Jim Hardy discusses the commercial applications of stem cells. He outlines the history of stem cell discoveries and projections for the stem cell market. Some commercial opportunities include tissue sourcing, cell culture materials, tools for cell expansion and characterization, and using stem cells in drug discovery as replacements for primary cells in testing and disease modeling. Challenges include scaling ex vivo cell expansion and understanding tissue-specific developmental pathways well enough for therapeutic applications.
WEBINAR Characterisation of human pluripotent stem cells (ESCs and IPSC) and ...Quality Assistance s.a.
Valérie DEFFONTAINE, R&D Scientist, Quality Assistance
Webinar held on 8th June 2017.
The discovery of human pluripotent stem cells 10 years ago turned the spotlight on the potential of pluripotent stem cells for personalised cell therapy. The scientific interest then quickly shifted towards the use of these cells for safety pharmacology, drug discovery and disease modelling. For all these purposes, in the mid to long term, properly characterised cell banks will be necessary.
The characterisation of embryonic (ESC) and induced pluripotent stem cells (IPSC) used for manufacturing requires the development and validation of analytical methods (e.g. flow cytometry, microscopy, QPCR and bioassays). Cell characterisation includes the testing of cell product identity, determination of impurities, and assessment of biological activity and viability. Among the techniques available, flow cytometry is widely used to assess the expression of cell markers. Our laboratory has developed flow cytometry panels dedicated to the characterisation of extracellular and intracellular markers of ESC and IPSC, and to the detection of cell-related impurities. We proposed a method for the validation of flow cytometry panels according to the recommendations of international guidelines on the validation of analytical methods.
IPSC differentiated into cardiomyocytes and MSC-like cells were also used to test the performance of our flow cytometry panels to accurately monitor the manufacturing process of cell products.
In addition to the technical tips, this webinar aims at presenting a critical view on the use of flow cytometry platform for cell characterisation.
For more information, visit http://www.quality-assistance.com/analytical-services/CBMPs
Development of quality control assays for cell-based medicinal products (ISCT...Quality Assistance s.a.
Dr. Fabian Vandermeers from Quality Assistance spoke on Development of quality control assays for cell-based medicinal products at ISCT 2017 in London.
For more information on this topic, visit: http://www.quality-assistance.com/analytical-services/CBMPs
For more information on our expertise and services, visit: www.quality-assistance.com
Follow us on social media:
LinkedIn: https://www.linkedin.com/company/quality-assistance
Twitter: https://twitter.com/QA_Belgium
Facebook: https://www.facebook.com/QualityAssistanceBelgium
Google +: https://plus.google.com/103676189647965359292
Quality Assistance S.A. is a leading European Contract Research Organisation providing the pharmaceutical industry with all the analytical services required by EMA and FDA regulations for the development and marketing of innovative human medicinal products.
We assist our clients from candidate selection, through non-clinical and clinical studies, to marketing authorisation, using our state-of-the-art, product-dedicated expertise in analytical sciences.
For each customer and each project, we design customised solutions, define analytical protocols, develop and validate specific new analytical methods and perform characterisation, stability, pharmacokinetic, biomarker and immunogenicity studies as well as batch release testing, in order to evaluate the Quality, Safety and Efficacy of the given drugs.
What process is used to obtain cells from bone marrow and normal peripheral blood?
What is the best cell counting and viability method for primary cells?
AllCells, your primary cells research partner, and Nexcelom Bioscience, your cell counting experts, have joined together in an exclusive collaboration to host a free webinar to help educate researchers and present data from their own experiences.
Importance of matricial and cellular microenvironments in in vitro models for...HCS Pharma
There is a 90% failure in clinical trials, due to efficacy and safety issues, which frequently concerns the central nervous system. That points a lack of relevance of preclinical models used upstream. In this frame, the aim of this study was to develop more relevant cerebral in vitro models, by including the matricial and cellular microenvironments, for drug discovery in Parkinson's disease.
HD available on https://hcs-pharma.com/poster---importance-of-matricial-and-cellular-microenvironments-in-in-vitro-models-for-drug-discovery-in-parkinsons-disease/
This document describes a case study of a 37-year-old woman with spinal cord injury who received a transplant of multipotent stem cells derived from human umbilical cord blood. Within 41 days of the transplant, the patient showed improved sensory perception and mobility in her hips and thighs. CT and MRI scans also showed regeneration of the spinal cord at the injury site and some regeneration of the cauda equina below. The results suggest that umbilical cord blood multipotent stem cell transplantation may be an effective treatment for spinal cord injury patients.
1) Researchers studied a novel method for quantifying and tracking macrophage homing into atherosclerotic plaques using superparamagnetic iron oxide nanoparticles (SPIO).
2) They injected SPIO into ApoE-deficient mice with and without proinflammatory cytokines and found higher iron content and more macrophages in plaques of cytokine-treated mice using histology and mass spectrometry.
3) This demonstrates that SPIO allows both quantitative and qualitative assessment of macrophage infiltration and shows infiltration is enhanced by cytokines, suggesting SPIO-enhanced MRI could noninvasively monitor monocyte recruitment dynamics into plaques over time.
1) Researchers studied a novel method for quantifying and tracking macrophage homing into atherosclerotic plaques using superparamagnetic iron oxide nanoparticles (SPIO).
2) They injected SPIO into ApoE-deficient mice, some of which also received proinflammatory cytokines, and tracked iron uptake in plaques over time.
3) Both quantitative iron assays and staining showed greater iron and macrophage presence in plaques of cytokine-treated mice, demonstrating SPIO can monitor macrophage recruitment influenced by inflammation. This noninvasive MRI-based method may help study monocyte involvement in atherosclerosis.
Jim Hardy discusses the commercial applications of stem cells. He outlines the history of stem cell discoveries and projections for the stem cell market. Some commercial opportunities include tissue sourcing, cell culture materials, tools for cell expansion and characterization, and using stem cells in drug discovery as replacements for primary cells in testing and disease modeling. Challenges include scaling ex vivo cell expansion and understanding tissue-specific developmental pathways well enough for therapeutic applications.
WEBINAR Characterisation of human pluripotent stem cells (ESCs and IPSC) and ...Quality Assistance s.a.
Valérie DEFFONTAINE, R&D Scientist, Quality Assistance
Webinar held on 8th June 2017.
The discovery of human pluripotent stem cells 10 years ago turned the spotlight on the potential of pluripotent stem cells for personalised cell therapy. The scientific interest then quickly shifted towards the use of these cells for safety pharmacology, drug discovery and disease modelling. For all these purposes, in the mid to long term, properly characterised cell banks will be necessary.
The characterisation of embryonic (ESC) and induced pluripotent stem cells (IPSC) used for manufacturing requires the development and validation of analytical methods (e.g. flow cytometry, microscopy, QPCR and bioassays). Cell characterisation includes the testing of cell product identity, determination of impurities, and assessment of biological activity and viability. Among the techniques available, flow cytometry is widely used to assess the expression of cell markers. Our laboratory has developed flow cytometry panels dedicated to the characterisation of extracellular and intracellular markers of ESC and IPSC, and to the detection of cell-related impurities. We proposed a method for the validation of flow cytometry panels according to the recommendations of international guidelines on the validation of analytical methods.
IPSC differentiated into cardiomyocytes and MSC-like cells were also used to test the performance of our flow cytometry panels to accurately monitor the manufacturing process of cell products.
In addition to the technical tips, this webinar aims at presenting a critical view on the use of flow cytometry platform for cell characterisation.
For more information, visit http://www.quality-assistance.com/analytical-services/CBMPs
Development of quality control assays for cell-based medicinal products (ISCT...Quality Assistance s.a.
Dr. Fabian Vandermeers from Quality Assistance spoke on Development of quality control assays for cell-based medicinal products at ISCT 2017 in London.
For more information on this topic, visit: http://www.quality-assistance.com/analytical-services/CBMPs
For more information on our expertise and services, visit: www.quality-assistance.com
Follow us on social media:
LinkedIn: https://www.linkedin.com/company/quality-assistance
Twitter: https://twitter.com/QA_Belgium
Facebook: https://www.facebook.com/QualityAssistanceBelgium
Google +: https://plus.google.com/103676189647965359292
Quality Assistance S.A. is a leading European Contract Research Organisation providing the pharmaceutical industry with all the analytical services required by EMA and FDA regulations for the development and marketing of innovative human medicinal products.
We assist our clients from candidate selection, through non-clinical and clinical studies, to marketing authorisation, using our state-of-the-art, product-dedicated expertise in analytical sciences.
For each customer and each project, we design customised solutions, define analytical protocols, develop and validate specific new analytical methods and perform characterisation, stability, pharmacokinetic, biomarker and immunogenicity studies as well as batch release testing, in order to evaluate the Quality, Safety and Efficacy of the given drugs.
