of fungi are:
1. Multicellular filamentous moulds.
2. Macroscopic filamentous fungi that form large fruiting bodies.
Sometimes the group is referred to as ‘mushrooms’, but the
mushroom is just the part of the fungus we see above ground
which is also known as the fruiting body.
3. Single celled microscopic yeasts.
8. Multicellular filamentous moulds
• Moulds are made up of very fine threads (hyphae). Hyphae grow at the tip and
divide repeatedly along their length creating long and branching chains. The
hyphae keep growing and intertwining until they form a network of threads called
a mycelium. Digestive enzymes are secreted from the hyphal tip. These enzymes
break down the organic matter found in the soil into smaller molecules which are
used by the fungus as food.
• Some of the hyphal branches grow into the air and spores form on these aerial
branches. Spores are specialised structures with a protective coat that shields
them from harsh environmental conditions such as drying out and high
temperatures. They are so small that between 500 – 1000 could fit on a pin head.
• Spores are similar to seeds as they enable the fungus to reproduce. Wind, rain or
insects spread spores. They eventually land in new habitats and if conditions are
right, they start to grow and produce new hyphae. As fungi can’t move they use
spores to find a new environment where there are fewer competing organisms.
9. • Molds grow as long filaments (hyphae) and form a mat (mycelium).
Some hyphae form transverse walls (septate hyphae), whereas others
do not (nonseptate hyphae).
• Nonseptate hyphae are multinucleated (coenocytic). The growth of
hyphae occurs by extension of the tip of the hypha, not by cell
division all along the filament.
10.
11.
12. Macroscopic filamentous fungi
• Macroscopic filamentous fungi also grow by producing a mycelium
below ground. They differ from moulds because they produce visible
fruiting bodies (commonly known as mushrooms or toadstools) that
hold the spores. The fruiting body is made up of tightly packed
hyphae which divide to produce the different parts of the fungal
structure, for example the cap and the stem. Gills underneath the cap
are covered with spores and a 10 cm diameter cap can produce up to
100 million spores per hour.
13.
14. Yeasts
• Yeasts are small, lemon-shaped single cells that are about the same
size as red blood cells. They multiply by budding a daughter cell off
from the original parent cell. Scars can be seen on the surface of the
yeast cell where buds have broken off.
• Yeasts such as Saccharomyces play an important role in the
production of bread and in brewing.
• Yeasts are also one of the most widely used model organisms for
genetic studies, for example in cancer research. Other species of yeast
such as Candida are opportunistic pathogens and cause infections in
individuals who do not have a healthy immune system.
15.
16. Yeast cell structure
• Yeast cells possess ultrastructural features typical of other eukaryotic
cells, with the presence of membrane-bound organelles
• Cell wall compose of glycosylated glycoproteins (mannoproteins), two
types of β-glucans, and chitin.
• The surface plasma membrane of yeast is a lipid bilayer, The lipid
components comprise mainly phospholipids and sterol.
• The periplasmic space is a region external to the plasma membrane
that contains proteins unable to permeate the cell wall, such
as invertase and phosphatase.
17. Yeast cell structure
• The yeast cytoplasm is an aqueous, slightly acidic colloidal fluid that
contains proteins, glycogen, other soluble macromolecules and larger
macromolecular entities such as ribosomes, proteasomes, and lipid
particles. Many essential functions for cellular integrity are localized
in the cytoplasm.
• The cytoskeleton of yeast cells comprises microtubules
and microfilaments.
• In the center of the cell or slightly excentrically, the nuclear structure
is located, which is surrounded by a double membrane that separates
the nucleoplasm from the cytoplasm.
18. Yeast cell structure
• The outer nuclear membrane is contiguous with the membrane of
the endoplasmic reticulum.
• The nuclear chromosomes are in the nucleoplasm and packed into a
chromatin structure
• Yeast cell contains different organelles surrounded by individual
membranes, mostly the endoplasmic reticulum, the Golgi apparatus,
and transport vesicles that are necessary for the manufacturing and
trafficking of proteins, vacuoles, mitochondria, and microbodies.
19.
20. • Several medically important fungi are thermally dimorphic (i.e., they form different
structures at different temperatures).
• They exist as molds in the environment at ambient temperature and as yeasts (or other
structures) in human tissues at body temperature.
• A true or primary fungal pathogen is a species that can invade and grow in a healthy,
noncompromised animal host. This behavior is contrary to the metabolism and
adaptation of fungi, most of which are inhibited by the relatively high temperature and
low oxygen tensions of a warm-blooded animal’s body.
