Ecology of Ranaviruses: State of Understandingmgray11
Presentation on the basic ecology of ranaviruses and mechanisms that may be contribute to this pathogen's emergence. By Dr. Matt Gray (University of Tennessee, Center for Wildlife Health)
Are ranaviruses capable of causing local population extinctions and contributing to species declines? By Dr. Matt Gray (University of Tennessee, Center for Wildlife Health)
Ecology of Ranaviruses: State of Understandingmgray11
Presentation on the basic ecology of ranaviruses and mechanisms that may be contribute to this pathogen's emergence. By Dr. Matt Gray (University of Tennessee, Center for Wildlife Health)
Are ranaviruses capable of causing local population extinctions and contributing to species declines? By Dr. Matt Gray (University of Tennessee, Center for Wildlife Health)
Dr. Peter Davies - Emerging Issues in Antibiotic Resistance Linked to Use in ...John Blue
Emerging Issues in Antibiotic Resistance Linked to Use in Food Animals - Dr. Peter Davies, College of Veterinary Medicine, University of Minnesota, from the 2017 Allen D. Leman Swine Conference, September 16-19, 2017, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2017-leman-swine-conference-material
Crayfish Predation on Snails in Different Environments diontaedm
This project was ran at Kellogg Biological Station in the summer of 2015. The project was conducted questioning if snails could better survive in habitats composed of different substances. This question came about as we were searching for snails and continuously found dead snails in sandy areas and more living sands in areas with Detritus
Biotechnological applications in preserving endangered speciesDariyus Kabraji
A presentation on a few dolphin species under threat, the virus responsible for so many of their deaths, and the biotechnological undertaking to solve this problem
Citharinus citharus, Heterobranchus bidorsalis, Synodontis clarias and Heterotis niloticus were examined for Endoparasites from June to January. A sample size of 160 fish each, for the four fish species were analysed. Two species of parasites were recovered; Eustrongylides sp (Nematode) and D. latum (Cestode). Eustrongylides sp. was prevalent > 90% in all the fish species. All the parasites were recovered from the gastrointestinal tract. Synodontis clarias had the highest mean parasites load of 5.27.78±0.75 in the stomach and 2.85±0.49 in the intestine during the dry season.
Poster ISME 2012 - Gut community analysis of Atlantic Cod (Gadus morhua)Thomas Haverkamp
By far the majority of vertebrate diversity is represented by fish lineages. Nevertheless, gut communities of vertebrates have been mainly investigated in mammalian hosts despite the fact that mammals only represent a fraction of this diversity. Most fish gut communities have been studied with 16S rRNA clone libraries or culture based methods and only one pyrosequenced zebrafish gut community study – in which individual samples were pooled - is available[1,2]. Therefore, a detailed microbial community analysis of fish guts using the power of next generation sequencing on individual samples is lacking. Here we analyze pyrosequenced amplicon libraries of the gut communities of 11 Atlantic Cod (Gadus morhua) specimens caught in the outer Oslo Fjord. We find that the most abundant taxa are shared among individuals, an observation supporting the idea of the presence of a core gut microbiome in fish species.
Dealing with heterogeneous data to improve our knowledge of biodiversity dynamics and ecosystem function: perspectives from synthesis projects: presented by Liliana Ballesteros-Meija for ACTIAS (Global patterns of insect diversity, distribution and evolutionary distinctness - What can we learn from two of the best-documented families of moths?) at the sfécologie conference 2018.
more information on the group: http://www.cesab.org/index.php/fr/projets-en-cours/projets-2014/130-actias
"Estimation of Divergence Times in Asparagales in the Presence of Hybridization," presented in symposium "Insights and Benefits from Monocot Palaeobiology: Fossils, DNA, and Phylogenies" at Monocots V (5th International Conference on Comparative Biology of Monocotyledons, The New York Botanical Garden, July 2013).
