Jim Hardy discusses the commercial applications of stem cells. He outlines the history of stem cell discoveries and projections for the stem cell market. Some commercial opportunities include tissue sourcing, cell culture materials, tools for cell expansion and characterization, and using stem cells in drug discovery as replacements for primary cells in testing and disease modeling. Challenges include scaling ex vivo cell expansion and understanding tissue-specific developmental pathways well enough for therapeutic applications.
WEBINAR Characterisation of human pluripotent stem cells (ESCs and IPSC) and ...Quality Assistance s.a.
Valérie DEFFONTAINE, R&D Scientist, Quality Assistance
Webinar held on 8th June 2017.
The discovery of human pluripotent stem cells 10 years ago turned the spotlight on the potential of pluripotent stem cells for personalised cell therapy. The scientific interest then quickly shifted towards the use of these cells for safety pharmacology, drug discovery and disease modelling. For all these purposes, in the mid to long term, properly characterised cell banks will be necessary.
The characterisation of embryonic (ESC) and induced pluripotent stem cells (IPSC) used for manufacturing requires the development and validation of analytical methods (e.g. flow cytometry, microscopy, QPCR and bioassays). Cell characterisation includes the testing of cell product identity, determination of impurities, and assessment of biological activity and viability. Among the techniques available, flow cytometry is widely used to assess the expression of cell markers. Our laboratory has developed flow cytometry panels dedicated to the characterisation of extracellular and intracellular markers of ESC and IPSC, and to the detection of cell-related impurities. We proposed a method for the validation of flow cytometry panels according to the recommendations of international guidelines on the validation of analytical methods.
IPSC differentiated into cardiomyocytes and MSC-like cells were also used to test the performance of our flow cytometry panels to accurately monitor the manufacturing process of cell products.
In addition to the technical tips, this webinar aims at presenting a critical view on the use of flow cytometry platform for cell characterisation.
For more information, visit http://www.quality-assistance.com/analytical-services/CBMPs
Development of quality control assays for cell-based medicinal products (ISCT...Quality Assistance s.a.
Dr. Fabian Vandermeers from Quality Assistance spoke on Development of quality control assays for cell-based medicinal products at ISCT 2017 in London.
For more information on this topic, visit: http://www.quality-assistance.com/analytical-services/CBMPs
For more information on our expertise and services, visit: www.quality-assistance.com
Follow us on social media:
LinkedIn: https://www.linkedin.com/company/quality-assistance
Twitter: https://twitter.com/QA_Belgium
Facebook: https://www.facebook.com/QualityAssistanceBelgium
Google +: https://plus.google.com/103676189647965359292
Quality Assistance S.A. is a leading European Contract Research Organisation providing the pharmaceutical industry with all the analytical services required by EMA and FDA regulations for the development and marketing of innovative human medicinal products.
We assist our clients from candidate selection, through non-clinical and clinical studies, to marketing authorisation, using our state-of-the-art, product-dedicated expertise in analytical sciences.
For each customer and each project, we design customised solutions, define analytical protocols, develop and validate specific new analytical methods and perform characterisation, stability, pharmacokinetic, biomarker and immunogenicity studies as well as batch release testing, in order to evaluate the Quality, Safety and Efficacy of the given drugs.
Kinases belong to the family of phosphotransferases and play critical roles in the process known as phosphorylation in which a phosphorylated substrate and ADP are produced.
https://www.creative-biogene.com/products/kinase-stable-cell-lines.html
WEBINAR Characterisation of human pluripotent stem cells (ESCs and IPSC) and ...Quality Assistance s.a.
Valérie DEFFONTAINE, R&D Scientist, Quality Assistance
Webinar held on 8th June 2017.
The discovery of human pluripotent stem cells 10 years ago turned the spotlight on the potential of pluripotent stem cells for personalised cell therapy. The scientific interest then quickly shifted towards the use of these cells for safety pharmacology, drug discovery and disease modelling. For all these purposes, in the mid to long term, properly characterised cell banks will be necessary.
