This document discusses cell perforation using pulsed electric fields (PEF) to preserve fresh juice. It notes that microbes are naturally present in fresh juice and can exponentially grow, potentially causing foodborne illness outbreaks. The document then explains that applying a pulsed electric field to the juice using PEF technology creates an electric field distribution that perforates microbe cell membranes, immediately eliminating their viability. The result is healthy juice with an extended shelf-life of 21-60 days without refrigeration.

1. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
PurePulse for Dummies - 1
Background of cell perforation using PEF
Myriam Knopf
Application Technologist PEF
www.top-bv.nl
www.toptechnologytalks.nl
www.tophealthfacts.nl
www.topfoodlab.nl
www.topwiki.nl

2. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
Background – freshly squeezed, tasty & healthy

3. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
Unfortunnaly microbes are present in the juice …

4. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
And if we do nothing, exponential growth occurs

5. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
Even outbreaks due to pathogens may occur …
Pathogeen Jaar Aantal zieken Aantal doden Oorzaak Land
Salmonella
Typhimurium
1944 18 1 Restaurant medewerker
(zelf geen symptomen)
Cleveland, Ohio, US
Enterogene E. coli 1992 6 0 Slechte hygiëne
verkopers
India
Salmonella Hartford,
Gaminara, Rubislaw
1995 63, 7 opgenomen
ziekenhuis
0 Amfibieën (padden) Orlando, Florida, US
Shigella flexneri 1995 14 - geen verdere details Zuid-Afrika
Salmonella enterica 1999 500 0 Sinaasappels besmet Adelaide, Australië
Salmonella muenchen 1999 423 1 Mexicaans
sinaasappelsap
Tempe, Arizona, US
Salmonella Anatum 1999 6 0 Straat verkoper, slechte
hygiëne.
Florida, US
Salmonella
Typhimurium
1999 405 0 Pathogeen ongeopende
verpakkingen
Australie
Salmonella enteritidis 2000 74 0 Bron is niet bekend. Calfornia, US
Salmonella
Typhimurium en
SaintPaul
2005 152 - HACCP slecht
gehandhaaft
Meerdere staten, US
Salmonella Panama 2008 15 0 Besmet vruchtensap Nederland

6. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
But, suppose we put a penlight on the juice …
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7. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
… that provides an electric field distribution …
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8. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
.. due to this E-field, the membrane perforates ..
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9. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
.. and the microbes viability is gone immediately
R.I.P.
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10. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013
The result? Healthy juice with sufficient shelf-life
21-60 days