www.biolifejournal.com.
Biolife is an open access, online, peer reviewed international journal with a primary objective to provide research and applications related to all the Biology and Life Sciences
Evaluation of Anti-oxidant Activity of Elytraria acaulis Aerial ExtractsIJERA Editor
Elytraria acaulis, a stem less perennial herb of Acantheceae family has many medicinal and therapeutic properties. Anti oxidative activity of the aerial parts of this Elytraria acaulis were assessed in the present study. The aerial parts of the plant (Stem & Leaves) were extracted in different organic solvents such as n-Hexane, Ethanol, Methanol, Ethyl Acetate and Chloroform. Initially, Total Phenolic & Total Flavonoids content in different solvent plant extracts were estimated. The free radical scavenging and antioxidant activity of the Elytraria acaulis aerial extracts in different organic solvents were also assayed by DPPH assay, FRAP assay. The aerial extracts of Elytraria acaulis have shown significant anti oxidant activity. Hence, further studies on this plant will enable elucidation of its therapeutic properties and medicinal applications
Applying new techniques to blood assays in cats has enabled researchers to reduce the amount of blood needed nutrition study sample collection studies by 80%, with concomitant benefits for animal welfare. Presented at the Waltham InternationaI Nutrition Science Symposium, October 2016, Chicago
Anti neoplastic effect of Eclipta prostrata L. (HepG2) cell lines. Hepatocellular carcinoma (HCC) is a tumor of the liver. HCC is responsible for over 12,000 deaths per year in the United States. It is one of the serious health problems in most developing countries. The present probe proved that ethanol extract of Eclipta prostrata L. significantly suppressed the growth and induced the apoptosis in the liver cancer (HepG2) cell lines. IC50 dose was measured with methyl thiazolyl tetrazolium. 100 μg of extract showed 50% reduction of in HepG2 cell line growth at 48 h of incubation. The whole plant of E. prostrata L. extract-induced apoptotic features of cell death was stained with acridine orange. The intracellular enzymatic antioxidants such as superoxide dismutase, glutathione peroxidase, and catalase were slightly decreased in their activities when compared to control. Thus, the study resolves that E. prostrata L. extract is an effective to prevent or retard the spread of malignant cells and antineoplastic effect.
www.biolifejournal.com.
Biolife is an open access, online, peer reviewed international journal with a primary objective to provide research and applications related to all the Biology and Life Sciences
Evaluation of Anti-oxidant Activity of Elytraria acaulis Aerial ExtractsIJERA Editor
Elytraria acaulis, a stem less perennial herb of Acantheceae family has many medicinal and therapeutic properties. Anti oxidative activity of the aerial parts of this Elytraria acaulis were assessed in the present study. The aerial parts of the plant (Stem & Leaves) were extracted in different organic solvents such as n-Hexane, Ethanol, Methanol, Ethyl Acetate and Chloroform. Initially, Total Phenolic & Total Flavonoids content in different solvent plant extracts were estimated. The free radical scavenging and antioxidant activity of the Elytraria acaulis aerial extracts in different organic solvents were also assayed by DPPH assay, FRAP assay. The aerial extracts of Elytraria acaulis have shown significant anti oxidant activity. Hence, further studies on this plant will enable elucidation of its therapeutic properties and medicinal applications
Applying new techniques to blood assays in cats has enabled researchers to reduce the amount of blood needed nutrition study sample collection studies by 80%, with concomitant benefits for animal welfare. Presented at the Waltham InternationaI Nutrition Science Symposium, October 2016, Chicago
Anti neoplastic effect of Eclipta prostrata L. (HepG2) cell lines. Hepatocellular carcinoma (HCC) is a tumor of the liver. HCC is responsible for over 12,000 deaths per year in the United States. It is one of the serious health problems in most developing countries. The present probe proved that ethanol extract of Eclipta prostrata L. significantly suppressed the growth and induced the apoptosis in the liver cancer (HepG2) cell lines. IC50 dose was measured with methyl thiazolyl tetrazolium. 100 μg of extract showed 50% reduction of in HepG2 cell line growth at 48 h of incubation. The whole plant of E. prostrata L. extract-induced apoptotic features of cell death was stained with acridine orange. The intracellular enzymatic antioxidants such as superoxide dismutase, glutathione peroxidase, and catalase were slightly decreased in their activities when compared to control. Thus, the study resolves that E. prostrata L. extract is an effective to prevent or retard the spread of malignant cells and antineoplastic effect.
The efficacy of Lemon (Citrus lemonirisso) juice on wound healing of albino wistar rat was investigated; also the potential haemostatic mechanism associated with administration of the extract was investigated. Results showed that lemon juice extract decreased haemoglobin concentration, packed cell volume while it has no significant effect on platelet count, white blood cell count and white cell differential counts in albino rats. Furthermore, the bleeding and clotting times were shortened and the period of healing of wound using lemon juice could possess some elements that is affecting the haemostatic mechanism.