The characterisation of embryonic (ESC) and induced pluripotent stem cells (IPSC) used for manufacturing requires the development and validation of analytical methods (e.g. flow cytometry, microscopy, QPCR and bioassays). Cell characterisation includes the testing of cell product identity, determination of impurities, and assessment of biological activity and viability. Among the techniques available, flow cytometry is widely used to assess the expression of cell markers. Our laboratory has developed flow cytometry panels dedicated to the characterisation of extracellular and intracellular markers of ESC and IPSC, and to the detection of cell-related impurities. We proposed a method for the validation of flow cytometry panels according to the recommendations of international guidelines on the validation of analytical methods.
IPSC differentiated into cardiomyocytes and MSC-like cells were also used to test the performance of our flow cytometry panels to accurately monitor the manufacturing process of cell products.
In addition to the technical tips, this webinar aims at presenting a critical view on the use of flow cytometry platform for cell characterisation.
For more information, visit http://www.quality-assistance.com/analytical-services/CBMPs
Development of quality control assays for cell-based medicinal products (ISCT...Quality Assistance s.a.
Dr. Fabian Vandermeers from Quality Assistance spoke on Development of quality control assays for cell-based medicinal products at ISCT 2017 in London.
For more information on this topic, visit: http://www.quality-assistance.com/analytical-services/CBMPs
For more information on our expertise and services, visit: www.quality-assistance.com
Follow us on social media:
LinkedIn: https://www.linkedin.com/company/quality-assistance
Twitter: https://twitter.com/QA_Belgium
Facebook: https://www.facebook.com/QualityAssistanceBelgium
Google +: https://plus.google.com/103676189647965359292
Quality Assistance S.A. is a leading European Contract Research Organisation providing the pharmaceutical industry with all the analytical services required by EMA and FDA regulations for the development and marketing of innovative human medicinal products.
We assist our clients from candidate selection, through non-clinical and clinical studies, to marketing authorisation, using our state-of-the-art, product-dedicated expertise in analytical sciences.
For each customer and each project, we design customised solutions, define analytical protocols, develop and validate specific new analytical methods and perform characterisation, stability, pharmacokinetic, biomarker and immunogenicity studies as well as batch release testing, in order to evaluate the Quality, Safety and Efficacy of the given drugs.
Kinases belong to the family of phosphotransferases and play critical roles in the process known as phosphorylation in which a phosphorylated substrate and ADP are produced.
https://www.creative-biogene.com/products/kinase-stable-cell-lines.html
The discovery that somatic cells could be reprogrammed to a pluripotent state has profoundly altered the landscape in which stem cell research is conducted.
Induced Pluripotent Stem Cells (iPSCs) are a type pf pluripotent stem cell artificially derived, and often referred to as programmed, from adult somatic cells using the expression of certain genes in culture.
https://www.creative-bioarray.com/products/ipsc-reprogramming-kit-list-239.htm
Acroscell provides ready-to-use beating human induced pluripotent stem cells (iPSC)-derived cardiomyocytes. Generated from mature cells that have been genetically reprogramed to a pluripotent stem cell state, induced pluripotent stem cells (iPSCs) can be readily expanded and induced to specialize or differentiate into cardiomyocytes in vitro.
https://www.creative-bioarray.com/acroscell/ipsc-derived-cardiomyocytes.html
Sequencing and functional genomics Unit_Biomedical Research Center of Aragon_...Pilar Mozas
Documento que presenta la cartera de servicios y el equipamiento del Servicio Científico Técnico de Secuenciación y Genómica Funcional de la Universidad de Zaragoza y el Instituto Aragonés de Ciencias de la Salud.
Obesity is a Clinical problem that contributes to life debilitating diseases like diabetes and atherosclerosis.
Adipogenesis in vitro: Cellular differentiation of 3T3-L1 cell line in vitro, treated with dexamethazone, IBMX and insulin leads to accumulation of lipid droplets in adipocytes as (Fig 2.) induced by transcription factors C/EBP-alpha and PPAR-gamma which in turn activate expression of adipocyte specific genes like fatty acid synthase and leptin (as shown by qPCR assays in Fig 3).
Why Use Reference Materials (DNA: Reference standadrds provide a consistent and reliable resource for evaluating and optimizing various stages in your sample processing workflow. Whether you’re starting from DNA extraction, assay design, or library preparation, our reference materials can help you to identify and eliminate sources of variability within your protocols.
@AppliedStemCell offers validated, cellular reference standards for direct incorporation into sample processing workflows or quality control processes.