• But a small number of fungi have the morphological and physiological adaptations
required to survive and grow in this habitat. By far, their most striking adaptation is a
switch from hyphal cells typical of the mycelial or mold phase to yeast cells typical of the
parasitic phase.
• This biphasic characteristic of the life cycle is termed thermal dimorphism because it is
initiated by changing temperature. In general, these organisms grow as molds at 30°C
and as yeasts at 37°C.
21.
22. Reproduction
• Fungi reproduce sexually and/or asexually. Perfect fungi reproduce
both sexually and asexually, while imperfect fungi reproduce only
asexually (by mitosis).
• In both sexual and asexual reproduction, fungi produce spores that
disperse from the parent organism by either floating on the wind or
hitching a ride on an animal.
• Fungal spores are smaller and lighter than plant seeds. The giant
puffball mushroom bursts open and releases trillions of spores. The
huge number of spores released increases the likelihood of landing in
an environment that will support growth.
23. The release of fungal spores: The (a) giant puff ball mushroom releases (b) a cloud of
spores when it reaches maturity.
24. Sexual reproduction
• Some fungi reproduce sexually by mating and forming sexual spores
• zygospores,
• ascospores,
• Basidiospores
• Zygospores are single large spores with thick walls;
• ascospores are formed in a sac called ascus;
• basidiospores are formed externally on the tip of a pedestal called a
basidium.
• The classification of these fungi is based on their sexual spores. Fungi that
do not form sexual spores are termed “imperfect” and are classified as
fungi imperfecti (only produce asexualy)
25. Sexual reproduction
• Sexual reproduction introduces genetic variation into a population of
fungi. In fungi, sexual reproduction often occurs in response to
adverse environmental conditions.
• Two mating types are produced. When both mating types are present
in the same mycelium, it is called homothallic, or self-fertile.
• Heterothallic mycelia require two different, but compatible, mycelia
to reproduce sexually.
26. Sexual reproduction
• Although there are many variations in fungal sexual reproduction, all
include the following three stages.
• First, during plasmogamy (literally, “marriage or union of cytoplasm”), two
haploid cells fuse, leading to a dikaryotic stage where two haploid nuclei
coexist in a single cell.
• During karyogamy (“nuclear marriage”), the haploid nuclei fuse to form a
diploid zygote nucleus.
• Finally, meiosis takes place in the gametangia (singular, gametangium)
organs, in which gametes of different mating types are generated.
• At this stage, spores are disseminated into the environment.
27. ASEXUAL REPRODUCTION
• Most fungi of medical interest propagate asexually by forming conidia
(asexual spores) from the sides or ends of specialized structures
• Fungi reproduce asexually by fragmentation, budding, or producing spores.
• Fragments of hyphae can grow new colonies.
• Mycelial fragmentation occurs when a fungal mycelium separates into
pieces with each component growing into a separate mycelium.
• Somatic cells in yeast form buds.
• During budding (a type of cytokinesis), a bulge forms on the side of the cell,
the nucleus divides mitotically, and the bud ultimately detaches itself from
the mother cell.
28. ASEXUAL REPRODUCTION
• The most common mode of asexual reproduction is through the
formation of asexual spores, which are produced by one parent only
(through mitosis) and are genetically identical to that parent.
• Spores allow fungi to expand their distribution and colonize new
environments.
• They may be released from the parent thallus, either outside or
within a special reproductive sac called a sporangium.
29.
30. ASEXUAL REPRODUCTION
• Some important conidia are
• (1) arthrospores, 1 which arise by fragmentation of the ends of
hyphae and are the mode of transmission of Coccidioides immitis;
• (2) chlamydospores, which are rounded, thick-walled, and quite
resistant
• (3) blastospores, which are formed by the budding process by which
yeasts reproduce asexually
• (4) sporangiospores, which are formed within a sac (sporangium) on a
stalk by molds
31.
32. Pathogenesis
• The response to infection with many fungi is the formation of granulomas.
• Granulomas are produced in the major systemic fungal diseases (e.g.,
coccidioidomycosis, histoplasmosis, and blastomycosis, as well as several
others).
• The cell-mediated immune response is involved in granuloma formation.
• Acute suppuration, characterized by the presence of neutrophils in the
exudate, also occurs in certain fungal diseases such as aspergillosis and
sporotrichosis.