Enzymatic Saccharification of Lignocellulosic BiomassBiorefineryEPC™
Enzymatic Saccharification of Lignocellulosic Biomass
YOU AGREE TO INDEMNIFY BiorefineryEPCTM , AND ITS AFFILIATES, OFFICERS, AGENTS, AND EMPLOYEES AGAINST ANY CLAIM OR DEMAND, INCLUDING REASONABLE ATTORNEYS' FEES, RELATED TO YOUR USE, RELIANCE, OR ADOPTION OF THE DATA FOR ANY PURPOSE WHATSOEVER. THE DATA ARE PROVIDED BY BiorefineryEPCTM "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE EXPRESSLY DISCLAIMED. IN NO EVENT SHALL BiorefineryEPCTM BE LIABLE FOR ANY SPECIAL, INDIRECT OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER, INCLUDING BUT NOT LIMITED TO CLAIMS ASSOCIATED WITH THE LOSS OF DATA OR PROFITS, WHICH MAY RESULT FROM ANY ACTION IN CONTRACT, NEGLIGENCE OR OTHER TORTIOUS CLAIM THAT ARISES OUT OF OR IN CONNECTION WITH THE USE OR PERFORMANCE OF THE DATA.
This study was designed to evaluate cytotoxic of
70% ethanolic crude extract of Portulaca oleracea L on normal
human lymphocytes (in vitro) In vitro two, parameters were
conducted; mitotic index (MI) and blast index (BI) .The cytotoxic
effect of plant extract of Portulaca oleracea at (6.4, 3.2, 1.6, 0.8,
0.4 and 0.2 mg/ml) on normal human peripheral lymphocytes in
vitro, for 72 hrs was studied. Results indicated a positive
relationship between Mitotic index (MI) values and concentration
of ethanolic plant extract (0.467±0.03, 0.467±0.09, 0.300±0.06,
0.267±0.07, 0.300±0.07 and 0.267±0.07) respectively, no
significant differences noted (P≥0.01) among effects of different
concentrations. Mitotic index value of positive control treatment
(PHA) (0.700±0.12) differed significantly (P≤0.01) with all
concentrations. Blast index (BI) values following treatment with
different concentration of ethanolic extract of Portulaca oleracea
(6.4, 3.2, 1.6, 0.8, 0.4 and 0.2 mg/ ml) were (4.03±0.37,
3.70±0.17, 3.30±0.61, 3.13±0.24 and 2.77±0.20), without
significant differences (P≥0.01).Following treatment with the
highest concentration of plant extract (6.4 mg/ml), the BI value
was (5.37±0.30) significantly higher (P≤0.01) than those
following treatment with other concentrations. Positive control
treatment showed highly significant elevation (P≤0.01) in BI
(7.67±0.56) compared with all treatments.
Improved coverage of the proteome using gel eluted liquidExpedeon
It has long been understood that sample fractionation is critically important to generating quality, comprehensive proteomics data. In spite of the continual improvements in speed and sensitivity of mass spectrometers, these instruments are still unable to adequately overcome the enormous challenge
of most biological samples without multiple dimensions of separation prior to mass analysis.
Characterization of intact antibodies by pre-fractionation using gel electrop...Expedeon
Antibodies represent an important class of proteins due to their central role in the immune response. Moreover, there is an increasing interest in the use of recombinant antibodies as novel drug therapies.
TOTAL POLYPHENOLS AND DPPH FREE RADICALS SCAVENGING ACTIVITY IN SIX LEAFY VEG...Md. Kamaruzzaman
TOTAL POLYPHENOLS AND DPPH FREE RADICALS SCAVENGING ACTIVITY IN SIX LEAFY VEGETABLES OF BANGLADESH
Harun-Ar-Rashid, Sheikh Julfikar Hossain, Sk. Amir Hossain, Md Mahfuzur Rahman, Md. Kamaruzzaman
This slide is about grape seed extract and their structures, along with the extraction methods of grape seed oils. explains about the uses and side effects.Their antioxidant property.
Investigate and Compare Chemical Compositions in Ginkgo biloba ProductsCassandra Quave
Undergraduate student research student from Quave Lab. Poster presentation given April 2016.
Ginkgo, sometimes called maidenhair tree, is the only living member of the botanical family Ginkgoaceae.
Medicinal usage was recorded in Chinese Materia Medica Ben cao gang mu by Li Shizhen in 16th Century.
