This document discusses fungal diseases (mycoses) including cutaneous, subcutaneous, and systemic mycoses. It provides examples of common fungal infections like ringworm, athlete's foot, and candidiasis. Systemic mycoses are caused by dimorphic fungi that exist in different forms inside and outside the host. Examples provided are histoplasmosis and coccidiomycosis. Opportunistic fungi like Aspergillus and Candida normally do not cause disease but can in immunocompromised individuals. The document also discusses the biology and pathogenesis of the opportunistic fungus Candida albicans.
This document discusses interferons (IFNs), which are signaling proteins produced by immune cells in response to challenges like viruses and tumors. It describes the three main types of IFNs (Type I, II, and III), their functions in activating the immune system and inhibiting viral replication, and the JAK-STAT signaling pathway involved in their mechanism of action. The document also outlines some applications of IFNs in treating conditions like hepatitis, cancer, and multiple sclerosis as well as potential adverse effects.
The document discusses adaptive immunity and the immune response. It describes the four phases of the adaptive immune response as encounter, activation, attack, and memory. Acquired immunity develops over one's lifetime from vaccines, infections, or antibodies from others. The adaptive immune response involves B cells, helper T cells and cytotoxic T cells. It also discusses the specificity, adaptiveness, discrimination between self and non-self, and memory properties of the adaptive immune response. The major cell types involved are T helper cells, cytotoxic T cells, T memory cells, and regulatory T cells. The roles and mechanisms of these cell types are described in detail.
Biotechnological techniques have been used to produce a number of commercially available products. Despite the ethical issues concerned with this technology, a number of commercial products have been produced.
Competent cell preperation and bacterial transformation.pptAnkitaM23
Component cell preparation and bacterial transformation. ppt
Biotechnology ( plant genetic transformation) second year fourth semester of agricultural biotechnology or agricultural engineering.
Biological contamination is the dread of every person working with cell culture. When cultures become infected with microorganisms, or cross-contaminated by foreign cells, these cultures usually must be destroyed. Since the sources of culture contamination are ubiquitous as well as difficult to identify and eliminate, no cell culture laboratory remains unaffected by this concern. With the continuing increase in the use of cell culture for biological research, vaccine production, and production of therapeutic proteins for personalized medicine and emerging regenerative medicine applications, culture contamination remains a highly important issue. Cell line cross-contamination can be a problem for scientists working with cultured cells. Studies suggest anywhere from 15–20% of the time, cells used in experiments have been misidentified or contaminated with another cell line. Problems with cell line cross-contamination have even been detected in lines from the NCI-60 panel, which are used routinely for drug-screening studies. Major cell line repositories, including the American Type Culture Collection (ATCC), the European Collection of Cell Cultures (ECACC) and the German Collection of Microorganisms and Cell Cultures (DSMZ), have received cell line submissions from researchers that were misidentified by them. Such contamination poses a problem for the quality of research produced using cell culture lines, and the major repositories are now authenticating all cell line submissions. ATCC uses short tandem repeat (STR) DNA fingerprinting to authenticate its cell lines.
Gene therapy is a new tool used in combating different diseases. The majority of gene therapy clinical trials are focused on cancer and so it was no coincidence that the first commercial treatment in 2003 was for neoplasia. Currently there are a wide variety of gene therapy proposals involving a large number of anti tumour molecular mechanisms that will conceivably pave the way for highly effective a treatment options. Despite the significant advances that how been made in gene therapy in the fight against cancer, its efficacy,safety and commercial availability are still limited. Ms. Chetana D. Patil | Ms. Siddhi Chavan | Mr. Ritesh Kadam "Gene Therapy for Cancer Treatment" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26537.pdfPaper URL: https://www.ijtsrd.com/pharmacy/biotechnology-/26537/gene-therapy-for-cancer-treatment/ms-chetana-d-patil
Gene therapy involves inserting normal genes into individuals to replace defective genes that cause disease. It has been used to treat various genetic diseases and cancers since the 1990s. While it offers promise for permanent treatment, gene therapy still faces challenges like short-term effects, immune responses, high costs, and difficulties with gene delivery methods that have limited its effectiveness so far. Continued research aims to overcome these obstacles.
Killed bacterial vaccines against cholera, plague, typhus, and Q fever contain whole killed bacteria and are used to protect those exposed. Live attenuated bacterial vaccines like BCG against tuberculosis use live but weakened Mycobacterium bovis. Toxoid vaccines for diphtheria and tetanus contain inactivated toxins that have lost disease-causing ability but retained immunogenicity. Purified protein vaccines such as the acellular pertussis vaccine recommended for all children contain purified bacterial proteins. Capsular polysaccharide vaccines for pneumonia, meningitis, and H. influenzae conjugate the polysaccharide to a carrier protein to enhance the antibody response.
This document discusses interferons (IFNs), which are signaling proteins produced by immune cells in response to challenges like viruses and tumors. It describes the three main types of IFNs (Type I, II, and III), their functions in activating the immune system and inhibiting viral replication, and the JAK-STAT signaling pathway involved in their mechanism of action. The document also outlines some applications of IFNs in treating conditions like hepatitis, cancer, and multiple sclerosis as well as potential adverse effects.
The document discusses adaptive immunity and the immune response. It describes the four phases of the adaptive immune response as encounter, activation, attack, and memory. Acquired immunity develops over one's lifetime from vaccines, infections, or antibodies from others. The adaptive immune response involves B cells, helper T cells and cytotoxic T cells. It also discusses the specificity, adaptiveness, discrimination between self and non-self, and memory properties of the adaptive immune response. The major cell types involved are T helper cells, cytotoxic T cells, T memory cells, and regulatory T cells. The roles and mechanisms of these cell types are described in detail.
Biotechnological techniques have been used to produce a number of commercially available products. Despite the ethical issues concerned with this technology, a number of commercial products have been produced.
Competent cell preperation and bacterial transformation.pptAnkitaM23
Component cell preparation and bacterial transformation. ppt
Biotechnology ( plant genetic transformation) second year fourth semester of agricultural biotechnology or agricultural engineering.
Biological contamination is the dread of every person working with cell culture. When cultures become infected with microorganisms, or cross-contaminated by foreign cells, these cultures usually must be destroyed. Since the sources of culture contamination are ubiquitous as well as difficult to identify and eliminate, no cell culture laboratory remains unaffected by this concern. With the continuing increase in the use of cell culture for biological research, vaccine production, and production of therapeutic proteins for personalized medicine and emerging regenerative medicine applications, culture contamination remains a highly important issue. Cell line cross-contamination can be a problem for scientists working with cultured cells. Studies suggest anywhere from 15–20% of the time, cells used in experiments have been misidentified or contaminated with another cell line. Problems with cell line cross-contamination have even been detected in lines from the NCI-60 panel, which are used routinely for drug-screening studies. Major cell line repositories, including the American Type Culture Collection (ATCC), the European Collection of Cell Cultures (ECACC) and the German Collection of Microorganisms and Cell Cultures (DSMZ), have received cell line submissions from researchers that were misidentified by them. Such contamination poses a problem for the quality of research produced using cell culture lines, and the major repositories are now authenticating all cell line submissions. ATCC uses short tandem repeat (STR) DNA fingerprinting to authenticate its cell lines.