Los días 7 y 8 de mayo organizamos en la Fundación Ramón Areces con la Fundación General CSIC el Simposio Internacional 'Microbiología: transmisión'. La "transmisión" en microbiología hace referencia al proceso por el que material genético es transferido de una célula a otra, de una población a otra. Es un proceso clave para entender el origen y la evolución de los seres vivos. El objetivo de esta reunión era conocer mejor la logística de la transmisión para ser capaces de modular o suprimir algunos procesos de transmisión dañinos.
Dr. Peter Davies - Emerging Issues in Antibiotic Resistance Linked to Use in ...John Blue
Emerging Issues in Antibiotic Resistance Linked to Use in Food Animals - Dr. Peter Davies, College of Veterinary Medicine, University of Minnesota, from the 2017 Allen D. Leman Swine Conference, September 16-19, 2017, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2017-leman-swine-conference-material
Crayfish Predation on Snails in Different Environments diontaedm
This project was ran at Kellogg Biological Station in the summer of 2015. The project was conducted questioning if snails could better survive in habitats composed of different substances. This question came about as we were searching for snails and continuously found dead snails in sandy areas and more living sands in areas with Detritus
Biotechnological applications in preserving endangered speciesDariyus Kabraji
A presentation on a few dolphin species under threat, the virus responsible for so many of their deaths, and the biotechnological undertaking to solve this problem
Citharinus citharus, Heterobranchus bidorsalis, Synodontis clarias and Heterotis niloticus were examined for Endoparasites from June to January. A sample size of 160 fish each, for the four fish species were analysed. Two species of parasites were recovered; Eustrongylides sp (Nematode) and D. latum (Cestode). Eustrongylides sp. was prevalent > 90% in all the fish species. All the parasites were recovered from the gastrointestinal tract. Synodontis clarias had the highest mean parasites load of 5.27.78±0.75 in the stomach and 2.85±0.49 in the intestine during the dry season.
Poster ISME 2012 - Gut community analysis of Atlantic Cod (Gadus morhua)Thomas Haverkamp
By far the majority of vertebrate diversity is represented by fish lineages. Nevertheless, gut communities of vertebrates have been mainly investigated in mammalian hosts despite the fact that mammals only represent a fraction of this diversity. Most fish gut communities have been studied with 16S rRNA clone libraries or culture based methods and only one pyrosequenced zebrafish gut community study – in which individual samples were pooled - is available[1,2]. Therefore, a detailed microbial community analysis of fish guts using the power of next generation sequencing on individual samples is lacking. Here we analyze pyrosequenced amplicon libraries of the gut communities of 11 Atlantic Cod (Gadus morhua) specimens caught in the outer Oslo Fjord. We find that the most abundant taxa are shared among individuals, an observation supporting the idea of the presence of a core gut microbiome in fish species.
Dealing with heterogeneous data to improve our knowledge of biodiversity dynamics and ecosystem function: perspectives from synthesis projects: presented by Liliana Ballesteros-Meija for ACTIAS (Global patterns of insect diversity, distribution and evolutionary distinctness - What can we learn from two of the best-documented families of moths?) at the sfécologie conference 2018.
more information on the group: http://www.cesab.org/index.php/fr/projets-en-cours/projets-2014/130-actias
"Estimation of Divergence Times in Asparagales in the Presence of Hybridization," presented in symposium "Insights and Benefits from Monocot Palaeobiology: Fossils, DNA, and Phylogenies" at Monocots V (5th International Conference on Comparative Biology of Monocotyledons, The New York Botanical Garden, July 2013).
Los días 7 y 8 de mayo organizamos en la Fundación Ramón Areces con la Fundación General CSIC el Simposio Internacional 'Microbiología: transmisión'. La "transmisión" en microbiología hace referencia al proceso por el que material genético es transferido de una célula a otra, de una población a otra. Es un proceso clave para entender el origen y la evolución de los seres vivos. El objetivo de esta reunión era conocer mejor la logística de la transmisión para ser capaces de modular o suprimir algunos procesos de transmisión dañinos.
