Lateral flow assays are the most robust, mature immuno sensor available today. Performance in some applications has historically been limited by difficulties in multiplexing and quantification. Novel approaches have been developed and commercialized in recent years that allow for the development and manufacturing of highly multiplexed arrays in lateral flow assays. The patented Symbolics (tm) approach is one such methodology. Symboics (tm) allows for the creation of arrays in lateral flow fields that develop evenly, allowing in turn for the creation of highly complex features such as letters and symbols and also allows for creation of multiplex assays with advanced features such as internal controls. This presentation introduces the principles of multiplexed arraying and the Symbolics technology
This presentation covers an introduction to UPLC, its general chemistry, and laws behind it. It also discusses the instrumentation of UPLC, advantages, disadvantages, and application of UPLC.
This presentation covers an introduction to UPLC, its general chemistry, and laws behind it. It also discusses the instrumentation of UPLC, advantages, disadvantages, and application of UPLC.
Presented By :- Raghav Sharma
Class :- M. Pharm, 1st sem.
Department :- Pharmaceutics
Institute :- Parul Institute of Pharmacy
Content :-
Instrumentation and working of flame photometry
Flame atomizer
Nebulizer
Atomizer burner
Monochromator
Detector
Amplifier
Advantages
Disadvantages
Reference
In this slide contains types of HPLC Columns, Plate theory and Van Deemter Equation.
Presented by : Malarvannan.M (Department of pharmaceutical analysis).
RIPER,anantpur.
Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture (the relative amounts of such components can also be determined). In some situations, GC may help in identifying a compound. In preparative chromatography, GC can be used to prepare pure compounds from a mixture
High Performance Thin Layer Chromatography (HPTLC) instrumentationMadhuraNewrekar
HPTLC is an advancement of TLC. It is a high performance liquid chromatography with automation compared to Thin Layer Chromatography(TLC).Speed, Efficiency and Accuracy are important advantages. Evaluation time is less due to updated automation in instrumentation.
Steps involved in HPTLC and the materials and instruments required in those steps are described in brief.
a type of an analyzer used in mass spectrometer. separates the ions based on mass to charge ratios. useful for the detection of ions present in the sample
Presented By :- Raghav Sharma
Class :- M. Pharm, 1st sem.
Department :- Pharmaceutics
Institute :- Parul Institute of Pharmacy
Content :-
Instrumentation and working of flame photometry
Flame atomizer
Nebulizer
Atomizer burner
Monochromator
Detector
Amplifier
Advantages
Disadvantages
Reference
In this slide contains types of HPLC Columns, Plate theory and Van Deemter Equation.
Presented by : Malarvannan.M (Department of pharmaceutical analysis).
RIPER,anantpur.
Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture (the relative amounts of such components can also be determined). In some situations, GC may help in identifying a compound. In preparative chromatography, GC can be used to prepare pure compounds from a mixture
High Performance Thin Layer Chromatography (HPTLC) instrumentationMadhuraNewrekar
HPTLC is an advancement of TLC. It is a high performance liquid chromatography with automation compared to Thin Layer Chromatography(TLC).Speed, Efficiency and Accuracy are important advantages. Evaluation time is less due to updated automation in instrumentation.
Steps involved in HPTLC and the materials and instruments required in those steps are described in brief.
a type of an analyzer used in mass spectrometer. separates the ions based on mass to charge ratios. useful for the detection of ions present in the sample
One of the main challenges for a versatile application of monitoring technologies in the
veterinary and food industry is to develop fast, quantitative and low cost devices that
can be used with minimal expertise. Most of the diagnostic technologies in use today
require laboratory facilities, expensive equipments and trained personnel. During the
last decade, a few technologies have been proposed and developed that fulfill most
requirements of versatility mentioned above. One of the most promising approaches is
the lateral flow immunoassay technique.
Design and development of lateral flow assays for field useBrendan O'Farrell
Lateral flow assays are the most mature, stable, field deployable sensor available today. They can be used in myriad applications, from medical diagnostics to veterinary testing, agriculture, bio-defense, food testing and environmental testing, to name a few. Performance of these assays has evolved to the point where they can equal that of much more highly complex laboratory based diagnostic test formats. They can be quantitative, multiplexed and highly sensitive if developed and manufactured correctly. Design and development of these high performance lateral flow systems requires a from-first-principles approach, with an eye to optimizing the system for reproduciblity and sensitivity. Employing user - centered design and development practices greatly improves the odds of successful commercialization. DCN Diagnostics (formerly Diagnostic Consulting Network) develops high performance lateral flow assay systems for use in any environment. Our concurrent design and development process, employing cross functional teams of industrial design and mechanical engineers alongside our immunoassay development teams, ensures that the right product is developed the right way to allow for most efficient regulatory approval and commercialization. Our development process is fully design controlled and operates under our ISO 9001:2008 and EN 13485 compliant quality system. With literally hundreds of assays developed, and transferred to manufacturing, DCN is the go-to supplier of contract development services in the point of care diagnostic test market. Additionally, our consulting teams can provide a deep strategic vision to our clients, assisting in all aspects of product development and commercialization, including regulatory affairs and clinical trial management. This presentation describes DCN's development process and illustrates the benefit of a user centered design process in creating the right rapid assay for your market, focusing on field deployed tests for clinical diagnostics, veterinary testing and bio-defense applications. A variety of case studies are shown, illustrating the principles discussed.
