Electrophoresis is a technique used to separate charged molecules such as proteins and nucleic acids based on their charge and size ratios. It involves applying an electric field to migrate molecules through a medium like gel or paper. Initially developed in the 1930s to separate serum proteins, it has since been used for applications like human genome sequencing and disease diagnosis. Improvements to the technique include capillary electrophoresis using narrow tubes, isoelectric focusing based on a molecule's isoelectric point, and immuno electrophoresis to study antibody-antigen reactions.
Paper electrophoresis
gel electrophoresis
capillary electrophoresis
zone electrophoresis
moving boundary electrophoresis
iso electric focusing electrophoresis
Paper electrophoresis
gel electrophoresis
capillary electrophoresis
zone electrophoresis
moving boundary electrophoresis
iso electric focusing electrophoresis
it is a short ppt. on Electrophoresis. Which gives it's defination, Types of electrophoresis ; Like:- Zone electrophoresis, Isoelectric focussing, Immunoelectrophoresis. and their images.
INTRODUCTION, DEFINATION OF ELECTROPHORESIS, ELECTROPHORESIS PRINCIPLE, TYPES OF ELECTROPHORESIS, FREE ELECTROPHORESIS, ZONE ELECTROPHORESIS,PAPER ELECTROPHORESIS, WORKING OF PAPER ELECTROPHORESIS, PROCEDURE FOR PAPER ELECTROPHORESIS, VISUALISATION, FACTORS AFFECTING SEPARATION OF MOLECULES, APPLICATIONS, working of paper electrophoresis ,procedure for paper electrophoresis ,visualisation ,factors affecting separation of molecules ,applications ,forensics ,dna fingerprinting ,molecular biology ,microbiology information about the organisms ,biochemistry mapping of cellular components ,paper electrophoresis is also used in study of sic ,hemoglobin abnormalities ,separation of blood clotting factors ,serum plasma proteins from blood sample ,used in separation and identification of alkaloids ,used for testing water samples ,toxicity of water ,drug industry to determine presence of illelgal drUGS
What is Electrophoresis?
Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.
This electrokinetic phenomenon was first observed in 1807 by Russian professors Peter Ivanovich Strakhov and Ferdinand Frederic Reuss (Moscow State University), who noticed that the application of a constant electric field caused clay particles dispersed in water to migrate.
Electrophoresis of positively charged particles (cations) is called cataphoresis while electrophoresis of negatively charged particles (anions) is called anaphoresis.
Electrophoresis is the movement of charged particles through an electrode when subjected to an electric Field
Cations move towards cathode
Anions move towards anode
By this technique solutes are separated by their different rates of travel through an electric field.
Commonly used in biological analysis, particularly in the separations of proteins, peptides and nucleic acids
it is a short ppt. on Electrophoresis. Which gives it's defination, Types of electrophoresis ; Like:- Zone electrophoresis, Isoelectric focussing, Immunoelectrophoresis. and their images.
INTRODUCTION, DEFINATION OF ELECTROPHORESIS, ELECTROPHORESIS PRINCIPLE, TYPES OF ELECTROPHORESIS, FREE ELECTROPHORESIS, ZONE ELECTROPHORESIS,PAPER ELECTROPHORESIS, WORKING OF PAPER ELECTROPHORESIS, PROCEDURE FOR PAPER ELECTROPHORESIS, VISUALISATION, FACTORS AFFECTING SEPARATION OF MOLECULES, APPLICATIONS, working of paper electrophoresis ,procedure for paper electrophoresis ,visualisation ,factors affecting separation of molecules ,applications ,forensics ,dna fingerprinting ,molecular biology ,microbiology information about the organisms ,biochemistry mapping of cellular components ,paper electrophoresis is also used in study of sic ,hemoglobin abnormalities ,separation of blood clotting factors ,serum plasma proteins from blood sample ,used in separation and identification of alkaloids ,used for testing water samples ,toxicity of water ,drug industry to determine presence of illelgal drUGS
What is Electrophoresis?
Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.
This electrokinetic phenomenon was first observed in 1807 by Russian professors Peter Ivanovich Strakhov and Ferdinand Frederic Reuss (Moscow State University), who noticed that the application of a constant electric field caused clay particles dispersed in water to migrate.
Electrophoresis of positively charged particles (cations) is called cataphoresis while electrophoresis of negatively charged particles (anions) is called anaphoresis.
Electrophoresis is the movement of charged particles through an electrode when subjected to an electric Field
Cations move towards cathode
Anions move towards anode
By this technique solutes are separated by their different rates of travel through an electric field.
Commonly used in biological analysis, particularly in the separations of proteins, peptides and nucleic acids
electrophoresis: movement of charge particles in a gel under the influence of an electric field, principle, factors, apparatus, types , application, advantage and disadvantage.
This presentation contain the information about gel electrophoresis method , instruments & types.
Electrophoresis is a method through biological molecules are separated by applying an electric field.
Main purpose of this method is to determine the number , amount & mobility of biological component.