Historical record: Most historical reference to the medicinal use of ginkgo refers to the seed. For example, in Ben Cao Gang Mu, ginkgo seed was cited for its use in the treatment of “ asthma, coughs, irritability of the bladder, blennorrhea, and uterine fluxes” (Read 1982).
Modern usage: the highly concentrated extract of ginkgo leaf has been primarily used to enhance mental acuity and promote peripheral circulation in dementia such as Alzheimer’s disease.
External usage: effective treatment for infectious skin diseases. A specifically characterized ginkgo leaf extract contains 24% flavonol glycosides; 6% terpene trilactones; < 5ppm ginkgolic acids.
Combination of Angkak (Red Yeast Rice), Red Guava (Psidium guajava Linn) Leaf...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Identifying Active Compounds Extracted from Hypericum perforatum to Character...Cassandra Quave
This is a poster presentation given by an undergraduate researcher in the Quave Lab in April 2016.
Hypericum perforatum (St. John’s wort) is a well known medicinal herb often associated with the treatment of anxiety and depression. However, an oleolite preparation of the flowers is also widely used in traditional medicine across Eastern Europe and the Balkans. Recent research has shown that this oleolite reduces both wound size and healing time. H. perforatum has been well characterized chemically. Many secondary metabolites have been identified including: naphthodianthrones (hypericin), phloroglucinols (hyperforin), flavonoid glycosides (hyperoside), biflavones and anthocyanidins. The phloroglucinol hyperforin and its derivatives have also been reported as being responsible for its antibacterial activity.8 However, phloroglucinols are quite unstable with light and heat, and thus should not be present in the aged oleolite preparation of H. perforatum. Additionally, hypericin can cause phototoxic skin reactions if ingested or absorbed into the skin, as evidenced by livestock that develop extreme photosensitivity after grazing on H. perforatum flowers.4 Therefore, the established chemistry presents an interesting paradox to the traditional preparation of H. perforatum. The hyperforin responsible for the antibacterial bioactivty should degrade in the sunlight as the traditional oleolite is prepared. Alternately, if hypericin is present in established bioactive levels, then the traditionally prepared oleolite should cause photosensitivity, yet none is reported. In this research, an organic and aqueous laboratory extract of H. perforatum were compared to a traditional oleolite to better understand the chemical composition of this remedy.
The efficacy of Lemon (Citrus lemonirisso) juice on wound healing of albino wistar rat was investigated; also the potential haemostatic mechanism associated with administration of the extract was investigated. Results showed that lemon juice extract decreased haemoglobin concentration, packed cell volume while it has no significant effect on platelet count, white blood cell count and white cell differential counts in albino rats. Furthermore, the bleeding and clotting times were shortened and the period of healing of wound using lemon juice could possess some elements that is affecting the haemostatic mechanism.
Enzymatic Saccharification of Lignocellulosic BiomassBiorefineryEPC™
Enzymatic Saccharification of Lignocellulosic Biomass
YOU AGREE TO INDEMNIFY BiorefineryEPCTM , AND ITS AFFILIATES, OFFICERS, AGENTS, AND EMPLOYEES AGAINST ANY CLAIM OR DEMAND, INCLUDING REASONABLE ATTORNEYS' FEES, RELATED TO YOUR USE, RELIANCE, OR ADOPTION OF THE DATA FOR ANY PURPOSE WHATSOEVER. THE DATA ARE PROVIDED BY BiorefineryEPCTM "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE EXPRESSLY DISCLAIMED. IN NO EVENT SHALL BiorefineryEPCTM BE LIABLE FOR ANY SPECIAL, INDIRECT OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER, INCLUDING BUT NOT LIMITED TO CLAIMS ASSOCIATED WITH THE LOSS OF DATA OR PROFITS, WHICH MAY RESULT FROM ANY ACTION IN CONTRACT, NEGLIGENCE OR OTHER TORTIOUS CLAIM THAT ARISES OUT OF OR IN CONNECTION WITH THE USE OR PERFORMANCE OF THE DATA.
This study was designed to evaluate cytotoxic of
70% ethanolic crude extract of Portulaca oleracea L on normal
human lymphocytes (in vitro) In vitro two, parameters were
conducted; mitotic index (MI) and blast index (BI) .The cytotoxic
effect of plant extract of Portulaca oleracea at (6.4, 3.2, 1.6, 0.8,
0.4 and 0.2 mg/ml) on normal human peripheral lymphocytes in
vitro, for 72 hrs was studied. Results indicated a positive
relationship between Mitotic index (MI) values and concentration
of ethanolic plant extract (0.467±0.03, 0.467±0.09, 0.300±0.06,
0.267±0.07, 0.300±0.07 and 0.267±0.07) respectively, no
significant differences noted (P≥0.01) among effects of different
concentrations. Mitotic index value of positive control treatment
(PHA) (0.700±0.12) differed significantly (P≤0.01) with all
concentrations. Blast index (BI) values following treatment with
different concentration of ethanolic extract of Portulaca oleracea
(6.4, 3.2, 1.6, 0.8, 0.4 and 0.2 mg/ ml) were (4.03±0.37,
3.70±0.17, 3.30±0.61, 3.13±0.24 and 2.77±0.20), without
significant differences (P≥0.01).Following treatment with the
highest concentration of plant extract (6.4 mg/ml), the BI value
was (5.37±0.30) significantly higher (P≤0.01) than those
following treatment with other concentrations. Positive control
treatment showed highly significant elevation (P≤0.01) in BI
(7.67±0.56) compared with all treatments.
