This document provides instructions for streaking culture plates in bacteriology. It explains that streaking involves placing a small sample on an agar plate and using a sterile loop to spread it out in lines, transferring a small amount with each streak to isolate single bacterial cells. The goal is to obtain separated pure colonies when the plate is incubated. It emphasizes the importance of sterilizing the loop before and after each use and working aseptically to prevent contamination. The detailed method described includes inoculating the loop, streaking in perpendicular lines while turning the plate, and sealing and incubating the finished streaked plate.
COLLECTION AND TRANSPORTATION OF CLINICAL SAMPLESNCRIMS, Meerut
Principles of Sample Collection:
Aseptic precautions to minimize chances of
contamination.
Appropriate anatomic sites
Adequate volume
Adequate no. of samples
Appropriate time
Appropriate container with proper labelling
Before initiation of anti-microbials
Adequate information in request form
Microbial cultures are foundational and basic diagnostic methods used extensively as a research tool in molecular biology.
Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both.
It is one of the primary diagnostic methods of microbiology and used as a tool to determine the cause of infectious disease by letting the agent multiply in a predetermined medium.
It is often essential to isolate a pure culture of microorganisms
COLLECTION AND TRANSPORTATION OF CLINICAL SAMPLESNCRIMS, Meerut
Principles of Sample Collection:
Aseptic precautions to minimize chances of
contamination.
Appropriate anatomic sites
Adequate volume
Adequate no. of samples
Appropriate time
Appropriate container with proper labelling
Before initiation of anti-microbials
Adequate information in request form
Microbial cultures are foundational and basic diagnostic methods used extensively as a research tool in molecular biology.
Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both.
It is one of the primary diagnostic methods of microbiology and used as a tool to determine the cause of infectious disease by letting the agent multiply in a predetermined medium.
It is often essential to isolate a pure culture of microorganisms
Antimicrobial sensitivity testing (AST) or Antibiotic Sensitivity Testing.
Contents:
1. Need of AST
2. Bacterial Resistance
3. Preperation of test: selection of antibiotic and bacteria
4. Types of tests
5. Process of tests
The use of a machine designed to follow repeatedly and automatically a predetermined sequence of individual operations.
AUTOMATED WASHING
AUTOMATED MEDIA PREPARATORS
AUTOMATED COLLECTION AND
PROCESSING OF SAMPLES
CYTOSPIN
AUTOMATED GRAM STAINING
AUTOMATED STREAKING
SPIRAL PLATER
AUTOMATED ANTIBIOTIC -
SENSITIVITY SYSTEM
AUTOMATIC COLONY COUNTER
AUTOMATED URINE MICROSCOPY -
ANALYSER
Antimicrobial sensitivity testing (AST) or Antibiotic Sensitivity Testing.
Contents:
1. Need of AST
2. Bacterial Resistance
3. Preperation of test: selection of antibiotic and bacteria
4. Types of tests
5. Process of tests
The use of a machine designed to follow repeatedly and automatically a predetermined sequence of individual operations.
AUTOMATED WASHING
AUTOMATED MEDIA PREPARATORS
AUTOMATED COLLECTION AND
PROCESSING OF SAMPLES
CYTOSPIN
AUTOMATED GRAM STAINING
AUTOMATED STREAKING
SPIRAL PLATER
AUTOMATED ANTIBIOTIC -
SENSITIVITY SYSTEM
AUTOMATIC COLONY COUNTER
AUTOMATED URINE MICROSCOPY -
ANALYSER
flu sample collection poster from CDC guideliness .A common viral infection that can be deadly, especially in high-risk groups.
The flu attacks the lungs, nose and throat. Young children, older adults, pregnant women and people with chronic disease or weak immune systems are at high risk.
This presentation describes how to give vaccinations and subcutaneous fluids to animals. It has been designed for an animal shelter, humane society, or rescue setting.
http://abcr.com Everything you need to know about environmental sampling or food preparation surfaces, countertops, carcasses, and more from prep to shipping. Watch the YouTube video at the end of the presentation!
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
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An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
2. Think Before Inoculation
2
• Before inoculation,
important information is
written on the bottom of
the plates, close to the rim
• 1date of inoculation
• 2.temperature of incubation
• 3.duration of incubation
• 4.microorganism inoculated
3. What is streaking
3
• The most common method of inoculating an agar plate is streaking.
•Streak plates
• 1.With this method, a small amount of sample is placed on the side of the agar
plate (either with a swab, or as a drop from an inoculating loop if the sample is
a liquid).
• 2.A sterile loop (flamed until red hot, then cooled by touching the agar away
from the inoculated sample) is then used to spread the bacteria out in one
direction from the initial site of inoculation.This is done by moving the loop
from side to side, passing through the initial site
4. What is streaking
4
•The loop is then sterilised (by flaming) again and the
first streaks are then spread out themselves.
•.This is repeated 2-3 times, moving around the agar
plate.
•What should happen is that single bacterial cells get
isolated by the streaking, and when the plate is
incubated, forming discrete colonies that will have
started from just one bacterium each.