What process is used to obtain cells from bone marrow and normal peripheral blood?
What is the best cell counting and viability method for primary cells?
AllCells, your primary cells research partner, and Nexcelom Bioscience, your cell counting experts, have joined together in an exclusive collaboration to host a free webinar to help educate researchers and present data from their own experiences.
Importance of matricial and cellular microenvironments in in vitro models for...HCS Pharma
There is a 90% failure in clinical trials, due to efficacy and safety issues, which frequently concerns the central nervous system. That points a lack of relevance of preclinical models used upstream. In this frame, the aim of this study was to develop more relevant cerebral in vitro models, by including the matricial and cellular microenvironments, for drug discovery in Parkinson's disease.
HD available on https://hcs-pharma.com/poster---importance-of-matricial-and-cellular-microenvironments-in-in-vitro-models-for-drug-discovery-in-parkinsons-disease/
This document describes a case study of a 37-year-old woman with spinal cord injury who received a transplant of multipotent stem cells derived from human umbilical cord blood. Within 41 days of the transplant, the patient showed improved sensory perception and mobility in her hips and thighs. CT and MRI scans also showed regeneration of the spinal cord at the injury site and some regeneration of the cauda equina below. The results suggest that umbilical cord blood multipotent stem cell transplantation may be an effective treatment for spinal cord injury patients.
1) Researchers studied a novel method for quantifying and tracking macrophage homing into atherosclerotic plaques using superparamagnetic iron oxide nanoparticles (SPIO).
2) They injected SPIO into ApoE-deficient mice with and without proinflammatory cytokines and found higher iron content and more macrophages in plaques of cytokine-treated mice using histology and mass spectrometry.
3) This demonstrates that SPIO allows both quantitative and qualitative assessment of macrophage infiltration and shows infiltration is enhanced by cytokines, suggesting SPIO-enhanced MRI could noninvasively monitor monocyte recruitment dynamics into plaques over time.
1) Researchers studied a novel method for quantifying and tracking macrophage homing into atherosclerotic plaques using superparamagnetic iron oxide nanoparticles (SPIO).
2) They injected SPIO into ApoE-deficient mice, some of which also received proinflammatory cytokines, and tracked iron uptake in plaques over time.
3) Both quantitative iron assays and staining showed greater iron and macrophage presence in plaques of cytokine-treated mice, demonstrating SPIO can monitor macrophage recruitment influenced by inflammation. This noninvasive MRI-based method may help study monocyte involvement in atherosclerosis.
Cell Storage Best Practices: Techniques to Ensure Greater Research Resultsallcells
Featuring: Cryopreservation Specialist, Michael George
This webinar will introduce cell storage equipment and techniques in order to provide the most up-to-date trends in research today. There will be special emphasis on human primary cell storage, cryopreservation, and transportation.
Topics to be covered in the webinar:
• Background on life science storage methods
• Advantages/disadvantages of storage equipment
• What not to do (common mistakes/misconceptions)
• Interview with James Lee, Associate Director of Production, AllCells
• Q&A
This document discusses primary cells and their use in biomedical research. Primary cells are isolated directly from tissues and have important advantages over cell lines, including greater biological relevance. Primary cells are used in applications such as biomarker research, drug development, immunotherapeutics, and tissue engineering. The document reviews techniques for working with primary cells, including isolation, culture, differentiation, and characterization using methods like flow cytometry and immunoassays. Primary cells provide a more physiologically relevant model for research compared to cell lines.
The exosomes isolated from serum of patients with systemic sclerosis contained higher levels of profibrotic miRNAs and lower levels of antifibrotic miRNAs compared to exosomes from healthy individuals. When applied to normal dermal fibroblasts in culture, the scleroderma patient exosomes stimulated the expression of profibrotic genes, mimicking the phenotype of scleroderma fibroblasts. This effect was partially reduced when the exosomes were pre-treated to degrade either the RNA or protein content, indicating both components contribute to the exosomes' ability to induce a profibrotic phenotype in target cells and suggesting a potential mechanism for the transmission and progression of fibrosis in scleroderma.
This document provides an overview of stem cell technology and research. It discusses the history and types of stem cells, including embryonic and adult stem cells. Embryonic stem cells are pluripotent and can form virtually any cell type, while adult stem cells are multipotent and more limited in what cells they can become. The document also reviews potential applications of stem cell therapy in treating conditions like diabetes, heart disease, and arthritis. However, ethical issues around the use of embryonic stem cells remain controversial.
The document summarizes Kirkstall's work developing physiologically relevant in vitro models using their Quasi Vivo perfusion bioreactor systems. It describes several projects utilizing different cell types like hepatocytes, endothelial cells, neurons that demonstrate improved cell viability, proliferation and function under flow compared to static culture. This includes more accurate liver drug toxicity responses and formation of 3D tissue-like structures for the blood brain barrier, gut, skin and cardiac models. The document also acknowledges collaborations with various academic partners on these projects.
This document describes a protocol for differentiating mouse induced pluripotent stem cells (miPSCs) into oligodendrocyte progenitor cells (OPCs). The protocol utilizes a defined, stepwise differentiation process over 8 days to generate high yields of OPCs from three different miPSC lines. The OPCs produced from this protocol are capable of further differentiation into mature, myelinating oligodendrocytes both in vitro and in vivo, making them a potentially useful cell source for modeling demyelinating disorders and developing cell-based therapies.
Utilizing Cell Imaging Technology to Shorten Timelines and Reduce Development Resources by Earlier Identification of High Producing Recombinant CHO Cell Lines
This document describes several approaches for studying the role of the Cripto protein in muscle stem cell regeneration:
1) Cripto loss-of-function and gain-of-function mouse models to understand how changes in Cripto expression impact muscle stem cells.
2) Using mouse models of acute muscle damage to study how Cripto influences mobilization of muscle stem cells.
3) High-throughput screening to identify new regulators of muscle stem cells by analyzing gene expression changes from Cripto loss-of-function cells.
4) Developing recombinant Cripto protein and pharmacological delivery methods to promote stem cell-based muscle regeneration.
AgeX Presentation: Ending Age-related Diseases, 7-12-18
Comment: it is an assumption that the repression of telomerase "protects" against cancer. The opposite is the case: the repression of telomerase leads to shorter telomeres and thus increases the risk for cancer.
"From ancient times, three powerful forces have converged to shape our view of life in this world. The first such force, largely unique to the human species, is our profound capacity to love one another. The second is death - the greatest enemy of love.
The stark reality that our lives and loves will end in shipwreck and extinction threatens all that we hold dear. Love and death are entirely incompatible.
But the human brain is resilient, therefore virtually every known culture in the history of mankind has devised strategies to escape the labyrinth of the despair of death.
Regardless of the ethereal nature of these escape plans, they provided the bases of the third force: hope.
And so, the hope for immortality was the direct consequence of the titanic collusion of the forces of human love and death." —Michael D. West
“I wouldn’t haven taken LSD if it hadn’t been exposed and people hadn’t made experiments, but when I took it, that first time, I saw immortality! I saw timelessness, and I saw that, for me – and I’ve shared my experience with a few other people who happened to have experienced the same substance – and I said: „Didn’t you really feel that that key to immortality was being truthful? And just letting go of your baloney and that in fact, it was that ego that was telling you you’re gonna die, which was the real culprit!” ―Timothy Leary & Robert Anton Wilson: Immortality, Space, & the Future (New Horizons #3)
"If I still remember that all realities are neurological constructs and relative to the observer, I am nonetheless committed now to one reality above all alternatives: the reality of Jesus and Buddha, in which reverence for life is the supreme imperative." —Robert Anton Wilson
"Now let's turn our attention to the future. It's interesting to ponder on just how far this will take us. Will we one day eliminate the biology of aging the way we've eliminated diseases like say smallpox?
Will we one day consider aging itself a disease? Is immortality transfer possible for the whole human body?
In times past, scientists would loudly criticize such claims as dangerous fantasy, science fiction and certainly not science fact. They appoint it to the second law of thermodynamics and say that all system run down over time, it's a principle of nature.
But life isn't a closed system as defined by the second law.
The cells that reside in our bodies have no dead ancestors, they will die in you and I for the first time in billions of years. It should therefor not come as a surprise that we naturally recoil at the thought of death." —Michael D. West
Aging is programmed by SATURN-KRONOS to create the illusions of time and death: to oppress mankind into matter.
Isolation of an adult blood derived progenitor cell population capable of dif...lifextechnologies
This research paper describes the isolation of a novel human cell population from peripheral blood termed the synergetic cell population (SCP). The SCP contains multipotent progenitor cells that are capable of differentiating into angiogenic, neural, and myocardial cell lineages when exposed to defined culture conditions. Specifically, the SCP gives rise to angiogenic cell precursors, neural cell precursors, and myocardial cell precursors that express markers and exhibit functions characteristic of these lineages. The isolation method provides considerable quantities of lineage-specific precursor cells, making the SCP a potential source of autologous cells for the treatment of various diseases.
