This document provides information on the laboratory diagnosis of tuberculosis. It discusses the classification of mycobacteria, specimen collection, and the various diagnostic methods used which include smear microscopy, culture, and molecular tests. Smear microscopy has limitations but is widely used due to its low cost. Culture is the gold standard but is more complex and requires biosafety. Liquid culture systems allow for faster results than solid media. Drug sensitivity testing determines resistance and is important for treatment. Molecular tests like line probe assays and GeneXpert can rapidly detect M. tuberculosis and resistance, with GeneXpert suitable to test pulmonary and some extrapulmonary samples directly. The document concludes with details about Microcare Laboratory which provides accredited tuberculosis diagnostic services
Catridge based nucleic acid amplification test(CBNAAT) / RIF assay gene xpert POWER PONT. other normal tests versus CBNAAT. issues for cbnaat by WHO & CONCLUSION.
The PPT is mainly all about Mycobacterium Tuberculosis. Agents causing the disease Tuberculosis, pathogenesis, laboratory diagnosis, treatment and prophylaxis. It was made for both BSc and MSc students.
RECENT ADVANCES IN DIAGNOSIS OF TUBERCULOSISANGAN KARMAKAR
TRADITIONAL TESTS AND RECENT DIAGNOSTIC MODALITIES FOR TUBERCULOSIS WITH EMPHASIS TO MOLECULAR DETECTION TECHNIQUES, DRUG SENSITIVITY ASSESMENT IN INDIAN PERSPECTIVE
Catridge based nucleic acid amplification test(CBNAAT) / RIF assay gene xpert POWER PONT. other normal tests versus CBNAAT. issues for cbnaat by WHO & CONCLUSION.
The PPT is mainly all about Mycobacterium Tuberculosis. Agents causing the disease Tuberculosis, pathogenesis, laboratory diagnosis, treatment and prophylaxis. It was made for both BSc and MSc students.
RECENT ADVANCES IN DIAGNOSIS OF TUBERCULOSISANGAN KARMAKAR
TRADITIONAL TESTS AND RECENT DIAGNOSTIC MODALITIES FOR TUBERCULOSIS WITH EMPHASIS TO MOLECULAR DETECTION TECHNIQUES, DRUG SENSITIVITY ASSESMENT IN INDIAN PERSPECTIVE
This is a presentation giving an overview of the GeneXpert DX system for detection of MTB. The assay described in this presentation is the MTB/RIF test.
"What Will It Take To Control TB?" Richard Chaisson, MDUWGlobalHealth
Dr. Richard Chaisson, Professor of Medicine, Epidemiology and International Health and Director of the Center for Tuberculosis Research at the Johns Hopkins University in Baltimore was the keynote Jan. 19 as part of the Washington Global Health Discovery Series. His talk was on ""What Will It Take To Control TB?"
Abdominal Tuberculosis – How Far are Our Diagnostics Illuminating?Apollo Hospitals
Tuberculosis can involve any part of the gastrointestinal tract from mouth to anus, the peritroneum, pancreas and the hepatobiliary system. Gastrointestinal tuberculosis mimics many clinical conditions and only a high degree of suspicion can help in the diagnosis otherwise there are chances of missing it leading to high morbidity and mortality. Various methods of diagnosis are available but which one is the right test for a particular patient needs to be ascertained. Culture remains the gold standard method of diagnosis. Fast track cultures like MGIT/M Bact Alert 3 D can give faster results with in few days to few weeks. Molecular tests are fastest and can be used as a supplementary test. Nested PCR can give results with in few hours.
Comparison of Ziehl Neelsen Microscopy with GeneXpert for Detection of Mycoba...iosrjce
IOSR Journal of Dental and Medical Sciences is one of the speciality Journal in Dental Science and Medical Science published by International Organization of Scientific Research (IOSR). The Journal publishes papers of the highest scientific merit and widest possible scope work in all areas related to medical and dental science. The Journal welcome review articles, leading medical and clinical research articles, technical notes, case reports and others.
