Instrumentation of mass spectrometery
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Mass spectrometry is an instrumental technique that analyzes samples by converting molecules to ions and separating them based on their mass-to-charge ratios. It works by first ionizing a sample through electron bombardment, then accelerating the ions and passing them through electric and magnetic fields to deflect them into a detector based on their masses. The detector then records the number of ions at each mass, allowing the user to determine the composition of the original sample.
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Instrumentation of mass spectrometery
- 2. • Mass spectrometery is an instrumental technique in which sample is converted to rapidly moving positive ions by electron bombardment and charge particales are separated according to their masses
- 4. • 1 Sample Inlet • 2 Ionization • 3 Accelration • 4 Deflection • 5 Detection
- 5. • Sample stored in a large reservoir from which molecule reaches the ionization chamber at low pressure in the steady stream by by a pinhole called “Molecular Leak”
- 6. • The atom is ionised by knocking one or more electrons off to give a positve ion.(Mass spectrometer work with always positve ions) • Most of the positve ions formed will carry a charge of +1
- 7. • The particals in the sample (atom or molecule) are bombarded with a streem of electrons to knock one or more electrons out of the sample particals to make positve ions
- 8. The positve ions are persuaded out in to the rest of the machine by the ions repeller whichis an other metal plate carrying a slight positve charge. Ionization can be achieved by § Electron Ionization § chemical ionization § Desorption ionization
- 9. • The ions are accelrated so that they have the same the kinetic energy
- 10. • The positve ios are repelled away from the positve ionization chamber and pass through three slit with voltage in the decreasing order • The middle slit carries some intermediate voltage and the final at “ 0” volt • All the ions are accelrated into a finely focused beam
- 11. • The ions are then deflected by a magnatic field according to their masses.The lighter they are the more it get are deflected • The amount of deflection also depend on the number of positve charges on the ion.The more the ions is charged the more it gets deflected
- 13. • Different ions are deflected by the magnatic field by different amounts.The amount of deflection depends upon.
- 14. • The beam of ions passing through the machine is detected electrically
- 16. • When an ion hits the metal box its charge is neutralised by an electron jumping from the metal on to the ion • That leaves a space amongst the electrons in the metal and the electrons in the wire shuffle along to fill it
- 17. • A flow of electrons in the wire is detected as an electric current which can be amplified and recorded .The more ions arriving the greater the current