This study investigated the proliferation characteristics and tumorigenic potential of oval cells, the proposed stem cells of the liver. Male rats were fed a choline-deficient diet to induce oval cells, which were then isolated and cultured over 100 passages over 2 years. The oval cells were analyzed using flow cytometry and markers to observe their immunophenotype, proliferation ability, and differentiation potential. When xenografted into immunodeficient mice, the oval cells did not spontaneously transform into cancer-initiating cells. This suggests hepatic oval cells may serve as a safe cell source for future cell therapy and studying liver carcinogenesis.
Induced pluripotent stem cells (iPSCs) are adult cells that have been genetically reprogrammed to an embryonic stem cell-like state. iPSCs have the potential to be used to generate cells and tissues for medical therapies without the ethical issues associated with embryonic stem cells. While promising, iPSCs still face obstacles like the risk of forming tumors that must be addressed before they can be used to treat diseases and conditions.
1. A study investigated the anti-cancer properties of camel urine in vitro. Camel urine was found to inhibit cell proliferation and induce apoptosis in various human cancer cell lines through the intrinsic apoptosis pathway.
2. Camel urine downregulated several cancer-promoting proteins and upregulated a cyclin-dependent kinase inhibitor. It also had no cytotoxic effects on immune cells and stimulated immune responses by inducing an anti-tumor cytokine and inhibiting pro-tumor cytokines.
3. The results suggest that components in camel urine have specific and efficient anti-cancer effects in vitro as well as immune-modulating properties, providing a potential basis for the ethnopharmacological use of camel urine to treat cancer.
In-vitro Cell culture is developing rapidly with the primary goal being to model human physiology in a realistic way that predicts clinical results. This presentation shares the strategy being employed by Kirkstall Ltd to provide a practical solution to the challenges of creating an organ on-a-chip.
Cytes Bio Technologies has been supplying for several years from Human tissues to Human Kupffer Cells. At Cytes Bio Technologies, we have tissue for research according to your requirements. All our tissues meets with all ethical standards and appropriate regulations for the acquisition, handling as well as supply of human biological products for research. Get in touch with us soon.
esibio General Sales presentation August updateShaun Teacher
This document provides information about Biotime's products and services related to 3D cell culture and delivery. It discusses HyStem hydrogels which are 3D cell culture and delivery vehicles. It provides details on the modular nature of HyStem and its components. It also highlights some success stories using HyStem for cell delivery in neural stem cells, islets, hepatocytes, and cardiospheres. Additionally, it discusses using HyStem for cell culture of organoids, fibroblasts, and cardiomyocytes. It also includes information on ESI Bio's RNA reprogramming system and their lines of hES cells, progenitor cells, small molecules, and antibodies.
Cellular and gene therapies are now on the cusp of realizing the early promise of treating, and perhaps curing diseases that previously had little or no available therapeutic options. However, the journey from the laboratory to the clinic has been bumpy, with a history of disappointments, especially in the early 1990's where gene therapy had received much hype and publicity.
This document discusses menstrual blood stem cells (MenSCs) as a potential source for regenerative medicine. MenSCs can be easily isolated from menstrual blood and expanded. They express stem cell markers and can differentiate into multiple cell types, including cartilage, bone, fat, neurons and heart muscle cells. MenSCs have a fast doubling time and advantages over other stem cell sources for potential clinical applications. Further research is still needed to fully evaluate their therapeutic potential.
Novel Way to Isolate Adipose Derive Mesenchymal Stem Cells & Its Future Clini...IOSR Journals
Abstract: Adipose-derived stem cells (ADSCs), were isolated from discarded human fat tissue, obtained from csection with our recently modified methods, in Stem Cell & Regenerative Medicine Lab, VSBT. Here we develop
two methods to isolate Adipose derived mesenchymal stem cells with enzyme digestion and use of
phosphatidylcholine and deoxycholate. Surface protein expression was analyzed by flow cytometry to
characterize the cell phenotype. The multi-lineage potential of ADSCs was testified by differentiating cells with
adipogenic inducer. ADSCs can be cultured in vitro for up to one month without passage. Also, the flow
cytometry analysis showed that ADSCs expressed high levels of stem cell related surface marker CD105.
ADSCs have strong proliferation ability, maintain their phenotypes, and have stronger multi-differentiation
potential. The molecular basis of ADSC differentiation was studied using bioinformatics tools with an aim to
identify the key proteins involved in differentiation, such that they could be used as potential targets for drug
development for the treatment of obesity. The key proteins involved were found to be PPARG and C/EBPα. The
structures of the proteins were retrieved from MMDB (Molecular Modelling Database) and PDB (Protein Data
Bank) respectively. Key Words: Adipose-derived stem cells, Mesenchymal stem cells, Enzyme digestion, Phosphatidylcholine, Deoxycholate, PPARG, C/EBPα, etc.
Genetic screening of CRISPR edited human-derived induced pluripotent stem cells was conducted to create a model of Costello syndrome. CRISPR was used to induce mutations in the HRAS gene of iPSCs. Of 12 samples screened, 2 were found to have a heterozygous mutation and 1 had a homozygous mutation, supporting that CRISPR can be used for gene editing in iPSCs. The mutated iPSCs will be differentiated into cardiomyocytes to model cardiac abnormalities in Costello syndrome.
Martin Pera stem cells and the future of medicineigorod
This document discusses stem cell research and regenerative medicine. It begins by defining regenerative medicine and stem cells. It describes different types of stem cells including tissue stem cells and embryonic stem cells. It discusses some clinical uses of tissue stem cells and limitations. It then covers the discovery of human embryonic stem cells in 1998 and their potential uses and challenges. The rest of the document discusses various stem cell research projects at USC including using stem cells to study disease, induced pluripotent stem cells, and stem cell-based therapies for conditions like macular degeneration and HIV/AIDS.
Cell Storage Best Practices: Techniques to Ensure Greater Research Resultsallcells
Featuring: Cryopreservation Specialist, Michael George
This webinar will introduce cell storage equipment and techniques in order to provide the most up-to-date trends in research today. There will be special emphasis on human primary cell storage, cryopreservation, and transportation.
Topics to be covered in the webinar:
• Background on life science storage methods
• Advantages/disadvantages of storage equipment
• What not to do (common mistakes/misconceptions)
• Interview with James Lee, Associate Director of Production, AllCells
• Q&A
This document discusses primary cells and their use in biomedical research. Primary cells are isolated directly from tissues and have important advantages over cell lines, including greater biological relevance. Primary cells are used in applications such as biomarker research, drug development, immunotherapeutics, and tissue engineering. The document reviews techniques for working with primary cells, including isolation, culture, differentiation, and characterization using methods like flow cytometry and immunoassays. Primary cells provide a more physiologically relevant model for research compared to cell lines.
The exosomes isolated from serum of patients with systemic sclerosis contained higher levels of profibrotic miRNAs and lower levels of antifibrotic miRNAs compared to exosomes from healthy individuals. When applied to normal dermal fibroblasts in culture, the scleroderma patient exosomes stimulated the expression of profibrotic genes, mimicking the phenotype of scleroderma fibroblasts. This effect was partially reduced when the exosomes were pre-treated to degrade either the RNA or protein content, indicating both components contribute to the exosomes' ability to induce a profibrotic phenotype in target cells and suggesting a potential mechanism for the transmission and progression of fibrosis in scleroderma.
This document provides an overview of stem cell technology and research. It discusses the history and types of stem cells, including embryonic and adult stem cells. Embryonic stem cells are pluripotent and can form virtually any cell type, while adult stem cells are multipotent and more limited in what cells they can become. The document also reviews potential applications of stem cell therapy in treating conditions like diabetes, heart disease, and arthritis. However, ethical issues around the use of embryonic stem cells remain controversial.
The document summarizes Kirkstall's work developing physiologically relevant in vitro models using their Quasi Vivo perfusion bioreactor systems. It describes several projects utilizing different cell types like hepatocytes, endothelial cells, neurons that demonstrate improved cell viability, proliferation and function under flow compared to static culture. This includes more accurate liver drug toxicity responses and formation of 3D tissue-like structures for the blood brain barrier, gut, skin and cardiac models. The document also acknowledges collaborations with various academic partners on these projects.
This document describes a protocol for differentiating mouse induced pluripotent stem cells (miPSCs) into oligodendrocyte progenitor cells (OPCs). The protocol utilizes a defined, stepwise differentiation process over 8 days to generate high yields of OPCs from three different miPSC lines. The OPCs produced from this protocol are capable of further differentiation into mature, myelinating oligodendrocytes both in vitro and in vivo, making them a potentially useful cell source for modeling demyelinating disorders and developing cell-based therapies.
