The document discusses moving boundary electrophoresis, a technique for separating chemical compounds through the migration of charged particles in a solution under an electric field. It outlines the principles, types, instrumentation, advantages, disadvantages, and applications of electrophoresis, emphasizing its use in separating proteins and analyzing macromolecules in various fields like enzymology and immunology. The technique is noted for its ability to detect minute concentrations of samples but is also criticized for its high cost and low resolution.

1. Presented by
Roshan Yadav
M.pharm(Pharmaceutics).
Submitted to
Dr. Yele Vidyasrilekha.
MOVING BOUNDARY ELECTROPHORESIS

2. Contents
• Introduction
• Principle of Electrophoresis
• Types of Electrophoresis
• Moving Boundary electrophoresis
• Instrumentation
• Advantages and Disadvantages
• Application

3. Introduction
• Electrophoresis is defined as the migration of charged particle
through a solution under the influence of an external electric feild.
• Ions that are suspended between two electrodes tends to travel
towards the electrodes that posses opposite charge.

4. Principle
• The fundamental principle of electrophoresis is that any charged ions
or molecules migrate towards oppositely charged electrode under the
influence of external electric field.
• The rate of migration of these ions depends on its charge size and the
applied electric field.

5. Types of electrophoresis
1)zone electrophoresis
a) Paper electrophoresis
b) Gel electrophoresis
c) Thin layer electrophoresis
2) Moving boundary electrophoresis
a) Capillary
b) Isotachoporesis
c) Isoelectric electrophoresis
d) Immuno electrophoresis

6. Moving boundary electrophoresis
• Moving boundary electrophoresis is technique for separation of
chemical compounds by electrophoresis in a free solution.
• Moving boundary electrophoresis is developed by Tiselius.
• The principle of moving boundary electrophoresis is that there is
motion of charge particle through stationary liquid under the
influence of an electric field.

7. Instrumentation
• Apparatus consist of “U” tube with electrode located at the two ends
used to apply on electric field.
• The lower part of the cells is filled with lyophilic solution under
examination sometimes the sample solution is introduced into the
bottom of the “U” tube through capillary arm while upper part
contains only the buffer solution.
• Care must be taken to minimize the disturbing effect of convection
caused by an increase in temperature during the passage of current
through the solution.
• The apparatus is placed in constant temperature .

9. Advantages
• Biologically active fraction can be removed without the use of
denaturing agent.
• Minute concentration of sample can be detected.
• A reference method for measuring electrophoretic mobility.

10. Disadvantages
• Expensive.
• Resolution of the techniques is low due to overlapping of the sample
component.

11. Applications
• To separate proteins and peptides.
• For research in enzymology, immunology.
• Used to quantitative analysis of complex mixture of macromolecules.

12. THANK YOU