Improved coverage of the proteome using gel eluted liquidExpedeon
It has long been understood that sample fractionation is critically important to generating quality, comprehensive proteomics data. In spite of the continual improvements in speed and sensitivity of mass spectrometers, these instruments are still unable to adequately overcome the enormous challenge
of most biological samples without multiple dimensions of separation prior to mass analysis.
Characterization of intact antibodies by pre-fractionation using gel electrop...Expedeon
Antibodies represent an important class of proteins due to their central role in the immune response. Moreover, there is an increasing interest in the use of recombinant antibodies as novel drug therapies.
TOTAL POLYPHENOLS AND DPPH FREE RADICALS SCAVENGING ACTIVITY IN SIX LEAFY VEG...Md. Kamaruzzaman
TOTAL POLYPHENOLS AND DPPH FREE RADICALS SCAVENGING ACTIVITY IN SIX LEAFY VEGETABLES OF BANGLADESH
Harun-Ar-Rashid, Sheikh Julfikar Hossain, Sk. Amir Hossain, Md Mahfuzur Rahman, Md. Kamaruzzaman
This slide is about grape seed extract and their structures, along with the extraction methods of grape seed oils. explains about the uses and side effects.Their antioxidant property.
Investigate and Compare Chemical Compositions in Ginkgo biloba ProductsCassandra Quave
Undergraduate student research student from Quave Lab. Poster presentation given April 2016.
Ginkgo, sometimes called maidenhair tree, is the only living member of the botanical family Ginkgoaceae.
Medicinal usage was recorded in Chinese Materia Medica Ben cao gang mu by Li Shizhen in 16th Century.
Historical record: Most historical reference to the medicinal use of ginkgo refers to the seed. For example, in Ben Cao Gang Mu, ginkgo seed was cited for its use in the treatment of “ asthma, coughs, irritability of the bladder, blennorrhea, and uterine fluxes” (Read 1982).
Modern usage: the highly concentrated extract of ginkgo leaf has been primarily used to enhance mental acuity and promote peripheral circulation in dementia such as Alzheimer’s disease.
External usage: effective treatment for infectious skin diseases. A specifically characterized ginkgo leaf extract contains 24% flavonol glycosides; 6% terpene trilactones; < 5ppm ginkgolic acids.
Combination of Angkak (Red Yeast Rice), Red Guava (Psidium guajava Linn) Leaf...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Identifying Active Compounds Extracted from Hypericum perforatum to Character...Cassandra Quave
This is a poster presentation given by an undergraduate researcher in the Quave Lab in April 2016.
Hypericum perforatum (St. John’s wort) is a well known medicinal herb often associated with the treatment of anxiety and depression. However, an oleolite preparation of the flowers is also widely used in traditional medicine across Eastern Europe and the Balkans. Recent research has shown that this oleolite reduces both wound size and healing time. H. perforatum has been well characterized chemically. Many secondary metabolites have been identified including: naphthodianthrones (hypericin), phloroglucinols (hyperforin), flavonoid glycosides (hyperoside), biflavones and anthocyanidins. The phloroglucinol hyperforin and its derivatives have also been reported as being responsible for its antibacterial activity.8 However, phloroglucinols are quite unstable with light and heat, and thus should not be present in the aged oleolite preparation of H. perforatum. Additionally, hypericin can cause phototoxic skin reactions if ingested or absorbed into the skin, as evidenced by livestock that develop extreme photosensitivity after grazing on H. perforatum flowers.4 Therefore, the established chemistry presents an interesting paradox to the traditional preparation of H. perforatum. The hyperforin responsible for the antibacterial bioactivty should degrade in the sunlight as the traditional oleolite is prepared. Alternately, if hypericin is present in established bioactive levels, then the traditionally prepared oleolite should cause photosensitivity, yet none is reported. In this research, an organic and aqueous laboratory extract of H. perforatum were compared to a traditional oleolite to better understand the chemical composition of this remedy.