Gene therapy is a new tool used in combating different diseases. The majority of gene therapy clinical trials are focused on cancer and so it was no coincidence that the first commercial treatment in 2003 was for neoplasia. Currently there are a wide variety of gene therapy proposals involving a large number of anti tumour molecular mechanisms that will conceivably pave the way for highly effective a treatment options. Despite the significant advances that how been made in gene therapy in the fight against cancer, its efficacy,safety and commercial availability are still limited. Ms. Chetana D. Patil | Ms. Siddhi Chavan | Mr. Ritesh Kadam "Gene Therapy for Cancer Treatment" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26537.pdfPaper URL: https://www.ijtsrd.com/pharmacy/biotechnology-/26537/gene-therapy-for-cancer-treatment/ms-chetana-d-patil
Gene therapy involves inserting normal genes into individuals to replace defective genes that cause disease. It has been used to treat various genetic diseases and cancers since the 1990s. While it offers promise for permanent treatment, gene therapy still faces challenges like short-term effects, immune responses, high costs, and difficulties with gene delivery methods that have limited its effectiveness so far. Continued research aims to overcome these obstacles.
Killed bacterial vaccines against cholera, plague, typhus, and Q fever contain whole killed bacteria and are used to protect those exposed. Live attenuated bacterial vaccines like BCG against tuberculosis use live but weakened Mycobacterium bovis. Toxoid vaccines for diphtheria and tetanus contain inactivated toxins that have lost disease-causing ability but retained immunogenicity. Purified protein vaccines such as the acellular pertussis vaccine recommended for all children contain purified bacterial proteins. Capsular polysaccharide vaccines for pneumonia, meningitis, and H. influenzae conjugate the polysaccharide to a carrier protein to enhance the antibody response.
The major histocompatibility complex (MHC) is a collection of genes on chromosome 6 in humans that code for MHC molecules. MHC molecules are divided into three classes: class I molecules are found on almost all nucleated cells and present intracellular peptides; class II molecules are found on antigen-presenting cells and present extracellular peptides; class III molecules are secreted proteins with immune functions like complement components. MHC molecules present peptide fragments to T cells to trigger immune responses and also determine compatibility for organ transplants.
The document discusses various applications of genetic engineering in medicine. It describes how genetic engineering is used to produce insulin, vaccines, monoclonal antibodies, and other drugs through the manipulation of genes and transfer of genes between organisms. The document also explores the future potential of gene therapy and tissue engineering to treat genetic diseases and regenerate tissues.
Cytokines are cell signaling molecules that allow communication between cells of the immune system. They can be classified into six families based on structural similarities and functions. The interleukin (IL) family includes pro-inflammatory cytokines like IL-1 and IL-2 that are secreted by macrophages and T cells respectively to induce immune responses. Chemokines are a subgroup of cytokines that mobilize immune cells between tissues by influencing cytoskeletal proteins and adhesion molecules. Cytokines can have pleiotropic, redundant, synergistic or antagonistic effects on different target cells. Their localized release and cell surface receptor binding allows for precise communication between immune cells.
The document discusses the problem of antibiotic resistance and strategies to contain it. It provides background on antibiotic resistance, describing how it can occur intrinsically in bacteria or be acquired through mutations, plasmids, or gene transfer. Various mechanisms of resistance are outlined, including decreased permeability, efflux pumps, target modification, and antibiotic inactivation. The Indian scenario highlights specific resistance issues. NDM-1 carbapenemase is described as a major concern due to its ability to spread. Containment strategies include developing new antibiotics, judicious antibiotic use, and infection control.
Virology is the study of viruses and virus-like agents, including their taxonomy, disease-producing properties, culture, and genetics. Viruses are non-cellular biological entities that consist of DNA or RNA genomes enclosed in a protein coat. They can only reproduce within living cells. Influenza is caused by RNA viruses of the Orthomyxoviridae family. There are three main types of influenza viruses - A, B, and C. Influenza A is further classified into subtypes based on surface proteins and can infect both humans and animals. Influenza causes respiratory illness with symptoms like fever, cough and sore throat. It spreads through respiratory droplets and the best prevention is an annual flu vaccine.
PHARMACEUTICAL BIOTECHNOLOGY BY PHARM.ISA HASSAN ABUBAKARISAHASSANABUBAKAR68
PHARMACEUTICALS BIOTECHNOLOGY IS A BRANCH OF SCIENCE THAT INVOLVES THE USE OF RECOMBINANT DNA FOR THE EFFECTIVE MANUFACTURE OF SOME EFFECTIVE DRUGS OR MEDICINE,EXAMPLE LIKE RECOMBINANT DNA VACCINE,RECOMBINANT DNA DRUGS,RECOMBINANT DNA ENZYMES,RECOMBINANT DNA INSULIN,RECOMBINANT DNA YEAST E.T.C. NOWADAYS PHARMACEUTICAL INDUSTRIES USES THIS RECOMBINANT DNA IN THE PRODUCTION OF VARIOUS CATEGORIES OF MEDICINES.
PRESENTED BY ISA HASSAN ABUBAKAR FROM NIGERIA
Antibodies have emerged as a promising class of therapeutic drugs due to their high specificity and ability to activate the immune system. Major breakthroughs included the development of serum therapy in the early 1900s and the discovery of monoclonal antibodies in the 1970s-80s. Today, over 20 antibody drugs are approved to treat various diseases including rheumatoid arthritis, with hundreds more in development. Rheumatoid arthritis is an autoimmune disease characterized by joint inflammation and damage, and antibody drugs that target cytokines like TNF-α have proven effective in slowing its progression.
DNA vaccines (types, method and mechanism) Aneela Rafiq
DNA Vaccine is very promising method in current century. it can eliminate the risks of encountering pathogen with living cell.
this presentation has a brief concept about DNA Vaccine, to understand the baseline of genetic vaccine.
This document discusses virulence and the mechanisms by which microorganisms cause disease. It defines virulence as the ability of a microbe to cause disease in a host. Microbes use various virulence factors and mechanisms to evade host defenses, enter and lyse host cells, including adhesion, colonization, invasion, inhibiting the immune response, and producing toxins. These virulence factors are often proteins encoded by genes that can be transferred between microbes. Targeting specific virulence factors and genes has been proposed as a strategy to combat bacterial infections.
Vaccines, types of vaccines, Classification of vaccines, subunit vaccines, attenuated vaccines, live vaccines, inactivated vaccines, recombinant vaccines, DNA vaccines, development of vaccines, future of vaccines, advantages of vaccines, limitation of vaccines, benefits of vaccines.
