This document summarizes a collaboration between ME VAC and Merck to develop an optimal filtration process for the production and purification of a foot-and-mouth disease (FMD) vaccine. Key points include: 1) Merck's Cellvento® BHK-200 serum-free medium was used for growing BHK21 cells and replicating the FMD virus. Millipore Express® filters demonstrated good sterilizing-grade filtration capacity for the cell culture media. 2) For clarification, the Millistak+® HC C0HC depth filter showed high capacity and low turbidity, allowing efficient single-step clarification. 3) Pellicon® 2 cassettes