The document summarizes information about monoclonal antibodies (mAbs), including their definition as identical antibodies generated from a single B cell clone that target a single epitope. It describes the advantages of mAbs such as specificity and applications in treatment. The document outlines the preparation process for mAbs and different types including murine, chimeric, humanized, and fully human. It discusses uses of mAbs in diagnosis, therapy, and protein purification and some challenges and future prospects.
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly by plasma cells that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses.
Monoclonal antibodies are important reagents used in biomedical research, in diagnosis of diseases, and in treatment of such diseases as infections and cancer.
These antibodies are produced by cell lines or clones obtained from animals that have been immunized with the substance that is the subject of study.
Production and applications of monoclonal antibodiesKaayathri Devi
production and applications of monoclonal antibodies, monoclonal antibodies ,applications of monoclonal antibodies, production of monoclonal antibodies,
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly by plasma cells that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses.
Monoclonal antibodies are important reagents used in biomedical research, in diagnosis of diseases, and in treatment of such diseases as infections and cancer.
These antibodies are produced by cell lines or clones obtained from animals that have been immunized with the substance that is the subject of study.
Production and applications of monoclonal antibodiesKaayathri Devi
production and applications of monoclonal antibodies, monoclonal antibodies ,applications of monoclonal antibodies, production of monoclonal antibodies,
Monoclonal Antibody-Preparation & Application - MPH201T.pptxRAHUL PAL
Monoclonal antibodies (mAbs) are proteins produced by a single type of B cell. They are identical to each other and recognize a specific antigen. Antigens are molecules that the body's immune system recognizes as foreign. When an antigen binds to a monoclonal antibody, it triggers a series of reactions that can lead to the destruction of the antigen.
Monoclonal antibodies can be used to treat a variety of diseases, including cancer, autoimmune diseases, and infections. They are also used in research and diagnostics.
PREPARATION OF BACTERIAL VACCINES:
Steps involved in killed bacterial vaccine preparation:
1. Selection of an antigen:
The exact strain or strains to be incorporated for preparation of bacterial vaccine.
Eg. Cholera vaccine: smooth strains of the two serological types Inaba and Ogawa
TABC vaccine: O and H antigens in S. typhi and S. paratyphi microorganisms and these organisms also contains Vi antigen.
Each strain is carefully checked for freedom from variation and absence of contaminating organisms.
VIRAL VACCINES
Since viruses are intracellular parasites they will grow only within other living cells.
Methods of viral vaccine production:
Cultivation of virus using free living animals
Fertile eggs
Tissue cultures
General method of the preparation of Bacterial Infections, Toxoids, Viral vaccine, Antitoxins, Serum-immune blood derivatives and other products relative to immunity.
Monoclonal Antibody-Preparation & Application - MPH201T.pptxRAHUL PAL
Monoclonal antibodies (mAbs) are proteins produced by a single type of B cell. They are identical to each other and recognize a specific antigen. Antigens are molecules that the body's immune system recognizes as foreign. When an antigen binds to a monoclonal antibody, it triggers a series of reactions that can lead to the destruction of the antigen.
Monoclonal antibodies can be used to treat a variety of diseases, including cancer, autoimmune diseases, and infections. They are also used in research and diagnostics.
PREPARATION OF BACTERIAL VACCINES:
Steps involved in killed bacterial vaccine preparation:
1. Selection of an antigen:
The exact strain or strains to be incorporated for preparation of bacterial vaccine.
Eg. Cholera vaccine: smooth strains of the two serological types Inaba and Ogawa
TABC vaccine: O and H antigens in S. typhi and S. paratyphi microorganisms and these organisms also contains Vi antigen.
Each strain is carefully checked for freedom from variation and absence of contaminating organisms.
VIRAL VACCINES
Since viruses are intracellular parasites they will grow only within other living cells.
Methods of viral vaccine production:
Cultivation of virus using free living animals
Fertile eggs
Tissue cultures
General method of the preparation of Bacterial Infections, Toxoids, Viral vaccine, Antitoxins, Serum-immune blood derivatives and other products relative to immunity.