This document provides information on the laboratory diagnosis of COVID-19. It discusses the types of specimens that can be collected for testing, including nasopharyngeal swabs, sputum, and blood. The main tests for diagnosis are reverse transcriptase PCR tests, which detect viral RNA in specimens. Serological tests that detect antibodies are also used but are not recommended for diagnosis. Factors that can lead to false negative PCR tests are also reviewed, such as inadequate specimen collection or testing outside the diagnostic window. Hematological and biochemical parameters that may be seen in COVID-19 patients are summarized.
This document provides an overview of vaccines including:
- A brief history of vaccines from Edward Jenner's smallpox vaccine to Louis Pasteur's rabies vaccine.
- Descriptions of different types of vaccines including whole organism, subunit, DNA, recombinant vector, and edible vaccines.
- Discussions of immunization programs, challenges in developing an HIV vaccine, the debated link between the MMR vaccine and autism, and the goal of developing more effective and accessible vaccines worldwide.
The document summarizes the replication process of DNA and viruses. It describes how DNA replicates semi-conservatively to produce two identical copies. Viruses like bacteriophage T4 and plant virus TMV are then discussed, with T4 exhibiting a lytic cycle where it hijacks the host cell to produce new virus particles that ultimately burst the cell, while TMV has an RNA genome and protein coat. The stages of viral replication including attachment, penetration, replication of the genome, and assembly of new virus particles are also outlined.
The document discusses anti-viral chemotherapy and considerations for developing effective anti-viral drugs. It notes that ideal drugs are selectively toxic to virus-infected cells, inhibit specific viral enzymes or functions, and have a high therapeutic index. Several classes of anti-viral drugs are described including nucleoside analogs that target viral polymerases, protease inhibitors for HIV, and neuraminidase inhibitors for influenza. Developing drugs that can interfere with viral replication without harming host cells remains an ongoing challenge.
INTRODUCTION OF GENE THERAPY, HISTORY OF GENE THERAPY, Process of gene therapy, Methods of gene therapy, Ex vivo gene therapy , In Vivo Gene Therapy , Uses of gene therapy, Target sites for Gene Therapy , Vectors for gene therapy , Viral Vectors, Non Viral Vectors,
The document outlines the general methods used for vaccine production, which includes 5 main steps: 1) generating the antigen by growing pathogens, 2) releasing and isolating the antigen from growth materials, 3) purifying the antigen using various separation techniques, 4) adding other components like adjuvants and preservatives, 5) packaging the vaccine for distribution while protecting it and the environment. Vaccines work by improving immunity but may not guarantee full protection against disease.
Fungi are eukaryotic organisms that can be single-celled or multicellular. Their cell walls contain chitin and glucans. Fungi can cause superficial infections of the skin and nails, subcutaneous infections beneath the skin, and systemic infections of internal organs. Common fungal diseases include ringworm, athlete's foot, histoplasmosis, and coccidiomycosis. Fungi are classified based on their modes of reproduction and microscopic structures. Identification and diagnosis involves microscopic examination, culturing, and serological tests.
Systemic mycoses can result from inhalation of fungal spores that then differentiate into yeast or other forms in the lungs. This document focuses on four specific systemic mycoses: Coccidioides, Histoplasma, Blastomyces, and Paracoccidioides. Coccidioides causes valley fever through inhalation of spores in dry soil in the southwestern US and Central/South America. Paracoccidioides causes a similar disease through inhalation in parts of Central/South America. Both fungi exist as molds in soil and yeasts in tissues. Symptoms range from asymptomatic to disseminated disease. Diagnosis involves microscopy, culture, and ser
The major histocompatibility complex (MHC) is a collection of genes on chromosome 6 in humans that code for MHC molecules. MHC molecules are divided into three classes: class I molecules are found on almost all nucleated cells and present intracellular peptides; class II molecules are found on antigen-presenting cells and present extracellular peptides; class III molecules are secreted proteins with immune functions like complement components. MHC molecules present peptide fragments to T cells to trigger immune responses and also determine compatibility for organ transplants.
The document discusses various applications of genetic engineering in medicine. It describes how genetic engineering is used to produce insulin, vaccines, monoclonal antibodies, and other drugs through the manipulation of genes and transfer of genes between organisms. The document also explores the future potential of gene therapy and tissue engineering to treat genetic diseases and regenerate tissues.
Cytokines are cell signaling molecules that allow communication between cells of the immune system. They can be classified into six families based on structural similarities and functions. The interleukin (IL) family includes pro-inflammatory cytokines like IL-1 and IL-2 that are secreted by macrophages and T cells respectively to induce immune responses. Chemokines are a subgroup of cytokines that mobilize immune cells between tissues by influencing cytoskeletal proteins and adhesion molecules. Cytokines can have pleiotropic, redundant, synergistic or antagonistic effects on different target cells. Their localized release and cell surface receptor binding allows for precise communication between immune cells.
The document discusses the problem of antibiotic resistance and strategies to contain it. It provides background on antibiotic resistance, describing how it can occur intrinsically in bacteria or be acquired through mutations, plasmids, or gene transfer. Various mechanisms of resistance are outlined, including decreased permeability, efflux pumps, target modification, and antibiotic inactivation. The Indian scenario highlights specific resistance issues. NDM-1 carbapenemase is described as a major concern due to its ability to spread. Containment strategies include developing new antibiotics, judicious antibiotic use, and infection control.
Virology is the study of viruses and virus-like agents, including their taxonomy, disease-producing properties, culture, and genetics. Viruses are non-cellular biological entities that consist of DNA or RNA genomes enclosed in a protein coat. They can only reproduce within living cells. Influenza is caused by RNA viruses of the Orthomyxoviridae family. There are three main types of influenza viruses - A, B, and C. Influenza A is further classified into subtypes based on surface proteins and can infect both humans and animals. Influenza causes respiratory illness with symptoms like fever, cough and sore throat. It spreads through respiratory droplets and the best prevention is an annual flu vaccine.
PHARMACEUTICAL BIOTECHNOLOGY BY PHARM.ISA HASSAN ABUBAKARISAHASSANABUBAKAR68
PHARMACEUTICALS BIOTECHNOLOGY IS A BRANCH OF SCIENCE THAT INVOLVES THE USE OF RECOMBINANT DNA FOR THE EFFECTIVE MANUFACTURE OF SOME EFFECTIVE DRUGS OR MEDICINE,EXAMPLE LIKE RECOMBINANT DNA VACCINE,RECOMBINANT DNA DRUGS,RECOMBINANT DNA ENZYMES,RECOMBINANT DNA INSULIN,RECOMBINANT DNA YEAST E.T.C. NOWADAYS PHARMACEUTICAL INDUSTRIES USES THIS RECOMBINANT DNA IN THE PRODUCTION OF VARIOUS CATEGORIES OF MEDICINES.