Highlights:
Overview of molecular reference materials
Workflow and QC for ONCOREF™ cell line generation (#CRISPR)
Advantages of CRISPR-engineered molecular reference standards
Applications of reference materials in assay development
Q & A
#sangersequencing #ngs
Selecting a model system is usually one of the first and most challenging steps in exploring a clinical hypothesis, validating a technology, or understanding a biological process. Although cell lines have served the needs of biomedical research for decades, increasingly, grant and publication reviewers as well as agencies regulating drug development, are requesting researchers to reproduce their results in more representative or “clinically relevant” models. This webinar will introduce human and animal-derived primary cells and explain how they currently serve diverse biomedical clinical and research applications as biologically-relevant, species-specific, and simple to use biological systems.
Slides from Andrew Stauffer's presentation at the "Looking to the Future of Shared Print" session held at the ALA Annual Conference on June 27, 2014 in Las Vegas, NV.
The discovery that somatic cells could be reprogrammed to a pluripotent state has profoundly altered the landscape in which stem cell research is conducted.
Induced Pluripotent Stem Cells (iPSCs) are a type pf pluripotent stem cell artificially derived, and often referred to as programmed, from adult somatic cells using the expression of certain genes in culture.
https://www.creative-bioarray.com/products/ipsc-reprogramming-kit-list-239.htm
Acroscell provides ready-to-use beating human induced pluripotent stem cells (iPSC)-derived cardiomyocytes. Generated from mature cells that have been genetically reprogramed to a pluripotent stem cell state, induced pluripotent stem cells (iPSCs) can be readily expanded and induced to specialize or differentiate into cardiomyocytes in vitro.
https://www.creative-bioarray.com/acroscell/ipsc-derived-cardiomyocytes.html
Sequencing and functional genomics Unit_Biomedical Research Center of Aragon_...Pilar Mozas
Documento que presenta la cartera de servicios y el equipamiento del Servicio Científico Técnico de Secuenciación y Genómica Funcional de la Universidad de Zaragoza y el Instituto Aragonés de Ciencias de la Salud.
Obesity is a Clinical problem that contributes to life debilitating diseases like diabetes and atherosclerosis.
Adipogenesis in vitro: Cellular differentiation of 3T3-L1 cell line in vitro, treated with dexamethazone, IBMX and insulin leads to accumulation of lipid droplets in adipocytes as (Fig 2.) induced by transcription factors C/EBP-alpha and PPAR-gamma which in turn activate expression of adipocyte specific genes like fatty acid synthase and leptin (as shown by qPCR assays in Fig 3).
Why Use Reference Materials (DNA: Reference standadrds provide a consistent and reliable resource for evaluating and optimizing various stages in your sample processing workflow. Whether you’re starting from DNA extraction, assay design, or library preparation, our reference materials can help you to identify and eliminate sources of variability within your protocols.
@AppliedStemCell offers validated, cellular reference standards for direct incorporation into sample processing workflows or quality control processes.
Highlights:
Overview of molecular reference materials
Workflow and QC for ONCOREF™ cell line generation (#CRISPR)
Advantages of CRISPR-engineered molecular reference standards
Applications of reference materials in assay development
Q & A
#sangersequencing #ngs
Selecting a model system is usually one of the first and most challenging steps in exploring a clinical hypothesis, validating a technology, or understanding a biological process. Although cell lines have served the needs of biomedical research for decades, increasingly, grant and publication reviewers as well as agencies regulating drug development, are requesting researchers to reproduce their results in more representative or “clinically relevant” models. This webinar will introduce human and animal-derived primary cells and explain how they currently serve diverse biomedical clinical and research applications as biologically-relevant, species-specific, and simple to use biological systems.
Slides from Andrew Stauffer's presentation at the "Looking to the Future of Shared Print" session held at the ALA Annual Conference on June 27, 2014 in Las Vegas, NV.
Gianluca Vadalà, La bellezza delle cellule staminali (Msc and art)Pramantha Arteteca
Nell’ambito della rassegna culturale di arte, scienza e filosofia “Primavera Americana a Decollatura. Sul filo di ordine a caos” organizzata da Pramantha Arte contemporary art gallery
dal 1 al 17 Maggio prossimi presso l’Hotel Cardel di Decollatura, il 10 Maggio l’associazione AstroPramantha aderisce alla giornata nazionale "Occhi su Saturno" con una video proiezione di immagini dal microcosmo del corpo umano nel lavoro del medico ricercatore Gianluca Vadalà dell’Università Campus Bio-Medico di Roma.