Utilizing Cell Imaging Technology to Shorten Timelines and Reduce Development Resources by Earlier Identification of High Producing Recombinant CHO Cell Lines
This document describes several approaches for studying the role of the Cripto protein in muscle stem cell regeneration:
1) Cripto loss-of-function and gain-of-function mouse models to understand how changes in Cripto expression impact muscle stem cells.
2) Using mouse models of acute muscle damage to study how Cripto influences mobilization of muscle stem cells.
3) High-throughput screening to identify new regulators of muscle stem cells by analyzing gene expression changes from Cripto loss-of-function cells.
4) Developing recombinant Cripto protein and pharmacological delivery methods to promote stem cell-based muscle regeneration.
AgeX Presentation: Ending Age-related Diseases, 7-12-18
Comment: it is an assumption that the repression of telomerase "protects" against cancer. The opposite is the case: the repression of telomerase leads to shorter telomeres and thus increases the risk for cancer.
"From ancient times, three powerful forces have converged to shape our view of life in this world. The first such force, largely unique to the human species, is our profound capacity to love one another. The second is death - the greatest enemy of love.
The stark reality that our lives and loves will end in shipwreck and extinction threatens all that we hold dear. Love and death are entirely incompatible.
But the human brain is resilient, therefore virtually every known culture in the history of mankind has devised strategies to escape the labyrinth of the despair of death.
Regardless of the ethereal nature of these escape plans, they provided the bases of the third force: hope.
And so, the hope for immortality was the direct consequence of the titanic collusion of the forces of human love and death." —Michael D. West
“I wouldn’t haven taken LSD if it hadn’t been exposed and people hadn’t made experiments, but when I took it, that first time, I saw immortality! I saw timelessness, and I saw that, for me – and I’ve shared my experience with a few other people who happened to have experienced the same substance – and I said: „Didn’t you really feel that that key to immortality was being truthful? And just letting go of your baloney and that in fact, it was that ego that was telling you you’re gonna die, which was the real culprit!” ―Timothy Leary & Robert Anton Wilson: Immortality, Space, & the Future (New Horizons #3)
"If I still remember that all realities are neurological constructs and relative to the observer, I am nonetheless committed now to one reality above all alternatives: the reality of Jesus and Buddha, in which reverence for life is the supreme imperative." —Robert Anton Wilson
"Now let's turn our attention to the future. It's interesting to ponder on just how far this will take us. Will we one day eliminate the biology of aging the way we've eliminated diseases like say smallpox?
Will we one day consider aging itself a disease? Is immortality transfer possible for the whole human body?
In times past, scientists would loudly criticize such claims as dangerous fantasy, science fiction and certainly not science fact. They appoint it to the second law of thermodynamics and say that all system run down over time, it's a principle of nature.
But life isn't a closed system as defined by the second law.
The cells that reside in our bodies have no dead ancestors, they will die in you and I for the first time in billions of years. It should therefor not come as a surprise that we naturally recoil at the thought of death." —Michael D. West
Aging is programmed by SATURN-KRONOS to create the illusions of time and death: to oppress mankind into matter.
Isolation of an adult blood derived progenitor cell population capable of dif...lifextechnologies
This research paper describes the isolation of a novel human cell population from peripheral blood termed the synergetic cell population (SCP). The SCP contains multipotent progenitor cells that are capable of differentiating into angiogenic, neural, and myocardial cell lineages when exposed to defined culture conditions. Specifically, the SCP gives rise to angiogenic cell precursors, neural cell precursors, and myocardial cell precursors that express markers and exhibit functions characteristic of these lineages. The isolation method provides considerable quantities of lineage-specific precursor cells, making the SCP a potential source of autologous cells for the treatment of various diseases.
This study investigated the proliferation characteristics and tumorigenic potential of oval cells, the proposed stem cells of the liver. Male rats were fed a choline-deficient diet to induce oval cells, which were then isolated and cultured over 100 passages over 2 years. The oval cells were analyzed using flow cytometry and markers to observe their immunophenotype, proliferation ability, and differentiation potential. When xenografted into immunodeficient mice, the oval cells did not spontaneously transform into cancer-initiating cells. This suggests hepatic oval cells may serve as a safe cell source for future cell therapy and studying liver carcinogenesis.
Induced pluripotent stem cells (iPSCs) are adult cells that have been genetically reprogrammed to an embryonic stem cell-like state. iPSCs have the potential to be used to generate cells and tissues for medical therapies without the ethical issues associated with embryonic stem cells. While promising, iPSCs still face obstacles like the risk of forming tumors that must be addressed before they can be used to treat diseases and conditions.
1. A study investigated the anti-cancer properties of camel urine in vitro. Camel urine was found to inhibit cell proliferation and induce apoptosis in various human cancer cell lines through the intrinsic apoptosis pathway.
2. Camel urine downregulated several cancer-promoting proteins and upregulated a cyclin-dependent kinase inhibitor. It also had no cytotoxic effects on immune cells and stimulated immune responses by inducing an anti-tumor cytokine and inhibiting pro-tumor cytokines.
3. The results suggest that components in camel urine have specific and efficient anti-cancer effects in vitro as well as immune-modulating properties, providing a potential basis for the ethnopharmacological use of camel urine to treat cancer.
In-vitro Cell culture is developing rapidly with the primary goal being to model human physiology in a realistic way that predicts clinical results. This presentation shares the strategy being employed by Kirkstall Ltd to provide a practical solution to the challenges of creating an organ on-a-chip.
Cytes Bio Technologies has been supplying for several years from Human tissues to Human Kupffer Cells. At Cytes Bio Technologies, we have tissue for research according to your requirements. All our tissues meets with all ethical standards and appropriate regulations for the acquisition, handling as well as supply of human biological products for research. Get in touch with us soon.
esibio General Sales presentation August updateShaun Teacher
This document provides information about Biotime's products and services related to 3D cell culture and delivery. It discusses HyStem hydrogels which are 3D cell culture and delivery vehicles. It provides details on the modular nature of HyStem and its components. It also highlights some success stories using HyStem for cell delivery in neural stem cells, islets, hepatocytes, and cardiospheres. Additionally, it discusses using HyStem for cell culture of organoids, fibroblasts, and cardiomyocytes. It also includes information on ESI Bio's RNA reprogramming system and their lines of hES cells, progenitor cells, small molecules, and antibodies.
Cellular and gene therapies are now on the cusp of realizing the early promise of treating, and perhaps curing diseases that previously had little or no available therapeutic options. However, the journey from the laboratory to the clinic has been bumpy, with a history of disappointments, especially in the early 1990's where gene therapy had received much hype and publicity.
This document discusses menstrual blood stem cells (MenSCs) as a potential source for regenerative medicine. MenSCs can be easily isolated from menstrual blood and expanded. They express stem cell markers and can differentiate into multiple cell types, including cartilage, bone, fat, neurons and heart muscle cells. MenSCs have a fast doubling time and advantages over other stem cell sources for potential clinical applications. Further research is still needed to fully evaluate their therapeutic potential.
Novel Way to Isolate Adipose Derive Mesenchymal Stem Cells & Its Future Clini...IOSR Journals
Abstract: Adipose-derived stem cells (ADSCs), were isolated from discarded human fat tissue, obtained from csection with our recently modified methods, in Stem Cell & Regenerative Medicine Lab, VSBT. Here we develop
two methods to isolate Adipose derived mesenchymal stem cells with enzyme digestion and use of
phosphatidylcholine and deoxycholate. Surface protein expression was analyzed by flow cytometry to
characterize the cell phenotype. The multi-lineage potential of ADSCs was testified by differentiating cells with
adipogenic inducer. ADSCs can be cultured in vitro for up to one month without passage. Also, the flow
cytometry analysis showed that ADSCs expressed high levels of stem cell related surface marker CD105.
ADSCs have strong proliferation ability, maintain their phenotypes, and have stronger multi-differentiation
potential. The molecular basis of ADSC differentiation was studied using bioinformatics tools with an aim to
identify the key proteins involved in differentiation, such that they could be used as potential targets for drug
development for the treatment of obesity. The key proteins involved were found to be PPARG and C/EBPα. The
structures of the proteins were retrieved from MMDB (Molecular Modelling Database) and PDB (Protein Data
Bank) respectively. Key Words: Adipose-derived stem cells, Mesenchymal stem cells, Enzyme digestion, Phosphatidylcholine, Deoxycholate, PPARG, C/EBPα, etc.
Genetic screening of CRISPR edited human-derived induced pluripotent stem cells was conducted to create a model of Costello syndrome. CRISPR was used to induce mutations in the HRAS gene of iPSCs. Of 12 samples screened, 2 were found to have a heterozygous mutation and 1 had a homozygous mutation, supporting that CRISPR can be used for gene editing in iPSCs. The mutated iPSCs will be differentiated into cardiomyocytes to model cardiac abnormalities in Costello syndrome.