Keynote "Practical case-studies of Industry 4.0 implementation in the global wire and cable manufacturer community" of Clobbi CEO Dmitry Shapovalov that was held 13 Jun 2019 @CRU 2019 Brussels
Clobbi CEO Dmitry Shapovalov Keynote @CRU 2019 Brussels "Practical case-studi...Clobbi
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Clobbi CEO Dmitry Shapovalov Keynote @CRU 2019 Brussels "Practical case-studi...Clobbi
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The Foundation of a reliable product is a reliable PCB
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Have a comprehensive strategy for selecting and qualifying PCB suppliers
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Performing effective on site audits is a critical component of that strategy.
Use of Machine Intelligence in manufacturing industry poses a special challenge due to a wide range of use cases, inherent complexity in data collection, availability of information and disconnect between information islands in different manufacturing steps.
Within our talk we present several machine intelligence projects we did in the manufacturing industry, which helped our customers in product quality improvement, reduction of cost and better asset management. We will talk about the used methodologies, the results achieved and the lessons learned from these projects. We will specifically focus on the importance of process and business knowledge for successful implementation of any industrial project.
Mobile Market and Technology Overview Dubai 2017Dan Geiger
I used this to provide an overview of the mobile market with some mobile specific traffic management use cases. This was delivered to a technically inclined group of network and cloud providers in the middle eastern telecom and enterprise spaces at Procera's 2 day customer event.
Chemists need numerous measurements quickly to assess the potential of a given compound within a therapeutic area. Instrumentation is expensive and of limited availability in even the most well funded organizations. The ability to share instrumentation helps mitigate equipment costs but poses special challenges to allow access and use by everyone across an organization. A solution to create open access purification equipment is presented using a Z180 microprocessor, touch screen interface, and an embedded system program written in C.
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Learn more on the opportunities and hurdles of intensified, connected or continuous processing. 30 US and European biomanufacturers were interviewed to understand the likely future adoption of ‘Next Generation Bioprocessing’.
Discover the animated version! http://www.merckmillipore.com/INTL/en/20180329_155610?bd=1&tab=2
Visit the BioContinuum™ Platform webpage now! http://www.merckmillipore.com/biocontinuum
A Guide to Instrumentation for Ethanol Fuel ProductionMead O'Brien, Inc.
Ethanol, the common name for ethyl alcohol, is fuel grade alcohol that is produced through the fermentation of simple carbohydrates by yeasts. Fueled by growing environmental, economic, and national security concerns, U.S. ethanol production capacity has nearly doubled in the past six years, and the Renewable Fuel Association (RFA) projects another doubling of the industry by 2012. Ethanol can be made from renewable feedstock’s such
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Better Bioprocessing Efficiency Through Centralized OrchestrationMerck Life Sciences
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Detailed description:
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Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
1. Patented technology facilitating multiplexing, quantification
and alpha-numeric result generation in rapid assays
Patented
www.Symbolicsdx.com
2. Intuitive Results, Better Results
SymbolicsTM
pixilation technology allows for new approaches to
result generation in lateral flow, including:
1. Geometric symbols
2. Alpha -numeric symbols.
3. Multiplexing (spot arrays or other formats)
4. Other advanced design features
3. Background
• Symbolics LLC was founded in 2010 as a joint venture between
DCN Inc and BioDot Inc
• Wholly owned by DCN and BioDot
• Key management and joint development team in place
• Initial patent allowed, January 2013
• Subsequent filings in progress
4. The Problem
• Lateral flow assays are limited to linear results,
created by the placement of lines of capture
reagents across the width of a flow path
• User Error: Linear results are non-intuitive
and can be difficult to interpret
• Multiplexing and quantification are hard:
– Lines are stacked behind one another
– Line bleed occurs
– Cross reactivity issues are worsened
– Flow rate decreases non-linearly with
distance from origin: quantification of
multiplexed lines is difficult and slow
The Problem with Lines
5. The Problem
Traditional manufacturing methods create
large structures in flow paths
Large, discreet linear features cause flow
pattern disturbances, preventing formation
of features behind them over relatively large
distances in the direction of flow.