PRESENTED BY ISA HASSAN ABUBAKAR FROM NIGERIA
Antibodies have emerged as a promising class of therapeutic drugs due to their high specificity and ability to activate the immune system. Major breakthroughs included the development of serum therapy in the early 1900s and the discovery of monoclonal antibodies in the 1970s-80s. Today, over 20 antibody drugs are approved to treat various diseases including rheumatoid arthritis, with hundreds more in development. Rheumatoid arthritis is an autoimmune disease characterized by joint inflammation and damage, and antibody drugs that target cytokines like TNF-α have proven effective in slowing its progression.
DNA vaccines (types, method and mechanism) Aneela Rafiq
DNA Vaccine is very promising method in current century. it can eliminate the risks of encountering pathogen with living cell.
this presentation has a brief concept about DNA Vaccine, to understand the baseline of genetic vaccine.
This document discusses virulence and the mechanisms by which microorganisms cause disease. It defines virulence as the ability of a microbe to cause disease in a host. Microbes use various virulence factors and mechanisms to evade host defenses, enter and lyse host cells, including adhesion, colonization, invasion, inhibiting the immune response, and producing toxins. These virulence factors are often proteins encoded by genes that can be transferred between microbes. Targeting specific virulence factors and genes has been proposed as a strategy to combat bacterial infections.
Vaccines, types of vaccines, Classification of vaccines, subunit vaccines, attenuated vaccines, live vaccines, inactivated vaccines, recombinant vaccines, DNA vaccines, development of vaccines, future of vaccines, advantages of vaccines, limitation of vaccines, benefits of vaccines.
This document provides information on the laboratory diagnosis of COVID-19. It discusses the types of specimens that can be collected for testing, including nasopharyngeal swabs, sputum, and blood. The main tests for diagnosis are reverse transcriptase PCR tests, which detect viral RNA in specimens. Serological tests that detect antibodies are also used but are not recommended for diagnosis. Factors that can lead to false negative PCR tests are also reviewed, such as inadequate specimen collection or testing outside the diagnostic window. Hematological and biochemical parameters that may be seen in COVID-19 patients are summarized.
This document provides an overview of vaccines including:
- A brief history of vaccines from Edward Jenner's smallpox vaccine to Louis Pasteur's rabies vaccine.
- Descriptions of different types of vaccines including whole organism, subunit, DNA, recombinant vector, and edible vaccines.
- Discussions of immunization programs, challenges in developing an HIV vaccine, the debated link between the MMR vaccine and autism, and the goal of developing more effective and accessible vaccines worldwide.
The document summarizes the replication process of DNA and viruses. It describes how DNA replicates semi-conservatively to produce two identical copies. Viruses like bacteriophage T4 and plant virus TMV are then discussed, with T4 exhibiting a lytic cycle where it hijacks the host cell to produce new virus particles that ultimately burst the cell, while TMV has an RNA genome and protein coat. The stages of viral replication including attachment, penetration, replication of the genome, and assembly of new virus particles are also outlined.
The document discusses anti-viral chemotherapy and considerations for developing effective anti-viral drugs. It notes that ideal drugs are selectively toxic to virus-infected cells, inhibit specific viral enzymes or functions, and have a high therapeutic index. Several classes of anti-viral drugs are described including nucleoside analogs that target viral polymerases, protease inhibitors for HIV, and neuraminidase inhibitors for influenza. Developing drugs that can interfere with viral replication without harming host cells remains an ongoing challenge.
INTRODUCTION OF GENE THERAPY, HISTORY OF GENE THERAPY, Process of gene therapy, Methods of gene therapy, Ex vivo gene therapy , In Vivo Gene Therapy , Uses of gene therapy, Target sites for Gene Therapy , Vectors for gene therapy , Viral Vectors, Non Viral Vectors,
The document outlines the general methods used for vaccine production, which includes 5 main steps: 1) generating the antigen by growing pathogens, 2) releasing and isolating the antigen from growth materials, 3) purifying the antigen using various separation techniques, 4) adding other components like adjuvants and preservatives, 5) packaging the vaccine for distribution while protecting it and the environment. Vaccines work by improving immunity but may not guarantee full protection against disease.
Fungi are eukaryotic organisms that can be single-celled or multicellular. Their cell walls contain chitin and glucans. Fungi can cause superficial infections of the skin and nails, subcutaneous infections beneath the skin, and systemic infections of internal organs. Common fungal diseases include ringworm, athlete's foot, histoplasmosis, and coccidiomycosis. Fungi are classified based on their modes of reproduction and microscopic structures. Identification and diagnosis involves microscopic examination, culturing, and serological tests.
Systemic mycoses can result from inhalation of fungal spores that then differentiate into yeast or other forms in the lungs. This document focuses on four specific systemic mycoses: Coccidioides, Histoplasma, Blastomyces, and Paracoccidioides. Coccidioides causes valley fever through inhalation of spores in dry soil in the southwestern US and Central/South America. Paracoccidioides causes a similar disease through inhalation in parts of Central/South America. Both fungi exist as molds in soil and yeasts in tissues. Symptoms range from asymptomatic to disseminated disease. Diagnosis involves microscopy, culture, and ser
The document discusses two systemic mycoses - Coccidioides and Paracoccidioides. Both are dimorphic fungi that exist as molds in soil and yeasts in tissue. They are inhaled through spores and can cause asymptomatic pulmonary infection or disseminated disease. Coccidioides is endemic to parts of the Americas while Paracoccidioides is found in parts of Latin America. Diagnosis involves microscopy, culture, and serology of tissues or body fluids. Treatment involves antifungal drugs like amphotericin B and itraconazole.
1. Cutaneous mycoses are fungal infections of the skin, hair, and nails caused by dermatophytes like Trichophyton, Microsporum, and Epidermophyton. Laboratory diagnosis involves microscopic examination of skin scrapings or nail clippings in KOH to identify fungal elements, as well as fungal culture.
2. Subcutaneous mycoses involve fungal infection of the subcutaneous tissue and overlying skin, such as mycetoma, chromoblastomycosis, sporotrichosis, and rhinosporidiosis. They are caused by a heterogeneous group of fungi introduced through the skin via minor trauma.
The main aim of Corona is transmission of disease from person to person, and it had also been declared as a global pandemic which has caused disaster in the respiratory system of more than five million people and killed more than half a billion people across the world. Patients surviving from Covid-19 have lower immunosuppressive CD4+ T and CD8+ T Cells. And most of the patients are in severe need of mechanical ventilation. This is the reason for a longer stay in hospital for a particular patient. Gradually, these patients have been discovered to develop fungal co-infection. This infection is deadly leading to loss of hearing, loss of sight and eventually death. The fungal infection is referred to as Mucormycosis, the black fungus. The causative agent for this infection is Mucormycotina which is a member of Mucorales. Mucormycotina usually habitats in soil and decaying organic matter. The infection of Mucormycotina is associated with a wide range of human diseases including arthritis, gastritis, renal disorders and pulmonary diseases. This infection is closely associated with the mucous layer of skin, precisely cutaneous layer. This infection is present in the nasal and upper respiratory tract. In the lower respiratory tract these infections are difficult to diagnose and treat due to the lack of precise methods. It was found those neutroponia patients are more
prone to this infection. This is caused by extensive use of chemotherapy resulting in impaired immunity. In recent times, in the case of pulmonary Mucormycosis, necrotizing pneumonia is a major symptom. A combination of antifungal and antimicrobial
agents is being used for a higher clinical recovery in the Mucormycosis case.