Martin Pera stem cells and the future of medicineigorod
This document discusses stem cell research and regenerative medicine. It begins by defining regenerative medicine and stem cells. It describes different types of stem cells including tissue stem cells and embryonic stem cells. It discusses some clinical uses of tissue stem cells and limitations. It then covers the discovery of human embryonic stem cells in 1998 and their potential uses and challenges. The rest of the document discusses various stem cell research projects at USC including using stem cells to study disease, induced pluripotent stem cells, and stem cell-based therapies for conditions like macular degeneration and HIV/AIDS.
1. Flow cytometry can be used to identify and characterize stem cells using cell surface markers. Hematopoietic stem cells are one of the best characterized stem cells and can be identified using markers like CD34 and CD38.
2. Cancer stem cells have also been identified in several cancers including cancers of the brain, breast, colon, and prostate. Colon cancer stem cells can be identified as the CD133+CD44+ population and have an increased expression of CD66c.
3. Silencing of the CD66c marker in colon cancer cell lines results in decreased proliferation and tumorigenicity, demonstrating its importance in identifying the colon cancer stem cell population.
This document discusses stem cell niches and their therapeutic applications. It defines a stem cell niche as the microenvironment where stem cells reside, and notes that niches consist of niche cells, stem cells, signals and extracellular matrix that regulate stem cell behavior. Different types of niches are described for blood, cartilage, bone, neural and skin stem cells. The roles of various signaling pathways in maintaining the stem cell niche are also outlined. Finally, the document discusses current and potential future therapeutic applications of stem cells for treating various diseases.
Stem Cells in A New Era of Cell based Therapies - Creative BiolabsCreative-Biolabs
This document discusses stem cells and their applications in cell-based therapies. It defines stem cells as having endless self-renewal and the ability to differentiate into any cell type. Stem cells are classified by potency and origin, and can be embryonic, adult, or induced. The document outlines how different stem cell types like mesenchymal stem cells can treat diseases by regenerating tissues. Current stem cell therapy research aims to treat cancer, autoimmune diseases, and genetic disorders by using stem cells as drug delivery vehicles or for immunotherapy.
International Journal of Stem Cell Research and Transplantation (IJST) is an international, Open Access, peer-reviewed journal, which mainly focuses, on the advancements made in the field of cell biology, specifically in the field of Stem Cells.
International Journal of Stem Cell Research and Transplantation (IJST) is a peer-reviewed journal, and is dedicated to providing information with respect to the latest advancements that are being upgraded in our everyday life with respect to the application of Stem cells.
International Journal of Stem Cell Research and Transplantation (IJST) ISSN:2328-3548, is a free, Open Access, Peer-reviewed, exclusive online journal covering areas of Stem cell research, translational work and Clinical studies in the specialty of Stem Cells and Transplantation including allied specialties relevant to the core subject, which is dedicated in publishing high quality manuscripts.
Stem Cell Technology and its Clinical ApplicationDr. Barkha Gupta
Dr. Barkha Gupta has been teaching Veterinary Biochemistry as well as clinical physiology at CVAS, Udaipur and PGIVER, Jaipur. She has earlier served in various capacities in the Department of Animal Husbandry, Govt. of Rajasthan. She has several publications and awards to her credit. She is the PI of M-RAJUVAS Android Educational Mobile Application for Veterinary and Animal Sciences and Kiosk Information System for Farmers/Livestock Owners. Dr. Gupta is also IFBA Certified Professional.
The document discusses different types of cell cultures, including primary cultures derived from animal tissue, continuous cultures comprised of cell lines, and normal diploid cells with a finite lifespan. It describes common cell lines used in research, including HeLa cells from cervical carcinoma and CHO cells from hamster ovary. The key components of maintaining cell cultures are discussed, such as temperature, atmosphere, sterile conditions, culture medium, and growth equipment. Tasks demonstrate observing cell morphology, quantifying cell density using a hemocytometer, and counting chromosomes in CHO cells, which are shown to have an aneuploid number due to long-term cultivation.
Osteoblasts remotely supply lung tumors with cancer-promoting SiglecFhigh neu...Gul Muneer
Osteoblasts remotely supply lung tumors with cancer-promoting SiglecFhigh neutrophils. Lung tumors disrupt bone homeostasis and increase osteoblast activity and bone formation. Osteoblasts amplify tumor-associated SiglecFhigh neutrophils that promote tumor growth through angiogenesis, immunosuppression and other mechanisms. Serum from tumor-bearing mice increases osteoblast activity through elevated sRAGE, which stimulates neutrophil maturation. SiglecFhigh neutrophils correlate with poor survival in lung cancer patients. Therefore, lung tumors communicate with bone through factors like sRAGE to modulate osteoblasts and promote neutrophil-driven tumor progression.
This document summarizes the potential of human embryonic stem cell research and Advanced Cell Technology's role in developing therapies from this research. It discusses how embryonic and adult stem cells differ, ACT's proprietary technology platforms and human therapeutic programs in retinal pigment epithelium cells, hemangioblast cells, and dermal cells. Key milestones are outlined such as deriving stem cell lines, developing high-throughput differentiation techniques, establishing GMP manufacturing facilities, preclinical studies showing rescued vision in rats, and plans for clinical trials and partnerships. The goal is to translate stem cell science into therapies for major diseases.
Achieving High Yields in Scalable Xeno Free Culture Formats with Mesenchymal ...Merck Life Sciences
Watch the presentation of this webinar here: https://bit.ly/3ryE5ST
Optimize your mesenchymal stem cell growth. Join our webinar to learn more about our GMP-compliant xeno free media formulation that supports high performance expansions and compatibility with scalable xeno free manufacturing conditions.
Optimizing ex vivo cell expansion processes in preparation for clinical use is a critical step in cell therapy manufacturing. Given the curative and lifesaving impacts these therapies can have on patients, overcoming roadblocks with scalability and supply chain, using high quality raw materials are essential for therapeutic access.
The GMP-compliant Stemline® XF MSC Medium and cocktail promotes expansion of human mesenchymal stromal/stem cells (hMSCs) to high densities while maintaining cell identity and quality. This product was designed for derivation and expansion of MSCs using xeno free conditions in planar and microcarrier-based culture platforms, easing the transfer between research, clinical, and manufacturing scale cultures.
In this webinar, you will:
• Explore the current landscape and future trends of cell culture media for adult mesenchymal stem cells
• Discover ways to derive MSC's from Bone Marrow in Xeno Free conditions from static to microcarrier-based suspension culture platforms.
• Learn how Stemline® XF MSC Media provides robust performance and reduces scalability roadblocks
Presented by: Kathleen Ongena, Ph.D., Head of Customer Applications and Mark Ventresco, Cell Therapy Product Manager
Achieving High Yields in Scalable Xeno Free Culture Formats with Mesenchymal ...MilliporeSigma
Watch the presentation of this webinar here: https://bit.ly/3ryE5ST
Optimize your mesenchymal stem cell growth. Join our webinar to learn more about our GMP-compliant xeno free media formulation that supports high performance expansions and compatibility with scalable xeno free manufacturing conditions.
Optimizing ex vivo cell expansion processes in preparation for clinical use is a critical step in cell therapy manufacturing. Given the curative and lifesaving impacts these therapies can have on patients, overcoming roadblocks with scalability and supply chain, using high quality raw materials are essential for therapeutic access.
The GMP-compliant Stemline® XF MSC Medium and cocktail promotes expansion of human mesenchymal stromal/stem cells (hMSCs) to high densities while maintaining cell identity and quality. This product was designed for derivation and expansion of MSCs using xeno free conditions in planar and microcarrier-based culture platforms, easing the transfer between research, clinical, and manufacturing scale cultures.
In this webinar, you will:
• Explore the current landscape and future trends of cell culture media for adult mesenchymal stem cells
• Discover ways to derive MSC's from Bone Marrow in Xeno Free conditions from static to microcarrier-based suspension culture platforms.
• Learn how Stemline® XF MSC Media provides robust performance and reduces scalability roadblocks
Presented by: Kathleen Ongena, Ph.D., Head of Customer Applications and Mark Ventresco, Cell Therapy Product Manager
Cell Therapy Industry News Round-Up - September 13, 2022 [BioInformant]BioInformant
The document discusses several topics related to stem cells and regenerative medicine:
1) It provides an overview of 10 scientists nominated for a $100,000 stem cell research grant to be awarded in October.
2) It describes a new collaboration between RoosterBio and AGC Biologics to accelerate manufacturing of cell and exosome therapies.
3) It announces that Steakholder Foods will begin developing cultured pork products using induced pluripotent stem cells.