Monoclonal Antibodies and it's applications.pptxAfroj Shaikh
SlideShare Description: Monoclonal Antibodies and Their Applications
In the rapidly advancing field of biotechnology, monoclonal antibodies have emerged as powerful tools with diverse applications. This SlideShare presentation provides a comprehensive overview of monoclonal antibodies and their wide-ranging uses in various fields, including medicine, research, and diagnostics.
The presentation begins by explaining the fundamental concept of monoclonal antibodies, highlighting their unique structure and production process. It delves into the significance of hybridoma technology, which allows for the generation of large quantities of identical antibodies derived from a single parental cell line.
Moving on, the SlideShare explores the applications of monoclonal antibodies in the field of medicine. It elucidates how these antibodies are employed in targeted therapies, such as cancer immunotherapy. The presentation highlights the remarkable specificity of monoclonal antibodies in recognizing and binding to specific targets, thereby enabling precise and tailored treatment approaches. It also discusses the role of monoclonal antibodies in autoimmune diseases, infectious diseases, and organ transplantation.
Furthermore, the presentation sheds light on the use of monoclonal antibodies in research and diagnostics. It explains how these antibodies are utilized as indispensable tools in laboratory research, facilitating the identification and characterization of various biomarkers and molecules. It also showcases their utility in techniques such as enzyme-linked immunosorbent assays (ELISA), flow cytometry, and immunohistochemistry.
The SlideShare emphasizes the impact of monoclonal antibodies on the development of novel therapeutic modalities, including antibody-drug conjugates and bispecific antibodies. It touches upon the challenges and future prospects in the field, highlighting ongoing research efforts and advancements in antibody engineering.
With visually appealing slides, concise and informative content, this SlideShare presentation on monoclonal antibodies provides a valuable resource for scientists, healthcare professionals, students, and anyone interested in understanding the significance and applications of these remarkable biotechnological innovations.
Monoclonal antibodies drug targeting particuler carrier systemRoshan Lal Singh
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Literature Review on Development of Monoclonal Antibodies and Hybridoma Techn...Tuhin Samanta
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Monoclonal immunizer (Mab) is a solitary sort of neutralizer that is coordinated against a particular antigenic determinant (epitope). Eternal monoclonal counter acting agent are found in patients experiencing an infection called different myeloma. In 1975 George Kohler and Cesar Milstein were effectively hybridize counter acting agent delivering B-lymphocytes with myeloma cells in vitro and make a hybridoma. The creation of monoclonal immune response by half and half cells is alluded to as hybridoma innovation.
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The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
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Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
1. Seminar on
MONOCLONAL ANTIBODY
Presented by
AYAN PAL
M.PHARM, 2nd SEM
ROLL NO-15920320008
DEPARTMENT OF PHARMACEUTICS
CALCUTTA INSTITUTE OF PHARMACEUTICAL
TECHNOLOGY AND A.H.S,
ULUBERIA,HOWRAH
2. CONTENTS
Basic concepts and introduction
Definition of mAB
Advantages and disadvantages
Preparation of mAB
Types
Evaluation
Application
Problems and future prospects of
monoclonal antibody therapy
Conclusion
References
3. What are antibodies
An antibody is a protein used by immune system to identify and
neutralize foreign objects like bacteria and viruses. Each antibody
recognizes a specific antigen unique to its target.
The high specificity of antibodies makes them an excellent tool for
detecting and quantifying a broad array of targets, from drugs to serum
proteins to microorganisms.
With in vitro assays, antibodies can be used to precipitate soluble
antigens, agglutinate (clump) cells, opsonize and kill bacteria with the
assistance of complement, and neutralize drugs, toxins, and viruses.
4. Monoclonal Antibody
Monoclonal antibodies are identical immunoglobulins,
generated from a single B-cell clone.
These antibodies recognize unique epitopes, or binding sites,
on a single antigen. Derivation from a single B-cell clones and
subsequent targeting of a single epitope is what differentiates
monoclonal antibodies from polyclonal antibodies.