Si ringrazia il dott. Gianluca Vadalà per la gentile concessione.
This presentation gives a brief overview of global stem cell market. It first explains what are stem cells and the various types of stem cells. Then we take a look at R&D in the area of stem cell therapies, also called Regenerative Medicine. It then gives a brief overview of some of the global companies active in regenerative medicine space. It is a very nascent area globally, with very few therapies in the market.
In India, there are a few companies like Stempeutics, Reliance Lifesciences and OCT Research focussing on stem cell therapies. Some of these have made false starts, and made a few wrong bets. OCT, a new company, has a promising approach to wound treatment.
raccolta cellule staminali contenute nel sangue del cordone ombelicale
InScientiaFides la biobanca più sicura perchè accreditata FACTNetcord
non permettere che vengano gettati i campioni di sangue cordonale sono una riserva di salute inestimabile
www.inscientiafides.com
Higher expression of SATB2 in hepatocellular carcinoma of African Americans d...rakeshsrivastava89
Abstract
In the United States, Hepatocellular Carcinoma (HCC) incidence has tripled over the past two decades. The disease has disproportionately affected minority and disadvantaged
populations. The purpose of this study was to examine the expression of SATB2 gene in HCC cells derived from African Americans (AA) and Caucasian Americans (CA) and assess its oncogenic potential by measuring cell viability, spheroid
formation, epithelial‐mesenchymal transition (EMT), stem cell markers and pluripotency maintaining factors in cancer stem cells (CSCs). We compared the expression of SATB2 in human primary hepatocytes, HCC cells derived from AA and CA, and
HCC CSCs. Hepatocellular carcinoma cells derived from AA expressed the higher level of SATB2 than those from CA. By comparison, normal human hepatocytes did
not express SATB2. Higher expression of SATB2 in HCC cells from AA was associated with greater growth rate, cell viability, colony formation and EMT characteristics than those from CA. Knockout of SATB2 in CSCs by Crispr/Cas9 technique significantly inhibited the expression of SATB2 gene, stem cell markers (CD24, CD44 and CD133), pluripotency maintaining factors (c‐Myc, KLF4, SOX2 and OCT4), and EMT
compared with non‐targeting control group. The expression of SATB2 was negatively correlated with miR34a. SATB2 rescued the miR‐34a‐mediated inhibition of CSC's viability. These data suggest that SATB2 is an oncogenic factor, and its higher expression may explain the disparity in HCC outcomes among AA.
Wei Yu1 | Sanjit K. Roy2 | Yiming Ma1 | Thomas A. LaVeist3 | Sharmila Shankar1,2,4 |
Rakesh K. Srivastava1,2,4
International Journal of Stem Cell Research and Transplantation (IJST) is an international, Open Access, peer-reviewed journal, which mainly focuses, on the advancements made in the field of cell biology, specifically in the field of Stem Cells.
International Journal of Stem Cell Research and Transplantation (IJST) is a peer-reviewed journal, and is dedicated to providing information with respect to the latest advancements that are being upgraded in our everyday life with respect to the application of Stem cells.
International Journal of Stem Cell Research and Transplantation (IJST) ISSN:2328-3548, is a free, Open Access, Peer-reviewed, exclusive online journal covering areas of Stem cell research, translational work and Clinical studies in the specialty of Stem Cells and Transplantation including allied specialties relevant to the core subject, which is dedicated in publishing high quality manuscripts.
Molecular control of male fertility for crop hybrid breedingSuresh Antre
Harnessing hybrid vigor or heterosis is a promising approach to tackle the current challenge of sustaining enhanced yield gains of field crops. More than half of the production of major crops such as maize, rice, sorghum, rapeseed, and sunflower comes from hybrid varieties.