This document discusses stem cells and their therapeutic applications. It begins by explaining how the early embryo develops from a single cell through cell division and differentiation. It then defines different types of stem cells based on their potency. The document presents two case studies of using stem cells therapeutically: one for treating Stargardt's macular degeneration by injecting retinal stem cells, and one for treating leukemia by harvesting a patient's stem cells and reintroducing them after chemotherapy. Finally, it discusses the sources of stem cells including embryonic, adult, and induced pluripotent stem cells as well as the ongoing ethical debate around stem cell research.
Organs-on-chips (OoCs) are systems containing engineered or natural miniature tissues grown inside microfluidic chips. To better mimic human physiology, the chips are designed to control cell microenvironments and maintain tissue-specific functions. Combining advances in tissue engineering and microfabrication, OoCs have gained interest as a next-generation experimental platform to investigate human pathophysiology and the effect of therapeutics in the body. There are as many examples of OoCs as there are applications, making it difficult for new researchers to understand what makes one OoC more suited to an application than another.
Cancer is diagnosed in about 1 in 250 men and 1 in 300 women annually according to the WHO. Cancer is clonal in origin and has six hallmarks including immortality, producing growth signals, overriding stop signals, resisting cell death, inducing angiogenesis, and causing metastasis. Treatments include radiotherapy, chemotherapy, hormone therapy, cytokines, monoclonal antibodies, and gene therapy. Induced pluripotent stem cells (iPSCs) were first derived from mouse cells in 2006 and human cells in 2007, earning the discoverers the Nobel Prize. iPSCs can differentiate into many cell types and are useful for modeling diseases, developing immunotherapies and cancer treatments, and studying mechanisms of disease. However, obstacles remain regarding
Advances in Stem Cell Therapies: Still Experimental, or Ready to Go?DrDeAndrea
1) A stem cell researcher in Japan claimed a major discovery by turning adult blood cells into stem cells quickly through acid exposure, but her work is under investigation.
2) Stem cell therapies are generally considered safer when using a patient's own cells, and regulations may be prohibiting doctors from trying to cure patients with autologous stem cell treatments.
3) Pharmaceutical companies could feel threatened by stem cell therapies becoming as common as drugs, representing mobile pharmacies of healing factors, within 20 years according to one scientist.
Similar to Commercial Applications of Stem Cells: NIH Lecture 06 Oct 2010 (20)
The document provides an overview of aseptic practices and microbiology basics. It discusses:
- Definitions of aseptic, sterile, and related terms.
- A brief history of aseptic techniques and milestones like the development of the microscope, iodine, and HEPA filters.
- The importance of microbiology in contamination control and why microbes are studied. It describes common types of microbes like bacteria, fungi, and viruses.
- Sources of contamination and methods for contamination control including cleaning/disinfection, hygiene practices, facilities design with HEPA filters and air flow, and the four pillars of aseptic techniques.
- Personal responsibility in contamination prevention through practices like hand
Machine pulsatile perfusion is a novel technique for isolating stem cells from the entire afterbirth, including placenta and umbilical cord, which results in a 2-5x higher yield of CD34+ stem cells compared to cord blood alone. The technique mimics the human heart and gently extracts stem cells using perfusate chemistry matched to normal body conditions. Studies show afterbirth-derived stem cells isolated using this method have similar performance to cord blood stem cells for blood and immune cell production and it is being explored as a new source of stem cells for transplantation therapies.
The new LV1 Microfluidizer processor allows for high pressure processing of small sample volumes between 1-20ml with near total sample recovery. It is well-suited for applications in research and development, discovery, exploratory studies, and small clinical studies due to its scalability and ability to process small volumes. The LV1 provides consistent shear rates and particle size reduction performance comparable to larger Microfluidizer systems.
This document contains a collection of amusing quotes that reveal unintentionally funny or nonsensical statements. Some examples include Miss Alabama saying she would not want to live forever because if people were meant to live forever then they would, and Mariah Carey saying she wishes she could be as skinny as starving children but without the flies and death.
Failing a test can feel embarrassing, but it's important to remember that one test result does not define you. Maintain confidence by focusing on all of your strengths and accomplishments rather than one setback. After the test, talk to your teacher about what areas you can improve in and make a plan to study those topics more before the next assessment.
The document discusses new technologies for analyzing particulates in therapeutic protein formulations. It describes the Fluid Imaging FlowCAM, which allows for continuous imaging particle analysis. The FlowCAM works by continuously imaging particles as they flow through its flow cell. It can collect thousands of images per minute and measure particles based on various parameters. The document demonstrates the FlowCAM by analyzing two drug formulations, removing unwanted silicon oil droplets and air bubbles, and providing statistics on particle concentrations greater than 10um and 25um. It finds that formulation B contains many more outsized particles than formulation A, so formulation B is more likely to cause complications in patients. The FlowCAM offers the speed of rapid particle analysis technologies with the enhanced ability to identify and qualify different particle
This document discusses social media and new communication platforms. It defines social media and outlines different types of social media users. It also discusses how businesses can use social networking to listen to customers, participate in conversations, and be committed through setting goals and measuring results. The document concludes by discussing emerging platforms like Google Wave and how they may change how people communicate and collaborate online.
The document discusses testing viscosity and particle size of a VLP sample over time using two techniques: laser diffraction and dynamic light scattering. It performed the tests on the initial VLP sample and again after 48 hours, and took 3 replicates of each measurement using the different instruments to analyze which technique provided more consistent and precise results for particle size analysis of this VLP sample over time.
The document discusses testing viscosity and particle size of a VLP sample over time using two techniques: laser diffraction and dynamic light scattering. It performed the tests on the initial VLP sample and again after 48 hours, and took 3 replicates of each measurement using the different instruments to analyze which technique provided more consistent and precise results for particle size analysis of this VLP sample over time.
TrustArc Webinar - 2024 Global Privacy SurveyTrustArc
How does your privacy program stack up against your peers? What challenges are privacy teams tackling and prioritizing in 2024?
In the fifth annual Global Privacy Benchmarks Survey, we asked over 1,800 global privacy professionals and business executives to share their perspectives on the current state of privacy inside and outside of their organizations. This year’s report focused on emerging areas of importance for privacy and compliance professionals, including considerations and implications of Artificial Intelligence (AI) technologies, building brand trust, and different approaches for achieving higher privacy competence scores.
See how organizational priorities and strategic approaches to data security and privacy are evolving around the globe.
This webinar will review:
- The top 10 privacy insights from the fifth annual Global Privacy Benchmarks Survey
- The top challenges for privacy leaders, practitioners, and organizations in 2024
- Key themes to consider in developing and maintaining your privacy program
Skybuffer SAM4U tool for SAP license adoptionTatiana Kojar
Manage and optimize your license adoption and consumption with SAM4U, an SAP free customer software asset management tool.
SAM4U, an SAP complimentary software asset management tool for customers, delivers a detailed and well-structured overview of license inventory and usage with a user-friendly interface. We offer a hosted, cost-effective, and performance-optimized SAM4U setup in the Skybuffer Cloud environment. You retain ownership of the system and data, while we manage the ABAP 7.58 infrastructure, ensuring fixed Total Cost of Ownership (TCO) and exceptional services through the SAP Fiori interface.
HCL Notes and Domino License Cost Reduction in the World of DLAUpanagenda
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-and-domino-license-cost-reduction-in-the-world-of-dlau/
The introduction of DLAU and the CCB & CCX licensing model caused quite a stir in the HCL community. As a Notes and Domino customer, you may have faced challenges with unexpected user counts and license costs. You probably have questions on how this new licensing approach works and how to benefit from it. Most importantly, you likely have budget constraints and want to save money where possible. Don’t worry, we can help with all of this!
We’ll show you how to fix common misconfigurations that cause higher-than-expected user counts, and how to identify accounts which you can deactivate to save money. There are also frequent patterns that can cause unnecessary cost, like using a person document instead of a mail-in for shared mailboxes. We’ll provide examples and solutions for those as well. And naturally we’ll explain the new licensing model.
Join HCL Ambassador Marc Thomas in this webinar with a special guest appearance from Franz Walder. It will give you the tools and know-how to stay on top of what is going on with Domino licensing. You will be able lower your cost through an optimized configuration and keep it low going forward.
These topics will be covered
- Reducing license cost by finding and fixing misconfigurations and superfluous accounts
- How do CCB and CCX licenses really work?
- Understanding the DLAU tool and how to best utilize it
- Tips for common problem areas, like team mailboxes, functional/test users, etc
- Practical examples and best practices to implement right away
Monitoring and Managing Anomaly Detection on OpenShift.pdfTosin Akinosho
Monitoring and Managing Anomaly Detection on OpenShift
Overview
Dive into the world of anomaly detection on edge devices with our comprehensive hands-on tutorial. This SlideShare presentation will guide you through the entire process, from data collection and model training to edge deployment and real-time monitoring. Perfect for those looking to implement robust anomaly detection systems on resource-constrained IoT/edge devices.