Polyclonal antibodies are antibodies that are derived from
different cell lines. They differ in amino acid sequences.
5. Advantages of using Monoclonal Antibodies
Though expensive, monoclonal antibodies are cheaper to develop than
conventional drugs because it is based on tested technology.
Side effects can be treated and reduced by using mice-human hybrid cells
or by using fractions of antibodies.
They bind to specific diseased or damaged cells needing treatment.
They treat a wide range of conditions.
6. Disadvantages of using Monoclonal Antibodies
Time consuming project - between 6 -9 months.
Very expensive and needs considerable effort to produce them.
Small peptide and fragment antigens may not be good antigens-
monoclonal antibody may not recognize the original antigen.
Hybridoma culture may be subject to contamination.
More than 99% of the cells do not survive during the fusion process –
reducing the range of useful antibodies that can be produced against an
antigen.
10. Murine MAbs
The use of murine antibodies produced by hybridoma technology in human
therapy (clinical medicine) is limited, differences between the human and
rodent immune systems.
Murine antibodies have mild effects of stimulation of cytotoxicity.
Murine mAbs contain foreign protein molecules, majority of the early
reagents for clinical use stimulated unwanted immune responses in human
patients.
The total immunogenicity of the mAb is therefore reduced without affecting
the recognition ability of the original antibody.
11. Chimeric MAbs
Chimeric antibodies are special types of therapeutic antibodies made by the
combination of genetic ingredients from humans and non-humans (mice).
They are produced through manipulation of human constant regions and
mouse variable regions.
chimeric antibodies for use in human therapy and research.
e.g: Infliximab, Rituximab.
12. Humanised MAbs
Human mAbs (HMA) have been considered natural drugs due to their
safety for in vivo activities.
A number of about 20 mAb drugs, including humanized mice mAbs, have
been accepted as therapeutic reagents during the past few decades.
In humanised antibodies, the hyper variable regions are grafted onto
human variable domain framework.
They are sometimes weaker than the parent murine monoclonal
antibodies in terms of binding with antigens.
Eg:- daclizumab, omalizumab.
13. Fully human MAbs
Human mAb production by the conventional hybridoma techniques is
relatively difficult because of the stress involved in maintaining immortalised
cell lines and human hybridomas.
Methods for the production of human mAbs are made possible through the
expression of antibody fragments or single cell variable fragment (Fab or
ScFv) in bacteria.
The phage display technique is a well established and the most widely used
method for the development of new human antibodies.
using transgenic mice containing human immunoglobulins may be a strategy
for the production of human mAbs.
Eg:- Humira®, Adalimumab®, Panitimumab®.
14. Evaluation of Monoclonal Antibody
1. Characterisation of monoclonal antibodies
Physicochemical characterisation
Immunological properties
Biological activity
Purity, impurity and contaminants
Quantity
2. Specifications
Identity
Purity and impurities
Potency
Quantity
General tests
15. Applications of Monoclonal Antobody
A. Diagnostic Applications
1. Biochemical analysis
2. Diagnostic Imaging
B. Therapeutic Applications
1. Direct use of MAbs as therapeutic agents
2. MAbs as targeting agents.
C. Protein Purification
16. 1a. Biochemical analysis
Routinely used in radioimmunoassay (RIA) and enzyme-linked immunosorbent assays
(ELISA) in the laboratory.
These assays measure the circulating concentrations of hormones (insulin, human
chorionic gonadotropin, growth hormone, progesterone, thyroxine, triiodothyronine,
thyroid stimulating hormone) and several other tissue and cell products (blood group
antigens, blood clotting factors, interferon’s, interleukins, tumor markers).
Eg. Pregnancy by detecting the urinary levels of human chorionic gonadotropin.
Hormonal disorders analysis of thyroxine, triiodothyronine. Cancers estimation of
plasma carcinoembryonic antigen in colorectal cancer, and prostate specific antigen for
prostate cancer.
1b. Diagnostic imaging
Radiolabeled—MAbs are used in the diagnostic imaging of diseases, and this technique
is referred to as immunoscintigraphy. The radioisotopes commonly used for labeling
MAb are iodine—131 and technetium—99. The MAb tagged with radioisotope are
injected intravenously into the patients.