High AffinityLow AffinityNo Affinity
Creating other types of features using standard production methods
results in imperfect feature geometry
Strong leading edge binding due to high affinity binding reagents is
extreme in drop sizes used in standard approaches. Reagents that are
useful for lateral flow (with decent affinity) will not form complete
features if spotted on a membrane in standard spot sizes
6. In Other Words….
• The industry has stuck with lines because they are the only way
to produce even, reproducible features in a lateral flow path.
• Alternatives currently in market, such as the “+” symbol rely on
pre-printed ink features to create part of the symbol
• Digital devices returning easily interpreted alpha-numeric results
such as “Pregnant” require expensive, patented reader
technologies in the cassettes, resulting in complexity and margin
hits for manufacturers
Pre-printed ink line
7. The Unseen Impact of Linear Results
• Limited success of lateral flow systems in multiplexed or quantitative
applications
• Limitations on user-friendliness of consumer assays
• Limitation in overall market growth
Average 7% CAGR does not account for the growing potential in
consumer markets, for multiplexed applications such as Traumatic
Brain Injury, Kidney, Cardiac or Dehydration panels, or for other novel
quantitative applications: There is a lot of untapped potential.
8. The Solution: Pixelation
• The problems of incomplete feature formation can be overcome through the
use of appropriately sized features (“pixels”) spaced at appropriate distances in
the flow path.
• This makes the formation of larger, complex features possible.
• Pixels are created using precise methods for dispensing and spacing discrete
spots of protein in the lateral flow field. Volumes range from the picoliter range
to the low nanoliter range at carefully controlled pitch (spacing).
• Dispensing is done using a variety of technologies including piezo-electric or
solenoid-based dispensers.
9. Intuitive Results, Better Results
SymbolicsTM
pixilation technology allows for new approaches to
result generation in lateral flow, including:
1. Geometric symbols
2. Alpha -numeric symbols.
3. Multiplexing (spot arrays or other formats)
4. Other advanced design features
10. The Opportunity
• Revolutionize entire rapid testing market segments by
facilitating the development and manufacturing of novel lateral
flow assays with a range of advantages:
– A new generation of user-centered consumer assays that
are intuitive, simple to interpret but do not require digital
interpretation
– Better quantification
– Higher dynamic range
– Internal controls
– Multiplexing of analytes, at virtually any density required for
diagnostics or testing in most market segments (high or low)
– Lower reagent usage
11. Where we are
• 1 issued US patent: 13/343,681: “Lateral flow assays using two
dimensional features”
• 1 published PCT: PCT/US2012/021586
• Patent portfolio expansion underway with multiple US and
international filings
• Licensing strategy in place
• Licenses and development support available
12. Offering a complete solution or a simple license
*Licenses are available without
using support services offered by
DCN or equipment offered by
BioDot
13. Getting Started
• Symbolics technology is available for license and integration into
your assay development programs
• DCN and BioDot are available if so desired to support your assay
development or re-formatting program
14. Licensing
A two level license is offered
1.Development License
1. Non exclusive
2. Low cost
3. Limited term
4. Patent grant-back
2.Manufacturing and Commercialization License
1. To be negotiated
16. Assay Development Strategy
• Assay development follows essentially the same process as
standard lateral flow
• Development requires higher fidelity dispensing equipment
• All of the same labels, reagents and many of the same materials
can be applied
• For higher fidelity quantitative applications, some alternative
material choices may yield better results
• An understanding of the affinity of the reagents being used is
useful given the interaction between reagent affinity and
feature spacing and size
• Assistance can be provided by DCN Inc if required
17. Manufacturing
• Manufacturing process design is similar to standard lateral flow
• Dispensing processes require higher fidelity in terms of
placement and volume
• Dispensing processes must be robust to ensure fidelity of
feature formation
• Consideration must be given to feature position during cutting,
so tracking features (fiducials) must be included in the card
design
• Virtually all other processes will stay the same as in standard
lateral flow
• Equipment can be provided by BioDot Inc if required
18. Table top equipment for R&D and batch manufacturing
Reel-to-reel equipment for high volume production
24. 2. Alpha – Numeric Symbols
Sandwich Applications
Drop Vol: 7nL
Pitch: 0.5mm
6mm Strips
BioJet Plus
CON: 0.125mg/ml protein A
H1: 4mg/ml GP041
H2: 2mg/ml GP036
Conjugate: 10 uL of 8ug/ml
protein A
Sample: 5uL human plasma positive
for HIV-1, 100uL HIV Running Buffer
Sample: 5uL human plasma positive
for HIV-2, 100uL HIV Running Buffer