Introduzione al fund raising in rete - maggio 2015 v0.1Claudio Tancini
Questo semplice documento serve come introduzione per discutere come organizzare una propria attività di fund raising in rete. Non e' un corso esteso ma introduce alcuni argomenti utili e alcuni punti di attenzione
Dr. Peter Davies - Emerging Issues in Antibiotic Resistance Linked to Use in ...John Blue
Emerging Issues in Antibiotic Resistance Linked to Use in Food Animals - Dr. Peter Davies, College of Veterinary Medicine, University of Minnesota, from the 2017 Allen D. Leman Swine Conference, September 16-19, 2017, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2017-leman-swine-conference-material
We investigated the effects of fish protein hydrolysate (FPH) on zootechnical performance and immune response of the Asian Seabass Lates calcarifer Bloch. Experimental fish were fed with 3 diets: a local commercial diet (control), coated or not, with 2 and 3% FPH (w/w). Twelve thousand Asian Seabass juveniles (5.88±0.56 g) were divided into three groups and two replicates reared in nursery tanks (2000 L). The remaining fish were then used for grow-out experiment in floating net cages (1m x 1 m x 3 m). Zootechnical performances were assessed at both stages with following indicators: total weight gain (TWG), % relative weight gain (% RWG), % specific growth rate (% SGR), final weight (g) and final length (cm). At the end of each trial period, fish immune status was assessed through blood sampling and the measurement of Neutrophile (%), Monocyte (%), Lymphocyte (%), Macrophage (105 cell/mL), Leukocyte (103 cell/mL) and Phagocytes activity (%). At the end of the nursery trial, an immersion bacterial challenge with Vibrio parahaemolyticus (105 cells mL-1) was implemented. The results showed that dietary FPH supplementation significantly influenced the growth and immune status of Asian Seabass when compared to the control group. Fish fed FPH supplemented diet yielded higher growth rates and survival rates than non supplemented group. Fish phagocytic activity and resistance to a bacterial challenge were also improved by dietary FPH supplementation. These results may be related to the significant changes observed in fish leukocyte profiles, when fed FPH supplemented diets. Altogether, these results show the positive contribution of FPH to the sustainability of Asian seabass farming.
Prevalence and Antibiotic sensitivity pattern of Salmonella isolates from mil...IOSRJAVS
The study was conducted to determine the presence of Salmonella in retail milk products and water reservoirs in Maiduguri, Nigeria. A total of 150 samples were collected using convenient sampling technique. One hundred samples; fifty each of full cream milk or Kindirmo (n=50) and Skimmed milk or Nono (n=50) were collected from Bulumkutu and Monday market retail sellers. Furthermore, of the fifty samples obtained from the two different locations, twenty five samples each of kindirmo and nono were collected. The samples were aseptically kept in sterile plastic bags. Additionally, fifty water samples were collected from reservoirs within the University campus. They were processed according to standard bacteriological protocols followed by Gram’s staining and biochemical test; Triple sugar iron test, citrate and urease. The Salmonella isolates were further subjected to ten different antibiotics to determine their sensitivity. The overall prevalence of Salmonella in milk samples was found to be 10.00%, while the total prevalence of Salmonella from water sample was 40.00%. Of the fifteen isolates tested, resistance to Amoxicillin, Ceftriaxone and Erythromycin was 100.00%, Gentamicin had 80.00% and Cotrimoxazole was 53.33%, whereas Gentamycin, Ceftriaxone and Amoxicillin displayed 100.00% sensitivity. Moderate sensitivity of 53.33% to Streptomycin and Pefloxacin, 46.67% to Ciprofloxacin and 86.67% sensitivity to Ofloxacin was found in this study. Antibiotic resistance is associated with frequent usage both in livestock and humans as they are commonly available. Adequate sanitary measures should be ensured in milk processing and use of water reservoirs. Antibiotics should be used based on their antibiogram pattern. Prudent use of antibiotics is essential and its continuous use as growth promoters should be discouraged, as this may result to failure in the treatment of Salmonella-associated diseases due to resistance. Disease surveillance programmes should be established as a means for curtailing salmonellosis.