More information can be found on:
- www.purepulse.eu
- http://www.pinterest.com/toptechtalks/purepulse-pef-20/
- http://en.topwiki.nl/index.php/PurePulse_-_PEF_2.0
Evaluation of quality changes of blueberry juice during refrigerated storage after high-pressure and pulsed electric fields processing.
More information:
www.cwp-bv.nl
www.purepulse.eu
Poster presentation on the research article carried out during Internship period LEE presented in National Horticulture society Seminar Held in Kirtipur.
EFFECT OF DIFFERENT CHROMATOGRAPHIC FRACTION AQUEOUS AND ALCOHOLIC EXTRACTS O...Jing Zang
In recent studies Teucrium polium(T. polium ) was known as a hypoglycemic plants. But further research is needed to better understand the effect of Teucrium polium and biological active part of it. The purpose of this investigation is to examine the effect of different chromatographic fractions of aqueous and alcoholic extract of this plant on the level of insulin secretion and glucose content in hyperglycemic rat model. Also, our aim is determination of biological active fraction of aqueous and alcoholic extract of this plant. This study was carried out on the 36 rats. Hyperglycemia induced by administrating of 50 mg/kg alloxan intraperitoneally and glucose level was monitored for hyperglycemic status. Hyperglycemic was confirmed by blood glucose measurement. In each experiment 100 grams of Teucrium polium aerial parts powder were boiled with 2 Litter of distilled water for 36 h. The decoction preparation was then filtered through a gauz cloth followed by filtration through filter paper. The extract was evaporated to one-fifth of its original volume and kept at 4oC until its use. Determination of different fraction aqueous extract effect of Teucrium polium on glucose level and insulin secretion was carried out. Blood was collected from the tail of the rats. Then glucose and insulin level was evaluated. The hyperglycemic animals showed significant decrease in the blood glucose level in rats administered with fourth fraction compared with other factions. Administration of fourth fraction Teucrium polium aerial parts extract cause increase in insulin levels in alloxan-treated rats. Results suggest that treatment of fourth fraction Teucrium polium aerial parts extract may be useful in preventing the increase of glucose level in hyperglycemic rats. The interesting phenomenon of our results has shown that fourth fraction given parenterally possesses a hypoglycemic effect in alloxan hyperglycemic rats. Fourth fraction was found biological active and to be responsive to glucose challenge as evidenced by increase in insulin secretion.
ABSTRACT- The invitro anti-inflammatory activity of various solvent fractions of Parkia biglobosa fruit bark was investigated using human red blood cell membrane stabilization, heat-induced hemolysis and protein denaturation methods. All the extracts of P. biglobosa fruit bark showed a concentration dependent increase in anti-inflammatory activity. The anti-inflammatory activity of the crude extract (60.8%, 58.3%, 78.2%) and last remaining aqueous extract (61.1%, 54.1%, 77.2%) have the maximum membrane stabilization, protection against hemolysis and albumin denatura-tion respectively which was comparable to Diclofenac sodium (61.4%, 60.6%, 100%) at 400μg/ml concentration. This study suggests that P. biglobo-sa fruit bark posses enough potential to reduce inflammation, hence directs the importance of further research and development of novel anti-inflammatory agents. Key words- Invitro anti-inflammatory, fruit bark, HRBC membrane stabilization, hemolysis, protection
Comparative Assessment of Total Polyphenols and Antioxidant Activity of Comme...AnuragSingh1049
Green Tea, made from Camellia sinensis plant leaves, is one of the most popular drinks in the world. For the past decades, scientists have studied this plant in terms of potential health benefits. Research has shown that green tea helps prevent stroke, malignancy and infections. In this paper, antioxidant activity and total phenol content of 4 samples of green tea from local Tuzla stores were investigated, of which two were of foreign origin. The antioxidant activity of the samples was analyzed using FRAP and DPPH methods. The obtained results show that the highest content of total phenols and the largest antioxidant capacity has a sample of foreign origin. The content of total phenols in the samples ranges from 60.01 to 79.34 mg GAE/g. The highest FRAP value is 3.34 mmol/g. The antioxidant capacity was also confirmed by the DPPH method. The IC50 value ranges from 0.014 to 0.030 mg/mL.