These MAbs localize at specific sites (say a tumor) which can be detected by imaging
the radioactivity. In recent years, single photon emission computed tomography
(SPECT) cameras are used to give a more sensitive three dimensional appearance of
the spots localized by radiolabeled— MAbs.
Myocardial infarction, DVT, atherosclorosis etc.
17. 2a. Direct use of MAbs as therapeutic agents
In destroying disease-causing organisms: MAbs promote efficient
opsonization of pathogenic organisms (by coating with antibody) and
enhance phagocytosis.
In the immunosuppression of organ transplantation: In the normal medical
practice, immunosuppressive drugs such as cyclosporin and prednisone are
administered to overcome the rejection of organ transplantation. In recent
years, MAbs specific to T-lymphocyte surface antigens are being used for this
purpose.
19. In the treatment of AIDS
Genetic engineers have been successful
to attach Fc portion of mouse
monoclonal antibody to human CD4
molecule. This complex has high affinity
to bind to membrane glycoprotein gp120
of virus infected cells. The Fc fragment
induces cell-mediated destruction of HIV
infected cells.
20. 2a. MAbs as targeting agents
The drugs can be coupled with MAb (directed against a cell surface
antigen of the cells, say a tumor) and specifically targeted to reach
the site of action.
Eg. Alkaline phosphatase for the conversion of phosphate pro-drugs.
Carboxy peptidase for converting inactive carboxyl pro-drugs to
active drugs.
MAbs in the dissolution of blood clots:
Fibrin is the major constituent of blood clot which gets dissolved by
plasmin. Plasmin in turn is formed by the activation of plasminogen
by plasminogen activator.
21. 3. Protein Purification
Monoclonal antibodies can be produced for any protein. And produced
MAb can be conveniently used for the purification of the protein
against which it was raised.
MAbs columns can be prepared by coupling them to cyanogen
bromide activated Sepharose (chromatographic matrix). The
immobilized MAbs in this manner are very useful for the purification of
proteins by immunoaffinity method.
22. Problems and future prospects of
monoclonal antibody therapy
A mild allergic reaction (rash) may appear with the first administration
of mAbs.
A mAb used against tumor blood vessel growth (Bevacizumab®) can
present numerous side effects, some of which include renal failure,
bleeding with poor wound healing and high blood pressure.
Monoclonal antibodies can also be further modified in order to have
improved effects, through the possibilities of conjugated antibodies
with coupling effector molecules.
23. CONCLUSION
The first mAbs approved as human therapeutic agent were generally
reported to be intolerable as therapeutics, advances in hybridoma
technology.
Multiple engineering efforts have led to the evolution of modified
therapeutic antibodies in the hope of improving their efficacy and
safety as antibody based drugs.
therapeutic mAbs will be commercially more abundant and affordable
in the near future, is in part, a matter of rapid advances in
biotechnology and bio molecular sciences as well as the outcomes of
extensive clinical trials.
24. References
Mahmuda A. , Bande F., Al-Zihiry K. Abdulhaleem N., Majid R., Rukman R.,
Abdullah W. and Unyah Z.(2016). “Monoclonal antibodies: A review of
therapeutic applications and future prospects” Tropical Journal of
Pharmaceutical Research March 2017; 16 (3).
Lu R. Hwang Y.,, Liu J. , Lee C., Tsai H., Li H. and Wu H.
“Development of therapeutic antibodies for the treatment of diseases ”
Journa lof biomedical science, (2020) 27:1
Shivanand P. (2010). “Hybridoma technology for production of monoclonal
antibodies” International Journal of Pharmaceutical Sciences Review and
Research vol.1, issue 2 (017)
U. Marx et al. (1997)“Monoclonal Antibody Production” The Report and
Recommendations of ECVAMWorkshop ATLA 25, 121.137.
Edward A. Greenfield, (2014) “Antibodies: A Laboratory Manual” Cold Spring
Harbor Laboratory Press, 2nd ed. Chapter 7