Candidiasis is an infection caused by Candida species, which are normally present on human skin and mucous membranes. When the normal bacterial flora is disrupted by antibiotics, Candida can overgrow and cause opportunistic infections. Common manifestations include oral thrush, vaginal candidiasis, and skin rashes. Candidiasis is diagnosed by visualizing budding yeast and pseudohyphae on smears or growing Candida species in culture. Identification tests help determine the specific Candida species involved. Immunocompromised individuals are more susceptible to severe, disseminated forms of candidiasis.
This document provides information on the morphology, properties, classification, and pathogenicity of mycobacteria. It discusses Mycobacterium tuberculosis and Mycobacterium leprae, the causative agents of tuberculosis and leprosy, respectively. It also covers atypical (nontuberculous) mycobacteria (MOTT). Mycobacteria are acid-fast, aerobic bacteria that are often slow-growing and can survive intracellularly. M. tuberculosis causes tuberculosis through airborne transmission, while M. leprae causes leprosy via prolonged skin contact. Both can have varied clinical presentations depending on host immunity. MOTT are opportunistic pathogens typically found in the environment that can cause disease in immunocomp
This document discusses several types of fungal infections. It describes the characteristics of fungi and how they can cause superficial infections like ringworm or deeper infections in immunocompromised individuals. It provides details on specimen collection and diagnostic tests for fungi. Common fungal infections discussed include candidiasis, dermatophytosis, mucormycosis, and aspergillosis. Treatment involves topical and oral antifungal medications.
1. Darrell and his puppy Delbert both developed skin infections on their lips and muzzles after Darrell gave Delbert frequent kisses.
2. They likely contracted a zoophilic dermatophyte such as Microsporum canis from Delbert to Darrell via dog-to-human transmission.
3. The diagnosis would be made by examining skin and hair scrapings under a microscope after applying potassium hydroxide or calcofluor white.
The document provides an overview of medical mycology and fungal infections. It discusses different types of fungal infections including superficial mycoses that do not involve tissue response, cutaneous mycoses caused by dermatophytes that infect the skin, subcutaneous mycoses caused by soil fungi, and systemic or opportunistic mycoses that affect immunocompromised individuals. Common fungal infections like candidiasis, aspergillosis, cryptococcosis and zygomycosis are explained in terms of their causative agents and presentations. The diagnosis and treatment of various mycoses is also covered briefly.
The document discusses types of fungal infections and the immune response against them. It outlines 5 types of fungal infections: superficial, cutaneous, subcutaneous, systemic, and opportunistic. Superficial infections involve the outer skin layer and are usually caused by yeasts. Cutaneous infections involve deeper skin layers and elicit an immune response, such as ringworm. The document then briefly describes the innate and adaptive immune responses to fungal infections.
The document discusses various viral, bacterial, fungal and protozoal infections that affect the skin. It provides details on chickenpox, shingles, measles, rubella, smallpox and warts which are viral infections. Bacterial infections covered include acne, anthrax, gas gangrene, leprosy and various Staphylococcus and Streptococcus infections. Fungal infections discussed are various types of ringworm. Lastly, it mentions leishmaniasis as a protozoal infection. For each infection, it provides information on causative agents, transmission, signs/symptoms and diagnosis.
Fungal Infections/ Mycoses ppt by Dr.C.P.PRINCEDR.PRINCE C P
PPT prepared by :
DR.PRINCE C P
Associate Professor &HOD
Department of Microbiology,
Mother Theresa Post Graduate & Research Institute of Health Sciences (Government of Puducherry Institution)
According to tissue involved, MYCOSES are classified into:
Superficial (Surface )
Cutaneous
Subcutaneous
Deep Cutaneous
Systemic (Primary )
Systemic ( Opportunistic)
Mycotic Poisoning
most of the fungal infections are opportunistic in nature.
candida albicans is the common Fungal pathogen.
The document provides information on Mycobacterium tuberculosis, which causes tuberculosis. It discusses the morphology, identification, pathology, and laboratory diagnosis of M. tuberculosis. It also covers how M. tuberculosis infects the lungs and spreads systemically, evades the immune system, and can be diagnosed via a tuberculin skin test. Treatment typically involves a long course of multiple antibiotics like rifampicin and isoniazid to prevent resistance.
Staphylococcus aureus and Streptococcus pyogenes are common Gram-positive bacterial infections that cause issues like skin lesions, pneumonia, and rheumatic fever. They produce toxins and adhere to host cells to cause illness. Gram-negative bacteria like Neisseria meningitidis, Bordetella pertussis, and Pseudomonas aeruginosa can lead to meningitis, whooping cough, and pneumonia through virulence factors and invasion of tissues. Yersinia pestis caused the bubonic plague through proliferation in lymph nodes and necrosis of tissues.
This document discusses the aerobic actinomycete Nocardia. It begins by classifying Nocardia taxonomically and noting it is an opportunistic pathogen found in soil. The document then covers the epidemiology, pathogenesis, clinical manifestations including pulmonary, skin and disseminated infections, diagnosis through microscopy, culture and molecular methods, and treatment with antimicrobials. In summary, Nocardia is an environmental actinomycete that can cause infection in immunocompromised individuals, with pulmonary disease being most common and diagnosis relying on microscopy, culture and molecular identification methods.
This document provides an overview of pathogenic cocci bacteria, focusing on Staphylococcus aureus, Neisseria gonorrhoeae, and Neisseria meningitidis. It describes the characteristics, epidemiology, pathogenesis, clinical manifestations, diagnosis, and treatment of infections caused by these bacteria. Key points include that S. aureus is a common cause of skin and soft tissue infections, N. gonorrhoeae causes the sexually transmitted infection gonorrhea, and N. meningitidis can cause meningitis and sepsis.
This document discusses diseases caused by various microorganisms including bacteria, fungi, and parasites. It provides details on staphylococcal infections including those caused by Staphylococcus aureus and the wide range of clinical manifestations. It also discusses streptococcal infections focusing on different disease-causing species and their associated diseases. Finally, it covers clostridial diseases including gas gangrene caused by Clostridium perfringens and tetanus caused by Clostridium tetani.