Total phenolics and total flavonoids of extracts from freshwater Clam (Corbic...Innspub Net
The ethanol, ethyl acetate, and hexane extracts of the freshwater clam (Corbicula fluminea) were studied for the total phenolics and total flavonoids. Total phenolics and total flavonoids of the extracts were evaluated using Folin-Ciocalteau and Aluminum chloride colorimetric methods respectively. The findings showed that the total phenolics of the ethanol extract (1.67±0.28mg GAE/g of dried sample) were substantially higher than the total phenolics obtained from the ethyl acetate (0.70±0.00mg GAE/g) and hexane extracts (0.56±0.23mg GAE/g). While the total flavonoids in the ethyl acetate extract displayed a slightly higher total flavonoid (43.84±0.92mg QE/g of dried sample) relative to ethanol (30.41±1.34mg QE/g of dried sample) and hexane extracts (20.28±0.00mg QE/g of dried sample). Using ethanol, the highest yield for extraction was obtained. Ethanol is the best solvent among the three – ethanol, ethyl acetate, and hexane in terms of extraction yield and total phenolics. In addition, it can be inferred that the presence of significant amounts of phenolics and flavonoids suggests that freshwater clam is a promising source of antioxidants that provides nourishing proteins and oxidative stress remedies
Similar to Impact of PEF and thermal processing on apple juice shelf life (20)
More information can be found on:
- www.purepulse.eu
- http://www.pinterest.com/toptechtalks/purepulse-pef-20/
- http://en.topwiki.nl/index.php/PurePulse_-_PEF_2.0
Lunchlezing op het ministerie van EZ te Den Haag over Disruptieve Innovatie, de transitie naar de 6e cyclus van kondratief, voedselinnovaties en voedseltechnologie. De grote PLOF's
see more on:
wdeheij.blogspot.com
follow @deheij on twitter
Technical information:
www.topwiki.nl
www.top-bv.nl
www.toptechnologytalks.nl
www.foodforfood.info
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
GridMate - End to end testing is a critical piece to ensure quality and avoid...ThomasParaiso2
End to end testing is a critical piece to ensure quality and avoid regressions. In this session, we share our journey building an E2E testing pipeline for GridMate components (LWC and Aura) using Cypress, JSForce, FakerJS…
Threats to mobile devices are more prevalent and increasing in scope and complexity. Users of mobile devices desire to take full advantage of the features
available on those devices, but many of the features provide convenience and capability but sacrifice security. This best practices guide outlines steps the users can take to better protect personal devices and information.
Communications Mining Series - Zero to Hero - Session 1DianaGray10
This session provides introduction to UiPath Communication Mining, importance and platform overview. You will acquire a good understand of the phases in Communication Mining as we go over the platform with you. Topics covered:
• Communication Mining Overview
• Why is it important?
• How can it help today’s business and the benefits
• Phases in Communication Mining
• Demo on Platform overview
• Q/A
Alt. GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using ...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
Removing Uninteresting Bytes in Software FuzzingAftab Hussain
Imagine a world where software fuzzing, the process of mutating bytes in test seeds to uncover hidden and erroneous program behaviors, becomes faster and more effective. A lot depends on the initial seeds, which can significantly dictate the trajectory of a fuzzing campaign, particularly in terms of how long it takes to uncover interesting behaviour in your code. We introduce DIAR, a technique designed to speedup fuzzing campaigns by pinpointing and eliminating those uninteresting bytes in the seeds. Picture this: instead of wasting valuable resources on meaningless mutations in large, bloated seeds, DIAR removes the unnecessary bytes, streamlining the entire process.
In this work, we equipped AFL, a popular fuzzer, with DIAR and examined two critical Linux libraries -- Libxml's xmllint, a tool for parsing xml documents, and Binutil's readelf, an essential debugging and security analysis command-line tool used to display detailed information about ELF (Executable and Linkable Format). Our preliminary results show that AFL+DIAR does not only discover new paths more quickly but also achieves higher coverage overall. This work thus showcases how starting with lean and optimized seeds can lead to faster, more comprehensive fuzzing campaigns -- and DIAR helps you find such seeds.
- These are slides of the talk given at IEEE International Conference on Software Testing Verification and Validation Workshop, ICSTW 2022.
UiPath Test Automation using UiPath Test Suite series, part 5DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 5. In this session, we will cover CI/CD with devops.
Topics covered:
CI/CD with in UiPath
End-to-end overview of CI/CD pipeline with Azure devops
Speaker:
Lyndsey Byblow, Test Suite Sales Engineer @ UiPath, Inc.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
State of ICS and IoT Cyber Threat Landscape Report 2024 previewPrayukth K V
The IoT and OT threat landscape report has been prepared by the Threat Research Team at Sectrio using data from Sectrio, cyber threat intelligence farming facilities spread across over 85 cities around the world. In addition, Sectrio also runs AI-based advanced threat and payload engagement facilities that serve as sinks to attract and engage sophisticated threat actors, and newer malware including new variants and latent threats that are at an earlier stage of development.
The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdfPaige Cruz
Monitoring and observability aren’t traditionally found in software curriculums and many of us cobble this knowledge together from whatever vendor or ecosystem we were first introduced to and whatever is a part of your current company’s observability stack.
