Prepared By : Sanjay Sharma (Sr. Analyst) For 4 important concepts related to method validation- instrument detection limit, method detection limit, below detection limit and recovery. When signals from absolute analytes are considerably greater than signals produced by analytical instruments, it is referred to as the instrument detection limit. Method detection limit is the least amount of a substance in a sample which can be measured and if substance is even below that level, it is referred as below detection limit.