This is a research article presentation. I have prepared an original article for power point presentation, it will be helpful for you all to know how to present an original article on journal club.
Before doing research in any field it is very important to know the way of writing a research article . We should know which different points to remember at the time of research paper presentation .I have included all the headings which fulfill all the demands of a better way to present a research article on journal club.
Similar to Laboratory diagnosis of Tuberculosis (20)
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
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Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
2. CLASSIFICATION OF MYCOBACTERIA
Mycobacterium tuberculosis complex refers to a genetically related
groups of Mycobacterium species that can cause TUBERCULOSIS
[TB] in humans. It includes;
Mycobacterium tuberculosis,
Mycobacterium bovis [M.bovis, subsp bovis, M.bovis subsp.caprae and M.bovis BCG]
Mycobacterium africanum,
Mycobacterium microti,
Mycobacterium canetti,
Mycobacterium mungi,
Mycobacterium orygis and
Mycobacterium pinnipedii
3. SPECIMEN COLLECTION
In every clinical Microbiology sample collection
“Results are as good as Specimen” Good quality sample
is very important.
Sputum samples of good quality collected in wide mouth
sterile containers.
Quantity sufficient
Extra pulmonary samples collected in sterile containers
/syringes.
6. In low income and high tuberculosis prevalence
countries, sputum smear microscopy is the only cost-
effective tool for diagnosing patients with infectious
tuberculosis and to monitor their progress in treatment.
Sputum smear microscopy is a simple, inexpensive,
appropriate technology method which is relatively easy to
perform and to read.
Classical Ziehl-Neelsen stain used-AFB
SMEAR MICROSCOPY
7. Smear prepared from thick purulent parts of samples.
Size of smear should be 3cmx2cm
At least 300 fields examined
Smear is positive in samples which contain 5000- 10000
bacteria /ml
Sensitivity ranges from 25%-65%
Sensitivity increases by examination of more than one
smear
SMEAR MICROSCOPY
10. Smears reported as Positive or
Negative
Quantity of AFB observed
should be noted
Factors influencing smear
sensitivity are type of specimen,
staining technique, experience
of reader
Laboratory Quality Control
important
ZN STAINING
11. FLUORESCENCE MICROSCOPY
Fluorochrome stain used
Can be examined at lower magnification(40 X)
Rapid but more false positive
LED fluorescence microscopy has been evaluated- rapid
and good results, lower cost
LED attachment to microscope Primo Star iLED from Carl
Zeiss
13. DISADVANTAGES OF SMEAR
MICROSCOPY
Needs a large no of bacilli per ml of specimen to be
detected positive
Cannot differentiate between dead and live bacilli
Cannot differentiate between Mtb and NTM
No idea of drug resistance
14. AFB CULTURE
GOLD STANDARD
Provides definitive diagnosis of TB
Pure growth of mycobacteria to do speciation and drug
sensitivity.
Technically demanding and complex
High level of Biosafety needed
15. AFB CULTURE BY L.J. MEDIA [SOLID]
Detection of 10-100 viable
bacilli/ml of specimen
Specimens have to be
decontaminated before
inoculation to remove the
normal bacterial flora.
Solid culture – Conventional LJ
method.
Mycobacteria slow growing
and hence take 2-8 weeks to
grow
LOWENSTEIN JENSEN
[L.J. ] MEDIA
16. LIQUID CULTURE
Many Commercial systems available- BACTEC systems
MGIT960, BacT/ALERT 3D system
Liquid culture yield significantly rapid results than solid
media and isolation rates for mycobacteria are higher
Liquid media- Middle brook 7H9 media used
MGIT system( Mycobacterial growth indicator tubes)
contains a modified Middle brook 7H9 broth with a
fluorescence quenching based oxygen sensor. Growth of
mycobacteria leads to oxygen depletion and indicator
fluoresces brightly
Cultures positive in 10-14 days
18. DRUG SENSITIVITY FOR AFB
Sensitivity to first and second line drugs available
Expensive
High degree of technical expertise and lab infrastructure
required
Rigorous quality control needed
19. NON COMMERCIAL METHOD
MODS [Microscopic observed drug susceptibility]
A micro colony method in liquid culture , based on
inoculation of specimens into drug free and drug
containing media, followed by microscopic examination of
early growth .