This document discusses various systemic mycoses (fungal infections of internal organs) including histoplasmosis, blastomycosis, coccidioidomycosis, and paracoccidioidomycosis. It describes the causative fungi, how infection occurs through inhalation of spores, clinical features involving the respiratory system and dissemination, laboratory diagnosis using microscopy, culture, and immunodiagnosis, and treatment involving antifungal drugs. Candidiasis is also discussed as the most common fungal infection affecting mucosa and internal organs in immunocompromised individuals.
Drug resistance occurs when microbes develop the ability to survive exposure to drugs like antibiotics that previously could kill them. It happens because of overuse and misuse of antibiotics in humans and livestock as well as lack of development of new drugs. This reduces the effectiveness of antibiotics and makes diseases harder to treat, increasing healthcare costs and risk of death. Key strategies to prevent further drug resistance include only using antibiotics when needed, always taking the full prescribed dose, and improving infection control and hygiene.
Mycobacteria are slender rod-shaped bacteria that can be stained using special techniques. The most important mycobacteria that cause disease are M. tuberculosis, which causes tuberculosis (TB), and M. leprae, which causes leprosy. Both bacteria are acid-fast rods that form colonies when cultured. M. leprae cannot be cultured in vitro. Both bacteria cause granulomatous lesions in humans and evade the immune system through waxy coatings and inhibiting phagosome-lysosome fusion in macrophages. Diagnosis involves microscopy, culture, PCR and skin testing. Treatment includes antibiotics.
This document discusses hypersensitivity, classifying it into four types based on mechanisms. Type 1 is anaphylaxis caused by IgE antibodies binding to mast cells. Type 2 is antibody-dependent cytotoxicity. Type 3 involves immune complex deposition in tissues. Type 4 is cell-mediated delayed hypersensitivity. The types are distinguished by their pathophysiology and examples are given of conditions that fall under each type along with associated symptoms and treatments.
Hemolytic disease of the newborn (HDN), also called erythroblastosis fetalis, is a blood disorder that occurs when the mother and baby have incompatible blood types. During pregnancy, the baby's red blood cells can enter the mother's bloodstream through the placenta. If the mother's immune system sees the baby's blood type as foreign, it will produce antibodies to attack the baby's red blood cells. After birth, these antibodies cause the baby's red blood cells to break down too quickly. Symptoms of HDN include jaundice, swelling, enlarged liver or spleen, and yellowing of bodily fluids. Treatment may involve phototherapy, medications, transfusions, or in severe cases, an
An ELISA uses antibodies to detect the presence of antigens in biological samples immobilized on a microplate. There are four main types of ELISA - direct, indirect, sandwich, and competitive - which differ in how the antigen is immobilized and detected. ELISA relies on the specific binding of antibodies to antigens, and can detect the target analyte using enzymatic or fluorescent tags to provide a quantifiable signal. It is a versatile technique that allows multiple samples to be tested simultaneously.
1. This document provides an overview of a clinical chemistry course, outlining its aims, objectives, and topics to be covered including the scope of clinical chemistry, specimen requirements, and quality assurance principles.
2. It reviews the history of clinical chemistry from early experiments isolating compounds from living materials in the 19th century to modern developments in instrumentation, automation, and computer integration that have increased testing efficiency and productivity.
3. Advances in clinical chemistry have expanded biochemical knowledge and allowed for more accurate detection of metabolic disorders through small sample sizes and short test turnaround times, supporting evidence-based medicine.
This document discusses blunt trauma injuries, including definitions, classifications, signs, symptoms, and methods of diagnosis. It describes different types of blunt trauma injuries like abrasions, contusions, lacerations, and fractures. It explains that blunt trauma can cause injury to internal organs like the spleen, liver, kidneys, and intestines. Imaging tests and diagnostic procedures help physicians evaluate and diagnose internal injuries from blunt force impacts. The document outlines investigation procedures for cases involving blunt trauma death, including examination of injury patterns, trace evidence, and scene findings.
Blood coagulation is the process by which blood changes from a liquid to a gel-like mass. It involves several coagulation factors and occurs in three stages: formation of prothrombin activator, conversion of prothrombin to thrombin, and conversion of fibrinogen to fibrin. The resulting blood clot traps platelets, red blood cells, and white blood cells. Over time, the clot retracts as platelets contract. Fibrinolysis then breaks down the clot. Coagulation ensures wounds are sealed but can become problematic if clots form inside blood vessels.
Rickettsia are obligate intracellular parasites that infect arthropods like ticks and mites. They are transmitted to humans via arthropod bites and infect endothelial cells. This causes thrombosis and occlusion of blood vessels, leading to organ damage and acute febrile illness with rash. Some important Rickettsia species that cause disease in humans are R. prowazekii (epidemic typhus), R. typhi (murine typhus), and R. rickettsii (Rocky Mountain spotted fever). Diagnosis involves microscopy, culture, serology like the Weil-Felix test or immunofluorescence antibody technique. Treatment is with doxycycline or tetracycline.
Chlamydia are small, gram-negative bacteria that are obligate intracellular parasites. They include C. trachomatis, C. psittaci, and C. pneumoniae. C. trachomatis can cause trachoma (the world's leading cause of preventable blindness), and genital infections leading to pelvic inflammatory disease. It has different biovars that cause different diseases. C. psittaci infects birds and mammals. Laboratory diagnosis includes culturing, antigen detection, PCR, and serology. Treatment is usually with tetracyclines or azithromycin. Control involves treatment, contact tracing, hygiene and avoiding animal contact.
This document provides an overview of immunodeficiency. It begins by defining immunodeficiency and classifying it into primary and secondary types. Primary immunodeficiency is due to genetic defects present at birth, while secondary immunodeficiency is acquired from other diseases, infections, or medications. The document further discusses specific deficiencies in B cells, T cells, complement systems, and phagocytes. It also covers severe combined immunodeficiency and common secondary causes like infections, malnutrition, and immunosuppressive drugs or therapies. Treatment options include supportive therapies with antibiotics or immunoglobulin replacement as well as definitive therapies like bone marrow transplantation or gene therapy.
This document discusses immunodeficiency, including:
- It defines immunodeficiency and classifies it as either specific or non-specific, as well as primary or secondary.
- It describes various types of primary immunodeficiencies affecting B cells like X-linked agammaglobulinemia and common variable immunodeficiency. It also covers T cell deficiencies like DiGeorge syndrome.
- Severe combined immunodeficiency is discussed as being either X-linked or autosomal and resulting in an absence of both T and B cells.
- Secondary immunodeficiencies can be caused by drugs like corticosteroids, methotrexate, and cyclosp
The cell membrane is a lipid bilayer composed of phospholipids and cholesterol that surrounds the cell. Integral proteins pass through the lipid bilayer, serving functions like passive diffusion of substances, active transport against gradients, and acting as receptors. Peripheral proteins are attached to the inner surface of the membrane and function as enzymes catalyzing chemical reactions. The cell membrane and organelle membranes form a barrier controlling what enters and exits the cell while integral proteins allow selective transport of substances into and out of the cell.