• Fungi do not have endotoxin in their cell walls and do not produce
bacterial-type exotoxins.
33.
34. Through skin
• Intact skin is an effective host defense against certain fungi (e.g.,
Candida, dermatophytes), but if the skin is damaged, organisms can
become established.
• Fatty acids in the skin inhibit dermatophyte growth, and hormone-
associated skin changes at puberty limit ringworm of the scalp caused
by Trichophyton.
• The normal flora of the skin and mucous membranes suppress fungi.
When the normal flora is inhibited (e.g., by antibiotics), overgrowth of
fungi such as C. albicans can occur
35.
36. Through respiratory tract
• In the respiratory tract, the important host defenses are the mucous
membranes of the nasopharynx, which trap inhaled fungal spores,
and alveolar macrophages.
• Circulating IgG and IgM are produced in response to fungal infection,
but their role in protection from disease is uncertain.
• The cell-mediated immune response is protective; its suppression can
lead to reactivation
37. Clinical significance
• In addition to mycotic infections, there are two other kinds of fungal
disease:
• (1) mycotoxicoses, caused by ingested toxins,
• (2) allergies to fungal spores
• Ingestion of Amanita mushrooms causes liver necrosis due to the
presence of two fungal toxins, amanitin and phalloidin.
• Amanitin inhibits the RNA polymerase that synthesizes cellular mRNA.
• Ingestion of peanuts and grains contaminated with A. flavus causes
liver cancer due to the presence of aflatoxin.
• Inhalation of the spores of Aspergillus fumigatus can cause allergic
bronchopulmonary aspergillosis. This is an IgE mediated immediate
hypersensitivity response
38. Laboratory diagnosis
• There are four approaches to the laboratory diagnosis of fungal
diseases:
• (1) direct microscopic examination
• (2) culture of the organism
• (3) DNA probe tests
• (4) serologic tests
39. direct microscopic examination
• Direct microscopic examination of clinical specimens such as sputum, lung biopsy
material, and skin scrapings depends on finding characteristic asexual spores,
hyphae, or yeasts in the light microscope.
• The specimen is either treated with 10% potassium hydroxide (KOH) to dissolve
tissue material, leaving the alkali-resistant fungi intact, or stained with special
fungal stains.
• Some examples of diagnostically important findings made by direct examination
are
• (1) the spherules of C. immitis
• (2) the wide capsule of Cryptococcus neoformans seen in India ink preparations
of spinal fluid.
• Calcofluor white is a fluorescent dye that binds to fungal cell walls and is useful in
the identification of fungi in tissue specimens.
• Methenamine silver stain is also useful in the microscopic diagnosis of fungi in
tissue.
40.
41. culture of the organism
• Fungi are frequently cultured on Sabouraud’s agar, which facilitates
the appearance of the slow-growing fungi by inhibiting the growth of
bacteria in the specimen.
• Inhibition of bacterial growth is due to the low pH of the medium and
to the penicillin, streptomycin, and cycloheximide that are frequently
added.
• The appearance of the mycelium and the nature of the asexual spores
are frequently sufficient to identify the organism
42.
43. DNA probe tests
• Tests involving DNA probes can identify colonies growing in culture at
an earlier stage of growth than can tests based on visual detection of
the colonies.
• As a result, the diagnosis can be made more rapidly.
• At present, DNA probe tests are available for Coccidioides,
Histoplasma, Blastomyces, and Cryptococcus.
44.
45. serologic tests
• Tests for the presence of antibodies in the patient’s serum or spinal
fluid are useful in diagnosing systemic mycoses but less so in
diagnosing other fungal infections.
• As is the case for bacterial and viral serologic testing, a significant rise
in the antibody titer must be observed to confirm a diagnosis.
• The complement fixation test is most frequently used in suspected
cases of coccidioidomycosis, histoplasmosis, and blastomycosis. In
cryptococcal meningitis, the presence of the polysaccharide capsular
antigens of C. neoformans in the spinal fluid can be detected by the
latex agglutination test.
46. delayed hypersensitivity skin test
• Infection with the systemic fungi, such as Histoplasma and
Coccidioides, can be detected by using skin tests.
• An antigen extracted from the organism injected intradermally elicits
a delayed hypersensitivity reaction, manifested as induration
(thickening) of the skin.
• Note that a positive skin test only indicates that infection has
occurred, but it is not known whether that infection occurred in the
past or at the present time. Therefore, a positive skin test does not
indicate that the disease the patient has now is caused by that
organism.