Key Topics Covered
1. Introduction to Anomaly Detection
- Understand the fundamentals of anomaly detection and its importance in identifying unusual behavior or failures in systems.
2. Understanding Edge (IoT)
- Learn about edge computing and IoT, and how they enable real-time data processing and decision-making at the source.
3. What is ArgoCD?
- Discover ArgoCD, a declarative, GitOps continuous delivery tool for Kubernetes, and its role in deploying applications on edge devices.
4. Deployment Using ArgoCD for Edge Devices
- Step-by-step guide on deploying anomaly detection models on edge devices using ArgoCD.
5. Introduction to Apache Kafka and S3
- Explore Apache Kafka for real-time data streaming and Amazon S3 for scalable storage solutions.
6. Viewing Kafka Messages in the Data Lake
- Learn how to view and analyze Kafka messages stored in a data lake for better insights.
7. What is Prometheus?
- Get to know Prometheus, an open-source monitoring and alerting toolkit, and its application in monitoring edge devices.
8. Monitoring Application Metrics with Prometheus
- Detailed instructions on setting up Prometheus to monitor the performance and health of your anomaly detection system.
9. What is Camel K?
- Introduction to Camel K, a lightweight integration framework built on Apache Camel, designed for Kubernetes.
10. Configuring Camel K Integrations for Data Pipelines
- Learn how to configure Camel K for seamless data pipeline integrations in your anomaly detection workflow.
11. What is a Jupyter Notebook?
- Overview of Jupyter Notebooks, an open-source web application for creating and sharing documents with live code, equations, visualizations, and narrative text.
12. Jupyter Notebooks with Code Examples
- Hands-on examples and code snippets in Jupyter Notebooks to help you implement and test anomaly detection models.
Dive into the realm of operating systems (OS) with Pravash Chandra Das, a seasoned Digital Forensic Analyst, as your guide. 🚀 This comprehensive presentation illuminates the core concepts, types, and evolution of OS, essential for understanding modern computing landscapes.
Beginning with the foundational definition, Das clarifies the pivotal role of OS as system software orchestrating hardware resources, software applications, and user interactions. Through succinct descriptions, he delineates the diverse types of OS, from single-user, single-task environments like early MS-DOS iterations, to multi-user, multi-tasking systems exemplified by modern Linux distributions.
Crucial components like the kernel and shell are dissected, highlighting their indispensable functions in resource management and user interface interaction. Das elucidates how the kernel acts as the central nervous system, orchestrating process scheduling, memory allocation, and device management. Meanwhile, the shell serves as the gateway for user commands, bridging the gap between human input and machine execution. 💻
The narrative then shifts to a captivating exploration of prominent desktop OSs, Windows, macOS, and Linux. Windows, with its globally ubiquitous presence and user-friendly interface, emerges as a cornerstone in personal computing history. macOS, lauded for its sleek design and seamless integration with Apple's ecosystem, stands as a beacon of stability and creativity. Linux, an open-source marvel, offers unparalleled flexibility and security, revolutionizing the computing landscape. 🖥️
Moving to the realm of mobile devices, Das unravels the dominance of Android and iOS. Android's open-source ethos fosters a vibrant ecosystem of customization and innovation, while iOS boasts a seamless user experience and robust security infrastructure. Meanwhile, discontinued platforms like Symbian and Palm OS evoke nostalgia for their pioneering roles in the smartphone revolution.
The journey concludes with a reflection on the ever-evolving landscape of OS, underscored by the emergence of real-time operating systems (RTOS) and the persistent quest for innovation and efficiency. As technology continues to shape our world, understanding the foundations and evolution of operating systems remains paramount. Join Pravash Chandra Das on this illuminating journey through the heart of computing. 🌟
leewayhertz.com-AI in predictive maintenance Use cases technologies benefits ...alexjohnson7307
Predictive maintenance is a proactive approach that anticipates equipment failures before they happen. At the forefront of this innovative strategy is Artificial Intelligence (AI), which brings unprecedented precision and efficiency. AI in predictive maintenance is transforming industries by reducing downtime, minimizing costs, and enhancing productivity.
Generating privacy-protected synthetic data using Secludy and MilvusZilliz
During this demo, the founders of Secludy will demonstrate how their system utilizes Milvus to store and manipulate embeddings for generating privacy-protected synthetic data. Their approach not only maintains the confidentiality of the original data but also enhances the utility and scalability of LLMs under privacy constraints. Attendees, including machine learning engineers, data scientists, and data managers, will witness first-hand how Secludy's integration with Milvus empowers organizations to harness the power of LLMs securely and efficiently.
Nunit vs XUnit vs MSTest Differences Between These Unit Testing Frameworks.pdfflufftailshop
When it comes to unit testing in the .NET ecosystem, developers have a wide range of options available. Among the most popular choices are NUnit, XUnit, and MSTest. These unit testing frameworks provide essential tools and features to help ensure the quality and reliability of code. However, understanding the differences between these frameworks is crucial for selecting the most suitable one for your projects.
Introduction of Cybersecurity with OSS at Code Europe 2024Hiroshi SHIBATA
I develop the Ruby programming language, RubyGems, and Bundler, which are package managers for Ruby. Today, I will introduce how to enhance the security of your application using open-source software (OSS) examples from Ruby and RubyGems.
The first topic is CVE (Common Vulnerabilities and Exposures). I have published CVEs many times. But what exactly is a CVE? I'll provide a basic understanding of CVEs and explain how to detect and handle vulnerabilities in OSS.
Next, let's discuss package managers. Package managers play a critical role in the OSS ecosystem. I'll explain how to manage library dependencies in your application.
I'll share insights into how the Ruby and RubyGems core team works to keep our ecosystem safe. By the end of this talk, you'll have a better understanding of how to safeguard your code.
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...Jeffrey Haguewood
Sidekick Solutions uses Bonterra Impact Management (fka Social Solutions Apricot) and automation solutions to integrate data for business workflows.
We believe integration and automation are essential to user experience and the promise of efficient work through technology. Automation is the critical ingredient to realizing that full vision. We develop integration products and services for Bonterra Case Management software to support the deployment of automations for a variety of use cases.
This video focuses on integration of Salesforce with Bonterra Impact Management.
Interested in deploying an integration with Salesforce for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
Digital Marketing Trends in 2024 | Guide for Staying AheadWask
https://www.wask.co/ebooks/digital-marketing-trends-in-2024
Feeling lost in the digital marketing whirlwind of 2024? Technology is changing, consumer habits are evolving, and staying ahead of the curve feels like a never-ending pursuit. This e-book is your compass. Dive into actionable insights to handle the complexities of modern marketing. From hyper-personalization to the power of user-generated content, learn how to build long-term relationships with your audience and unlock the secrets to success in the ever-shifting digital landscape.
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAUpanagenda
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-und-domino-lizenzkostenreduzierung-in-der-welt-von-dlau/
DLAU und die Lizenzen nach dem CCB- und CCX-Modell sind für viele in der HCL-Community seit letztem Jahr ein heißes Thema. Als Notes- oder Domino-Kunde haben Sie vielleicht mit unerwartet hohen Benutzerzahlen und Lizenzgebühren zu kämpfen. Sie fragen sich vielleicht, wie diese neue Art der Lizenzierung funktioniert und welchen Nutzen sie Ihnen bringt. Vor allem wollen Sie sicherlich Ihr Budget einhalten und Kosten sparen, wo immer möglich. Das verstehen wir und wir möchten Ihnen dabei helfen!
Wir erklären Ihnen, wie Sie häufige Konfigurationsprobleme lösen können, die dazu führen können, dass mehr Benutzer gezählt werden als nötig, und wie Sie überflüssige oder ungenutzte Konten identifizieren und entfernen können, um Geld zu sparen. Es gibt auch einige Ansätze, die zu unnötigen Ausgaben führen können, z. B. wenn ein Personendokument anstelle eines Mail-Ins für geteilte Mailboxen verwendet wird. Wir zeigen Ihnen solche Fälle und deren Lösungen. Und natürlich erklären wir Ihnen das neue Lizenzmodell.
Nehmen Sie an diesem Webinar teil, bei dem HCL-Ambassador Marc Thomas und Gastredner Franz Walder Ihnen diese neue Welt näherbringen. Es vermittelt Ihnen die Tools und das Know-how, um den Überblick zu bewahren. Sie werden in der Lage sein, Ihre Kosten durch eine optimierte Domino-Konfiguration zu reduzieren und auch in Zukunft gering zu halten.