Determination and comparison rate of expression markers of osteoblast derived...IJERD Editor
Nowadays high accident rates, fractures leading to permanent bone disorders and the impossibility of bone transplant have made scientists to look for new methods of repairing injured bones. Considering the application of stem cells in bone tissue engineering, there exists the necessity to investigate various culture methods and suitable fields and scaffolds. Thus, we decided to induce adipose-derived stem cells into osteoblast cells in two systems of pellet culture and monolayer and compare osteogenic markers. Methods: Stem cells have been separated via mechanical and enzymatic methods and cultured in monolayer and pellet culture models with osteogenic medium. Then, RNA was separated from differentiated cells, complementary DNA (cDNA) was synthesized and amplified. Polymerase chain reaction (PCR) product was transferred to electrophoresis gel. The intensity of the bands was measured by Image-J software and analyzed by SPSS.
SCT60103 March 2017 Assignment - Group 3Yvonne Chin
Compare the use of Lonza KGM Gold Bullet kit and Rheinwald and Green complete FAD medium in primary human epidermal keratinocytes culture and the applicability of cells cultured by these medium in the construction of reconstituted skin equivalent model
Achieving High Yields in Scalable Xeno Free Culture Formats with Mesenchymal ...Merck Life Sciences
Watch the presentation of this webinar here: https://bit.ly/3ryE5ST
Optimize your mesenchymal stem cell growth. Join our webinar to learn more about our GMP-compliant xeno free media formulation that supports high performance expansions and compatibility with scalable xeno free manufacturing conditions.
Optimizing ex vivo cell expansion processes in preparation for clinical use is a critical step in cell therapy manufacturing. Given the curative and lifesaving impacts these therapies can have on patients, overcoming roadblocks with scalability and supply chain, using high quality raw materials are essential for therapeutic access.
The GMP-compliant Stemline® XF MSC Medium and cocktail promotes expansion of human mesenchymal stromal/stem cells (hMSCs) to high densities while maintaining cell identity and quality. This product was designed for derivation and expansion of MSCs using xeno free conditions in planar and microcarrier-based culture platforms, easing the transfer between research, clinical, and manufacturing scale cultures.
In this webinar, you will:
• Explore the current landscape and future trends of cell culture media for adult mesenchymal stem cells
• Discover ways to derive MSC's from Bone Marrow in Xeno Free conditions from static to microcarrier-based suspension culture platforms.
• Learn how Stemline® XF MSC Media provides robust performance and reduces scalability roadblocks
Presented by: Kathleen Ongena, Ph.D., Head of Customer Applications and Mark Ventresco, Cell Therapy Product Manager
Achieving High Yields in Scalable Xeno Free Culture Formats with Mesenchymal ...MilliporeSigma
Watch the presentation of this webinar here: https://bit.ly/3ryE5ST
Optimize your mesenchymal stem cell growth. Join our webinar to learn more about our GMP-compliant xeno free media formulation that supports high performance expansions and compatibility with scalable xeno free manufacturing conditions.
Optimizing ex vivo cell expansion processes in preparation for clinical use is a critical step in cell therapy manufacturing. Given the curative and lifesaving impacts these therapies can have on patients, overcoming roadblocks with scalability and supply chain, using high quality raw materials are essential for therapeutic access.
The GMP-compliant Stemline® XF MSC Medium and cocktail promotes expansion of human mesenchymal stromal/stem cells (hMSCs) to high densities while maintaining cell identity and quality. This product was designed for derivation and expansion of MSCs using xeno free conditions in planar and microcarrier-based culture platforms, easing the transfer between research, clinical, and manufacturing scale cultures.
In this webinar, you will:
• Explore the current landscape and future trends of cell culture media for adult mesenchymal stem cells
• Discover ways to derive MSC's from Bone Marrow in Xeno Free conditions from static to microcarrier-based suspension culture platforms.
• Learn how Stemline® XF MSC Media provides robust performance and reduces scalability roadblocks
Presented by: Kathleen Ongena, Ph.D., Head of Customer Applications and Mark Ventresco, Cell Therapy Product Manager
Биоплазмоника - биочипы для экспресс-диагностики патологий организма и заболе...Dmitriy Filippov
Диагностические устройства на основе эффекта гигантского комбинационного рассеяния света (SERS диагностика ) для анализа молекулярных маркеров патологий в организме (онкологические заболевания, диабет и т.д.).