While the dev and ops silo continues to crumble….many organizations still relegate monitoring & observability as the purview of ops, infra and SRE teams. This is a mistake - achieving a highly observable system requires collaboration up and down the stack.
I, a former op, would like to extend an invitation to all application developers to join the observability party will share these foundational concepts to build on:
Impact of PEF and thermal processing on apple juice shelf life
1. Short Communication
Impact of PEF and thermal processing on apple juice shelf life
Torkamani AE*
Department of Agriculture and Food systems (DAFS), School of Land and Environment, University of
Melbourne, Melbourne, Vic 3010, Australia.
Received: April 2011, Accepted: July 2011.
ABSTRACT
Background and Objectives: Pulsed electric field (PEF) is a novel emerging technology which is believed to have the
potential to substitute conventional thermal pasteurization (HTST). In the current study PEF was compared with HTST based
on microbial inactivation and quality attributes.
Materials and Methods: Juice was prepared by extracting it from Semirum apples. They were chilled to 4ºC over night.
Then were divided into two lots, one was treated by PEF and the other by HTST. The treated juices were cultured on tryphtic
soy broth (TSB) and results were recorded for 168 days. Quality changes were characterized by color and sensory test. Color
changes were quantified using Hunter Lab equipment and equation. Sensory changes were evaluated by test panelists.
Results: Using selective media E. Coli was enumerated, the total count of the organism was noticeably lower than PEF
treated specimen and after 168. The count didn’t reach the initial population. Whereas in PEF treated juice bacterial count
bounced back to the initial count and exceeds. Results from Hunter Lab indicated a of 3.04 and 3.08 system for PEF and
HTST treated juices. Sensory panel showed that PEF is superior to thermal treatment.
Conclusion: The study indicated HTST is more suitable based on food safety encounters. However PEF treated are closer
to fresh juices based on quality factors. It can be concluded that PEF has the potential to become a suitable replacement to
conventional process if improvements in design are applied.
Keywords: Pulsed Electric field, High temperature low time, Semirum apple, Escherichia coli
INTRODUCTION
With growing consumer interest in healthier and
nutritionally rich food, the juice market has had a major
growth recently. Although thermal processes cause
significant microbial inactivation, many undesired
changes have been reported in different studies
(1). Enzyme deactivation, color change, alterations
in taste and also loss of essential vitamins can be
mentioned (1). Due to the mentioned problems caused
by conventional thermal processes, more tendencies
towards non thermal treatments such as ultrasound,
High hydrostatic pressure, irradiation and Pulsed
Electric Field (PEF) is witnessed. Among these
cold techniques, PEF is growing due to its ability
to inactivate organisms and enzymes while no or
small temperature elevation is recorded leading to
heat sensitive nutrient compound retention (2). Also
sensory quality of PEF treated juice is comparable
to fresh unprocessed juice (2).
Novel methods such as PEF have been introduced
as new processing techniques resulting in improved
product quality (3). PEF effect on multiple quality
factors in treated products has been studied. Qien et al
(4) showed PEF treated apple juice has a shelf life of
maximum 3 weeks. Also another study demonstrated
PEF capability to decrease microorganisms (3).
Regarding to HACCP rules and regulations defined
by FDA, fruit juice processors should attain a 5
logarithmical cycle reduction in the most resistant
organisms counts by the applied techniques (5). The
microbial count should be less than 100 CFU (6).
* Corresponding author: Torkamani AE
Address: Department of Agriculture and Food systems
(DAFS), School of Land and Environment, University of
Melbourne, Melbourne, Vic 3010, Australia.
E-mail: amir_torkamani@hotmail.com
Volume 3 Number 3 (September 2011) 152-155
152
2. 153impact of pef on apple juice
In this study, we studied the effect of PEF on shelf
life in apple juice and compared it with thermal
processed juice. For this purpose we considered
microorganism enumeration as the main determining
factor.
MATERIALS AND METHODS
Apple juice was extracted using a juice extractor
(Mulinex 753 vitafruit) from Semirom apples. Juice
was kept at 4C for 24h then filtered. Pasteurization
was applied using the high temperature-short time
(HTST) method as explained by Moyer and Aitken
(7).
Holding temperature was 74.3 for about 25s. The
process was done using a container to hold the fluids,
coils for juice passage, a pump to circulate juice and
thermocouples as a temperature tracker.