This document summarizes blood groups and their importance in blood transfusions. It describes Karl Landsteiner's discovery of the ABO blood group system and the corresponding antigens (A, B) and antibodies (anti-A, anti-B). Based on the presence or absence of these antigens, blood is categorized into A, B, AB, and O groups. Compatibility is required to prevent hemolytic transfusion reactions. It also covers the Rh blood group system and hemolytic disease in newborns when an Rh-negative mother has an Rh-positive baby.
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...Dr. Vinod Kumar Kanvaria
Exploiting Artificial Intelligence for Empowering Researchers and Faculty,
International FDP on Fundamentals of Research in Social Sciences
at Integral University, Lucknow, 06.06.2024
By Dr. Vinod Kumar Kanvaria
বাংলাদেশের অর্থনৈতিক সমীক্ষা ২০২৪ [Bangladesh Economic Review 2024 Bangla.pdf] কম্পিউটার , ট্যাব ও স্মার্ট ফোন ভার্সন সহ সম্পূর্ণ বাংলা ই-বুক বা pdf বই " সুচিপত্র ...বুকমার্ক মেনু 🔖 ও হাইপার লিংক মেনু 📝👆 যুক্ত ..
আমাদের সবার জন্য খুব খুব গুরুত্বপূর্ণ একটি বই ..বিসিএস, ব্যাংক, ইউনিভার্সিটি ভর্তি ও যে কোন প্রতিযোগিতা মূলক পরীক্ষার জন্য এর খুব ইম্পরট্যান্ট একটি বিষয় ...তাছাড়া বাংলাদেশের সাম্প্রতিক যে কোন ডাটা বা তথ্য এই বইতে পাবেন ...
তাই একজন নাগরিক হিসাবে এই তথ্য গুলো আপনার জানা প্রয়োজন ...।
বিসিএস ও ব্যাংক এর লিখিত পরীক্ষা ...+এছাড়া মাধ্যমিক ও উচ্চমাধ্যমিকের স্টুডেন্টদের জন্য অনেক কাজে আসবে ...
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...PECB
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
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Training: ISO/IEC 27001 Information Security Management System - EN | PECB
ISO/IEC 42001 Artificial Intelligence Management System - EN | PECB
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Main Java[All of the Base Concepts}.docxadhitya5119
This is part 1 of my Java Learning Journey. This Contains Custom methods, classes, constructors, packages, multithreading , try- catch block, finally block and more.
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
A workshop hosted by the South African Journal of Science aimed at postgraduate students and early career researchers with little or no experience in writing and publishing journal articles.
How to Make a Field Mandatory in Odoo 17Celine George
In Odoo, making a field required can be done through both Python code and XML views. When you set the required attribute to True in Python code, it makes the field required across all views where it's used. Conversely, when you set the required attribute in XML views, it makes the field required only in the context of that particular view.
This presentation was provided by Steph Pollock of The American Psychological Association’s Journals Program, and Damita Snow, of The American Society of Civil Engineers (ASCE), for the initial session of NISO's 2024 Training Series "DEIA in the Scholarly Landscape." Session One: 'Setting Expectations: a DEIA Primer,' was held June 6, 2024.
2. Zarqa Private UniversityBiology
4223 – The Fungi
Fungal Diseases
Mycosis- fungal infection
< 100 cause human disease
Not highly contagious
Humans acquire from nature
Groups based on degree on tissue involvement
and mode of entry
Cutaneous mycoses-dermatophytes
Epidermis, hair & nails
Contagious-direct or indirect contact
Secrete keratinase that degrades keratin
3. Zarqa Private UniversityBiology
4223 – The Fungi
Cutaneous Mycoses
Tinea( worm) capitis –blisters with scaly ring
Ringworm of the scalp
Spreads circularly forming bald spots
Spread by contact with fomites , cats and
dogs
Tinea cruris- ringworm of groin
Tinea pedis - athlete's foot
4. Zarqa Private UniversityBiology
4223 – The Fungi
Systemic Mycoses
Dimorphic fungi
Yeast form is invasive
Can spread throughout body
Usually caused by fungi in soil
Inhalation of spores
Begins in lungs and spread to rest of
body
Not contagious person to person
5. Zarqa Private UniversityBiology
4223 – The Fungi
Introduction
Obligate Parasitic Fungi
(dermatophytes): evolved to attack the
outer surface of humans
Facultative soil fungi: thermal
dimorphic saprobes, adaptations to
human body
Opportunistic saprobes: attack people
with compromised immune systems
6. Zarqa Private UniversityBiology
4223 – The Fungi
Introduction
Fungal Infections
Superficial infections: involve outermost
layers of skin and its appendages [ nails or
hair] ( Dermatophytosis)
Cutaneous infections: involve deeper layers
of skin causing allergic or inflammatory
response
Subcutaneous infections: fungi with low
virulence, localized infection, or spread by
mycelial growth
8. Zarqa Private UniversityBiology
4223 – The Fungi
Mycoses: diseases cause by
fungi
Superficial Cutaneous
Subcutaneous Systemic
Opportunistic
9. Zarqa Private UniversityBiology
4223 – The Fungi
The Situation
Frequency
- most common fungal pathogen worldwide
- 4th leading causes of nosocomial infections, 40% mortality
- significant mortality and morbidity in low birth-weight infants
- affects 75% women, 45% experience recurrence
> 10 million visits/year
- classified as a STD by CDC
Immunocompromised
- cancer and HIV-AIDs patientsC
- most commonly manifested in patients with leukemia
or HIV-AIDs infections. Oral candidiasis is often a
clue to acute primary infectionC
Public Concerns
- increasing resistance to drug therapies due to antibiotics and
antifungals
10. Zarqa Private UniversityBiology
4223 – The Fungi
FUNGAL DISEASES
Mycosis: Any fungal disease. Tend to be chronic because fungi
grow slowly.
Mycoses are classified into the following categories:
I. Systemic mycoses: Fungal infections deep within the body.
Can affect a number if tissues and organs.
Usually caused by fungi that live in the soil and are inhaled.
Not contagious.
Examples:
Histoplasmosis (Histoplasma capsulatum): Initial
infection in lungs. Later spreads through blood
to most organs.
Coccidiomycosis (Coccidioides immites):
Resembles tuberculosis.
12. Zarqa Private UniversityBiology
4223 – The Fungi
FUNGAL DISEASES (Continued)
II. Cutaneous mycoses: Fungal infections of the skin, hair, and
nails.
Secrete keratinase, an enzyme that degrades keratin.
Infection is transmitted by direct contact or contact with
infected hair (hair salon) or cells (nail files, shower floors).
Examples:
Ringworm (Tinea capitis and T. corporis)
Athlete’s foot (Tinea pedis)
Jock itch (Tinea cruris)
18. Zarqa Private UniversityBiology
4223 – The Fungi
FUNGAL DISEASES (Continued)
III. Subcutaneous mycoses: Fungal infections beneath the
skin.