Высокоточное, быстрое определение уровней гликированных гемоглобинов (HbA1с) и альбуминов (ГлА) в сыворотке крови для диагностики метаболических нарушений в организме (сахарного диабета, почечной недостаточности, гипотиреоза). Возможно расширение области применения: ранняя диагностика онкологических заболеваний, предотвращение угрозы терроризма, распространения опасных и токсичных химических веществ, наркотических веществ, контроль качества сырья.
SERS biochips for express analysis of molecular markers of different diaseses. Quick and presize measurement of HbA1с and albumins in blood serum for diagnosis of diabetes, renal disease, hypothyrosis and cancer.
The differences between a cow and a monkey are clear. It is easy to tell a moth from a mosquito. So why are there still scientific studies that mix them up? The answer is simple: hundreds of cell lines stored and used by modern laboratories have been wrongly identified. Some pig cells are labelled as coming from a chicken; cell lines advertised as human have been shown to contain material from hamsters, rats, mice and monkeys. Problems have already been found with more than 400 cell lines. (Cited from Nature 520 (2015)).
An increasing number of scientific publications (i.e. Nature journals) are now sistematically asking for cell line authentication at the moment of paper submission. To help researchers to meet this requirement, UAT is starting to offer a new service for human cell line authentication.
Poster HCSPHARMA (OncoLilleDays2022) - Mechanobiological characterization of ...HCS Pharma
Thomas Meynard, PhD student in OncoLille (under the supervision of Vincent Senez and Isabelle Van Seuningen) in collaboration with HCS Pharma too, presented a poster showing that it is possible to include BIOMIMESYS® in a microfluidic chip to co-culture Cancer-Associated fibroblasts and cancerous cells, with the aim to increase the complexity and the relevance of in vitro cancer models.
Similar to NIH Lecture Commercial Uses of Stem Cells (20)
Adv. biopharm. APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMSAkankshaAshtankar
MIP 201T & MPH 202T
ADVANCED BIOPHARMACEUTICS & PHARMACOKINETICS : UNIT 5
APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMS By - AKANKSHA ASHTANKAR
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
Integrating Ayurveda into Parkinson’s Management: A Holistic ApproachAyurveda ForAll
Explore the benefits of combining Ayurveda with conventional Parkinson's treatments. Learn how a holistic approach can manage symptoms, enhance well-being, and balance body energies. Discover the steps to safely integrate Ayurvedic practices into your Parkinson’s care plan, including expert guidance on diet, herbal remedies, and lifestyle modifications.
DISSERTATION on NEW DRUG DISCOVERY AND DEVELOPMENT STAGES OF DRUG DISCOVERYNEHA GUPTA
The process of drug discovery and development is a complex and multi-step endeavor aimed at bringing new pharmaceutical drugs to market. It begins with identifying and validating a biological target, such as a protein, gene, or RNA, that is associated with a disease. This step involves understanding the target's role in the disease and confirming that modulating it can have therapeutic effects. The next stage, hit identification, employs high-throughput screening (HTS) and other methods to find compounds that interact with the target. Computational techniques may also be used to identify potential hits from large compound libraries.
Following hit identification, the hits are optimized to improve their efficacy, selectivity, and pharmacokinetic properties, resulting in lead compounds. These leads undergo further refinement to enhance their potency, reduce toxicity, and improve drug-like characteristics, creating drug candidates suitable for preclinical testing. In the preclinical development phase, drug candidates are tested in vitro (in cell cultures) and in vivo (in animal models) to evaluate their safety, efficacy, pharmacokinetics, and pharmacodynamics. Toxicology studies are conducted to assess potential risks.
Before clinical trials can begin, an Investigational New Drug (IND) application must be submitted to regulatory authorities. This application includes data from preclinical studies and plans for clinical trials. Clinical development involves human trials in three phases: Phase I tests the drug's safety and dosage in a small group of healthy volunteers, Phase II assesses the drug's efficacy and side effects in a larger group of patients with the target disease, and Phase III confirms the drug's efficacy and monitors adverse reactions in a large population, often compared to existing treatments.
