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Substances needed by the cellsSubstances needed by the cells
 WaterWater
 OxygenOxygen
 NutrientNutrient
– GlucoseGlucose
– Amino acidAmino acid
– MineralMineral
– Fatty acidFatty acid
– VitaminVitamin
 The substances have to move across theThe substances have to move across the
membranemembrane
Substances to be eliminate fromSubstances to be eliminate from
the cellsthe cells
 Carbon dioxideCarbon dioxide
 UreaUrea
 ToxinToxin
Fluid Mosaic ModelFluid Mosaic Model
 1972 - Singer and Nicolson called the1972 - Singer and Nicolson called the
membrane amembrane a “Fluid Mosaic Model”.“Fluid Mosaic Model”.
 Mosaic:Mosaic: different proteinsdifferent proteins
embedded inembedded in thethe
phospholipids.phospholipids.
 Fluid:Fluid: proteins and phospholipids canproteins and phospholipids can
move freely in themove freely in the
membrane.membrane.
 Components of a phospholipidComponents of a phospholipid
bilayer.bilayer.
1.1. phospholipidsphospholipids
2.2. proteins -proteins - enzymes, receptors,enzymes, receptors,
transport.transport.
3.3. glycolipidsglycolipids
4.4. glycoproteinsglycoproteins
5.5. carbohydratescarbohydrates
6.6. cholesterolcholesterol
Plasma MembranePlasma Membrane
 Boundary that separates theBoundary that separates the living cellliving cell
from it’sfrom it’s non-livingnon-living surroundings.surroundings.
 Phospholipid bilayerPhospholipid bilayer
 AmphipathicAmphipathic - having both:- having both:
hydrophilic headshydrophilic heads
hydrophobic tailshydrophobic tails
 ~8 nm thick~8 nm thick PhospholipidPhospholipid
 Controls trafficControls traffic into and out of the cellinto and out of the cell
withwith phospholipidsphospholipids andand transporttransport
proteinsproteins..
 Selectively permeableSelectively permeable
 >>> semi-permeable>>> semi-permeable
Transport proteinTransport protein
What is Selective Permeability?What is Selective Permeability?
 TheThe propertyproperty ofof biological membranesbiological membranes
which allows some substances to crosswhich allows some substances to cross
more easily than others.more easily than others.
Transport ProteinsTransport Proteins
 TransportsTransports moleculesmolecules oror ionsions acrossacross
biological membranesbiological membranes
 Two types:Two types:
– Carrier proteinsCarrier proteins
– Pore proteinsPore proteins
Through phospholipids layer:
Non polar molecules = Oxygen. Carbon
dioxide, water , lipid soluble molecules
By pore – proteins : water
soluble molecules and ions
By carrier- proteins and energy : ions.
By carrier- proteins : glucose, amino acids.
 Passive transport:Passive transport: NONO energyenergy is expended.is expended.
– Simple diffusion – gases,Simple diffusion – gases,
– Osmosis – waterOsmosis – water
 facilitated diffusion:facilitated diffusion:
type oftype of passive transportpassive transport which useswhich uses transporttransport
proteins.proteins.
–– glucose and amino acidglucose and amino acid
 Active transport :Active transport :
– Using carrier protein and energy – ionsUsing carrier protein and energy – ions
DiffusionDiffusion
The net movement of a substance
(molecules) down a concentrationconcentration
gradientgradient from an area of highhigh
conconcentrationcentration to an area of lowlow
concentrationconcentration.
Facilitated diffusionFacilitated diffusion
 Allows diffusion of large, membrane insoluble compoundsAllows diffusion of large, membrane insoluble compounds
such as sugars and amino acidssuch as sugars and amino acids
 Does not require energy (passive)Does not require energy (passive)
 Highly SelectiveHighly Selective
 Substance binds to membrane- transport proteinSubstance binds to membrane- transport protein
 Fully reversible - molecules may enter the cell and leaveFully reversible - molecules may enter the cell and leave
the cell through the transport protein.the cell through the transport protein.
 Particles move from areas of high concentration to areas ofParticles move from areas of high concentration to areas of
low concentration.low concentration.