Caused by saprophytic fungi that live in soil or on vegetation.
Infection occurs by implantation of spores or mycelial
fragments into a skin wound.
Can spread to lymph vessels.
IV. Superficial mycoses: Infections of hair shafts and
superficial epidermal cells. Prevalent in tropical climates.
19. Zarqa Private UniversityBiology
4223 – The Fungi
FUNGAL DISEASES (Continued)
Opportunistic mycoses: Caused by organisms that are
generally harmless unless individual has weakened defenses:
AIDS and cancer patients
Individuals treated with broad spectrum
antibiotics
Very old or very young individuals (newborns).
Examples:
Aspergillosis: Inhalation of Aspergillus spores.
Yeast Infections or Candidiasis: Caused mainly
by Candida albicans. Part of normal mouth,
esophagus, and vaginal flora.
20. Zarqa Private UniversityBiology
4223 – The Fungi
Cutaneous Infections
Dermatophytic hyphomycetes
40 species
Epidermophyton (2 species)
Microsporum (17 species)
Trichophyton (24 species)
50% of dermatophytes human specific
21. Zarqa Private UniversityBiology
4223 – The Fungi
Cutaneous Infections
Cause common tinea (ringworm)
Grow only on humans
Reservoir not in soil or animals
Reservoir in carpets and upholstery
for up to two years
23. Zarqa Private UniversityBiology
4223 – The Fungi
Tinea corporis
Subcutaneous mycoses
Subcutaneous infections - over 35
species produce chronic inflammatory
disease of subcutaneous tissues and
lymphatics. e.g. sporotrichosis -
ulcerated lesions at site of inoculation
followed by multiple nodules - caused
by a dimorphic fungus: Sporotrix
schenckii.
24. Zarqa Private UniversityBiology
4223 – The Fungi
Cutaneous Infections
Microsporum canis
Reservoir in cat
May move to humans or dogs
Dies out after one or two person-
person transfers
25. Zarqa Private UniversityBiology
4223 – The Fungi
Cutaneous Infections
Disease process
Fungus stimulates epithelial cells of
skin to divide more frequently
Makes more keratin available to
fungus
Some species race specific in humans
Some species body location specific
26. Zarqa Private UniversityBiology
4223 – The Fungi
Cutaneous Infections
Candida albicans – candidiasis
Normal component of gut mycota
Excessive wetness overgrowth on
skin
Vaginal candidiasis common in
pregnant women
31. Zarqa Private UniversityBiology
4223 – The Fungi
Genital Yeast Candidiasis
Symptoms
Risk Factors
- disruption of normal
microbiota
Treatment
- direct genital administration
- tablets, suppositories, creams
Figure 1. Vaginal Yeast Culture
Figure 2. Plasma cell balanitis. A
band-like infiltrate of plasma cells is in
the dermis of the male penis.
33. Zarqa Private UniversityBiology
4223 – The Fungi
Pathogenesis
Host Recognition
Adhesins
Enzymes
Hydrolases: Phosphoplipases, Lipases, Proteinases
Morphogenesis
Yeast form to Filamentous hyphae/pseudohyphae
Phenotypic Switching
34. Zarqa Private UniversityBiology
4223 – The Fungi
Figure 1. skin equivalent before infection
Figure 2. Infection with pathogenic clinical isolate of C. albicans.
After 48 h the yeast penetrates the skin equivalent and destroys
the tissue
Figure 3. Infection with non-pathogenic C. albicans. This strain is not
able to penetrate into the tissue and thus behaves as avirulent as shown
in the mouse model of systemic infection.
Virulence assay of different C. albicans strains using the skin equivalent (AST 2000)
(Fraunhofer, 2002)
35. Zarqa Private UniversityBiology
4223 – The Fungi
MORPHOGENESIS
Figure 2. Morphogenic forms of Candida albicans
http://cbr-rbc.nrc-cnrc.gc.ca/thomaslab/candida/caindex.html
Figure 1. Morphogenesis.
Morphogenesis in
C. albicans is a pivotal
virulence factor that allows
rapid multiplication and
subsequent dissemination
in host tissue.
(www.kent.ac.uk)
36. Zarqa Private UniversityBiology
4223 – The Fungi
Subcutaneous Infections
Fungal Activity
Fungi normally saprobic
Introduced through wounds
Adapt to the human animal by changes in
morphology
physiology
37. Zarqa Private UniversityBiology
4223 – The Fungi
Subcutaneous Infections
Chromoblastosis
Common among barefoot peoples of the
tropics
Soil hyphomycete species
Enters human by thorns or wood slivers
Fungus grows host cells respond by
rapid cell division wart-like growths
on feet or legs
38. Zarqa Private UniversityBiology
4223 – The Fungi
Subcutaneous Infections
Mycotic Mycetoma
Disease of barefoot tropical people
Entry: wound on foot
Attacks various tissues
Stimulates formation of tumor
Compact fungal colonies form within
tumor
43. Zarqa Private UniversityBiology
4223 – The Fungi
Systemic Mycoses
Coddidioidomycosis
Coccidioides immitis
Dry, saline soils
Endemic to SW deserts of North
America
Valley fever
Until recently – almost always fatal
44. Zarqa Private UniversityBiology
4223 – The Fungi
Systemic Mycoses
Infection, disease process, and clinical
symptoms similar to histoplasmosis
Can be effectively treated with
fluconazole
45. Zarqa Private UniversityBiology
4223 – The Fungi
Opportunistic Pathogens/Disease
General
Pathogens all grow well at 37C
None cause disease in well individuals
Require breakdown in resistance system
Complication of diabetes, AIDS,
advanced cancer, sequel to steroid or
antibiotic treatments
46. Zarqa Private UniversityBiology
4223 – The Fungi
Opportunistic Pathogens/Disease
Zygomycosis
Species of Zygomycota
Rhizopus, Mucor, Rhizomucor
Rhinocerebral mycosis
Spores enter through sinuses
Grows rapidly outward to the eyes and
inward towards the brain
47. Zarqa Private UniversityBiology
4223 – The Fungi
Opportunistic Pathogens/Disease
Aspergillosis
Aspergillus sp.
Bronchiopulmonary aspergillosis
Mucus within the bronchi severe
allergic reaction
Aspergilloma
Forms a mycelia ball in lung cavity
formed from earlier TB
48. Zarqa Private UniversityBiology
4223 – The Fungi
Opportunistic Pathogens/Disease
Surgical intervention often required
Invasive aspergillosis
Severely debilitated
Immunosuppressed (AIDS)
Almost always fatal until recently
49. Zarqa Private UniversityBiology
4223 – The Fungi
AIDS and Mycoses
Aspergillosis
Candidiases (Candidiasis seen in 2/3 of
AIDS patients
Cryptococcosis
Zygomycosis
Esophogeal candidiasis and
cryptococcosis are strong indicators of
AIDS