Recommended as direct or indirect tests for rapid
screening of patients suspected of having MDR TB
20. CRI
( Colorimetric redox indicator)
Indirect testing methods based on the reduction of a
coloured indicator added to liquid culture medium on a
microtitre plate after exposure of M. tb strains to anti TB
drugs in vitro
21. NRA
(Nitrate reductase assay)
A direct or indirect method on solid culture based on the
ability of M. tuberculosis to reduce nitrate, which is
detected by a colour reaction
23. MOLECULAR TEST
Genotypic methods have considerable advantage of
speed, standardization of testing and reduced requirement
for Biosafety
1. LINE PROBE ASSAY
( HAINS TEST)
2. GENEXPERT
24. 1. LINE PROBE ASSAY ( HAINS TEST)
Simultaneous identification for M.tuberculosis complex
Molecular assay for the detection of resistance to INH &
RIF of M.tuberculosis complex
By detection of most significant mutations to – inhA,
RpoB and the katG genes
Based on DNA strip technology
Can be done from positive cultures (from MGIT,
BacT/ALERT bottles or LJ)
Pulmonary samples which are smear +ve can be done
directly
25. Detection of multiple genes responsible for the antibiotic
resistance &
Simultaneous recognition of missing wild type gene
Also Available for Second secondline
and identification of some strain of NTM
Limitations of Genotype MTBDRplus
Needs preprocessing of samples.
Needs a PCR set up
Technically demanding
Panic of contamination
Special infrastructure required
Needs dedicated staff and space.
26. 2. GENEXPERT [CBNAAT]
The Xpert MTB/RIF is a cartridge based nucleic acid
amplification test , automated diagnostic test that can
identify Mycobacterium tuberculosis (MTB) DNA and
resistance to Rifampicin (RIF) by Nucleic Acid
Amplification Test(NAAT).
SAMPLES;
Pulmonary samples( Sputum, BAL )
Extra pulmonary samples [Lymph node tissue and
aspirates, CSF, Pus , Gastric lavage and aspirates ( in
children) & Other Tissues]
27. Pulmonary samples - Xpert MTB/ Rif Sensitivity
Status Sensitivity %
Smear +ve culture +ve 98
Smear –ve culture +ve 68
People with HIV 79
People without HIV 86
Extra pulmonary samples Xpert MTB/Rif - sensitivity and specificity
Samples Sensitivity % Specificity %
Lymphnode tissue and
aspirate
84.9 92.5
CSF 79.5 98.6
Pleural fluid 43.7 98.1
Gastric lavage and
aspirations
83.8 98.1
Other tissue 81.2 98.1
28. ABOUT MICROCARE LABAORATORY & TRC
Microcare Laboratory was Certified by ISO 9001:2000 in the year
of 2007, First in south Gujarat.
Microcare Laboratory was accredited by NABL in the year of March
2011, first laboratory in south Gujarat to get NABL Accreditation in
the field of Microbiology.
With the fully & favorable support of STO, STATE TB CELL and
whole team of RNTCP Gujarat government, Microcare laboratory
accredited by National Mycobacteriology Accreditation system of
Central TB Division, Govt. of India. 1st and only one lab In Gujarat in
private sector for solid C&DST.
29. C&DST BY L.J. C&DST BY
Bactec MGIT
MDR [TB]
Detection by
Genotype [Line
Probe Assay].
Detection of
MTB &
Rifampicin By
GeneXpert.
& Routine Culture and sensitivity testing of all clinical samples.
FLUORESCENCE
MICROSCOPY
30. From May 2013 to Dec. 2015 More than 200
patients were Notified by Microcare
laboratory through Nikshay