Microfluidics Corp. Microfluidizers

1. Introducing the new low volume Microfluidizer the LV1 small sample volume (1-20ml), high pressure (30KPSI) and near total sample recovery. Multiple systems scalable from 1 ml to gallons per minute. Perfect for R&D, discovery, discovery exploratory, small clinical studies, pilot scale right through to production scale systems

2. ` The new low voulme 1–20ml Microfluidizer the LV1 To learn more about the LV1 or the differences between the Microfluidizers, homogenizers and the French Press please go to www.microfluidicscorp.com and go to our Webinars Tab or continue with this presentation Bill Travers Manufacturer’s Representative Mobile: 410-746-0488 [email_address] Perfect for Nanoemulsions | Nanodispersions | Liposomes | Cell Disruption | Continuous Crystallization (Replace your obsolete French Press)

4. Discover the Difference: Microfluidizer ® Processors vs. Homogenizers, Sonicators and the French Press

7. Heart of the Technology Fixed-Geometry Interaction Chamber - Microfluidics exclusive - Generates incredibly high shear and impact forces - Precisely engineered microchannels - Repeatable and scalable results - Diamond or ceramic construction More than Machines - Proof of Concept - Process Development - Preventive Maintenance - Safeguard Spares™ - Scaleup consulting - MRT Development

8. Applications and Benefits Pharmaceutical Biotechnology Chemical Energy More efficient catalysts and scaleable processes for fuel cells, batteries, photovoltaics and biofuels Cosmetics Brighter colors and controlled actives delivery for premium and natural lipsticks, hair sprays, clays, etc. Food/Nutraceuticals New ingredient flavors, time-release vitamins and encapsulated odors of nutrients such as Omega-3 Select Applications Proven Benefits Vaccines & adjuvants Improved bioavailability Oncology (injectables) Controlled drug delivery Asthma (inhalables) Sterile filtration Antibiotics Extended shelf life Steroids Continuous processing Select Applications Proven Benefits E. coli High rupture rates Yeast Maximized protein yield Algae Temperature control Mammalian Lower pressures/fewer passes Plant, insect, etc. No contamination Select Applications Proven Benefits Carbon nanotubes Uniform dispersions Inkjet inks Less energy required Coatings & sealants Consistent product quality Fine chemicals Easy to use and clean Polymers & waxes Agrochemicals

9. Our Customers Pharma/Biotech Chemical Food and Nutraceuticals Higher Education Over 150 Machines Sold in 2010 3M | Alcon Labs | Allergan | Aveda | BASF | Baxter | Center for Disease Control | Dana Farber General Mills | Max Planck Institute | MedImmune | Nektar Therapeutics | National Institute of Health PepsiCo. | RiteDose | SAIC | Scripps Research Institute | U.S. Army | U.S. Cosmetics | Yantai Spandex Plus dozens of universities as part of our global Academic Research Collaboration (ARC) program Cosmetic

10. Microfluidizer Technology How It Works

11. Microfluidizer Processor Schematic Inlet Reservoir Intensifier Pump Pressures up to 40,000 psi (276 MPa) Cooling Jacket Outlet Interaction Chamber Product

13. Shear Rates for Various Technologies Agitator Sawtooth Blade Closed Rotor Rotor-Stator Colloid Mill Homogenizer Microfluidizer Processor

14. Particle Size Reduction Vs. High Pressure Homogenizers

18. Before After 15 passes 0 p 1p 2p 3p 4p 5p 7p 10p 15p Process pressure : 18,000 psi (1241 bar) Chamber: F20Y (75 microns) Final product is translucent O/W - Drug Nanoemulsion (Cancer Drug)

19. Median = 0.075  m Median = 14.53  m Before After Emulsion: Oxygen Carrier (Perfluorocarbon) in a Cosmetic Application

20. Liposome Results Particle Size Consistent with emulsions, average particle sizes with Microfluidizer are approximately half the size Uniformity Variability advantages are even more pronounced here – the homogenizer sample contains multiple peaks Did You Know? Microfluidics was originally founded as a liposome producing company Microfluidizer Processor Leading Homogenizer Pass 1 113 nm 268 nm Pass 2 95 nm 228 nm Pass 3 72 nm 183 nm

21. Liposome: Anti-Oxidant – Vitamin C Median = 4.393  m Median = 0.195  m Before After

23. Example – De-agglomeration Unprocessed 2 passes G10Z 30k

25. Customer Success Story: Corixa (now GSK) Vaccine Adjuvant Nanoemulsion Based on these data, Corixa switched from their leading homogenization equipment to a Microfluidics-powered production environment       Critical Quality Criteria Leading Homogenizer Microfluidizer Average Particle Size 185 nm after 15 passes 141 nm after 3 passes Goal: <150 nm Polydispersity 43% above 200 nm 0.5% above 200 nm Goal: <10% above 200 nm Active Concentration 15% loss of actives 1% loss of actives Goal: <2% loss of actives 640 cm 2 17 cm 2 Microfluidics reduced filter area required by over 97%

26. Cell Disruption Vs. Sonicators and the French Press

34. Comparison to Other Mechanical Methods * Now available with the LV1 Microfluidizer processor Microfluidizer Homogenizer Bead Mill Continuous Yes Yes No Scalable Yes Limited Yes Optimal Temp Control Yes Yes No Contamination Free Yes Uncertain No Minimum Volume 1ml* 10 ml 1 ml Constant Shear Rate Yes No No Shear Rate Potential Highest High Medium

35. BEFORE AFTER Process pressure : 18,000 psi (1241 bar) Chamber: H10Z (100 microns) Shear rate: 5.58 X 10 6 s -1 E. Coli Microfluidics users typically rupture >99% of E. coli cells in a single pass

36. Yeast Lysis ( S. Pombe ) Unprocessed 1 pass ~60% lysis 5 passes ~95% lysis 10 passes ~99% lysis Process conditions : 30,000 psi (2070 bar) Chamber: G10Z (87 microns) Shear rate per pass: 6.94 X 10 6 s -1 Microfluidizers are tough on cells (even yeast) and gentle on proteins

40. Algae Cell Disruption Example #1 Process conditions : 1 passes 10,000 psi (690 bar) Chamber: H10Z (100 microns) Shear rate: 4.14 X 10 6 s -1

41. BEFORE AFTER Process conditions : 3 passes 20,000 psi (1380 bar) Chamber: G10Z (87 microns) Shear rate: 6.09 X 10 6 s -1 Algae Cell Disruption Example #2