Effect of Stocking Density on the Resistance to Fasting, Growth and Survival ...Agriculture Journal IJOEAR
Abstract— After artificial reproduction of African catfish Heterobranchus bidorsalis, larvae of two days old and 2.18 ± 0.35 mg of mean weight were used to perform two experiments in order to assess the effect of stocking density on their fasting resistance, growth and survival. During the first experiment which lasted 11 days with four batches of larvae at densities of 1, 2, 3 and 4 individuals/ml, results showed that density did not significantly affect (p˂0,05) the resistance to fasting of larvae. However, first mortalities were observed at D5 for all the densities, the higher daily mortality was recorded at D10 and the last mortalities were obtained at D12.
Results of the second experiment revealed that the weight and growth performance of larvae decreased with the increasing of the density after 28 days of rearing. In contrast, the larval survival rate increased with the density. The values of survival rate were respectively 30.53 ± 4.32 and 55.30 ± 21.70 % for the densities 1 ind./l and 20 ind./l.
Few species are standardized and have been used as test organisms around the world in
ecotoxicological assays. In the case of sediment assessment, there are only two amphipod species
(Tiburonella viscana and Grandidierella bonnieroides) standardized protocols for toxicity test in South
Atlantic region.
Few species are standardized and have been used as test organisms around the world in
ecotoxicological assays. In the case of sediment assessment, there are only two amphipod species
(Tiburonella viscana and Grandidierella bonnieroides) standardized protocols for toxicity test in South
Atlantic region
Risk assessment for Listeria monocytogenes in hot-smoked fish in informal mar...ILRI
Presentation by K. Bomfeh, K. Tano-Debrah, F.K. Saalia and B. Bediako-Amoa at the 17th Faculty of Science Colloquium, University of Ghana, Accra, Ghana, 21 March 2012.
Similar to POMS the latest R&D Results - Kube et al (20)
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
1. POMS – The Latest R&D Results
Shellfish Futures, Adventure Bay, Tasmania. 2 October 2013
2. Pacific Oyster Mortality Syndrome POMS
Ostreid herpesvirus (OsHV-1uvar)
France 2007-8
New Zealand 2010
Georges River 2010
Sydney Harbour 2011
Hawkesbury River 2013
3. Mooney
Marra
Mullet Ck
Coba
Porto
DAY 0:
DAY 1:
DAY 3:
DAY 8:
First sighting (30% mortality on one lease)
By afternoon mass mortality
10 million dead oysters
Entire system affected
4. The R&D Response
1. Genetic selection for resistance
2. Develop a laboratory infection model system
3. Epidemiology and husbandry practices to
reduce economic losses
5. Three things you need to know
about breeding for resistance to
Pacific oyster mortality syndrome
Peter Kube (CSIRO)
Mike Dove (NSW DPI)
Matthew Cunningham (ASI)
Paul Hick (NSW DPI)
Wayne O’Connor (NSW DPI)
Peter Kirkland (NSW DPI)
Nick Elliott (CSIRO)
6. POINT #1:
There is good genetic variation
for POMS resistance
in our population
25. A laboratory model for infection
of Pacific oysters with Ostried
herpesvirus type-1 (OsHV-1)
Paul Hick, Mike Dove, Wayne O’Connor,
Xingnian Gu, Andrew Read, Peter Kirkland
27. GOAL: Develop procedures to infect oysters with OsHV-1
• OsHV-1 has been isolated from
oyster tissues (original Georges R
outbreak)
• Used to infect disease free oysters
• Developed procedures for long
term storage (including cryopreservation)
• Ongoing use of the same, well
characterised isolate is possible
28. Successful infection of spat by immersion
•
•
•
Adding virus to water was sufficient to infect and kill spat
(effective in 3 separate trials including with cryopreserved OsHV-1)
Variable results for juveniles and work is ongoing
Work has started on a challenge for larvae
Immersion challenges preferred because:
•
closer to natural infection compared to injection
•
Only practical way of managing large numbers of individuals
29. Ongoing work:
– Determine long-term stability of cryopreserved OsHV-1
– Define difference in susceptibility with age more closely
– Assess repeatability of laboratory infection model
– Apply laboratory challenge in genetic resistance project
30. Husbandry practices to reduce OsHV-1
mortality of Pacific oysters Crassostrea gigas
First steps towards integrated management
within an infected estuary
Ika Paul-Pont, Olivia Evans, Navneet Dhand, Ana
Rubio, Richard Whittington
Faculty of Veterinary Science – The University of Sydney
31. Field trials – Georges River
Height experiment in trays: summers 2011/2012
2012/2013
Standard
growing height
Better survival?