After successful clinical trials, a New Drug Application (NDA) is submitted to regulatory authorities for approval, including all data from preclinical and clinical studies, as well as proposed labeling and manufacturing information. Regulatory authorities then review the NDA to ensure the drug is safe, effective, and of high quality, potentially requiring additional studies. Finally, after a drug is approved and marketed, it undergoes post-marketing surveillance, which includes continuous monitoring for long-term safety and effectiveness, pharmacovigilance, and reporting of any adverse effects.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
ABDOMINAL TRAUMA in pediatrics part one.drhasanrajab
Abdominal trauma in pediatrics refers to injuries or damage to the abdominal organs in children. It can occur due to various causes such as falls, motor vehicle accidents, sports-related injuries, and physical abuse. Children are more vulnerable to abdominal trauma due to their unique anatomical and physiological characteristics. Signs and symptoms include abdominal pain, tenderness, distension, vomiting, and signs of shock. Diagnosis involves physical examination, imaging studies, and laboratory tests. Management depends on the severity and may involve conservative treatment or surgical intervention. Prevention is crucial in reducing the incidence of abdominal trauma in children.
SURGICAL ANATOMY OF THE RETROPERITONEUM, ADRENALS, KIDNEYS AND URETERS.pptx
NIH Lecture Commercial Uses of Stem Cells
1. Commercial Applications of Stem Cells NIH Workshop Lecture Bethesda, Md Oct 8th, 2009 By: Jim Hardy President & CSO HemaCell Perfusion, Inc.
2. Background Wittenberg University University of Rochester BRL/Life Technologies, Inc. BP Solar In Vitro Technologies Gahaga & HemaCell Perfusion @ FITCI
3. Stem Cell Market Projections and Time Line RegMed 2.0 USD (millions) 1961: Canadians Jim Till & Ernest McCulloch prove the existence of stem cells (Nature 1963) 1978: Hematopoietic SC discovered in UCB 1981: mouse ES cells isolated 1988: HSC isolated from blood 1992: Adult neural SC discovered 1994: first Cancer SC discovered 1996: Dolly the Sheep 1998: first human ES line from the Stem Cell Summit 2007 Fact Sheet: http://www.stemcellsummit.com/2007/stem-cell-fact-sheet.pdf
4. The Gartner “Hype Cycle” http://en.wikipedia.org/wiki/File:Gartner_Hype_Cycle.svg
6. Picks and Shovels Tissue sourcing, collection, isolation and banking Cell Culture medium, Growth factors, cytokines, ECM proteins The challenges of ex vivo expansion QC Testing, characterization Stem cells in Drug Discovery
27. Stem cells in Drug Discovery As a replacement for Primary Cells Skin: Wound healing & absorption models Bone, cartilage Hepatocytes Renal cells Circulatory Cardiomyocytes Neurons Pulmonary cells In vitro models for metabolic homeostasis & Organogenesis Demonstrate a “stimulation” of injury & repair mechanisms Inhibition of necrosis/apoptosis Tissue specific developmental pathways Cancer Stem Cells
28. Alzheimer's Disease and neurodegeneration: The modular systems biology approach to investigate the control of apoptosis in Alzheimer's disease neurodegeneration, BMC Neurosci. 2006; 7(Suppl 1): S2.
30. If there is time Another video http://biobusiness.tv/videos/201
31. Conclusions A business can be built around manufacturing almost any of the tools for Stem Cell research which precedes clinical testing. Stem Cell research holds the promise of a new era in medicine, a Gold Rush of sorts. Before any EUREKA moment, researchers are going to need picks and shovels to find the Gold. In other words, before the FDA approves the use of manipulated cells for therapeutic purposes, investigators will need to completely understand the systems they’re working with .
Editor's Notes
One“trigger” event was Dolly the Sheep in ‘96. Another was the “discovery” of iPS cells only a few years ago.
There are still a few clinical applications today. The primary source of transplantable material is Bone Marrow derived HSC. We need to do a lot of research before we strike it rich. We need to tools to find the gold.The business plan is analogous to being a “Hardware store” in a Gold mining town
So we’re going to discuss some of the tools of the trade, the picks and shovels and the commerce around them.I should add some of my personal bias, to be fair. My work and background is with Human Adult Stem Cells, so that is my focus. Specifically, I am focused on using whole afterbirth, not just the Cord Blood and Placental blood, but the whole organ: cord, amnion, and placental tissue as well as the blood components.Keep in mind that there are parallel paths, often more advanced, in non-human and veterinary model systems. There are also a number of areas, ex vivo expansion for example, that are much more detail for ES cells than adult cells.