 Movement rate of particles will saturateMovement rate of particles will saturate
– Maximum rate limited by number of transportersMaximum rate limited by number of transporters
– Once all transporters are operating at 100%, an increase inOnce all transporters are operating at 100%, an increase in
concentration will not increase rateconcentration will not increase rate
Active TransportActive Transport
 Movement across membrane with anMovement across membrane with an
energy cost (usually against concentrationenergy cost (usually against concentration
 Used to pump specific compounds in orUsed to pump specific compounds in or
out of the cellout of the cell
 Requires energy to overcome theRequires energy to overcome the
concentration gradient or to allow a largeconcentration gradient or to allow a large
or charged particle to cross membraneor charged particle to cross membrane
 Requires specific integral membraneRequires specific integral membrane
proteinsproteins
– Can be saturated like facilitated diffusionCan be saturated like facilitated diffusion
proteinsproteins
– The energy requirement distinguishes activeThe energy requirement distinguishes active
transport from facilitated diffusiontransport from facilitated diffusion
OsmosisOsmosis
 The movement ofThe movement of waterwater acrossacross
selectively permeable membranesselectively permeable membranes..
 TheThe waterwater moves from amoves from a lowlow
concentration areaconcentration area toto highhigh
concentration areaconcentration area
Cell membranes are
completely permeable to
water, therefore, the
environment the cell is
exposed to can have a
dramatic effect on the cell.
Hypotonic solutionHypotonic solution
 Less soluteLess solute
 More waterMore water
 Compare to cytoplasm of the cellCompare to cytoplasm of the cell
Hypertonic solutionHypertonic solution
 More soluteMore solute
 Less waterLess water
 Compare to cytoplasm of the cellCompare to cytoplasm of the cell
Isotonic solutionIsotonic solution
 Equal concentration compare toEqual concentration compare to
cytoplasm of the cellcytoplasm of the cell
Hypertonic Solution:
• Solute concentration of solution higher than cell
• More dissolved particles outside of cell than
inside
of cell
• Hyper = more (think hyperactive); Tonic =
dissolved particles
• Water moves out of cell into solution
• Cell shrinks
Hypotonic Solution:
• Solute concentration of solution lower than cell
• Less dissolved particles outside of cell than
inside of cell
• Hypo = less, under (think hypodermic,
hypothermia);
Tonic = dissolved particles
• Water moves into cell from solution
• Cell expands (and may burst)
Isotonic Solution:
Solute concentration of solution equal to that of cell
No net water movement
Why the use of
excess fertilizer caused
wilting in plant?
Explain these preservation process

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Movement of substances

  • 1.
  • 2.
  • 3. Substances needed by the cellsSubstances needed by the cells  WaterWater  OxygenOxygen  NutrientNutrient – GlucoseGlucose – Amino acidAmino acid – MineralMineral – Fatty acidFatty acid – VitaminVitamin  The substances have to move across theThe substances have to move across the membranemembrane
  • 4. Substances to be eliminate fromSubstances to be eliminate from the cellsthe cells  Carbon dioxideCarbon dioxide  UreaUrea  ToxinToxin
  • 5.
  • 6. Fluid Mosaic ModelFluid Mosaic Model  1972 - Singer and Nicolson called the1972 - Singer and Nicolson called the membrane amembrane a “Fluid Mosaic Model”.“Fluid Mosaic Model”.  Mosaic:Mosaic: different proteinsdifferent proteins embedded inembedded in thethe phospholipids.phospholipids.  Fluid:Fluid: proteins and phospholipids canproteins and phospholipids can move freely in themove freely in the membrane.membrane.
  • 7.
  • 8.
  • 9.
  • 10.  Components of a phospholipidComponents of a phospholipid bilayer.bilayer. 1.1. phospholipidsphospholipids 2.2. proteins -proteins - enzymes, receptors,enzymes, receptors, transport.transport. 3.3. glycolipidsglycolipids 4.4. glycoproteinsglycoproteins 5.5. carbohydratescarbohydrates 6.6. cholesterolcholesterol
  • 11. Plasma MembranePlasma Membrane  Boundary that separates theBoundary that separates the living cellliving cell from it’sfrom it’s non-livingnon-living surroundings.surroundings.  Phospholipid bilayerPhospholipid bilayer  AmphipathicAmphipathic - having both:- having both: hydrophilic headshydrophilic heads hydrophobic tailshydrophobic tails  ~8 nm thick~8 nm thick PhospholipidPhospholipid
  • 12.
  • 13.
  • 14.
  • 15.  Controls trafficControls traffic into and out of the cellinto and out of the cell withwith phospholipidsphospholipids andand transporttransport proteinsproteins..  Selectively permeableSelectively permeable  >>> semi-permeable>>> semi-permeable Transport proteinTransport protein
  • 16. What is Selective Permeability?What is Selective Permeability?  TheThe propertyproperty ofof biological membranesbiological membranes which allows some substances to crosswhich allows some substances to cross more easily than others.more easily than others.