5. The essential step in inoculating
culture plates
5
•There are several
essential precautions that
must be taken during
inoculation procedures to
control the opportunities
for the contamination of
cultures, people or the
environment
6. Prompt action with Optimal
utility
6
•Operations must
not be started until
all requirements
are within
immediate reach
and must be
completed as
quickly as possible
7. Expose the inoculum in test tubes and
containers for minimal Time
7
• Inoculum is grown in test
tubes and must be open for
the minimum amount of
time possible and while
they are open all work must
be done close to the Bunsen
burner flame where air
currents are drawn
upwards.
8. The Neck of Test tube containing
inoculum to be heated briefly
8
•On being opened, the
neck of a test tube or
bottle must be
immediately warmed by
flaming so that any air
movement is outwards
and the vessel held as
near as possible to the
horizontal
9. Exposure to Environment should limited to
minimum
9
•During manipulations
involving a Petri dish,
exposure of the sterile
inner surfaces to
contamination from
the air must be limited
to the absolute
minimum
10. Work with absolute sterility
10
• The parts of sterile pipettes
that will be put into cultures or
sterile vessels must not be
touched or allowed to come in
contact with other non-sterile
surfaces, e.g. clothing, the
surface of the working area,
the outside of test
tubes/bottles
11. Work Using a wire loop
11
• Wire loops are sterilised using red
heat in a Bunsen flame before and
after use.They must be heated to red
hot to make sure that any
contaminating bacterial spores are
destroyed.The handle of the wire loop
is held close to the top, as you would a
pen, at an angle that is almost
vertical.This leaves the little finger
free to take hold of the cotton wool
plug/screw cap of a test tube/bottle
12. Flaming procedure
12
• The flaming procedure is
designed to heat the end of
the loop gradually because
after use it will contain
culture, which may
‘splutter’ on rapid heating
with the possibility of
releasing small particles of
culture and aerosol
formation
13. Handling of the Inoculating loop
13
•Position the
handle end of the
wire in the light
blue cone of the
flame.This is the
cool area of the
flame
14. Perfect the art handling the Inoculating
loop
14
•Draw the rest of the
wire upwards slowly up
into the hottest region
of the flame,
(immediately above
the light blue cone). .
Hold there until it is
red hot.
15. Heat and cool the Loop
15
• Ensure the full
length of the wire
receives
adequate
.
heating.
Allow to cool
then use
immediately.
16. Handle the Inoculating loop
16
Do not put the
loop down or wave
it around..
Re-sterilise the
loop immediately
after use.
17. Be careful some times the Loop may not contain
inoculum must redraw the inoculum
17
• If a loop does not hold any
liquid the loop has not made a
complete circle.To correct the
problem, first ensure that the
loop has been sterilised and
then reshape the loop with
forceps. Do not use your
fingers because of the
possibility of puncturing the
skin.
18. Working with bacteria and yeast Streak plate
•The loop is used for
preparing a streak plate.
This involves the
progressive dilution of an
inoculum of bacteria or
yeast over the surface of
solidified agar medium in a
Petri dish in such a way that
colonies grow well
se5
p
/7/2
a
01r
6 ated from each other.
20. The aim of the procedure is to obtain
single isolated pure colonies
• Loosen the cap of the bottle containing the inoculum.
• Hold the loop in the
.
right hand.
Flame the loop and
allow to cool.
20
22. Handling matters in getting the right
inoculum
Lift the bottle/test tube
containing the inoculum
with the left hand. .
Remove the cap/cotton
wool plug of the bottle/test
tube with the little finger of
the right hand. .
Flame the neck of the
bottle/test tube.
22
23. Handling loop and Inoculating the material
matters
•Insert the loop into
the culture broth
and withdraw. At
all times, hold the
loop as still as
possible. .
Flame neck of the
bottle/test tube.
23
25. Practice for
Perfection
• Replace the cap/cotton
wool plug on the
bottle/test tube using
the little finger. Place
bottle/test tube on
bench. . Partially lift the
lid of the Petri dish
containing the solid
medium.
25
26. Practice , Practice best way to Perfection
• Hold the charged loop
parallel with the surface of
the agar; smear the
inoculum backwards and
forwards across a small
area of the medium (see
streaked area .
26
27. Follow the Instructions for safe keeping of
Petri dish
• Remove the loop and
close the Petri dish.
. Flame the loop and
allow it to cool.
Turn the dish through
90° anticlockwise.
27
28. Streak the Plates in a defined Manner
•With the cooled loop
streak the plate from
area ‘ across the
surface of the agar in
three or four parallel
lines . Make sure that
a small amount of
culture is carried over.
28
29. Streak the Plates in a defined Manner
• Remove the loop and
close the Petri dish. .
Flame the loop and
allow to cool.Turn the
dish through 90°
anticlockwise again and
streak from ’ across the
surface of the agar in
three or four parallel
lines .
29
30. Carry with further inoculations
•Remove the loop and close
the Petri dish. Flame the
loop and allow to cool.Turn
the dish through
90°anticlockwise and streak
loop across the surface of the
agar from ‘ into the centre of
the plate
30
31. Complete the work Close the petri dish
• Remove the loop and
close the Petri dish.
Flame the loop.
. Seal and incubate the
plate in an inverted
position.There are
alternative methods for
preparing a streak.
31