Immersion time
Exposure to virus
Higher
growing height
+300mm
Woolooware Bay, Georges River, NSW
> Triploid PO 11-12 month old:
Reduction of mortality by 25-50%
Maximum mortality at high height : 30-50%
> Triploid PO 2-7 month old:
No significant reduction of mortality
Low
Cumulative mortality (%)
Results:
50%
High
32. Window of infection – Georges and Hawkesbury Rivers
Every two weeks:
12 000 spat deployed at 8 sites
Spat collected, replaced by a new batch
Growth/mortality rates + PCR testing for OsHV-1 (n=30-100 spat per site)
Botany Bay - Georges River
2011/2012: November – May
2012/2013: November – May
Site A
Broken Bay - Hawkesbury River
2012/2013: January – May
Marra Marra
Creek
Mullet Creek
Site B
Site C
Patonga
Kimmerikong
Porto Bay
33. Safe spat experiment - 2013
March 2013: Land based experiment installed on-shore adjacent to the Hawkesbury River
Development of safe rearing techniques (farmers and hatcheries)
Specific water treatments to prevent disease occurrence
Co-funding: Tasmanian Oyster Research Committee
n=2000 spat/treatment
Flow rate= 5L/min/tank
No food supply
Daily sampling / mortality check
34. Safe spat experiment - 2013
Mortality
100%
Control (107 –109 viral DNA copies/g)
Sampling collection (n=40 per treatment)
and PCR testing
Cumulative mortality (%)
River (104 –108 viral DNA copies/g)
80%
60%
Chiller (107 –108 viral DNA copies/g)
40%
Sedimentation
20%
No virus detected
UV+filtration
No virus detected
0%
6-Apr
10-Apr
14-Apr
18-Apr
22-Apr Date
26-Apr
30-Apr
4-May
8-May
12-May
Conclusions:
- water treatments and land based system allowed spat survival in an infected estuary
- the role of UV and filtration remains unclear and need to be tested separately
- the 48h sedimentation provided the best outcome (cheap, easy) but needs to be confirmed
35. What can R&D deliver
in the medium term?
1. A medium term solution is expected from a combination of
resistant stock and changed husbandry practices
2. A laboratory infectivity model will be available and will be an
essential tool for breeding, and other, work
3. Useful levels of resistance are expected in juveniles by mid
2016 (three more years of selection)
4. Given the time required for commercial scale-up, this stock
won’t be available until 2018 at the earliest (as diploid)
36. Project Staff and Acknowledgements
NSW DPI
Mike Dove
Paul Hick
Peter Kirkland
Wayne O’Connor
Xingnian Gu
Andrew Read
FUNDING ASSISTANCE
Oysters Australia
Seafood CRC
FRDC
CSIRO
Peter Kube
Nick Elliott
ASI
Matthew Cunningham
University of Sydney
Ika Paul-Pont
Olivia Evans
Navneet Dhand
Ana Rubio
Richard Whittington
FIELD SITE (Georges River)
Drakes Oysters
