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Multiparametric in qPCR diagnostics, what's that? | Exopol

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Sergio Exopol
Sergio Exopol

Exopol has developed the new EXOone qPCR kits in Real Time. When we talk about its features, we always say that they are "multiparametric", but, do you know what it means? Multiparametric is the name we use to refer that you can combine different pathogens in different wells at the same cycler run as all our assays have the same thermal profile. In the presentation you can see an example of a trial with our EXOone kit.

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Multiparametric: Different parameters in a plate at a single thermocycler run. Combine different pathogens in different wells at the same cycler run as all our assays have the same thermal profile. Just need two reading channels: - FAM (for the pathogen) - HEX/VIC (for the endogenous control)
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Multiparametric in qPCR diagnostics, what's that? | Exopol

  • 1.
  • 2. Multiparametric: Different parameters in a plate at a single thermocycler run. Combine different pathogens in different wells at the same cycler run as all our assays have the same thermal profile. Just need two reading channels: - FAM (for the pathogen) - HEX/VIC (for the endogenous control)
  • 4. Get tons of information in less than 2 hours…! Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 5. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae Patógeno (FAM) Endogenous Control (HEX/VIC) Both in the same well and read in different channels
  • 6. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 7. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 8. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 9. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 10. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 11. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 12. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 13. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 14. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 15. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 16. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 17. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 18. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 19. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 20. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 21. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 22. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 23. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 24. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 25. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 26. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 27. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae
  • 28. Porcine circovirus Toxoplasma gondii Pathogenic Leptospira Coxiella burnetii Chlamydia abortus Neospora caninum Mycoplasma ovipneumoniae Porcine parvovirus Besnoitia besnoiti Mycoplasma mycoides E. Coli eae Chlamydiaceae Mixoma virus Brachyspira hyodisenteriae Brachyspira pilosicoli F4 (E. coli virulence factor) F18 (E. coli virulence factor) STa Stx2 STb STx2e (E. coli virulence factor) GAD (E. coli control) LT (E. coli virulence factor) Mycoplasma Agalactiae