  • 17. Transport ProteinsTransport Proteins  TransportsTransports moleculesmolecules oror ionsions acrossacross biological membranesbiological membranes  Two types:Two types: – Carrier proteinsCarrier proteins – Pore proteinsPore proteins
  • 18. Through phospholipids layer: Non polar molecules = Oxygen. Carbon dioxide, water , lipid soluble molecules By pore – proteins : water soluble molecules and ions By carrier- proteins and energy : ions. By carrier- proteins : glucose, amino acids.
  • 19.  Passive transport:Passive transport: NONO energyenergy is expended.is expended. – Simple diffusion – gases,Simple diffusion – gases, – Osmosis – waterOsmosis – water  facilitated diffusion:facilitated diffusion: type oftype of passive transportpassive transport which useswhich uses transporttransport proteins.proteins. –– glucose and amino acidglucose and amino acid  Active transport :Active transport : – Using carrier protein and energy – ionsUsing carrier protein and energy – ions
  • 20. DiffusionDiffusion The net movement of a substance (molecules) down a concentrationconcentration gradientgradient from an area of highhigh conconcentrationcentration to an area of lowlow concentrationconcentration.
  • 21.
  • 22.
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  • 24.
  • 25.
  • 26. Facilitated diffusionFacilitated diffusion  Allows diffusion of large, membrane insoluble compoundsAllows diffusion of large, membrane insoluble compounds such as sugars and amino acidssuch as sugars and amino acids  Does not require energy (passive)Does not require energy (passive)  Highly SelectiveHighly Selective  Substance binds to membrane- transport proteinSubstance binds to membrane- transport protein  Fully reversible - molecules may enter the cell and leaveFully reversible - molecules may enter the cell and leave the cell through the transport protein.the cell through the transport protein.  Particles move from areas of high concentration to areas ofParticles move from areas of high concentration to areas of low concentration.low concentration.  Movement rate of particles will saturateMovement rate of particles will saturate – Maximum rate limited by number of transportersMaximum rate limited by number of transporters – Once all transporters are operating at 100%, an increase inOnce all transporters are operating at 100%, an increase in concentration will not increase rateconcentration will not increase rate
  • 27.
  • 28.
  • 29. Active TransportActive Transport  Movement across membrane with anMovement across membrane with an energy cost (usually against concentrationenergy cost (usually against concentration  Used to pump specific compounds in orUsed to pump specific compounds in or out of the cellout of the cell  Requires energy to overcome theRequires energy to overcome the concentration gradient or to allow a largeconcentration gradient or to allow a large or charged particle to cross membraneor charged particle to cross membrane  Requires specific integral membraneRequires specific integral membrane proteinsproteins – Can be saturated like facilitated diffusionCan be saturated like facilitated diffusion proteinsproteins – The energy requirement distinguishes activeThe energy requirement distinguishes active transport from facilitated diffusiontransport from facilitated diffusion
  • 30.
  • 31.
  • 32. OsmosisOsmosis  The movement ofThe movement of waterwater acrossacross selectively permeable membranesselectively permeable membranes..  TheThe waterwater moves from amoves from a lowlow concentration areaconcentration area toto highhigh concentration areaconcentration area
  • 33.
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  • 36.
  • 37. Cell membranes are completely permeable to water, therefore, the environment the cell is exposed to can have a dramatic effect on the cell.
  • 38.
  • 39.
  • 40. Hypotonic solutionHypotonic solution  Less soluteLess solute  More waterMore water  Compare to cytoplasm of the cellCompare to cytoplasm of the cell
  • 41. Hypertonic solutionHypertonic solution  More soluteMore solute  Less waterLess water  Compare to cytoplasm of the cellCompare to cytoplasm of the cell
  • 42. Isotonic solutionIsotonic solution  Equal concentration compare toEqual concentration compare to cytoplasm of the cellcytoplasm of the cell
  • 43. Hypertonic Solution: • Solute concentration of solution higher than cell • More dissolved particles outside of cell than inside of cell • Hyper = more (think hyperactive); Tonic = dissolved particles • Water moves out of cell into solution • Cell shrinks
  • 44. Hypotonic Solution: • Solute concentration of solution lower than cell • Less dissolved particles outside of cell than inside of cell • Hypo = less, under (think hypodermic, hypothermia); Tonic = dissolved particles • Water moves into cell from solution • Cell expands (and may burst)
  • 45. Isotonic Solution: Solute concentration of solution equal to that of cell No net water movement
  • 46.
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  • 50.
  • 51.
  • 52. Why the use of excess fertilizer caused wilting in plant?