There are some internal & external factors that affects the process of electrophoresis.
The bio-molecules have charge on it & when we apply an electric field , the charge particles move to the opposite cathode. In this way, charge particles are separated
There are 3 types of gels that use in this process .
In this buffers are also used which provide ions that carry a current.
Electrophoresis is a separation technique that is based on the movement of charged particles in an electric field.
Electrophoresis is an analytical method of separating charged particles based on their relative mobilities in an electric field
It's my prepared presentation on paper and gel electrophoresis for m.pharm students of 1st year pharmaceutics department.
I hope it will help you well for study.
If you like it then please appreciate it.
Thank you 🤗
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Electrophoresis
1.
2. Electrophoresis
• INTRODUCTION:
• Electrophoresis: It is a separation method based on
differential rate of migration of charged species in an applied
dc electric field.
• Rate of migration
• Depends on charge and size
• Separation based on differences in
charge-to-size ratios
3. Historical background
• Initially developed by Arne Tiselius in the 1930’s –
Separated serum proteins
• Slab gel electrophoresis: developed in the 1950’s
• Capillary electrophoresis: – developed in the 1980’s –
Narrow bore tubes used
• Applications of electrophoresis: – Separation of
proteins and nucleic acids (single nucleotide
differentiation capability!) – The human genome
Project
• • Human DNA: ~three billion nucleotides
4. Electrophoresisprinciple:
• Principle involves electrophoretic mobility of
ions.
• It has been found that the migration of
velocity(v) (cms-1) of molecule in an electric
field is given by
v = (µe E)
µ = electrophoretic mobility
E = strength of electric field
5. • The value of (E) depend upon:
• Charge of the analyte ion.
• The friction regarding force.
• Which includes
• Size and shape of ion.
• Viscosity of the medium in
which migration occur.
6. Instrumentation:
In most electrophoresis units, the gel is mounted between two buffer
chambers containing separate electrodes so, that the only electrical
connection between the two chambers is through the gel.
12. Type of electrophoresis
• Zone electrophoresis:
• Paper electrophoresis
• Gel Electrophoresis
• Thin layer Electrophoresis
• Cellulose acetate Electrophoresis
• Moving boundary electrophoresis:
• Capillary electrophoresis
• Isotachophoresis
• Isoelectric focusing
• Immuno Electrophoresis
13. Zone Electrophoresis
• It involves migration of charge particle on the supporting
media.
• Such as paper, cellulose acetate membrane, Starch gel,
poly acrylamide.
• supporting media always saturated with buffer.
• Advantages:
• Useful in biochemical investigation.
• Cost is low and easy maintenance.
• Disadvantages:
• Unsuitable for accurate mobility.
14. Moving boundary electrophoresis
• It contain U shaped arrangement.
• It does not need any supporting media to move.
• High mobility, fast migration.
• Low mobility , slow migration.
• Used for separation of protein as well as inorganic
substances.
15. Capillary electrophoresis:
• Capillary electrophoresis is a technique of performing
electrophoresis in buffer filled, narrow-bore capillaries
normally from 25-100 mm internal diameter.
• High voltage typically 10-30 kV.
•
16. Isotachophoresis
• This technique depend upon the development of potential
gradient.
• Based on principle of moving boundary electrophoresis.
•
17. Isoelectric focusing
• All proteins have an isoelectric point.
• This will be in zwitter ion form no electric charge so,
further moment will be cease.
• Protein migrate to the point where its net charge is 0, so,
isoelectric pH.
• Use for enzymology, immunology,
cytology and taxonomy.
18. Immuno Electrophoresis
• Antibodies are produce by immune system in response to
foreign macromolecules (antigen).
• When electric potential is applied to study antibody-
antigen reactions, it is called Immuno electrophoresis.
• Mainly used for separating proteins and poly peptides.
• Used in forensic , clinic and human genome laboratories
for separation.
• Used in identification of serum protein in research in
enzymology, membrane biochemistry, microbiology and
immunology.
19. Detectors used in electrophoresis
• UV absorption detector: used for uv absorbing
materials.
• Conductivity detector: used for measuring electric
resistance and conductivity.
• High frequency detector: Used for field strength.
• Thermometric detector: Used for temperature and heat
production. By measuring temperature of particular ionic
species location can be found out.
20. Application of elecrophoresis
• Gene and genome Analysis: Electrophoresis used for
analyzing gene. The analyzed DNA used in forensic
investigations.
• Protein and proteome analysis: Amount of protein in blood
and urine can be analyzed.
• Antibiotic Analysis: Electrophoresis used for new type of
antibiotics such as penicillin (drug used for bacterial
infection).
• Vaccine Analysis: electrophoresis used for purification,
processing and analyzing various vaccine.
21. By :
Scholar Hina Islam Chishty
Organic chemistry
Institute of Chemistry
E-mail: chishtyislam555@gmail.com
Shah Abdul latif university
Khairpur Mirs` Sindh
Pakistan