The samples were stored in triplicates in pre-
sterilized glass bottles (30cc) and evaluated for
changes in bacterial count in 14 day periods for 168
days. Bacterial growth count was estimated on tryphtic
soy broth (TSB). Incubation was done at 35ºC . As E.
coli had been demonstrated as the main contaminating
microorganism in breakouts we considered it as the
main pathogen (8). PEF pulses were implemented
with bipolar square wave pulses at 0.01-s pulse
width, 15 Hz frequency. The device consisted of a
probe (P6014A, Tekronix) and a digital oscilloscope
(Tekronix). 220 V AC power was converted to 30
kV AC by a transformer and then regenerated to
high voltage DC. Flow rate was between 5-50 ml/
sec, controlled by a peristaltic pump. The fluid was
processed for 1 μs. The processed juice was then
kept in 3 samples in sterile glass bottles (30cc) and
bacterial growth was evaluated as explained before.
Sensorytest.Inordertoevaluatethesensoryspecifica-
tion and alteration of the samples, the triangle differenc-
es test was performed. A number of panelists, mostly
students, were invited and provided with the samples.
The juice was settled in opaque glass containers.
Color. The color of the treated and control samples
was evaluated using hunterlab spectrophotometer
(Hunterlab Colorflex, Reston, Virginia, USA). The
system iluminant/observer was set at D65/10 which is
recommended by the manufacturer and was operated
at ambient temperature. The device was calibrated
with black and white reference glasses. Samples were
transferred to cuvets and data were recorded.
Color difference, ΔE, is calculated by the following
equation. (Equation 1)
Where Δa, Δb and ΔL are difference in red/green,
yellow/blue and light/dark ranges respectively. Table
1 explains ΔE based on Cserhalmi et al. work (9).
Obtained data were analyzed using SPSS 18
software. In order to determine difference between
remaining CFU during storage data were rendered by
analysis of variance (ANOVA) at significance level
of p0.05 .
RESULTS
To evaluate the processing techniques on shelf life,
we estimated the bacterial counts in the samples in
14 day intervals. Fig 1 demonstrates these counts.
After analyzing the obtained data a P value of 0.034
was calculated when comparing thermal processing
with PEF. Also a mean log count of 0.56 and 1.89
was estimated for thermal and PEF treated juice
respectively.
ColortestresultsobtainedfromHunterLabdeviceis
represented in Table 1. L is the lightness, a*represents
red to green range and b* ranges between yellow and
Fig. 1. E.coli total count during storage.
(Eq. 1)
Difference in color
0-0.5 Not noticeable
0.5-1.5 Slightly noticeable
1.5-3.0 Noticeable
3.0-6.0 Well visible
6.0-12.0 Great
Table 1. classification.
3. 154 Torkamani ETAL . IRAN. J. MICROBIOL. 3 (3) : 152-155
blue (10). L value ranges between 0 and 100 where
zero is black and 100 is diffuse white. Negative
values for a* represent green while positive
indicates red/magnita. Negative range indicates
blue color while positive interval determines
yellow dominance (10).
We considered a p value less than 0.05 as significant.
This was considered as equivalent to about 9 to 15
validated answers out of 18 testers (11). Our results
showed that 11 out of 18 testers indicated that the
PEF treated juice had better quality attributes than
thermally treated samples.
CONCLUSIONS
As explained, difference in bacterial count between
the two methods was meaningful (P value: 0.034)
showing that thermal processing is significantly more
efficient in bacterial decrease than PEF. It can be
depicted from Fig. 1 that bacterial counts decrease
in the first 48 days but after that the organisms start
the recovery process. Results show zero counts at
the 48th
day for HTST treated samples. This may be
due to the state called “viable but nonculturable cells
(VBNC)” where cells do not grow on plates due to
metabolical injury (12). In order to detect and count
these cells, selective media should be used (12). As
TSB is a selective media for enriching fecal coliforms
enumeration was possible.
Although the conventional heat processing method
was more effective, PEF treated samples constantly
had a log count < 2.0 that is also acceptable according
to marketing standards (6).
Generally when apple juice undergoes Millard brown-
ing reactions L value decreases, b* and a* increases
(10). This means that the juice becomes darker and
more yellow and red. As it is shown in Table 2 the
PEF treated juice is lighter, greener and bluer than
both fresh and HTST treated juices. Fresh juice has
better visual quality attributes than thermally treated
juice but less than PEF treated specimens according
to Table 2. Calculation quantifies visual
significance in juice quality. value for PEF and
HTST are calculated as 3.04 and 3.08 which are in the
well visible range (Table 1). Triangle test indicated
significant difference between PEF and HTST treated
juice in taste.
This study showed that, PEF treated juice had no
difference in quality from control samples (fresh
juice). Our results demonstrated that although HTST
is more efficient in microorganism reduction, PEF is
an effective and acceptable method, not only suitable
for bacterial reduction but also resulting in better
flavor and color preservation.
Acknowledgement
The author’s appreciation goes to the Wageningen
University and Research center (WUR), the
Netherlands which part of this study was conducted at
the department of food and bioprocess engineering.
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