The “grave diggers” and unethical morticians get a lot of press and attention and the damage these criminals do goes far beyond the corpses they’ve desecrated. It has perpetrated a myth that all stem cell research is illegal. That tissue banking is an unethical practice.There is a huge business in collecting tissues for organ transplantation and in performing transplants. But not all tissue consented for transplant can be matched with a suitable recipient. I like to think that research use fills a void for the grieving family, hoping make some good from their loss.The Organ and Tissue Act set conditions for tissue collection and the necessity of “informed consent.” GTP (Good Tissue Practices) analogous to GMP, regulates the storage and “quality” of tissues used in clinical applications.But any organ/tissue being used to isolate stem cells needs to be fresh, viable and free of contamination.And like I said, I am not an ES practitioner, so I am not raid IVF clinics waiting for cast offs, but I ask: what is wrong with saving these fertilized eggs from the autoclave and using them to save humanity from unnecessary pain and suffering?
Most OPOs and Tissue banks are “Non-Profits”, but this does not mean they’re not generating commerce and revenue and paying a lot of salaries. They serve a necessary a valuable purpose that is really just starting to be realized.
Look at this partial list of Accredited Tissue BanksSurgical resection includes adipose tissue from “tummy tucks” cosmetic surgery, cancerous tumors, normal tissue adjacent to a tumor in large organs like liver, brain and skin.
A typical Cord Blood collection kitDiscuss costs and benefit. Public Cord banking is the way to go. Save the Afterbirth!!DIY Regenerative Medicine, Open Science
Density Gradient sedimentation or centrifugation is the simplest, most broadly used preparative technique. Ficoll, Percoll, Lymphocyte separation medium, Hetastarch. Many examples from at least a dozen different sources.Minimal manipulation to keep within the GTP and not have to deal with more rigorous “GMP” regulations.
Overview of technologyWaters, BD, Coulter flow cytometers costs, reagents, disposables
And everything, virtually, has to be stored. Takes people, and computers and logistics, inventory control. And how much do you think each one of those MVE’s costs? And How much LN2 does it take to keep them full? Note the Controlled rate freezers. This is commerce. Manufacturing.
This is an area I am not going to go into a great level of detail, but these tools, picks and shovels, are perhaps some of the most important and some of the most versatile tools for the Stem Cell researcher. And believe me, you don ‘t want to see a Cell Culture guy out there bleeding goats to make their own antibodies.But look at the complexity of something as simple as DMEM. Still mostly bottled water.
Most involve the use of antibodies and expensive equipment.
chemical signals (polypeptide growth factors and cytokines) in the local tissue environment control growth and specialization of different cell types of the prostate, the liver, the vascular system and neural tissue. These signals determine the normal development and function of the tissues while aberrations result in tissue dysfunction and diseases, such as cancer, stroke, atherosclerosis, liver, and neural disease. These signaling systems which are comprised of a signal polypeptide from one cell type and a reception system on another are the basis for communication among cells in tissues, but also serve as sensors of signals like hormones and nutrients that come from outside the tissues. The cellular reception system for many signal polypeptides consists of a transmembrane protein whose external domain interacts with signal polypeptides and an intracellular domain which is a protein kinase enzyme which activates metabolic pathways that control cell growth, function, and gene expression.
NFT = neurofibrillary tanglestrkA = tyrosine kinaseThus, the phenomenon of apoptosis, that is central to the Neurotrophic Factor Hypothesis, was first observed in the context of the discovery of NGF and its role on differentiation and survival of sympathetic and sensory neurons of the peripheral nervous system [70,71]. During development, neurotrophins regulate neuronal survival and differentiation, and determine the pattern of innervation and the expression of proteins that are crucial to a specific neuronal phenotype, like neurotransmitters and neuropeptides, neurotransmitter receptors and ion channels. According to the neurotrophic hypothesis, the correct pattern of innervation is dependent upon competition of developing neurons for a limited supply of growth factors secreted by target tissues, thus ensuring a balance between the number of neurons and the size of the innervated targetexcitotoxicitydue to overactivation of glutamate receptors represents a final common pathway for both acute neurological disorders, such as stroke, trauma and epileptic seizures, and chronic neurodegenerations like AD