Diese Themen werden behandelt
- Reduzierung der Lizenzkosten durch Auffinden und Beheben von Fehlkonfigurationen und überflüssigen Konten
- Wie funktionieren CCB- und CCX-Lizenzen wirklich?
- Verstehen des DLAU-Tools und wie man es am besten nutzt
- Tipps für häufige Problembereiche, wie z. B. Team-Postfächer, Funktions-/Testbenutzer usw.
- Praxisbeispiele und Best Practices zum sofortigen Umsetzen
Letter and Document Automation for Bonterra Impact Management (fka Social Sol...Jeffrey Haguewood
Sidekick Solutions uses Bonterra Impact Management (fka Social Solutions Apricot) and automation solutions to integrate data for business workflows.
We believe integration and automation are essential to user experience and the promise of efficient work through technology. Automation is the critical ingredient to realizing that full vision. We develop integration products and services for Bonterra Case Management software to support the deployment of automations for a variety of use cases.
This video focuses on automated letter generation for Bonterra Impact Management using Google Workspace or Microsoft 365.
Interested in deploying letter generation automations for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
Best 20 SEO Techniques To Improve Website Visibility In SERPPixlogix Infotech
Boost your website's visibility with proven SEO techniques! Our latest blog dives into essential strategies to enhance your online presence, increase traffic, and rank higher on search engines. From keyword optimization to quality content creation, learn how to make your site stand out in the crowded digital landscape. Discover actionable tips and expert insights to elevate your SEO game.
Best 20 SEO Techniques To Improve Website Visibility In SERP
Commercial Applications of Stem Cells: NIH Lecture 06 Oct 2010
1. Commercial Applications of Stem Cells NIH Workshop Lecture Bethesda, Md Oct 6th, 2010 By: Jim Hardy President & CSO HemaCell Perfusion, Inc.
2. Background Wittenberg University University of Rochester BRL/Life Technologies, Inc. BP Solar In Vitro Technologies LavaAmp & Hemacell Perfusion @ FITCI
3. Stem Cell Market Projections and Time Line RegMed 2.0 USD (millions) 1961: Canadians Jim Till & Ernest McCulloch prove the existence of stem cells (Nature 1963) 1978: Hematopoietic SC discovered in UCB 1981: mouse ES cells isolated 1988: HSC isolated from blood 1992: Adult neural SC discovered 1994: first Cancer SC discovered 1996: Dolly the Sheep 1998: first human ES line
4. The Gartner “Hype Cycle” http://en.wikipedia.org/wiki/File:Gartner_Hype_Cycle.svg
6. Picks and Shovels Tissue sourcing, collection, isolation and banking Cell Culture medium, Growth factors, cytokines, ECM proteins The challenges of ex vivo expansion QC Testing, characterization Stem cells in Drug Discovery
25. Stem cells in Drug Discovery As a replacement for Primary Cells Skin: Wound healing & absorption models Bone, cartilage Hepatocytes Renal cells Circulatory Cardiomyocytes Neurons Pulmonary cells In vitro models for metabolic homeostasis & Organogenesis Demonstrate a “stimulation” of injury & repair mechanisms Inhibition of necrosis/apoptosis Tissue specific developmental pathways Cancer Stem Cells
26. Alzheimer's Disease and neurodegeneration: The modular systems biology approach to investigate the control of apoptosis in Alzheimer's disease neurodegeneration, BMC Neurosci. 2006; 7(Suppl 1): S2.
29. Conclusions A business can be built around manufacturing almost any of the tools for Stem Cell research which precedes clinical testing. Stem Cell research holds the promise of a new era in medicine, a Gold Rush of sorts. Before any EUREKA moment, researchers are going to need picks and shovels to find the Gold. In other words, before the FDA approves the use of manipulated cells for therapeutic purposes, investigators will need to completely understand the systems they’re working with .
Editor's Notes
One“trigger” event was Dolly the Sheep in ‘96. Another was the “discovery” of iPS cells only a few years ago.
There are still a few clinical applications today. The primary source of transplantable material is Bone Marrow derived HSC. We need to do a lot of research before we strike it rich. We need to tools to find the gold.The business plan is analogous to being a “Hardware store” in a Gold mining town
So we’re going to discuss some of the tools of the trade, the picks and shovels and the commerce around them.I should add some of my personal bias, to be fair. My work and background is with Human Adult Stem Cells, so that is my focus. Specifically, I am focused on using whole afterbirth, not just the Cord Blood and Placental blood, but the whole organ: cord, amnion, and placental tissue as well as the blood components.Keep in mind that there are parallel paths, often more advanced, in non-human and veterinary model systems. There are also a number of areas, ex vivo expansion for example, that are much more detail for ES cells than adult cells.
The “grave diggers” and unethical morticians get a lot of press and attention and the damage these criminals do goes far beyond the corpses they’ve desecrated. It has perpetrated a myth that all stem cell research is illegal. That tissue banking is an unethical practice.There is a huge business in collecting tissues for organ transplantation and in performing transplants. But not all tissue consented for transplant can be matched with a suitable recipient. I like to think that research use fills a void for the grieving family, hoping make some good from their loss.The Organ and Tissue Act set conditions for tissue collection and the necessity of “informed consent.” GTP (Good Tissue Practices) analogous to GMP, regulates the storage and “quality” of tissues used in clinical applications.But any organ/tissue being used to isolate stem cells needs to be fresh, viable and free of contamination.And like I said, I am not an ES practitioner, so I am not raid IVF clinics waiting for cast offs, but I ask: what is wrong with saving these fertilized eggs from the autoclave and using them to save humanity from unnecessary pain and suffering?
Most OPOs and Tissue banks are “Non-Profits”, but this does not mean they’re not generating commerce and revenue and paying a lot of salaries. They serve a necessary a valuable purpose that is really just starting to be realized.
Look at this partial list of Accredited Tissue BanksSurgical resection includes adipose tissue from “tummy tucks” cosmetic surgery, cancerous tumors, normal tissue adjacent to a tumor in large organs like liver, brain and skin.
A typical Cord Blood collection kitDiscuss costs and benefit. Public Cord banking is the way to go. Save the Afterbirth!!DIY Regenerative Medicine, Open ScienceMediStem ‘s regenerative endometrial cells.“Your Monthly Miracle”
Density Gradient sedimentation or centrifugation is the simplest, most broadly used preparative technique. Ficoll, Percoll, Lymphocyte separation medium, Hetastarch. Many examples from at least a dozen different sources.Minimal manipulation to keep within the GTP and not have to deal with more rigorous “GMP” regulations.
Overview of technologyWaters, BD, Coulter flow cytometers costs, reagents, disposables
And everything, virtually, has to be stored. Takes people, and computers and logistics, inventory control. And how much do you think each one of those MVE’s costs? And How much LN2 does it take to keep them full? Note the Controlled rate freezers. This is commerce. Manufacturing.
This is an area I am not going to go into a great level of detail, but these tools, picks and shovels, are perhaps some of the most important and some of the most versatile tools for the Stem Cell researcher. And believe me, you don ‘t want to see a Cell Culture guy out there bleeding goats to make their own antibodies.But look at the complexity of something as simple as DMEM. Still mostly bottled water.
Most involve the use of antibodies and expensive equipment.
chemical signals (polypeptide growth factors and cytokines) in the local tissue environment control growth and specialization of different cell types of the prostate, the liver, the vascular system and neural tissue. These signals determine the normal development and function of the tissues while aberrations result in tissue dysfunction and diseases, such as cancer, stroke, atherosclerosis, liver, and neural disease. These signaling systems which are comprised of a signal polypeptide from one cell type and a reception system on another are the basis for communication among cells in tissues, but also serve as sensors of signals like hormones and nutrients that come from outside the tissues. The cellular reception system for many signal polypeptides consists of a transmembrane protein whose external domain interacts with signal polypeptides and an intracellular domain which is a protein kinase enzyme which activates metabolic pathways that control cell growth, function, and gene expression.
NFT = neurofibrillary tanglestrkA = tyrosine kinaseThus, the phenomenon of apoptosis, that is central to the Neurotrophic Factor Hypothesis, was first observed in the context of the discovery of NGF and its role on differentiation and survival of sympathetic and sensory neurons of the peripheral nervous system [70,71]. During development, neurotrophins regulate neuronal survival and differentiation, and determine the pattern of innervation and the expression of proteins that are crucial to a specific neuronal phenotype, like neurotransmitters and neuropeptides, neurotransmitter receptors and ion channels. According to the neurotrophic hypothesis, the correct pattern of innervation is dependent upon competition of developing neurons for a limited supply of growth factors secreted by target tissues, thus ensuring a balance between the number of neurons and the size of the innervated targetexcitotoxicitydue to overactivation of glutamate receptors represents a final common pathway for both acute neurological disorders, such as stroke, trauma and epileptic seizures, and chronic neurodegenerations like AD